TrxA mediating fusion expression of antimicrobial peptide CM4 from multiple joined genes in Escherichia coli

Protein Expression and Purification

Volumn 64, Issue 2, 2009, Pages 225-230

  Zhou, Liangfan ^a   Zhao, Zhihui ^a   Li, Baocun ^a   Cai, Yufeng ^a   Zhang, Shuangquan ^a  

^a NANJING NORMAL UNIVERSITY   (China)

Author keywords

Antimicrobial peptide CM4; Hydroxylamine hydrochloride; Multimers; TrxA

Indexed keywords

ANTIMICROBIAL CATIONIC PEPTIDE; CM4 PEPTIDE, BOMBYX MORI; ESCHERICHIA COLI PROTEIN; HYBRID PROTEIN; THIOREDOXIN; TRXA PROTEIN, E COLI;

ANIMAL; ARTICLE; BIOSYNTHESIS; CHEMISTRY; ESCHERICHIA COLI; GENE VECTOR; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; SILKWORM;

ANIMALS; ANTIMICROBIAL CATIONIC PEPTIDES; BOMBYX; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENETIC VECTORS; RECOMBINANT FUSION PROTEINS; THIOREDOXINS;

BOMBYX MORI; ESCHERICHIA COLI;

EID: 58749104161     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2008.11.006     Document Type: Article

References (19)

1. Boman H.G. Peptide antibiotics and their role in innate immunity. Annu. Rev. Immunol. 13 (1995) 61-92
2. Koczulla A.R., and Bals R. Antimicrobial peptides: current status and therapeutic potential. Drugs 63 (2003) 389-406
3. Joerger R.D. Alternatives to antibiotics: bacteriocins, antimicrobial peptides and bacteriophages. Poult. Sci. 82 (2003) 640-647
4. Hancock R.E. Peptide antibiotics. Lancet 349 (1997) 418-422
5. Merrifield R.B. Solid phase peptide synthesis, I, the synthesis of a tetrapeptide. J. Am. Chem. Soc. 85 (1963) 2149-2154
6. Hancock R.E., and Sahl H.G. Antimicrobial and host-defense peptides as new anti-infective therapeutic strategies. Nat. Biotechnol. 24 (2006) 1551-1557
7. Ingham A.B., and Moore R.J. Recombinant production of antimicrobial peptides in heterologous microbial systems. Biotechnol. Appl. Biochem. 47 (2007) 1-9
8. Morassutti C., De Amicis F., Bandiera A., and Marchetti S. Expression of SMAP-29 cathelicidin-like peptide in bacterial cells by intein-mediated system. Protein Expr. Purif. 39 (2005) 160-168
9. Rao X.C., Li S., Hu J.C., Jin X.L., Hu X.M., Huang J.J., Chen Z.J., Zhu J.M., and Hu F.Q. A novel carrier molecule for high-level expression of peptide antibiotics in Escherichia coli. Protein Expr. Purif. 36 (2004) 11-18
10. Jin Z., Han Y.P., Zhang W.H., Wang F., Zhang S.Q., and Mao Y.M. Ultrastructural observation of E. coli K12 treated with antibacterial peptide CM4. Shi Yan Sheng Wu Xue Bao 33 (2000) 89-95
11. Xu J., and Zhang S.Q. The research of the mechanism of antibacterial peptide CM4 component against A. parasiticus. Prog. Nat. Sci. 11 (2001) 1263-1267
12. Xu J., and Zhang S.Q. The mechanism of antibacterial of antibacterial peptide CM4 component was observed by confocal laser scanning microscope. Prog. Nat. Sci. 10 (2001) 1105-1109
13. Sambrook J., and Russell D.W. Molecular Cloning: A Laboratory Manual. third ed. (2001), Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
14. Moks T., Abrahmsén L., Holmgren E., Bilich M., Olsson A., Uhlén M., Pohl G., Sterky C., Hultberg H., and Josephson S. Expression of human insulin-like growth factor I in bacteria: use of optimized gene fusion vectors to facilitate protein purification. Biochemistry 26 (1987) 5239-5244
15. Chen Y.Q., Zhang S.Q., Li B.C., Qiu W., Jiao B., Zhang J., and Diao Z.Y. Expression of a cytotoxic cationic antibacterial peptide in Escherichia coli using two fusion partners. Protein Expr. Purif. 57 (2008) 303-311
16. Lee D.G., Park Y., Kim H.N., Kim H.K., Kim P.I., Choi B.H., and Hahm K.S. Antifungal mechanism of an antimicrobial peptide, HP (2-20), derived from N-terminus of Helicobacter pylori ribosomal protein L1 against Candida albicans. Biochem. Biophys. Res. Commun. 291 (2002) 1006-1013
17. Iost I., Guillerez J., and Dreyfus M. Bacteriophage T7 RNA polymerase travels far ahead of ribosomes in vivo. J. Bacteriol. 174 (1992) 619-622
18. Iost I., and Dreyfus M. The stability of Escherichia coli lacZ mRNA depends upon the simultaneity of its synthesis and translation. EMBO J. 14 (1995) 3252-3261
19. Zhong Z.X., Xu Z.N., Peng L., Huang L., Fang X.M., and Cen P.L. Tandem repeat mhBD2 gene enhance the soluble fusion expression of hBD2 in Escherichia coli. Appl. Microbiol. Biotechnol. 71 (2006) 661-667