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Wrana, J.L.8
Dennis, J.W.9
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77951971475
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5 cells were re-suspended in test tubes and incubated in DPBS/1% BSA with 200 μl 2 μg/mL of the plant lectins L-PHA-FITC for 1 h at 4 °C. Cells were washed twice with DPBS, and fluorescence histogram profiles were determined using flow cytometric FACS analysis. Each compound was tested in triplicate and the experiments were repeated three times.
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Zhao, Y.1
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Boylek., M.O.'.3
Murphy, P.V.4
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8
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78449311623
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note
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3 cells) were seeded on 96-well microtitre plates in F12K medium with 10% FBS and incubated overnight. The cell culture medium was replaced by the different dose of a solution of 1 and the other compounds, and then the cells were cultured for another 72 h. The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] reagent was added to the cell supernatant for a final concentration of 0.5 mg/ml of MTT. After 3 h the cell culture medium was removed. Formazan crystals in adherent cells were dissolved in 200 μl DMSO and the absorbance of the formazan solution was measured. Each compound was tested in triplicate and the experiments were repeated three times.
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9
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78449272402
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note
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See Ref. 6. Cells were seeded on 6 well plates in F12K medium with 10% FBS overnight. Then 1 was added to the cells, which were then cultured for another 24 h. Cells were collected with trypsin-EDTA and washed three times with PBS. The cells were re-suspended and fixed for at least 2 h at -20 °C with 70% ethanol. After washing twice with PBS, cells were incubated at 25 °C with 200 μg/ml RNase A for 30 min. The resulting cells were incubated with 50 μg/ml propidium iodide for 30 min at 4 °C. The treated cells were subjected to flow cytometry and the percentage of cells at each phase of the cell cycle was analysed. Compound 1 was tested in triplicate and the experiment was repeated three times.
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10
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78449286746
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Jun 30. [Epub ahead of print] Briefly, after treatment with 1 (0, 50 and 100 μM for 24, 48 and 72 h), A549 cells were collected and washed with DPBS twice, and subjected to annexin V and propidium iodide staining using Annexin-V FITC apoptosis kit following the step-by-step protocol provided by the manufacturer (Biosource). After staining, flow cytometry was performed for the quantification of apoptotic cells. Compound 1 was tested in triplicate and the experiment was repeated three times in each case
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Zhao, Y.; Yang, G.; Ren, D.; Zhang, X.; Yin, Q.; Sun, X. Mol. Biol. Rep. 2010 Jun 30. [Epub ahead of print] Briefly, after treatment with 1 (0, 50 and 100 μM for 24, 48 and 72 h), A549 cells were collected and washed with DPBS twice, and subjected to annexin V and propidium iodide staining using Annexin-V FITC apoptosis kit following the step-by-step protocol provided by the manufacturer (Biosource). After staining, flow cytometry was performed for the quantification of apoptotic cells. Compound 1 was tested in triplicate and the experiment was repeated three times in each case.
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(2010)
Mol. Biol. Rep.
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Zhao, Y.1
Yang, G.2
Ren, D.3
Zhang, X.4
Yin, Q.5
Sun, X.6
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14
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78449290683
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note
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4 cells) were seeded on 96-well microtitre plates in F12K with 10% FBS overnight. The confluent monolayers were wounded with a 200 μl plastic pipette tip. After washing, the chamber slide was incubated at 37 °C in 10% FBS F12K media in the presence or absence of compounds. Migration of cells into wounded areas were photographed at 0 and 24 h.
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15
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23744513065
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D. Palm, K. Lang, B. Brandt, KS. Zaenker, and F. Entschladen Semin. Cancer Biol. 15 2005 396
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Palm, D.1
Lang, K.2
Brandt, B.3
Zaenker, K.S.4
Entschladen, F.5
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17
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78449312596
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note
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See Ref. 9. Cells were seeded to the glass coverslips in cell culture dishes (3.5 cm) overnight. Compound 1 was then added and cells were cultured for 24 h. After fixing with 4% paraformaldehyde, cells were treated with 0.1% Triton X-100 and blocked with 1% BSA. Cells were incubated with TRITC-conjugated phalloidin for 60 min and examined under Confocal Laser Scanning Microscope (100×, oil). The experiments were repeated at three times in each case.
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