Measuring apoptosis in human spermatozoa: A biological assay for semen quality?

Fertility and Sterility

Volumn 74, Issue 2, 2000, Pages 245-250

  Oosterhuis, G Jurjen E ^a   Mulder, Andre B ^a   Kalsbeek Batenburg, Ellen ^a   Lambalk, Cornelis B ^b   Schoemaker, Joop ^b   Vermes, Istvan ^a  

^a MEDISCH SPECTRUM TWENTE   (Netherlands)

^b ACADEMIC MEDICAL CENTER   (Netherlands)

Author keywords

Annexin V assay; Apoptosis; Male factor infertility; Spermatozoa; TUNEL assay

Indexed keywords

DNA FRAGMENT; LIPOCORTIN 5; PHOSPHATIDYLSERINE;

APOPTOSIS; ARTICLE; BIOASSAY; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; DIAGNOSTIC VALUE; FLOW CYTOMETRY; HUMAN; HUMAN CELL; MAJOR CLINICAL STUDY; MALE; MALE INFERTILITY; NICK END LABELING; PRIORITY JOURNAL; SEMEN ANALYSIS; SPERMATOZOON; SPERMATOZOON DENSITY;

ANNEXIN A5; APOPTOSIS; DNA FRAGMENTATION; FLOW CYTOMETRY; FLUORESCEIN-5-ISOTHIOCYANATE; FLUORESCENT DYES; HUMANS; IN SITU NICK-END LABELING; INFERTILITY, MALE; MALE; PHOSPHATIDYLSERINES; PILOT PROJECTS; PROPIDIUM; SPERM MOTILITY; SPERMATOZOA;

EID: 0034254640     PISSN: 00150282     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0015-0282(00)00623-3     Document Type: Article

References (18)

1. WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. 3rd ed. 1992;Cambridge University Press, Cambridge, UK.
2. Hikim A.P., Wang C., Lue Y., Johnson L., Wang X.H., Swerdloff R.S. Spontaneous germ cell apoptosis in humans evidence for ethnic differences in the susceptibility of germ cells to programmed cell death . J Clin Endocrinol Metab. 83:1998;152-156.
3. Erkkila K., Hirvonen V., Wuokko E., Parvinen M., Dunkel L. N-acetyl-L-cysteine inhibits apoptosis in human male germ cells in vitro. J Clin Endocrinol Metab. 83:1998;2523-2531.
4. Koopman G., Reutelingsperger C.P.M., Kuijten G.A.M., Keehnen R.M.J., Pals S.T., van Oers M.H.J. Annexin V for flow cytometric detection of phosphatidylserine expression of B cells undergoing apoptosis. Blood. 84:1994;1415-1420.
5. Andree H.A., Reutelingsperger C.P.M., Hauptmann R., Hemker H.C., Hermens W.T., Willems G.M. Binding of vascular anticoagulant alpha (VAC alpha) to planar phospholipid bilayers. J Biol Chem. 265:1990;4923-4928.
6. Meers P., Mealy T. Relationship between Annexin V tryptophan exposure, calcium, and phospholipid binding. Biochemistry. 32:1993;5411-5418.
7. Jeyendran R.S., Van der Ven H.H., Perez-Pelaez M., Crabo B.G., Zaneveld L.J.D. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J Reprod Fertil. 70:1984;219-228.
8. Oosterhuis G.J.E., Hampsink R.M., Michgelsen H.W.B., Vermes I. Hypo-osmotic swelling test a reliable screening assay for routine semen specimen quality screening . J Clin Lab Anal. 10:1996;209-212.
9. Vermes I., Haanen C., Steffens-Nakken H., Reutelingsperger C.P.M. A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J Immunol Methods. 184:1995;39-51.
10. Overbeeke R., Steffens-Nakken H., Vermes I., Reutelingsperger C.P.M., Haanen C. Early features of apoptosis detected by four different flow cytometry assays. Apoptosis. 3:1998;115-121.
11. Gavrieli Y., Sherman Y., Ben-Sasson S.A. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J Cell Biol. 119:1992;493-501.
12. Yang J.H., Wu M.Y., Chao K.H., Chen S.U., Ho H.N., Yang Y.S. Controlled ovarian hyperstimulation and intrauterine insemination in subfertility. How many treatment cycles are sufficient? J Reprod Med. 43:1998;903-908.
13. Dickey R.P., Pyrzak R., Lu P.Y., Taylor S.N., Rye P.H. Comparison of the sperm quality necessary for successful intrauterine insemination with World Health Organization threshold values for normal sperm. Fertil Steril. 71:1999;684-689.
14. Rotter V., Schwartz D., Almon E., Goldfinger N., Kapon A., Meshorer A., et al. Mice with reduced levels of p53 protein exhibit the testicular giant- cell degenerative syndrome. Proc Natl Acad Sci U S A. 90:1993;9075-9079.
15. Knudson C.M., Tung K.S., Tourtellotte W.G., Brown G.A., Korsmeyer S.J. Bax-deficient mice with lymphoid hyperplasia and male germ cell death. Science. 270:1995;96-99.
16. Lee H.W., Blasco M.A., Gottlieb G.J., Horner J.W., Greider C.W., DePinho R.A. Essential role of mouse telomerase in highly proliferative organs. Nature. 392:1998;569-574.
17. Glander H.J., Schaller J. Binding of Annexin V to plasma membranes of human spermatozoa a rapid assay for detection of membrane changes after cryostorage . Mol Hum Reprod. 5:1999;109-115.
18. Wyllie A.H., Kerr J.F.R., Currie A.R. Cell death the significance of apoptosis . Int Rev Cytol. 68:1980;251-300.