Experimental validation of the predicted binding site of Escherichia coli K1 outer membrane protein A to human brain microvascular endothelial cells: Identification of critical mutations that prevent E. coli meningitis

Journal of Biological Chemistry

Volumn 285, Issue 48, 2010, Pages 37753-37761

  Pascal, Tod A ^a   Abrol, Ravinder ^a   Mittal, Rahul ^b   Wang, Ying ^b   Prasadarao, Nemani V ^b,c   Goddard III, William A ^a  

^a CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

^b CHILDREN S HOSPITAL LOS ANGELES   (United States)

^c UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHITOBIOSE; E. COLI; EXPERIMENTAL VALIDATIONS; EXTRACELLULAR LOOPS; HUMAN BRAIN; MD SIMULATION; OUTER MEMBRANE PROTEIN A; THERAPEUTIC STRATEGY;

AMINO ACIDS; BINDING ENERGY; BIOLOGICAL MEMBRANES; BLOOD VESSELS; BRAIN; CARBOHYDRATES; ENDOTHELIAL CELLS; MOLECULAR DYNAMICS; SELF ASSEMBLY;

ESCHERICHIA COLI;

ALANINE; CHITOBIOSE; GLYCOPROTEIN GP 96; OUTER MEMBRANE PROTEIN A;

ARTICLE; BACTERIAL MENINGITIS; BACTERIAL STRAIN; BINDING SITE; BRAIN CAPILLARY; CHILD; COMPLEX FORMATION; CONTROLLED STUDY; ENDOTHELIUM CELL; ESCHERICHIA COLI; HUMAN; HUMAN CELL; MOLECULAR DYNAMICS; MOLECULAR MODEL; MUTATIONAL ANALYSIS; NEUROPATHOLOGY; NONHUMAN; PREDICTION; PRESCHOOL CHILD; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; SCHOOL CHILD; STRUCTURE ANALYSIS; THERMODYNAMICS; VALIDATION STUDY;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; BACTERIAL OUTER MEMBRANE PROTEINS; BINDING SITES; BRAIN; CELLS, CULTURED; CHILD; CHILD, PRESCHOOL; ENDOTHELIAL CELLS; ESCHERICHIA COLI; FEMALE; HUMANS; MALE; MENINGITIS; MOLECULAR CONFORMATION; MOLECULAR SEQUENCE DATA; MUTATION; NERVE TISSUE PROTEINS; PROTEIN BINDING; RECEPTORS, CELL SURFACE; VIRULENCE;

ESCHERICHIA COLI;

EID: 78549240628     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M110.122804     Document Type: Article

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