Silencing STAT3 with short hairpin RNA enhances radiosensitivity of human laryngeal squamous cell carcinoma xenografts in vivo

Experimental and Therapeutic Medicine

Volumn 1, Issue 6, 2010, Pages 947-953

  Li, Xiaoming ^a   Wang, Hairu ^a,b   Lu, Xiuying ^a   Di, Bin ^a  

^a BETHUNE INTERNATIONAL PEACE HOSPITAL   (China)

^b The Pepole's Hospital of Haikou   (China)

Author keywords

Laryngeal neoplasms; Radiosensitivity; RNA interference; Signal transducer and activator of transcription 3; Xenograft tumor

Indexed keywords

PROTEIN BCL 2; SHORT HAIRPIN RNA; STAT3 PROTEIN; VASCULOTROPIN;

ANIMAL EXPERIMENT; ANIMAL MODEL; APOPTOSIS; ARTICLE; CONTROLLED STUDY; DOWN REGULATION; DRUG EFFECT; LARYNX CARCINOMA; MALE; MOUSE; NONHUMAN; PROTEIN EXPRESSION; RADIOSENSITIVITY; SQUAMOUS CELL CARCINOMA; TUMOR XENOGRAFT;

EID: 78049308479     PISSN: 17920981     EISSN: 17921015     Source Type: Journal    
DOI: 10.3892/etm.2010.156     Document Type: Article

References (29)

1. Rhee JG, Li D, O'Malley BW Jr and Suntharalingam M: Combination radiation and adenovirus-mediated P16(INK4A) gene therapy in a murine model for head and neck cancer. ORLJ Otorhinolaryngol Relat Spec 65: 144-154, 2003.
2. Rhee JG, Li D, Suntharalingam M, Guo C, O'Malley BW Jr and Carney JP: Radiosensitization of head/neck squamous cell carcinoma by adenovirus-mediated expression of the Nbs1 protein. Int J Radiat Oncol Biol Phys 67: 273-278, 2007.
3. Takeda K, Noguchi K, Shi W, et al: Targeted disruption of the mouse Stat3 gene leads to early embryonic lethality. Proc Natl Acad Sci USA94: 3801-3804, 1997.
4. Fukada T, Ohtani T, Yoshida Y, et al: STAT3 orchestrates contradictory signals in cytokine-induced G1 to S cell-cycle transition. EMBOJ 17: 6670-6677, 1998.
5. Catlett-Falcone R, Landowski TH, Oshiro MM, et al: Constitutive activation of Stat3 signaling confers resistance to apoptosis in human U266 myeloma cells. Immunity 10: 105-115, 1999.
6. Bromberg JF: Activation of STATproteins and growth control. Bioessays 23: 161-169, 2001.
7. Niu G, Wright KL, Ma Y, et al: Role of Stat3 in regulating p53 expression and function. Mol Cell Biol 25: 7432-7440, 2005.
8. Odajima J, Matsumura I, Sonoyama J, et al: Full oncogenic activities of v-Src are mediated by multiple signaling pathways. Ras as an essential mediator for cell survival. J Biol Chem 275: 24096-24105, 2000.
9. Ning ZQ, Li J, McGuinness Mand Arceci RJ: STAT3 activation is required for Asp(816) mutant c-Kit induced tumorigenicity. Oncogene 20: 4528-4536, 2001.
10. Bowman T, Broome MA, Sinibaldi D, et al: Stat3-mediated Myc expression is required for Src transformation and PDGF-induced mitogenesis. Proc Natl Acad Sci USA98: 7319-7324, 2001.
11. Sinibaldi D, Wharton W, Turkson J, Bowman T, Pledger WJ and Jove R: Induction of p21WAF1/CIP1 and cyclin D1 expression by the Src oncoprotein in mouse fibroblasts: role of activated STAT3 signaling. Oncogene 19: 5419-5427, 2000.
12. Karni R, Jove R and Levitzki A: Inhibition of pp60c-Src reduces Bcl-XLexpression and reverses the transformed phenotype of cells overexpressing EGF and HER-2 receptors. Oncogene 18: 4654-4662, 1999.
13. Aoki Y, Feldman GMand Tosato G: Inhibition of STAT3 signaling induces apoptosis and decreases survivin expression in primary effusion lymphoma. Blood 101: 1535-1542, 2003.
14. Gritsko T, Williams A, Turkson J, et al: Persistent activation of stat3 signaling induces survivin gene expression and confers resistance to apoptosis in human breast cancer cells. Clin Cancer Res 12: 11-19, 2006.
15. Epling-Burnette PK, Liu JH, Catlett-Falcone R, et al: Inhibition of STAT3 signaling leads to apoptosis of leukemic large granular lymphocytes and decreased Mcl-1 expression. J Clin Invest 107: 351-362, 2001.
16. Wei D, Le X, Zheng L, et al: Stat3 activation regulates the expression of vascular endothelial growth factor and human pancreatic cancer angiogenesis and metastasis. Oncogene 22: 319-329, 2003.
17. Li X, Wang H, Lu Xand Di B: STAT3 blockade with shRNAenhances radiosensitivity in Hep-2 human laryngeal squamous carcinoma cells. Oncol Rep 23: 345-353, 2010.
18. Bissery MC, Guenard D, Gueritte-Voegelein F and Lavelle F: Experimental antitumor activity of taxotere (RP56976, NSC628503), a taxol analogue. Cancer Res 51: 4845-4852, 1991.
19. Crisby M, Nordin-Fredriksson G, Shah PK, Yano J, Zhu J and Nilsson J: Pravastatin treatment increases collagen content and decreases lipid content, inflammation, metalloproteinases, and cell death in human carotid plaques: implications for plaque stabilization. Circulation 103: 926-933, 2001.
20. Weidner N, Semple JP, Welch WRand Folkman J: Tumor angiogenesis and metastasis - correlation in invasive breast carcinoma. NEngl J Med 324: 1-8, 1991.
21. Turkson J and Jove R: STATproteins: novel molecular targets for cancer drug discovery. Oncogene 19: 6613-6626, 2000.
22. Bharti AC, Shishodia S, Reuben JM, et al: Nuclear factor-kappaB and STAT3 are constitutively active in CD138+ cells derived from multiple myeloma patients, and suppression of these transcription factors leads to apoptosis. Blood 103: 3175-3184, 2004.
23. Greten FR, Weber CK, Greten TF, et al: Stat3 and NF-kappaB activation prevents apoptosis in pancreatic carcinogenesis. Gastroenterology 123: 2052-2063, 2002.
24. Real PJ, Sierra A, De Juan A, Segovia JC, Lopez-Vega JM and Fernandez-Luna JL: Resistance to chemotherapy via Stat3-dependent overexpression of Bcl-2 in metastatic breast cancer cells. Oncogene 21: 7611-7618, 2002.
25. Otero DC, Poli V, David Mand Rickert RC: Cutting edge: inherent and acquired resistance to radiation-induced apoptosis in B cells: a pivotal role for STAT3. J Immunol 177: 6593-6597, 2006.
26. Calvin DP, Nam S, Buettner R, Sekharam M, Torres-Roca J and Jove R: Inhibition of STAT3 activity with STAT3 antisense oligonucleotide (STAT3-ASO) enhances radiation induced apoptosis in DU145 prostate cancer cells. Int J Radiat Oncol Biol Phys 57: S297, 2003.
27. Zhang YC, Taylor MM, Samson WK and Phillips MI: Antisense inhibition: oligonucleotides, ribozymes, and siRNAs. Methods Mol Med 106: 11-34, 2005.
28. Amin HM, McDonnell TJ, Ma Y, et al: Selective inhibition of STAT3 induces apoptosis and G(1) cell cycle arrest in ALK-positive anaplastic large cell lymphoma. Oncogene 23: 5426-5434, 2004.
29. Niu G, Wright KL, Huang M, et al: Constitutive Stat3 activity up-regulates VEGF expression and tumor angiogenesis. Oncogene 21: 2000-2008, 2002.