Brain tumor stem cells maintain overall phenotype and tumorigenicity after in vitro culturing in serum-free conditions

Neuro-Oncology

Volumn 12, Issue 12, 2010, Pages 1220-1230

  Vik Mo, Einar Osland ^a,b   Sandberg, Cecilie ^a   Olstorn, Havard ^a,b   Varghese, Mercy ^a,b   Brandal, Petter ^a   Ramm Pettersen, Jon ^a   Murrell, Wayne ^a   Langmoen, Iver Arne ^a,b  

^a OSLO UNIVERSITY HOSPITAL   (Norway)

^b ULLEVÅL UNIVERSITY HOSPITAL   (Norway)

Author keywords

Adaptation; Brain tumor stem cell; Culture media; Glioblastoma multiforme; Serum free; Tumor sphere

Indexed keywords

BETA TUBULIN; TRANSCRIPTOME;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CANCER STEM CELL; CARCINOGENICITY; CELL DIFFERENTIATION; CHROMOSOME ABERRATION; COMPARATIVE GENOMIC HYBRIDIZATION; CONTROLLED STUDY; GENETIC VARIABILITY; GLIOBLASTOMA; HUMAN; IMMUNOCYTOCHEMISTRY; IN VITRO STUDY; KARYOTYPING; MOUSE; NONHUMAN; OUTCOME ASSESSMENT; PHENOTYPE; TUMOR BIOPSY; TUMOR DIFFERENTIATION; TUMOR GROWTH;

ANIMALS; ASTROCYTOMA; BRAIN NEOPLASMS; CELL CULTURE TECHNIQUES; CHROMOSOME ABERRATIONS; COMPARATIVE GENOMIC HYBRIDIZATION; CULTURE MEDIA, SERUM-FREE; DNA, NEOPLASM; GENE EXPRESSION PROFILING; HUMANS; KARYOTYPING; MICE; MICE, SCID; NEOPLASTIC STEM CELLS; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PHENOTYPE; POLYMERASE CHAIN REACTION; RNA, NEOPLASM; SIGNAL TRANSDUCTION; TRANSPLANTATION, HETEROLOGOUS; TUMOR CELLS, CULTURED; TUMOR MARKERS, BIOLOGICAL;

EID: 78650126756     PISSN: 15228517     EISSN: 15235866     Source Type: Journal    
DOI: 10.1093/neuonc/noq102     Document Type: Article

References (37)

1. Stupp R, Hegi ME, Mason WP, et al. Effects of radiotherapy withconcomitant and adjuvant temozolomide versus radiotherapy aloneon survival in glioblastoma in a randomised phase III study: 5-yearanalysis of the EORTC-NCIC trial. Lancet Oncol. 2009;10:459-466.
2. Reynolds BA, Weiss S. Generation of neurons and astrocytes from isolatedcells of the adult mammalian central nervous system. Science.1992;255:1707-1710.
3. Galli R, Binda E, Orfanelli U, et al. Isolation and characterization oftumorigenic, stem-like neural precursors from human glioblastoma.Cancer Res. 2004;64:7011-7021.
4. Hemmati HD, Nakano I, Lazareff JA, et al. Cancerous stem cells canarise from pediatric brain tumors. Proc Natl Acad Sci USA.2003;100:15178-15183.
5. Ignatova TN, Kukekov VG, Laywell ED, Suslov ON, Vrionis FD, SteindlerDA. Human cortical glial tumors contain neural stem-like cellsexpressing astroglial and neuronal markers in vitro. Glia.2002;39:193-206.
6. Singh SK, Clarke ID, Terasaki M, et al. Identification of a cancer stemcell in human brain tumors. Cancer Res. 2003;63:5821-5828.
7. Varghese M, Olstorn H, Sandberg C, et al. A comparison between stemcells from the adult human brain and from brain tumors. Neurosurgery.2008;63:1022- 1033.
8. Yuan X, Curtin J, Xiong Y, et al. Isolation of cancer stem cells from adultglioblastoma multiforme. Oncogene. 2004;23:9392-9400.
9. Laks DR, Masterman-Smith M, Visnyei K, et al. Neurosphere formationis an independent predictor of clinical outcome in malignant glioma.Stem Cells. 2009;27:980-987.
10. Pallini R, Ricci-Vitiani L, Banna GL, et al. Cancer stem cell analysis andclinical outcome in patients with glioblastoma multiforme. Clin CancerRes. 2008;14:8205-8212.
11. Al-Hajj M, Wicha MS, ito-Hernandez A, Morrison SJ, Clarke MF.Prospective identification of tumorigenic breast cancer cells. Proc NatlAcad Sci USA. 2003;100:3983-3988. (Pubitemid 36418143)
12. Gibbs CP, Kukekov VG, Reith JD, et al. Stem-like cells in bone sarcomas:implications for tumorigenesis. Neoplasia. 2005;7:967-976.
13. Guo S, Liu T, Wang C, et al. Establishment of clonal colony-formingassay for propagation of pancreatic cancer cells with stem cell properties.Pancreas. 2007;34:429-435.
14. Beier D, Hau P, Proescholdt M, et al. CD133(+) and CD133(2)glioblastoma- derived cancer stem cells show differential growthcharacteristics and molecular profiles. Cancer Res.2007;67:4010-4015.
15. Gunther HS, Schmidt NO, Phillips HS, et al. Glioblastoma-derived stemcell-enriched cultures form distinct subgroups according to molecularand phenotypic criteria. Oncogene. 2008;27:2897-2909.
16. Salmaggi A, Boiardi A, Gelati M, et al. Glioblastoma-derived tumorospheresidentify a population of tumor stem-like cells with angiogenicpotential and enhanced multidrug resistance phenotype. Glia.2006;54:850-860.
17. Lee J, Kotliarova S, Kotliarov Y, et al. Tumor stem cells derived from glioblastomascultured in bFGF and EGF more closely mirror the phenotypeand genotype of primary tumors than do serum-cultured cell lines.Cancer Cell. 2006;9:391-403.
18. Li A, Walling J, Kotliarov Y, et al. Genomic changes and gene expressionprofiles reveal that established glioma cell lines are poorly representativeof primary human gliomas. Mol Cancer Res. 2008;6:21-30.
19. Mandahl N. Methods in solid rumors cytogenetics. In: Rooney DE,Czepulkowski BH eds. Human Cytogenetics: A Practical Approach.Vol. 2, Malignancy and Acquired Chromosome Abnormalities.Eynsham, Oxon: IRL Press; 1992:155-187.
20. Schaffer LG, Tommerup N. ISCN: an International System for HumanCytogenetic Nomenclature. Basel, Switzerland: S. Karger; 2005.
21. Teixeira MR, Ribeiro FR, Eknaes M, et al. Genomic analysis of prostatecarcinoma specimens obtained via ultrasound-guided needle biopsymay be of use in preoperative decision-making. Cancer.2004;101:1786-1793.
22. Ribeiro FR, Jeronimo C, Henrique R, et al. 8q gain is an independentpredictor of poor survival in diagnostic needle biopsies from prostatecancer suspects. Clin Cancer Res. 2006;12:3961-3970.
23. Serra-Pages C, Kedersha NL, Fazikas L, Medley Q, Debant A, Streuli M.The LAR transmembrane protein tyrosine phosphatase and a coiled-coilLAR-interacting protein co-localize at focal adhesions. EMBO J.1995;14:2827-2838.
24. Moe MC, Varghese M, Danilov AI, et al. Multipotent progenitor cellsfrom the adult human brain: neurophysiological differentiation tomature neurons. Brain. 2005;128:2189-2199.
25. Fael Al-Mayhani TM, Ball SL, Zhao JW, et al. An efficient method forderivation and propagation of glioblastoma cell lines that conservesthe molecular profile of their original tumours. J Neurosci Methods.2009;176:192- 199.
26. Varghese M, Olstorn H, Berg-Johnsen J, Moe MC, Murrell W,Langmoen IA. Isolation of human multipotent neuralprogenitors from adult filum terminale. Stem Cells Dev.2009;18:603-613.
27. Ernst A, Hofmann S, Ahmadi R, et al. Genomic and expression profilingof glioblastoma stem cell-like spheroid cultures identifies novel tumorrelevantgenes associated with survival. Clin Cancer Res.2009;15:6541-6550.
28. Phillips HS, Kharbanda S, Chen R, et al. Molecular subclasses of highgradeglioma predict prognosis, delineate a pattern of disease progression,and resemble stages in neurogenesis. Cancer Cell.2006;9:157-173.
29. Rieske P, Golanska E, Zakrzewska M, et al. Arrested neural andadvanced mesenchymal differentiation of glioblastoma cellscomparativestudy with neural progenitors. BMC Cancer. 2009;9:54.
30. Tso CL, Shintaku P, Chen J, et al. Primary glioblastomas express mesenchymalstem-like properties. Mol Cancer Res. 2006;4:607-619.
31. Foroni C, Galli R, Cipelletti B, et al. Resilience to transformation andinherent genetic and functional stability of adult neural stem cells exvivo. Cancer Res. 2007;67:3725-3733.
32. Machon O, Backman M, Krauss S, Kozmik Z. The cellular fate of corticalprogenitors is not maintained in neurosphere cultures. Mol CellNeurosci. 2005;30:388-397.
33. Decarvalho AC, Nelson K, Lemke N, et al. Gliosarcoma stem cellsundergo glial and mesenchymal differentiation in vivo. Stem Cells.2010;28:181-190.
34. Rappa G, Mercapide J, Anzanello F, et al. Growth of cancer cell linesunder stem cell-like conditions has the potential to unveil therapeutictargets. Exp Cell Res. 2008;314:2110-2122.
35. Yu SC, Ping YF, Yi L, et al. Isolation and characterization of cancer stemcells from a human glioblastoma cell line U87. Cancer Lett. 2008;265:124-134.
36. Cancer Genome Atlas Research Network. Comprehensive genomiccharacterization defines human glioblastoma genes and core pathways.Nature. 2008;455:1061-1068.
37. Cahill DP, Levine KK, Betensky RA, et al. Loss of the mismatch repairprotein MSH6 in human glioblastomas is associated with tumorprogression during temozolomide treatment. Clin Cancer Res. 2007;13:2038-2045.