Ultra-sensitive DNA assay based on single-molecule detection coupled with fluorescent quantum dot-labeling and its application to determination of messenger RNA

Analytica Chimica Acta

Volumn 685, Issue 1, 2011, Pages 52-57

  Li, Li ^a   Li, Xincang ^a   Li, Lu ^a   Wang, Jinxing ^a   Jin, Wenrui ^a  

^a SHANDONG UNIVERSITY   (China)

Author keywords

Deoxyribonucleic acid determination; Messenger ribonucleic acid determination; Single molecule detection; Total internal reflection fluorescence microscopy

Indexed keywords

ANALYTES; BOVINE SERUM ALBUMINS; BRIGHT SPOTS; CALIBRATION CURVES; CELLULAR MRNAS; DEOXYRIBONUCLEIC ACIDS; DNA ASSAYS; DOUBLE-STRANDED RNA; ETHANOLAMINES; FLUORESCENCE PROBES; FLUORESCENT IMAGES; GLASS COVERSLIPS; IN-CELL; KEY FACTORS; MESSENGER RNA; MESSENGER RNAS (MRNA); NOISE ELIMINATION; NONSPECIFIC ADSORPTION; OSTEOPONTIN; PHASE DETECTION; QUANTUM DOT; QUANTUM DOTS; REVERSE TRANSCRIPTASES; REVERSE TRANSCRIPTION; RIBONUCLEIC ACID; SINGLE-MOLECULE DETECTION; STROMAL CELLS; SUBSTRATE PREPARATION; TOTAL INTERNAL REFLECTION FLUORESCENCE MICROSCOPY;

ADSORPTION; BODY FLUIDS; ENZYME ACTIVITY; FLUORESCENCE; FLUORESCENCE MICROSCOPY; MOLECULES; ORGANIC ACIDS; PROBES; REFRACTIVE INDEX; RNA; SEMICONDUCTOR QUANTUM DOTS;

DNA;

BOVINE SERUM ALBUMIN; COMPLEMENTARY DNA; DOUBLE STRANDED RNA; ETHANOLAMINE; FLUORESCENT DYE; MESSENGER RNA; QUANTUM DOT; RNA DIRECTED DNA POLYMERASE;

ADSORPTION; ARTICLE; BINDING KINETICS; DNA DETERMINATION; DNA HYBRIDIZATION; DNA SYNTHESIS; FLUORESCENCE MICROSCOPY; PRIORITY JOURNAL; REVERSE TRANSCRIPTION;

ANIMALS; CATTLE; DNA; DNA, COMPLEMENTARY; MICROSCOPY, FLUORESCENCE; NUCLEIC ACID HYBRIDIZATION; QUANTUM DOTS; RNA, MESSENGER; SENSITIVITY AND SPECIFICITY;

BOVINAE;

EID: 78650179469     PISSN: 00032670     EISSN: 18734324     Source Type: Journal    
DOI: 10.1016/j.aca.2010.11.012     Document Type: Article

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