Structured coculture of stem cells and disc cells prevent disc degeneration in a rat model

Spine Journal

Volumn 10, Issue 12, 2010, Pages 1089-1097

  Allon, Aliza A ^a   Aurouer, Nicolas ^a,b   Yoo, Bryan B ^a   Liebenberg, Ellen C ^a   Buser, Zorica ^a   Lotz, Jeffrey C ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b BORDEAUX UNIVERSITY HOSPITAL   (France)

Author keywords

Bilaminar cell pellet; Coculture; Disc regeneration; Mesenchymal stem cell; Rat tail model

Indexed keywords

CYTOKINE; FIBRIN GLUE; PROTEOGLYCAN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL DIFFERENTIATION; CELL STRUCTURE; CELL THERAPY; COCULTURE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; EXTRACELLULAR MATRIX; HISTOLOGY; IN VITRO STUDY; IN VIVO STUDY; INTERVERTEBRAL DISK DEGENERATION; MALE; NONHUMAN; NUCLEUS PULPOSUS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RAT; SCORING SYSTEM; STAINING; STEM CELL;

ANIMALS; CELLS, CULTURED; COCULTURE TECHNIQUES; CYTOKINES; ENZYME-LINKED IMMUNOSORBENT ASSAY; FIBRIN TISSUE ADHESIVE; GUIDED TISSUE REGENERATION; INFLAMMATION; INTERVERTEBRAL DISK; INTERVERTEBRAL DISK DEGENERATION; MALE; MESENCHYMAL STEM CELL TRANSPLANTATION; MESENCHYMAL STEM CELLS; PROTEOGLYCANS; RATS; RATS, SPRAGUE-DAWLEY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 78649319725     PISSN: 15299430     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.spinee.2010.09.014     Document Type: Article

References (52)

1. J.U. Yoo, T.S. Barthels, and K. Nishimura The chondrogenic potential of human bone marrow derived mesenchymal progenitor cells J Bone Joint Surg Am 80 1998 1745 1757
2. A.A. Allon, R.A. Schneider, and J.C. Lotz Coculture of adult mesenchymal stem cells and nucleus pulposus cells in bilaminar pellets for intervertebral dics regeneration SAS J 3 2009 41 49
3. K. Singh, K. Masuda, and H.S. An Animal models for human disc degeneration Spine J 5 6 Suppl 2005 267S 279S
4. H.S. An, and K. Masuda Relevance of in vitro and in vivo models for intervertebral disc degeneration J Bone Joint Surg Am 88 Suppl 2 2006 88 94
5. J.C. Lotz Animal models of intervertebral disc degeneration: lessons learned Spine 29 2004 2742 2750
6. J.H. Schimandle, and S.D. Boden Spine update. Animal use in spinal research Spine 19 1994 2474 2477
7. D.M. Elliott, and J.J. Sarver Young investigator award winner: validation of the mouse and rat disc as mechanical models of the human lumbar disc Spine 29 2004 713 722
8. C.J. Hunter, J.R. Matyas, and N.A. Duncan Cytomorphology of notochordal and chondrocytic cells from the nucleus pulposus: a species comparison J Anat 205 2004 357 362
9. Y. Aoki, S. Ohtori, and H. Ino Disc inflammation potentially promotes axonal regeneration of dorsal root ganglion neurons innervating lumbar intervertebral disc in rats Spine 29 2004 2621 2626 (Pubitemid 40353247)
10. J.A. Ulrich, E.C. Liebenberg, D.U. Thuillier, and J.C. Lotz ISSLS prize winner: repeated disc injury causes persistent inflammation Spine 32 2007 2812 2819
11. M.A. Rousseau, J.A. Ulrich, and E.C. Bass Stab incision for inducing intervertebral disc degeneration in the rat Spine 32 2007 17 24
12. S.J. Lipson, and H. Muir 1980 Volvo award in basic science. Proteoglycans in experimental intervertebral disc degeneration Spine 6 1981 194 210
13. H. Takaishi, O. Nemoto, and M. Shiota Type-II collagen gene expression is transiently upregulated in experimentally induced degeneration of rabbit intervertebral disc J Orthop Res 15 1997 528 538
14. T. Nomura, J. Mochida, and M. Okuma Nucleus pulposus allograft retards intervertebral disc degeneration Clin Orthop Relat Res 389 2001 94 101
15. D.G. Anderson, M.W. Izzo, and D.J. Hall Comparative gene expression profiling of normal and degenerative discs: analysis of a rabbit annular laceration model Spine 27 2002 1291 1296
16. K. Masuda, Y. Aota, and C. Muehleman A novel rabbit model of mild, reproducible disc degeneration by an anulus needle puncture: correlation between the degree of disc injury and radiological and histological appearances of disc degeneration Spine 30 2005 5 14
17. D. Hampton, G. Laros, R. McCarron, and D. Franks Healing potential of the anulus fibrosus Spine 14 1989 398 401
18. O.L. Osti, B. Vernon-Roberts, and R.D. Fraser 1990 Volvo Award in experimental studies. Anulus tears and intervertebral disc degeneration. An experimental study using an animal model Spine 15 1990 762 767
19. R.J. Moore, B. Vernon-Roberts, O.L. Osti, and R.D. Fraser Remodeling of vertebral bone after outer anular injury in sheep Spine 21 1996 936 940
20. D.B. Ethier, J.E. Cain, and M.J. Yaszemski The influence of anulotomy selection on disc competence. A radiographic, biomechanical, and histologic analysis Spine 19 1994 2071 2076
21. E. Kaapa, S. Holm, and X. Han Collagens in the injured porcine intervertebral disc J Orthop Res 12 1994 93 102
22. A. Kanerva, B. Kommonen, and M. Gronblad Inflammatory cells in experimental intervertebral disc injury Spine 22 1997 2711 2715
23. S. Sobajima, J.F. Kompel, and J.S. Kim A slowly progressive and reproducible animal model of intervertebral disc degeneration characterized by MRI, X-ray, and histology Spine 30 2005 15 24
24. N. Boos, S. Weissbach, and H. Rohrbach Classification of age-related changes in lumbar intervertebral discs: 2002 Volvo Award in basic science Spine 27 2002 2631 2644
25. S. Sobajima, A.L. Shimer, and R.C. Chadderdon Quantitative analysis of gene expression in a rabbit model of intervertebral disc degeneration by real-time polymerase chain reaction Spine J 5 2005 14 23
26. K. Hasegawa, C.H. Turner, J. Chen, and D.B. Burr Effect of disc lesion on microdamage accumulation in lumbar vertebrae under cyclic compression loading Clin Orthop Relat Res 311 1995 190 198
27. K. Nishimura, and J. Mochida Percutaneous reinsertion of the nucleus pulposus. An experimental study Spine 23 1998 1531 1538 discussion 1539
28. A. Hiyama, J. Mochida, and J. Iwashina Transplantation of mesenchymal stem cells in a canine disc degeneration model J Orthop Res 26 2008 589 600
29. S. Junger, B. Gantenbein-Ritter, and P. Lezuo Effect of limited nutrition on in situ intervertebral disc cells under simulated-physiological loading Spine 34 2009 1264 1271
30. J.C. Lotz, A. Staples, A. Walsh, and A.H. Hsieh Mechanobiology in intervertebral disc degeneration and regeneration Conf Proc IEEE Eng Med Biol Soc 7 2004 5459
31. J.C. Lotz, and J.A. Ulrich Innervation, inflammation, and hypermobility may characterize pathologic disc degeneration: review of animal model data J Bone Joint Surg Am 88 Suppl 2 2006 76 82
32. H. Sheikh, K. Zakharian, and R.P. De La Torre In vivo intervertebral disc regeneration using stem cell-derived chondroprogenitors J Neurosurg Spine 10 2009 265 272
33. A. Hiyama, J. Mochida, and D. Sakai Stem cell applications in intervertebral disc repair Cell Mol Biol 54 2008 24 32
34. K.J. Saldanha, S.L. Piper, and K.M. Ainslie Magnetic resonance imaging of iron oxide labeled stem cells: application to tissue engineering based regeneration of the intervertebral disc Eur Cell Mater 16 2008 17 25
35. D. Sakai, J. Mochida, and T. Iwashina Differentiation of mesenchymal stem cells transplanted to a rabbit degenerative disc model: potential and limitations for stem cell therapy in disc regeneration Spine 30 2005 2379 2387
36. M.V. Risbud, I.M. Shapiro, A.R. Vaccaro, and T.J. Albert Stem cell regeneration of the nucleus pulposus Spine J 4 6 Suppl 2004 348S 353S
37. S.R. Bibby, D.A. Jones, R.M. Ripley, and J.P. Urban Metabolism of the intervertebral disc: effects of low levels of oxygen, glucose, and pH on rates of energy metabolism of bovine nucleus pulposus cells Spine 30 2005 487 496
38. H.A. Horner, and J.P. Urban Effect of nutrient supply on the viability of cells from the nucleus pulposus of the intervertebral disc Spine 26 2001 2543 2549
39. J.C. Lotz, and J.R. Chin Intervertebral disc cell death is dependent on the magnitude and duration of spinal loading Spine 25 2000 1477 1483
40. H.E. Gruber, J.A. Ingram, D.E. Davis, and E.N. Hanley Jr. Increased cell senescence is associated with decreased cell proliferation in vivo in the degenerating human annulus Spine J 9 2009 210 215
41. M. Gorensek, C. Jaksimovic, and N. Kregar-Velikonja Nucleus pulposus repair with cultured autologous elastic cartilage derived chondrocytes Cell Mol Biol Lett 9 2004 363 373
42. A.I. Chou, S.O. Akintoye, and S.B. Nicoll Photo-crosslinked alginate hydrogels support enhanced matrix accumulation by nucleus pulposus cells in vivo Osteoarthritis Cartilage 17 2009 1377 1384
43. L.J. Nesti, W.J. Li, and R.M. Shanti Intervertebral disc tissue engineering using a novel hyaluronic acid-nonfibrous scaffold amalgam Tissue Eng Part A 14 2008 1527 1537
44. X. Yang, and X. Li Nucleus pulposus tissue engineering: a brief review Eur Spine J 18 2009 1564 1572
45. A.A. Allon, R.A. Schneider, and J.C. Lotz Structured co-culture outperforms stem cells and disc cells in a simulated degenerative disc environment Trans Orthop Res Soc 35 2010 1456
46. J.H. Jeong, E.S. Jin, and J.K. Min Human mesenchymal stem cells implantation into the degenerated coccygeal disc of the rat Cytotechnology 59 2009 55 64
47. W.H. Chen, H.Y. Liu, and W.C. Lo Intervertebral disc regeneration in an ex vivo culture system using mesenchymal stem cells and platelet-rich plasma Biomaterials 30 2009 5523 5533
48. D. Sakai, J. Mochida, and T. Iwashina Regenerative effects of transplanting mesenchymal stem cells embedded in atelocollagen to the degenerated intervertebral disc Biomaterials 27 2006 335 345
49. G. Crevensten, A.J. Walsh, and D. Ananthakrishnana Intervertebral disc cell therapy for regeneration: mesenchymal stem cell implantation in rat intervertebral discs Ann Biomed Eng 32 2004 430 434
50. S. Sobajima, G. Vadala, and A. Shimer Feasability of a stem cell therapy for intervertebral disc degeneration Spine J 8 2008 888 896
51. E. Potier, E. Ferreira, and A. Meunier Prolonged hypoxia concomitant with serum deprivation induces massive human mesenchymal stem cell death Tissue Eng 13 2007 1325 1331
52. S.B. Early, K. Hise, and J.K. Han Hypoxia stimulates inflammatory and fibrotic responses from nasal-polyp derived fibroblasts Laryngoscope 117 2007 511 515