Expression and purification of antimicrobial peptide CM4 by Npro fusion technology in E. coli

Amino Acids

Volumn 39, Issue 5, 2010, Pages 1545-1552

  Cheng, Xiawei ^a,b   Lu, Wuguang ^b   Zhang, Shuangquan ^a   Cao, Peng ^a,b  

^a NANJING NORMAL UNIVERSITY   (China)

^b Jiangsu Province Institute of Traditional Chinese Medicine   (China)

Author keywords

CM4; EDDIE; Escherichia coli; Optimization; Refolding

Indexed keywords

ANTIMICROBIAL PEPTIDE CM4; HYBRID PROTEIN; NICKEL; POLYPEPTIDE ANTIBIOTIC AGENT; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANTIMICROBIAL ACTIVITY; ANTINEOPLASTIC ACTIVITY; ARTICLE; CARBOXY TERMINAL SEQUENCE; CHELATION; CHEMOTROPH; CHROMATOGRAPHY; DRUG DETERMINATION; DRUG STRUCTURE; ESCHERICHIA COLI; GENE SWITCHING; IN VITRO STUDY; MASS SPECTROMETRY; MOLECULAR CLONING; NONHUMAN; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION;

AMINO ACID SEQUENCE; ANTIMICROBIAL CATIONIC PEPTIDES; ANTINEOPLASTIC AGENTS; CELL LINE, TUMOR; CELL PROLIFERATION; CELL SURVIVAL; CLASSICAL SWINE FEVER VIRUS; DRUG SCREENING ASSAYS, ANTITUMOR; ESCHERICHIA COLI; HUMANS; MICROBIAL SENSITIVITY TESTS; RECOMBINANT FUSION PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; FUNGI;

EID: 78449272347     PISSN: 09394451     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00726-010-0625-0     Document Type: Article

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