Changes in sperm apoptotic markers as related to seminal leukocytes and elastase

Reproductive BioMedicine Online

Volumn 21, Issue 1, 2010, Pages 84-92

  Zorn, B ^a   Ihan, A ^b   Kopitar, A N ^b   Kolbezen, M ^a   Sesek Briski A ^a   Meden Vrtovec, H ^a  

^a UNIVERSITY MEDICAL CENTRE LJUBLJANA   (Slovenia)

^b UNIVERSITY OF LJUBLJANA   (Slovenia)

Author keywords

DNA denaturation; elastase; intracellular ROS; mitochondrial membrane potential; necrosis; spermatozoa

Indexed keywords

BIOLOGICAL MARKER; DNA; ELASTASE; PHOSPHOLIPID; REACTIVE OXYGEN METABOLITE;

ADULT; APOPTOSIS; ARTICLE; CELL MEMBRANE; CELL STRUCTURE; CONTROLLED STUDY; CYTOPLASM; DENSITY GRADIENT; DNA DENATURATION; DNA INTEGRATION; FLOW CYTOMETRY; HUMAN; HUMAN CELL; INTRACELLULAR MEMBRANE; LEUKOCYTE; MAJOR CLINICAL STUDY; MALE; MALE INFERTILITY; MITOCHONDRIAL MEMBRANE POTENTIAL; SEMINAL PLASMA; SPERM; SPERMATOZOON COUNT; SPERMATOZOON DENSITY; SPERMATOZOON MATURATION; SPERMATOZOON MOTILITY; VARICOCELE;

ADULT; APOPTOSIS; BIOLOGICAL MARKERS; DNA; HUMANS; INFERTILITY, MALE; LEUKOCYTE COUNT; MALE; MEMBRANE POTENTIAL, MITOCHONDRIAL; MIDDLE AGED; NECROSIS; NUCLEIC ACID DENATURATION; PANCREATIC ELASTASE; REACTIVE OXYGEN SPECIES; SEMEN; SPERM COUNT; SPERM MOTILITY; SPERMATOZOA;

EID: 78650169464     PISSN: 14726483     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.rbmo.2010.03.016     Document Type: Article

References (54)

1. R.J. Aitken, K. West, and D. Buckingham Leukocytic infiltration into the human ejaculate and its association with semen quality, oxidative stress, and sperm function J. Androl. 15 1994 343 352 (Pubitemid 24256649)
2. S. Altairac, S. Zeggai, and P. Perani Apoptosis induced by Na+/H+ antiport inhibition activates the LEI/L-DNase II pathway Cell Death Differ. 10 2003 548 557 (Pubitemid 36656644)
3. J.G. Alvarez, R.K. Sharma, and M. Ollero Increased DNA damage in sperm from leukocytospermic semen samples as determined by the sperm chromatin structure assay Fertil. Steril. 78 2002 319 329 (Pubitemid 34831733)
4. G. Barroso, M. Morshedi, and S. Oehninger Analysis of DNA fragmentation, plasma membrane translocation of phosphatidylserine and oxidative stress in human spermatozoa Hum. Reprod. 15 2000 1338 1344 (Pubitemid 30335960)
5. G. Barroso, S. Taylor, and M. Morshedi Mitochondrial membrane potential integrity and plasma membrane translocation of phosphatidylserine as early apoptotic markers: a comparison of two different sperm subpopulations Fertil. Steril. 85 2006 149 154 (Pubitemid 43063438)
6. A. Borini, N. Tarozzi, and D. Bizzaro Sperm DNA: paternal effect on early post-implantation embryo development in ART Hum. Reprod. 21 2006 2876 2881 (Pubitemid 44567001)
7. Z. Chen, R. Hauser, A.M. Trbovich, J.L. Shifren, D.J. Dorer, L. Godfrey-Bailey, and N.P. Singh The relationship between human sperm characteristics and sperm apoptosis: a pilot study J. Androl. 27 2006 112 120 (Pubitemid 43106743)
8. G. Collodel, B. Baccetti, and S. Capitani Necrosis in human spermatozoa. I. Ultrastructural features and FISH study in semen from patients with uro-genital infections J. Submicrosc. Cytol. Pathol. 37 2005 67 73 (Pubitemid 41098068)
9. F. Comhaire, G. Verschraegen, and L. Vermeulen Diagnosis of accessory gland infection and its possible role in male infertility Int. J. Androl. 3 1980 32 45 (Pubitemid 10217376)
10. E.T. Donnelly, M. O'Connell, N. McClure, and S.E.M. Lewis Differences in nuclear DNA fragmentation and mitochondrial integrity of semen and prepared human spermatozoa Hum. Reprod. 15 2000 1552 1561
11. W. Eggert-Kruse, I. Kiefer, and C. Beck Role for tumor necrosis factor alpha (TNF-alpha) and interleukin 1-beta (IL-1beta) determination in seminal plasma during infertility investigation Fertil. Steril. 87 2007 810 823 (Pubitemid 46550410)
12. A. Eley, S. Hosseinzadeh, and H. Hakimi Apoptosis of ejaculated human sperm is induced by co-incubation with Chlamydia trachomatis lipopolysaccharide Hum. Reprod. 20 2005 2601 2607 (Pubitemid 41222516)
13. A.W. Endtz A rapid staining method for differentiating granulocytes from 'germinal cells' in Papanicolaou-stained semen Acta Cytol. 18 1974 2 7
14. D. Evenson, and R. Wixon Meta-analysis of sperm DNA fragmentation using the sperm chromatin structure assay Reprod. Biomed. Online 12 2006 466 472
15. D.P. Evenson, K.L. Larson, and L.K. Jost Sperm chromatin structure assay: its clinical use for detecting sperm DNA fragmentation in male infertility and comparisons with other techniques J. Androl. 23 2002 25 43 (Pubitemid 34001126)
16. T.A. Fuchs, U. Abed, and C. Goosmann Novel cell death program leads to neutrophil extracellular traps J. Cell. Biol. 176 2007 231 241 (Pubitemid 46143263)
17. G. Gallegos, B. Ramos, and R. Santiso Sperm DNA fragmentation in infertile men with genitourinary infection by Chlamydia trachomatis and Mycoplasma Fertil. Steril. 90 2007 328 334
18. L. Gandini, F. Lombardo, and D. Paoli Study of apoptotic DNA in human spermatozoa Hum. Reprod. 15 2000 830 839 (Pubitemid 30192731)
19. W. Gorczyca, F. Traganos, H. Jesionowska, and Z. Darzynkiewicz Presence of DNA strand breaks and increased sensitivity of DNA in situ to denaturation in abnormal human sperm cells: analogy to apoptosis of somatic cells Exp. Cell Res. 207 1993 202 205 (Pubitemid 23192183)
20. G. Grizard, L. Ouchchane, H. Roddier, C. Artonne, B. Sion, M.P. Vasson, and L. Janny In vitro alachlor effects on reactive oxygen species generation, motility patterns and apoptosis markers in human spermatozoa Reprod. Toxicol. 23 2007 55 62 (Pubitemid 46027536)
21. S. Grunewald, T.M. Said, U. Paasch, H.J. Glander, and A. Agarwal Relationship between sperm apoptosis signalling and oocyte penetration capacity Int. J. Androl. 31 2008 325 330 (Pubitemid 351652027)
22. G. Haidl, J.P. Allam, and H.C. Schuppe Chronic epididymitis: impact on semen parameters and therapeutic options Andrologia 40 2008 92 96 (Pubitemid 351379664)
23. R. Henkel, and W.B. Schill Sperm separation in patients with urogenital infections Andrologia 30 Suppl. 1 1998 91 97 (Pubitemid 28244199)
24. R. Henkel, E. Kierspel, and T. Stalf Effect of reactive oxygen species produced by spermatozoa and leukocytes on sperm functions in non-leukocytospermic patients Fertil. Steril. 83 2005 635 642 (Pubitemid 40348575)
25. M. Jochum, W. Pabst, and W.B. Schill Granulocyte elastase as a sensitive diagnostic parameter of silent male genital tract inflammation Andrologia 18 1986 413 419 (Pubitemid 16009504)
26. J. Kratzsch, U. Paasch, and S. Grunewald Resistin correlates with elastase and interleukin-6 in human seminal plasma Reprod. Biomed. Online 16 2008 283 288 (Pubitemid 351291978)
27. M. Maegawa, M. Kamada, and M. Irahara Concentration of granulocyte elastase in seminal plasma is not associated with sperm motility Arch. Androl. 47 2001 31 36 (Pubitemid 32579155)
28. C. Marchetti, G. Obert, and A. Deffosez Study of mitochondrial membrane potential, reactive oxygen species, DNA fragmentation and cell viability by flow cytometry in human sperm Hum. Reprod. 17 2002 1257 1265 (Pubitemid 34537360)
29. A. Moriyama, K. Shimoya, and A. Kawamoto Secretory leukocyte protease inhibitor (SLP) concentrations in seminal plasma: SLPI restores sperm motility reduced by elastase Mol. Hum. Reprod. 4 1998 946 950 (Pubitemid 28475249)
30. G.J.E. Oosterhuis, A.B. Mulder, and E. Kalsbeek-Batenburg Measuring apoptosis in human spermatozoa: a biological assay for semen quality? Fertil. Steril. 74 2000 245 250 (Pubitemid 30604185)
31. U. Paasch, A. Agarwal, and A.K. Gupta Apoptosis signal transduction and the maturity status of human spermatozoa Ann. NY Acad. Sci. 1010 2003 486 488 (Pubitemid 38265030)
32. G. Ricci, S. Perticarari, and R. Boscolo Semen preparation methods and sperm apoptosis: swim-up versus gradient-density centrifugation technique Fertil. Steril. 91 2009 632 638
33. G. Ricci, S. Perticarari, and E. Fragonas Apoptosis in human sperm: its correlation with semen quality and the presence of leukocytes Hum. Reprod. 17 2002 2665 2672 (Pubitemid 35174120)
34. Rowe, P.J., Comhaire, F.H., Hargreave, T.B., et al., 2000. Objective criteria for diagnostic categories in the standardized management of male infertility: male accessory gland infection (MAGI). In: Rowe, P.J., Comhaire, F.H., Hargreave, T.B., Mahmoud, A.M.A. (Eds.), WHO Manual for the Standardized Investigation, Diagnosis and Management of the Infertile Male, first ed. World Health Organization, Cambridge University Press, Cambridge, UK, pp. 52-54.
35. D. Sakkas, E. Seli, and D. Bizzaro Abnormal spermatozoa in the ejaculate: abortive apoptosis and faulty nuclear remodelling during spermatogenesis Reprod. Biomed. Online 7 2003 428 432 (Pubitemid 37441699)
36. D. Sakkas, F. Urner, and D. Bizzaro Sperm nuclear DNA damage and altered chromatin structure: effect on fertilization and embryo development Hum. Reprod. 13 Suppl. 4 1998 11 19 (Pubitemid 29086087)
37. R.A. Saleh, A. Agarwal, and E.A. Nada Negative effects of increased sperm DNA damage in relation to seminal oxidative stress with idiopathic and male factor infertility Fertil. Steril. 79 Suppl. 3 2003 1597 1605 (Pubitemid 36687939)
38. A. Satta, A. Stivala, and A. Garozzo Experimental Chlamydia trachomatis infection causes apoptosis in human sperm Hum. Reprod. 21 2006 134 137 (Pubitemid 41794301)
39. R.K. Sharma, F.F. Pasqualotto, D.R. Nelson, A.J. Thomas Jr., and A. Agarwal Relationship between seminal white blood cell counts and oxidative stress in men treated at an infertility clinic J. Androl. 22 2001 575 582
40. M.J. Tomlinson, A. White, and C.I.R. Barratt The removal of morphologically abnormal sperm forms by phagocytes: a positive role for seminal leukocytes? Hum. Reprod. 7 1992 517 522
41. L. Troiano, A.R. Granata, and A. Cossarizza Mitochondrial membrane potential and DNA stainability in human sperm cells: a flow cytometry with implications for male infertility Exp. Cell Res. 15 1998 384 393 (Pubitemid 28366564)
42. J. Villegas, M. Schulz, and L. Soto Influence of reactive oxygen species produced by activated leukocytes at the level of apoptosis in mature human spermatozoa Fertil. Steril. 83 2005 808 810 (Pubitemid 40348609)
43. M.R. Virro, K.L. Larson-Cook, and D.P. Evenson Sperm chromatin structure assay (SCSA®) parameters are related to fertilization, blastocyst development, and ongoing pregnancy in in vitro fertilization and intracytoplasmic sperm injection cycles Fertil. Steril. 81 2004 1289 1295 (Pubitemid 38609313)
44. X. Wang, R.K. Sharma, and A. Gupta Alterations in mitochondrial membrane potential and oxidative stress in infertile men: a prospective observational study Fertil. Steril. 80 Suppl. 2 2003 844 850 (Pubitemid 37152403)
45. H. Wolff, J.A. Politch, and A. Martinez Leukocytospermia is associated with poor semen quality Fertil. Steril. 53 1990 528 536 (Pubitemid 20093619)
46. World Health Organization WHO Laboratory Manual for the Examination of Human Semen and Semen-Cervical Mucus Interaction fourth ed. 1999 Cambridge University Press Cambridge, UK pp. 128
47. E.H. Yanushpolsky, J.A. Politch, and J.A. Hill Antibiotic therapy and leukocytospermia: a prospective, randomized, controlled study Fertil. Steril. 63 1995 142 147
48. A. Zini, R. Bielecki, and D. Phang Correlations between two markers of sperm DNA integrity, DNA denaturation and DNA fragmentation, in fertile and infertile men Fertil. Steril. 75 2001 674 677 (Pubitemid 32273501)
49. A. Zini, V. Mak, D. Phang, and K. Jarvi Potential adverse effects of sperm processing on human sperm deoxyribonucleic acid integrity Fertil. Steril. 72 1999 496 499 (Pubitemid 29414891)
50. A. Zini, J. Meriano, and K. Kader Potential adverse effect of sperm DNA damage on embryo quality after ICSI Hum. Reprod. 20 2005 3476 3480 (Pubitemid 41742530)
51. A. Ziyyat, V. Barraud-Lange, C. Sifer, B. Ducot, J.P. Wolf, and J.C. Soufir Paradoxical increase of sperm motility and seminal carnitine associated with moderate leukocytospermia in infertile patients Fertil. Steril. 90 2008 2257 2263
52. A. Zöpfgen, F. Priem, and F. Sudhoff Relationship between semen quality and the seminal plasma components carnitine, alpha-glucosidase, fructose, citrate and granulocyte elastase in infertile men compared with a normal population Hum. Reprod. 15 2000 840 845 (Pubitemid 30192732)
53. B. Zorn, I. Virant-Klun, and H. Meden-Vrtovec Semen granulocyte elastase: its relevance for the diagnosis and prognosis of silent genital tract inflammation Hum. Reprod. 15 2000 1978 1984
54. B. Zorn, I. Virant-Klun, and G. Vidmar Seminal elastase-inhibitor complex, a marker of genital tract inflammation, and negative IVF outcome measures: a role for a silent inflammation? Int. J. Androl. 27 2004 368 374 (Pubitemid 39665395)