Ethidium bromide MIC screening for enhanced efflux pump gene expression or efflux activity in Staphylococcus aureus

Antimicrobial Agents and Chemotherapy

Volumn 54, Issue 12, 2010, Pages 5070-5073

  Patel, Diixa ^a   Kosmidis, Christos ^a   Seo, Susan M ^a   Kaatz, Glenn W ^a,b  

^a WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b VETERANS AFFAIRS MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ETHIDIUM BROMIDE;

ARTICLE; BACTERIAL GENE; GENE EXPRESSION; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; MINIMUM INHIBITORY CONCENTRATION; MULTIDRUG RESISTANCE; NONHUMAN; NORA GENE; NORB GENE; NORC GENE; PRIORITY JOURNAL; QACA GENE; QACB GENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STAPHYLOCOCCUS AUREUS;

ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; DRUG RESISTANCE, MULTIPLE, BACTERIAL; ETHIDIUM; GENE EXPRESSION REGULATION, BACTERIAL; HUMANS; MICROBIAL SENSITIVITY TESTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; STAPHYLOCOCCUS AUREUS;

EID: 78649643920     PISSN: 00664804     EISSN: 10986596     Source Type: Journal    
DOI: 10.1128/AAC.01058-10     Document Type: Article

References (22)

1. CLSI. 2006. Approved standard M7-A7. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. 7th ed. Clinical and Laboratory Standards Institute, Wayne, PA.
2. DeMarco, C. E., L. A. Cushing, E. Frempong-Manso, S. M. Seo, T. A. Jaravaza, and G. W. Kaatz. 2007. Efflux-related resistance to norfloxacin, dyes, and biocides in bloodstream isolates of Staphylococcus aureus. Antimicrob. Agents Chemother. 51:3235-3239.
3. Frempong-Manso, E., J. L. Raygada, C. E. DeMarco, S. M. Seo, and G. W. Kaatz. 2009. Inability of a reserpine-based screen to identify strains overexpressing efflux pump genes in clinical isolates of Staphylococcus aureus. Int. J. Antimicrob. Agents 33:360-363.
4. Grkovic, S., K. M. Hardie, M. H. Brown, and R. A. Skurray. 2003. Interactions of the QacR multidrug-binding protein with structurally diverse ligands: implications for the evolution of the binding pocket. Biochemistry 42:15226-15236.
5. Hassan, K. A., R. A. Skurray, and M. H. Brown. 2007. Active export proteins mediating drug resistance in staphylococci. J. Mol. Microbiol. Biotechnol. 12:180-196.
6. Horsburgh, M. J., J. L. Aish, I. J. White, L. Shaw, J. K. Lithgow, and S. J. Foster. 2002. sB modulates virulence determinant expression and stress resistance: characterization of a functional rsbU strain derived from Staphylococcus aureus 8325-4. J. Bacteriol. 184:5457-5467.
7. Huang, J., P. W. O'Toole, W. Shen, H. Amrine-Madsen, X. Jiang, N. Lobo, L. M. Palmer, L. Voelker, F. Fan, M. N. Gwynn, and D. McDevitt. 2004. Novel chromosomally encoded multidrug efflux transporter MdeA in Staphylococcus aureus. Antimicrob. Agents Chemother. 48:909-917.
8. Huet, A. A., J. L. Raygada, K. Mendiratta, S. M. Seo, and G. W. Kaatz. 2008. Multidrug efflux pump overexpression in Staphylococcus aureus after single and multiple in vitro exposures to biocides and dyes. Microbiology 154:3144-3153.
9. Jarvis, W. R., J. Schlosser, R. Y. Chinn, S. Tweeten, and M. Jackson. 2007. National prevalence of methicillin-resistant Staphylococcus aureus in inpatients at US health care facilities, 2006. Am. J. Infect. Control 35:631-637.
10. Kaatz, G. W., C. E. DeMarco, and S. M. Seo. 2006. MepR, a repressor of the Staphylococcus aureus MATE family multidrug efflux pump MepA, is a substrate-responsive regulatory protein. Antimicrob. Agents Chemother. 50:1276-1281.
11. Kaatz, G. W., F. McAleese, and S. M. Seo. 2005. Multidrug resistance in Staphylococcus aureus due to overexpression of a novel multidrug and toxin extrusion (MATE) transport protein. Antimicrob. Agents Chemother. 49:1857-1864.
12. Kaatz, G. W., S. M. Seo, L. O'Brien, M. Wahiduzzaman, and T. J. Foster. 2000. Evidence for the existence of a multidrug efflux transporter distinct from NorA in Staphylococcus aureus. Antimicrob. Agents Chemother. 44:1404-1406.
13. Kaatz, G. W., S. M. Seo, and C. A. Ruble. 1993. Efflux-mediated fluoroquinolone resistance in Staphylococcus aureus. Antimicrob. Agents Chemother. 37:1086-1094.
14. Markham, P. N., and A. A. Neyfakh. 1996. Inhibition of the multidrug transporter NorA prevents emergence of norfloxacin resistance in Staphylococcus aureus. Antimicrob. Agents Chemother. 40:2673-2674.
15. Martins, M., I. Couto, M. Viveiros, and L. Amaral. 2010. Identification of efflux-mediated multi-drug resistance in bacterial clinical isolates by two simple methods. Methods Mol. Biol. 642:143-157.
16. Narui, K., N. Noguchi, K. Wakasugi, and M. Sasatsu. 2002. Cloning and characterization of a novel chromosomal drug efflux gene in Staphylococcus aureus. Biol. Pharm. Bull. 25:1533-1536.
17. Paulsen, I. T., M. H. Brown, T. G. Littlejohn, B. A. Mitchell, and R. A. Skurray. 1996. Multidrug resistance proteins QacA and QacB from Staphylococcus aureus: membrane topology and identification of residues involved in substrate specificity. Proc. Natl. Acad. Sci. U. S. A. 93:3630-3635.
18. Poole, K. 2005. Efflux-mediated antimicrobial resistance. J. Antimicrob. Chemother. 56:20-51.
19. Resch, A., S. Leicht, M. Saric, L. Pasztor, A. Jakob, F. Gotz, and A. Nordheim. 2006. Comparative proteome analysis of Staphylococcus aureus biofilm and planktonic cells and correlation with transcriptome profiling. Proteomics 6:1867-1877.
20. Truong-Bolduc, Q. C., P. M. Dunman, J. Strahilevitz, S. J. Projan, and D. C. Hooper. 2005. MgrA is a multiple regulator of two new efflux pumps in Staphylococcus aureus. J. Bacteriol. 187:2395-2405.
21. Truong-Bolduc, Q. C., J. Strahilevitz, and D. C. Hooper. 2006. NorC, a new efflux pump regulated by MgrA of Staphylococcus aureus. Antimicrob. Agents Chemother. 50:1104-1107.
22. Yamada, Y., K. Hideka, S. Shiota, T. Kuroda, and T. Tsuchiya. 2006. Gene cloning and characterization of SdrM, a chromosomally-encoded multidrug efflux pump, from Staphylococcus aureus. Biol. Pharm. Bull. 29:554-556.