Proplatelet formation deficit and megakaryocyte death contribute to thrombocytopenia in Myh9 knockout mice

Journal of Thrombosis and Haemostasis

Volumn 8, Issue 10, 2010, Pages 2243-2251

  Eckly, A ^a   Rinckel, J Y ^a   Laeuffer, P ^a   Cazenave, J P ^a   Lanza, F ^a   Gachet, C ^a   Leon C ^a  

^a UNIVERSITÉ DE STRASBOURG   (France)

Author keywords

Bone marrow explant; Knockout mice; Megakaryocyte; MYH9 related diseases; Myosin IIA; Thrombopoiesis

Indexed keywords

BLEBBISTATIN; CASPASE 3; MYH PROTEIN; MYOSIN; PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBODY LABELING; APOPTOSIS; ARTICLE; BONE MARROW CELL; CELL DEATH; CELL MATURATION; CELL VIABILITY; COMPARATIVE STUDY; EXPLANT; KNOCKOUT MOUSE; MEGAKARYOCYTE; MOUSE; NICK END LABELING; NONHUMAN; PRIORITY JOURNAL; THROMBOCYTOPENIA; THROMBOCYTOPOIESIS; TRANSMISSION ELECTRON MICROSCOPY; WILD TYPE;

ANIMALS; BLOOD PLATELETS; BONE MARROW; CASPASE 3; CELL DEATH; CELL LINEAGE; CELL SURVIVAL; FEMALE; HETEROCYCLIC COMPOUNDS WITH 4 OR MORE RINGS; IN SITU NICK-END LABELING; MALE; MEGAKARYOCYTES; MICE; MICROSCOPY, ELECTRON, TRANSMISSION; MUTATION; NONMUSCLE MYOSIN TYPE IIA; THROMBOCYTOPENIA;

EID: 78649344756     PISSN: 15387933     EISSN: 15387836     Source Type: Journal    
DOI: 10.1111/j.1538-7836.2010.04009.x     Document Type: Article

References (30)

1. Althaus K, Greinacher A. MYH9-related platelet disorders. Semin Thromb Hemost 2009; 35: 189-203.
2. Balduini CL. Giant platelet syndromes and the MYH9 mutations. Lab Hematol 2004; 10: 187-8.
3. Seri M, Pecci A, Di Bari F, Cusano R, Savino M, Panza E, Nigro A, Noris P, Gangarossa S, Rocca B, Gresele P, Bizzaro N, Malatesta P, Koivisto PA, Longo I, Musso R, Pecoraro C, Iolascon A, Magrini U, Rodriguez Soriano J, et al. MYH9-related disease: May-Hegglin anomaly, Sebastian syndrome, Fechtner syndrome, and Epstein syndrome are not distinct entities but represent a variable expression of a single illness. Medicine (Baltimore) 2003; 82: 203-15.
4. Eckly A, Strassel C, Freund M, Cazenave JP, Lanza F, Gachet C, Leon C. Abnormal megakaryocyte morphology and proplatelet formation in mice with megakaryocyte-restricted MYH9 inactivation. Blood 2009; 113: 3182-9.
5. Chang Y, Aurade F, Larbret F, Zhang Y, Le Couedic JP, Momeux L, Larghero J, Bertoglio J, Louache F, Cramer E, VainchenkerW, Debili N. Proplatelet formation is regulated by the Rho/ROCK pathway. Blood 2007; 109: 4229-36.
6. Chen Z, Naveiras O, Balduini A, Mammoto A, ContiMA, Adelstein RS, Ingber D, Daley GQ, Shivdasani RA. TheMay-Hegglin anomaly gene MYH9 is a negative regulator of platelet biogenesis modulated by the Rho-ROCK pathway. Blood 2007; 110: 171-9.
7. Chang Y, Bluteau D, Debili N, Vainchenker W. From hematopoietic stem cells to platelets. J Thromb Haemost 2007; 5(Suppl 1): 318-27.
8. Chen Z, Shivdasani RA. Regulation of platelet biogenesis: insights from the May-Hegglin anomaly and other MYH9-related disorders. J Thromb Haemost 2009; 7(Suppl 1): 272-6.
9. Thiery JP, Bessis M. Genesis of blood platelets from the megakaryocytes in living cells. C R Hebd Seances Acad Sci 1956; 242: 290-2.
10. Thiery JP, Bessis M. [Mechanism of platelet genesis; in vitro study by cinemicrophotography.]. Rev Hematol 1956; 11: 162-74.
11. Perrault C, Moog S, Rubinstein E, Santer M, Baas MJ, de la Salle C, Ravanat C, Dambach J, Freund M, Santoso S, Cazenave JP, Lanza F. A novel monoclonal antibody against the extracellular domain of GPIbbeta modulates vWF mediated platelet adhesion. Thromb Haemost 2001; 86: 1238-48.
12. Leon C, Eckly A, Hechler B, Aleil B, FreundM, Ravanat C, Jourdain M, Nonne C, Weber J, Tiedt R, Gratacap MP, Severin S, Cazenave JP, Lanza F, Skoda R, Gachet C. Megakaryocyte-restricted MYH9 inactivation dramatically affects hemostasis while preserving platelet aggregation and secretion. Blood 2007; 110: 3183-91.
13. Askenasy N, Yolcu ES, Shirwan H, Stein J, Yaniv I, Farkas DL. Characterization of adhesion and viability of early seeding hematopoietic cells in the host bone marrow in vivo and in situ. Exp Hematol 2003; 31: 1292-300.
14. Edinger AL, Thompson CB. Death by design: apoptosis, necrosis and autophagy. Curr Opin Cell Biol 2004; 16: 663-9.
15. Clarke MC, Savill J, Jones DB, Noble BS, Brown SB. Compartmentalized megakaryocyte death generates functional platelets committed to caspase-independent death. J Cell Biol 2003; 160: 577-87.
16. De Botton S, Sabri S, Daugas E, Zermati Y, Guidotti JE, Hermine O, Kroemer G, Vainchenker W, Debili N. Platelet formation is the consequence of caspase activation within megakaryocytes. Blood 2002; 100: 1310-7.
17. Avecilla ST, Hattori K, Heissig B, Tejada R, Liao F, Shido K, JinDK, Dias S, Zhang F, Hartman TE, Hackett NR, Crystal RG, Witte L, Hicklin DJ, Bohlen P, Eaton D, Lyden D, de Sauvage F, Rafii S. Chemokine-mediated interaction of hematopoietic progenitors with the bone marrow vascular niche is required for thrombopoiesis. Nat Med 2004; 10: 64-71.
18. Wolfenson H, Henis YI, Geiger B, Bershadsky AD. The heel and toe of the cell's foot: a multifaceted approach for understanding the structure and dynamics of focal adhesions. Cell Motil Cytoskeleton 2009; 66: 1017-29.
19. Schulze H, Korpal M, Hurov J, Kim SW, Zhang J, Cantley LC, Graf T, Shivdasani RA. Characterization of the megakaryocyte demarcation membrane system and its role in thrombopoiesis. Blood 2006; 107: 3868-75.
20. Raslova H, Baccini V, Loussaief L, Comba B, Larghero J, Debili N, Vainchenker W. Mammalian target of rapamycin (mTOR) regulates both proliferation of megakaryocyte progenitors and late stages of megakaryocyte differentiation. Blood 2006; 107: 2303-10.
21. Heynen MJ, Blockmans D, Verwilghen RL, Vermylen J. Congenital macrothrombocytopenia, leucocyte inclusions, deafness and proteinuria: functional and electron microscopic observations on platelets and megakaryocytes. Br J Haematol 1988; 70: 441-8.
22. Parsa KP, Lee DB, Zamboni L, Glassock RJ. Hereditary nephritis, deafness and abnormal thrombopoiesis. Study of a new kindred. Am J Med 1976; 60: 665-72.
23. Zucker-Franklin D. Megakaryocytes and platelets. In: Zucker-Franklin D, Grossi CE, eds. Atlas of blood cells. Milan: Edi. Ermes, 2003: 788-91.
24. Pecci A, Malara A, Badalucco S, Bozzi V, Torti M, Balduini CL, Balduini A. Megakaryocytes of patients with MYH9-related thrombocytopenia present an altered proplatelet formation. Thromb Haemost 2009; 102: 90-6.
25. Houwerzijl EJ, Blom NR, van der Want JJ, Esselink MT, Koornstra JJ, Smit JW, Louwes H, Vellenga E, deWolf JT. Ultrastructural study shows morphologic features of apoptosis and para-apoptosis in megakaryocytes from patients with idiopathic thrombocytopenic purpura. Blood 2004; 103: 500-6.
26. Houwerzijl EJ, Blom NR, van der Want JJ, Louwes H, Esselink MT, Smit JW, Vellenga E, de Wolf JT. Increased peripheral platelet destruction and caspase-3-independent programmed cell death of bone marrow megakaryocytes in myelodysplastic patients. Blood 2005; 105: 3472-9.
27. Houwerzijl EJ, Blom NR, van der Want JJ, Vellenga E, de Wolf JT. Megakaryocytic dysfunction in myelodysplastic syndromes and idiopathic thrombocytopenic purpura is in part due to different forms of cell death. Leukemia 2006; 20: 1937-42.
28. Laevsky G, Knecht DA. Cross-linking of actin filaments by myosin II is amajor contributor to cortical integrity and cellmotility in restrictive environments. J Cell Sci 2003; 116: 3761-70.
29. Pasternak C, Spudich JA, Elson EL. Capping of surface receptors and concomitant cortical tension are generated by conventional myosin. Nature 1989; 341: 549-51.
30. Cai Y, RossierO, Gauthier NC, Biais N, Fardin MA, Zhang X, Miller LW, Ladoux B, Cornish VW, Sheetz MP. Cytoskeletal coherence requires myosin-IIA contractility. J Cell Sci 2010; 123: 413-23.