Neuronal calcium sensor synaptotagmin-9 is not involved in the regulation of glucose homeostasis or insulin secretion

PLoS ONE

Volumn 5, Issue 11, 2010, Pages

  Gustavsson, Natalia ^a   Wang, Xiaorui ^a   Wang, Yue ^a   Seah, Tingting ^a   Xu, Jun ^b   Radda, George K ^a   Südhof, Thomas C ^b   Han, Weiping ^a,c  

^a AGENCY FOR SCIENCE   (Singapore)

^b STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM ION; GLUCOSE; INSULIN; SYNAPTOTAGMIN; SYNAPTOTAGMIN 9; UNCLASSIFIED DRUG; CALCIUM;

ANIMAL TISSUE; ARTICLE; BODY WEIGHT; CALCIUM SIGNALING; CONTROLLED STUDY; EXOCYTOSIS; GENE DELETION; GLUCOSE BLOOD LEVEL; GLUCOSE HOMEOSTASIS; GLUCOSE TOLERANCE; IN VIVO STUDY; INSULIN BLOOD LEVEL; INSULIN RELEASE; ISOLATED PANCREAS ISLET; MOUSE; NONHUMAN; ANIMAL; CELL MEMBRANE; CELL MEMBRANE POTENTIAL; DRUG EFFECT; ELECTRIC CAPACITANCE; ELECTRON MICROSCOPY; FEMALE; GENETICS; GLUCOSE TOLERANCE TEST; HOMEOSTASIS; MALE; METABOLISM; MOUSE MUTANT; NERVE CELL; PANCREAS ISLET; PANCREAS ISLET BETA CELL; PATCH CLAMP; PHYSIOLOGY; SECRETION; ULTRASTRUCTURE;

MUS;

ANIMALS; BODY WEIGHT; CALCIUM; CELL MEMBRANE; ELECTRIC CAPACITANCE; EXOCYTOSIS; FEMALE; GLUCOSE; GLUCOSE TOLERANCE TEST; HOMEOSTASIS; INSULIN; INSULIN-SECRETING CELLS; ISLETS OF LANGERHANS; MALE; MEMBRANE POTENTIALS; MICE; MICE, KNOCKOUT; MICROSCOPY, ELECTRON; NEURONS; PATCH-CLAMP TECHNIQUES; SYNAPTOTAGMINS;

EID: 78649729986     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0015414     Document Type: Article

References (26)

1. Henquin JC, Nenquin M, Ravier MA, Szollosi A (2009) Shortcomings of current models of glucose-induced insulin secretion. Diabetes Obes Metab 11 (Suppl 4): 168-179.
2. Ashcroft FM, Proks P, Smith PA, Ammala C, Bokvist K, et al. (1994) Stimulus-secretion coupling in pancreatic beta cells. J Cell Biochem 55 Suppl:. pp 54-65.
3. Gauthier BR, Wollheim CB (2008) Synaptotagmins bind calcium to release insulin. Am J Physiol Endocrinol Metab 295: E1279-1286.
4. Gustavsson N, Han W (2009) Calcium-sensing beyond neurotransmitters: functions of synaptotagmins in neuroendocrine and endocrine secretion. Biosci Rep 29: 245-259.
5. Marshall C, Hitman GA, Partridge CJ, Clark A, Ma H, et al. (2005) Evidence that an isoform of calpain-10 is a regulator of exocytosis in pancreatic beta-cells. Mol Endocrinol 19: 213-224.
6. Südhof TC (2002) Synaptotagmins: why so many? J Biol Chem 277: 7629-7632.
7. Geppert M, Goda Y, Hammer RE, Li C, Rosahl TW, et al. (1994) Synaptotagmin I: a major Ca2+ sensor for transmitter release at a central synapse. Cell 79: 717-727.
8. Pang ZP, Melicoff E, Padgett D, Liu Y, Teich AF, et al. (2006) Synaptotagmin-2 is essential for survival and contributes to Ca2+ triggering of neurotransmitter release in central and neuromuscular synapses. J Neurosci 26: 13493-13504.
9. Xu J, Mashimo T, Südhof TC (2007) Synaptotagmin-1, -2, and -9: Ca(2+) sensors for fast release that specify distinct presynaptic properties in subsets of neurons. Neuron 54: 567-581.
10. Schonn JS, Maximov A, Lao Y, Südhof TC, Sorensen JB (2008) Synaptotagmin-1 and -7 are functionally overlapping Ca2+ sensors for exocytosis in adrenal chromaffin cells. Proc Natl Acad Sci U S A 105: 3998-4003.
11. Barg S, Rorsman P (2004) Insulin secretion: a high-affinity Ca2+ sensor after all? J Gen Physiol 124: 623-625.
12. Rettig J, Neher E (2002) Emerging roles of presynaptic proteins in Ca++-triggered exocytosis. Science 298: 781-785.
13. Jahn R, Scheller RH (2006) SNAREs-engines for membrane fusion. Nat Rev Mol Cell Biol 7: 631-643.
14. Gerber SH, Südhof TC (2002) Molecular determinants of regulated exocytosis. Diabetes 51 (Suppl 1): S3-11.
15. Gustavsson N, Lao Y, Maximov A, Chuang JC, Kostromina E, et al. (2008) Impaired insulin secretion and glucose intolerance in synaptotagmin-7 null mutant mice. Proc Natl Acad Sci U S A 105: 3992-3997.
16. Gustavsson N, Wei SH, Hoang DN, Lao Y, Zhang Q, et al. (2009) Synaptotagmin-7 is a principal Ca2+ sensor for Ca2+ -induced glucagon exocytosis in pancreas. J Physiol 587: 1169-1178.
17. Lernmark A (1974) The preparation of, and studies on, free cell suspensions from mouse pancreatic islets. Diabetologia 10: 431-438.
18. Gopel S, Zhang Q, Eliasson L, Ma XS, Galvanovskis J, et al. (2004) Capacitance measurements of exocytosis in mouse pancreatic alpha-, beta- and delta-cells within intact islets of Langerhans. J Physiol 556: 711-726.
19. Lindau M, Neher E (1988) Patch-clamp techniques for time-resolved capacitance measurements in single cells. Pflugers Arch 411: 137-146.
20. Iezzi M, Eliasson L, Fukuda M, Wollheim CB (2005) Adenovirus-mediated silencing of synaptotagmin 9 inhibits Ca2+-dependent insulin secretion in islets. FEBS Lett 579: 5241-5246.
21. Zawalich WS, Zawalich KC (1996) Species differences in the induction of time-dependent potentiation of insulin secretion. Endocrinology 137: 1664-1669.
22. Zawalich WS, Yamazaki H, Zawalich KC (2008) Biphasic insulin secretion from freshly isolated or cultured, perifused rodent islets: comparative studies with rats and mice. Metabolism 57: 30-39.
23. Svoboda P (2007) Off-targeting and other non-specific effects of RNAi experiments in mammalian cells. Curr Opin Mol Ther 9: 248-257.
24. Turner MD, Fulcher FK, Jones CV, Smith BT, Aganna E, et al. (2007) Calpain facilitates actin reorganization during glucose-stimulated insulin secretion. Biochem Biophys Res Commun 352: 650-655.
25. Turner MD (2007) Coordinated control of both insulin secretion and insulin action through calpain-10-mediated regulation of exocytosis? Mol Genet Metab 91: 305-307.
26. Ort T, Voronov S, Guo J, Zawalich K, Froehner SC, et al. (2001) Dephosphorylation of beta2-syntrophin and Ca2+/mu-calpain-mediated cleavage of ICA512 upon stimulation of insulin secretion. EMBO J 20: 4013-4023.