Silencing of Plum pox virus 5′UTR/P1 sequence confers resistance to a wide range of PPV strains

Plant Cell Reports

Volumn 29, Issue 12, 2010, Pages 1435-1444

  Di Nicola Negri, Elisa ^a   Tavazza, Mario ^b   Salandri, Laura ^a   Ilardi, Vincenza ^a  

^a CRA   (Italy)

^b ENEA C R CASACCIA   (Italy)

Author keywords

Pathogen derived resistance; Potyvirus; PPV; RNAi; Sharka

Indexed keywords

PLANT RNA; PRIMER DNA; VIRUS DNA;

5' UNTRANSLATED REGION; ARTICLE; GENE SILENCING; GENETICS; MOSAIC VIRUS; NUCLEOTIDE SEQUENCE; PHYLOGENY; POLYMERASE CHAIN REACTION; TOBACCO; TRANSGENIC PLANT;

5' UNTRANSLATED REGIONS; BASE SEQUENCE; DNA PRIMERS; DNA, VIRAL; GENE SILENCING; PHYLOGENY; PLANTS, GENETICALLY MODIFIED; PLUM POX VIRUS; POLYMERASE CHAIN REACTION; RNA, PLANT; TOBACCO;

NICOTIANA BENTHAMIANA; PLUM POX VIRUS; POTYVIRUS; PRUNUS;

EID: 78349311037     PISSN: 07217714     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00299-010-0933-6     Document Type: Article

References (54)

1. Barajas D, Tenllado F, Gonzales-Jara P, Martinez-Garcia B, Atencio FA, Diaz-Ruiz JR (2004) Resistance to Plum pox virus (PPV) in Nicotiana benthamiana plants transformed with the PPV HC-Pro silencing suppressor gene. J Plant Pathol 86: 239-248.
2. Barba M, Hadidi A, Candresse T, Cambra M (2010) Plum pox virus. In: Hadidi A, Barba M, Candresse T, Jelkmann W (eds) Virus and virus-like diseases of pome and stone fruits. The American Phytopathological Society Press, St Paul, MN, USA (in press).
3. Bau HJ, Cheng YH, Yu TA, Yang JS, Yeh SD (2003) Broad-spectrum resistance to different geographic strains of Papaya ringspot virus in coat protein gene transgenic papaya. Phytopathol 93: 112-120.
4. Brodersen P, Sakvarelidze-Achard L, Bruun-Rasmussen M, Dunoyer P, Yamamoto YY, Sieburth L, Voinnet O (2008) Widespread translational inhibition by plant miRNAs and siRNAs. Science 32: 1185-1190.
5. Cambra M, Capote N, Myrta A, Llácer G (2006) Plum pox virus and the estimated costs associated with sharka disease. EPPO Bull 36: 202-204.
6. Candresse T, Cambra M (2006) Causal agent of sharka disease: historical perspective and current status of Plum pox virus strains. EPPO Bull 36: 239-246.
7. Candresse T, Cambra M, Dallot S, Lanneau M, Asensio M, Gorris MT, Revers F, Macquaire G, Olmos A, Boscia D, Quiot JB, Dunez J (1998) Comparison of monoclonal antibodies and polymerase chain reaction assays for the typing of isolates belonging to the D and M serotypes of plum pox potyvirus. Phytopathol 88: 198-204.
8. Decroocq V, Foulongne M, Lambert P, Le Gall O, Mantin C, Pascal T, Schurdi-Levraud V, Kervella J (2005) Analogues of virus resistance genes map to QTLs for resistance to sharka disease in Prunus davidiana. Mol Gen Genome 272: 680-689.
9. Di Nicola-Negri E, Brunetti A, Tavazza M, Ilardi V (2005) Hairpin RNA-mediated silencing of Plum pox virus P1 and HC-Pro genes for efficient and predictable resistance to the virus. Transgenic Res 14: 989-994.
10. Fanigliulo A, Comes S, Maiss E, Piazzolla P, Crescenzi A (2003) The complete nucleotide sequence of Plum pox virus isolates from sweet (PPV-SwC) and sour (PPV-SoC) cherry and their taxonomic relationships within the species. Arch Virol 148: 2137-2153.
11. García JA, Cambra M (2007) Plum pox virus and sharka disease. Plant Viruses 1: 69-79.
12. Glasa M, Palkovics L, Komínek P, Labonne G, Pittnerová S, Kúdela O, Candresse T, Subr Z (2004) Geographically and temporally distant natural recombinant isolates of Plum pox virus are genetically very similar and form a unique PPV subgroup. J Gen Virol 85: 2671-2681.
13. Guo HS, Garcia JA (1997) Delayed resistance to Plum pox potyvirus mediated by a mutated RNA replicase gene: involvement of a gene-silencing mechanism. Mol Plant Microbe Interact 10: 160-170.
14. Guo HS, Cervera MT, Garcia JA (1998) Plum pox poptyvirus resistance associated to transgene silencing that can be stabilized after different number of plant generations. Gene 206: 263-272.
15. Guo HS, Lopez-Moya JJ, Garcia JA (1999) Mitotic stability of infection-induced resistance to Plum pox potyvirus associated with transgene silencing and DNA methylation. Mol Plant Microbe Interact 12: 103-111.
16. Hily JM, Scorza R, Webb K, Ravelonandro M (2005) Accumulation of the long class of siRNA is associated with resistance to Plum pox virus in transgenic woody perennial plum tree. Mol Plant Microbe Interact 18: 794-799.
17. Hily JM, Ravelonandro M, Damsteegt V, Bassett C, Petri C, Liu Z, Scorza R (2007) Plum pox virus coat protein gene intron hairpin RNA construct provides resistance to Plum pox virus in Nicotiana benthamiana and Prunus domestica. J Am Soc Hort Sci 132: 850-858.
18. Ilardi V, Mazzei M, Loreti S, Tomassoli L, Barba M (1995) Biomolecular and serological methods to identify CMV strains on tomato. EPPO Bull 25: 321-327.
19. Ilardi V, Di Nicola-Negri E, Brunetti A, Gentile A, Monticelli S, Damiano C (2007) RNA interference for sharka disease resistance. Acta Hort 738: 593-599.
20. Ion-Nagy L, Lansac M, Eyquard JP, Salvador B, Garcia JA, Le Gall O, Hernould M, Schurdi-Levraud V, Decroocq V (2006) PPV long-distance movement is occasionally permitted in resistant apricot hosts. Virus Res 120: 70-78.
21. Jacquet C, Ravelonandro M, Bachelier JC, Dunez J (1998) High resistance to Plum pox virus (PPV) in transgenic plants containing modified and truncated form of PPV coat protein gene. Transgenic Res 7: 29-39.
22. James D, Varga A (2005) Nucleotide sequence analysis of Plum pox virus isolate W3174: evidence of a new strain. Virus Res 110: 143-150.
23. Karayiannis I, Thomidis T, Tsaftaris A (2008) Inheritance of resistance to Plum pox virus in apricot (Prunus armeniaca L). Tree Genet Genome 4: 143-148.
24. Kegler H, Fuchs E, Gruntzig M, Schwarz S (1998) Some results of 50 years' research on resistance to Plum pox potyvirus. Arch Phytopath Pflanz 31: 479-506.
25. Lennefors BL, van Roggen PM, Yndgaard F, Savenkov EI, Valkonen JPT (2008) Efficient dsRNA-mediated transgenic resistance to Beet necrotic yellow vein virus in sugar beets is not affected by other soilborne and aphid-transmitted viruses. Transgenic Res 17: 219-228.
26. Liu Z, Scorza R, Hily J-M, Scott SW, James D (2007) Engineering resistance to multiple Prunus fruit viruses through expression of chimeric hairpins. J Am Soc Hort Sci 132: 407-414.
27. Lopez-Moya JJ, Fernandez-Fernandez MR, Cambra M, Garcia JA (2000) Biotechnological aspects of Plum pox virus. J Biotech 76: 121-136.
28. Lu S, Shi R, Tsao C, Yi X, Li L, Chiang VL (2004) RNA silencing in plants by the expression of siRNA duplexes. Nucleic Acids Res 32(21): e171. doi: 10. 1093/nar/gnh170.
29. N strains that carry highly homologous P1 sequences and recover transgene expression at infection. Mol Plant Microbe Interact 13: 66-373.
30. Missiou A, Kalantidis K, Boutla A, Tzortzakaki S, Tabler M, Tsagris M (2004) Generation of transgenic potato plants highly resistant to Potato virus Y (PVY) through RNA silencing. Mol Breed 14: 185-197.
31. Nemchinov L, Crescenzi A, Hadidi A, Piazzolla P, Verderevskaya T (1998) Present status of the new cherry subgroup of Plum pox virus (PPV-C). In: Hadidi A, Khetarpal RK, Koganezawa H (eds) Plant virus disease control. APS Press, St Paul, Minnesota, pp 629-638.
32. Németh M (1994) History and importance of plum pox in stone-fruit production. EPPO Bull 24: 525-536.
33. Nomura K, Ohshima K, Anai T, Uekusa H, Kita N (2004) RNA silencing of the introduced coat protein gene of Turnip mosaic virus confers broad-spectrum resistance in transgenic Arabidopsis. Phytopathol 94: 730-736.
34. Pandolfini T, Molesini B, Avesani L, Spena A, Polverari A (2003) Expression of self-complementary hairpin RNA under the control of the rolC promoter confers systemic disease resistance to Plum pox virus without preventing local infection. BMC Biotechnol 3: 7.
35. Pei Y, Tuschl T (2006) On the art of identifying effective and specific siRNAs. Nat Methods 3: 670-676.
36. Petri C, Burgos L (2005) Transformation of fruit trees. Useful breeding tool or continued future prospect? Transgenic Res 14: 15-26.
37. Polak J, Pivalova J, Kundu J, Jokes M, Scorza R, Ravelonandro R (2008) Behaviour of transgenic Plum pox virus-resistant Prunus domestica L clone C-5 grown in the open field under a high and permanent infection pressure of the PPV-Rec strain. J Plant Pathol 90: S133-S136.
38. Ravelonandro M, Monsion M, Delbos R, Dunez J (1993) Variable resistance to Plum pox virus and potato virus Y infection in transgenic plants expressing Plum pox virus coat protein. Plant Sci 91: 157-169.
39. Reynolds A, Leake D, Boese Q, Scaringe S, Marshall WS, Khvorova A (2004) Rational siRNA design for RNA interference. Nat Biotech 22: 326-330.
40. Rubio M, Martínez-Gómez P, Dicenta F (2003) Resistance of almond cultivars to Plum pox virus (sharka). Plant Breed 122: 462-464.
41. Ruiz-Ferrer V, Voinnet O (2009) Roles of plant small RNAs in biotic stress responses. Annu Rev Plant Biol 60: 485-510.
42. Salamon P, Palkovics L (2002) Characterization of Plum pox virus PPV-BT-H isolated from naturally infected blackthorn (Prunus spinosa L) in Hungary. Eur J Plant Pathol 108: 903-907.
43. Scorza R, Callahan A, Levy L, Damsteegt V, Webb K, Ravelonandro M (2001) Post-transcriptional gene silencing in Plum pox virus resistant transgenic European plum containing the Plum pox potyvirus coat protein gene. Trasgenic Res 10: 201-209.
44. Serçe CU, Candresse T, Svanella-Dumas L, Krizbai L, Gazel M, Caglayan K (2009) Further characterization of a new recombinant group of Plum pox virus isolates, PPV-T, found in orchards in the Ankara province of Turkey. Virus Res 142: 121-126.
45. Shao Y, Chan CY, Maliyekkel A, Lawrence CE, Roninson IB, Ding Y (2007) Effect of target secondary structure on RNAi efficiency. RNA 13: 1631-1640.
46. Smith NA, Singh SP, Wang MB, Stoutjesdijk PA, Green AG, Waterhouse PM (2000) Total silencing by intron-spliced hairpin RNAs. Nature 407: 319-320.
47. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4. 0. Mol Biol Evol 24: 1596-1599.
48. Ui-Tei K, Naito Y, Takahashi F, Haraguchi T, Ohki-Hamazaki H, Juni A, Ueda R, Saigo K (2004) Guidelines for the selection of highly efficient siRNA sequences for mammalian and chick RNA interference. Nucl Acids Res 32: 936-948.
49. Voinnet O (2005) Induction and suppression of RNA silencing: insights from viral infections. Nat Rev Genet 6: 206-220.
50. Waterhouse PM, Wang MB, Lought T (2001) Gene silencing as an adaptive defence against viruses. Nature 411: 834-842.
51. Wetzel T, Candresse T, Ravelonandro M, Dunez J (1991) A polymerase chain reaction assay adapted to Plum pox virus detection. J Virol Methods 33: 355-365.
52. Wittner A, Palkovics L, Balazs E (1998) Nicotiana benthamiana plants transformed with the Plum pox virus helicase gene are resistant to virus infection. Virus Res 53: 97-103.
53. Xu L, Song YZ, Zhu JH, Guo XQ, Zhu CX, Wen FJ (2009) Conserved sequences of replicase gene-mediated resistance to Potyvirus through RNA silencing. J Plant Biol 52: 550-559.
54. Yuan B, Latek R, Hossbach M, Tuschl T, Lewitter F (2004) siRNA Selection Server: an automated siRNA oligonucleotide prediction server. Nucl Acids Res 32: 130-134.