The in vitro effects of 2-methoxyestradiol-bis-sulphamate on cell numbers, membrane integrity and cell morphology, and the possible induction of apoptosis and autophagy in a non-tumorigenic breast epithelial cell line

Cellular and Molecular Biology Letters

Volumn 15, Issue 4, 2010, Pages 564-581

  Visagie, Michelle H ^a   Joubert, Annie M ^a  

^a UNIVERSITY OF PRETORIA   (South Africa)

Author keywords

2 methoxyestradiol bis sulphamate; Apoptosis; Autophagy

Indexed keywords

2 METHOXYESTRADIOL BIS SULPHAMATE; ANTINEOPLASTIC AGENT; FLUORESCEIN ISOTHIOCYANATE; LACTATE DEHYDROGENASE; UNCLASSIFIED DRUG;

APOPTOSIS; ARTICLE; AUTOPHAGY; BREAST EPITHELIUM; CELL COUNT; CELL STRUCTURE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG MECHANISM; FLUORESCENCE MICROSCOPY; HUMAN; HUMAN CELL; IN VITRO STUDY; MEMBRANE DAMAGE; METAPHASE; STRUCTURE ANALYSIS; TRANSMISSION ELECTRON MICROSCOPY;

EID: 78649717886     PISSN: 14258153     EISSN: 16891392     Source Type: Journal    
DOI: 10.2478/s11658-010-0030-4     Document Type: Article

References (30)

1. Dubey, R. K. and Jackson, E. K. Potential vascular actions of 2-methoxyestradiol. Trends Endocrinol. Metab. 20 (2009) 374-379.
2. Pribluda, V. S., Gubish, E. R., La Vallee, T. M., Treston, A., Swartz, G. M and Green, S. J. 2-Methylestradiol: An endogenous antiangiogenic and antiproliferative drug candidate. Cancer Metastasis Rev. 19 (2000) 173-179.
3. Bhati, R., Gokmen-Polar, Y., Sledge, G. W., Fan, C., Nakshatri, H., Ketelsen, D., Borchers, C. H., Dial, M. J., Patterson, C. and Klauber-DeMore, N. 2-Methoxyestradiol inhibits the anaphase-promoting complex and protein translation in human breast cancer cells. Cancer Res. 67 (2007) 702-708.
4. Banerjee, S. N., Sengupta, K., Banerjee, S., Saxene, N. K. and Banerjee, S. K. 2-Methoxyestradiol exhibits a biphasic effect on VEGF-A in tumor cells and upregulation is mediated through ER-α: a possible signaling pathway associated with the impact of 2-ME2 on proliferating cells. Neoplasia5 (2003) 417-426.
5. Funakoshi, T., Birsner, A. E. and D'Amato, R. J. Antiangiogenic effect of oral 2-methoxyestradiol on choroidal neovascularization in mice. Exp. Eye Res. 83 (2006) 1102-1107.
6. Matei, D., Schilder, J., Sutton, G., Perkins, S., Breen, T., Quon, C. and Sidor, C. Activity of 2 methoxyestradiol (Panzem® NCD) in advanced, platinum-resistant ovarian cancer and primary peritoneal carcinomatosis: A hoosier oncology group trial. Gynaecol. Oncol. 115 (2009) 90-96.
7. Lakka, S. S. and Rao, J. S. Angiogenic therapy in brain tumors. Expert Rev. Neurother. 8 (2008) 1457-1473.
8. Sutherland, T. E., Anderson, R. L., Hughes, R. A., Altmann, E., Schuliga, M., Ziogas, J. and Stewart, A. G. 2-Methoxyestradiol- a unique blend of activities generating a new class of anti-tumour/anti-inflammatory agents. Drug Discov. Today12 (2007) 577-584.
9. Thaver, V., Lottering, M. L., van Papendorp, D. and Joubert, A. In vitro effects of 2-methoxyestradiol on cell numbers, morphology, cell cycle progression, and apoptosis induction in oesophageal carcinoma cells. Cell Biochem. Funct. 27 (2009) 205-210.
10. Lakhani, N. J., Lepper, E. R., Sparreboom, A., Dahut, W. L., Venitz, J. and Figg, W. D. Determination of 2-methxyestradiol in human plasma, using liquid chromatography/tandem mass spectrometry. Rapid Commun. Mass. Spectrom. 19 (2005) 1176-1182.
11. Foster, P. A., Ho, Y. T., Newman, S. P., Kasprzyk, M. P., Leese, M. P., Potter, B. V., Reed, M. J. and Purohit, A. 2-MeOE2bisMATE and 2-EtE2bisMATE induce cell cycle arrest and apoptosis in breast cancer xenografts as shown by a novel ex vivo technique. Breast Cancer Res. Treat. 111 (2008) 251-260.
12. Reed, M. J., Purohit, A., Woo, L. W. L., Newman, S. P. and Potter, B. V. L. Steroid sulfatase: molecular biology, regulation, and inhibition. Endocr. Rev. 26 (2005) 171-202.
13. Kirches, E. and Wraich-Kirches, M. 2-Methoxyestradiol as a potential cytostatic drug in gliomas? Anticancer Agents Med. Chem. 9 (2009) 55-65.
14. Utsumi, T., Leese, M. P., Chander, S. K., Gaukroger, K., Purohit, A., Newman, S. P., Potter B. V. and Reed, M. J. The effects of 2-methoxyoestrogen sulphamates on the in vitro and in vivo proliferation of breast cancer cells. J. Steroid Biochem. Mol. Biol. 94 (2005) 219-227.
15. Tagg, S. L., Foster, P. A., Leese, M. P., Potter, B. V., Reed, M. J., Purohit, A. and Newman, S. P. 2-methoxyoestradiol-3, 27-O, O-bis-sulphamate and 2-deoxy-D-glucose in combination: a potential treatment for breast and prostate cancer. Br. J. Cancer99 (2008) 1842-1848.
16. Ireson, C. R., Chander, S. K., Purohit, A., Perera, S., Newman, S. P., Parish, D., Leese, M. P., Smith, A. C., Potter, B. V. and Reed, M. J. Pharmacokinetics and efficacy of 2-methoxyoestradiol and 2-methoxyoestradiol-bis-sulphamate in vivo in rodents. Br. J. Cancer90 (2004) 932-937.
17. Suzuki, R. N., Newman, S. P., Purohit, A., Leese, M. P., Potter, B. V. and Reed, M. J. Growth inhibition of multi-drug-resistant breast cancer cells by 2-methoxyoestradiol-bis-sulphamate and 2-ethyloestradiol-bis-sulphamate. J. Steroid Biochem. Mol. Biol. 84 (2003) 269-278.
18. Foster, P. A., Newman, S. P., Leese, M. P., Bernetiere, S., Diolez, C., Camara, J., Hacher, B., Baronnet, M. M., Ali, T., Potter, B. V., Reed, M. J. and Purohit, A. A new micronized formulation of 2-methoxyestradiol-bis-sulphamate (STX140) is therapeutically potent against breast cancer. Anticancer Res. 28 (2008) 577-581.
19. Wood, L., Leese, M. P., Mouzakiti, A., Purohit, A., Potter, B. V., Reed, M. J. and Packham, G. 2-MeOE2bisMATE induces caspase-dependent apoptosis in CAL51 breast cancer cells and overcomes resistance to TRAIL via cooperative activation of caspases. Apoptosis9 (2004) 323-332.
20. Newman, S. P., Leese, M. P., Purohit, A., James, D. R. C., Rennie, C. E., Potter, B. V. and Reed, M. J. Inhibition of in vitro angiogenesis by 2-Methoxy- and 2-ethyl-estrogen sulphamates. Int. J. Cancer109 (2004) 533-540.
21. Joubert, A. M., Marais, S. and Maritz, C. Influence of 2-methoxyestradiol on MCF-7 cells: An improved differential interference contrasting technique and Bcl-2 and Bax protein expression levels. Biocell33 (2009) 67-70.
22. Gillies, R. J., Didier, N. and Denton, M. Determination of cell number in monolayer cultures. Anal. Biochem. 159 (1986) 109-113.
23. Grever, M. R., Schepartz, S. A. and Chabner, B. A. The National Cancer Institute: cancer drug discovery and development program. Semin. Oncol. 19 (1992) 622-638.
24. Kusuzaki, K., Murata, H., Takeshita, H., Hashiguchi, S., Nozaki, T., Emoto, K., Ashihara, T. and Hirasawa, Y. Intracellular binding sites of acridine orange in living osteosarcoma cells. Anticancer Res. 20 (2000) 971-975.
25. Elmore, S. Apoptosis: a Review of programmed cell death. Toxicol. Pathol. 35 (2007) 495-516.
26. Xia, GH., Chen, B. A., Shao, Z. Y., Lu, H. X., Konstanze, D. and Hartmut, D. Mechanism of 2-methoxyestradiol-induced apoptosis in myelodysplastic syndrome MUTZ-1 cell line. Zhongguo Shi Yan Xue Ye Xue Za Zhi15 (2007) 296-301.
27. Kachadourian, R., Liochev, S. I., Cabelli, D. E., Patel, M. N., Fridovich, I. and Day, B. J. 2-Methoxyestradiol does not inhibit superoxide dismutase. Arch. Biochem. Biophys. 392 (2001) 349-353.
28. Fu, J., Weise, A. M., Falany, J. L., Falany, C. N., Thibodeau, B. J., Miller, F. R., Korarek, T. A. and Runge-Morris, M. Expression of estrogenicity genes in a lineage cell culture model of human breast cancer progression. Breast Cancer Res. Treat. 120 (2010) 35-40.
29. Yoshimura, N., Harada, N., Bukholm, I., Karesen, R., Borresen-Dale, A. and Kristensen, V. N. Intratumoural mRNA expression of genes from the oestradiol metabolic pathway and clinical and histopathological parameters of breast cancer. Breast Cancer Res. 6 (2004) R46-R55.
30. Stanway, S. J., Delavault, P., Purohit, A., Woo, L. W., Thurieau, C., Potter, B. V. and Reed, M. J. Steroid sulfatase: a new target for the endocrine therapy of breast cancer. Oncologist12 (2007) 370-374.