Label-free liquid chromatography-tandem mass spectrometry analysis with automated phosphopeptide enrichment reveals dynamic human milk protein phosphorylation during lactation

Analytical Biochemistry

Volumn 408, Issue 1, 2011, Pages 136-146

  Froehlich, John W ^a   Chu, Caroline S ^a   Tang, Ning ^b   Waddell, Keith ^b   Grimm, Rudolf ^b   Lebrilla, Carlito B ^a,c  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b AGILENT TECHNOLOGIES   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Label free; Milk; Phosphoprotein; Proteomics; Quantification

Indexed keywords

CASEIN; CELL SIGNALING; DRUG PRODUCTS; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PHOSPHORYLATION; PROTEOMICS; TITANIUM DIOXIDE;

COEFFICIENTS OF VARIATIONS; ENRICHMENT OF PHOSPHOPEPTIDES; LABEL FREE; LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY; MILK; PHOSPHOPROTEIN; POST-TRANSLATIONAL MODIFICATIONS; QUANTIFICATION;

PROTEINS;

ADIPOPHILIN; ALPHA CASEIN; ALPHA LACTALBUMIN; APOLIPOPROTEIN A2; BETA CASEIN; CHORDIN; CLUSTERIN; HAPTOGLOBIN; LACTOFERRIN; MILK PROTEIN; MUCIN 1; MYOSIN VI; OSTEOPONTIN; PHOSPHOPEPTIDE; SECRETORY COMPONENT; SERUM ALBUMIN; TITANIUM DIOXIDE; UBIQUITIN;

AMINO ACID SEQUENCE; ARTICLE; BREAST MILK; HUMAN; HUMAN EXPERIMENT; LACTATION; LIQUID CHROMATOGRAPHY; NORMAL HUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN DETERMINATION; PROTEIN PHOSPHORYLATION; TANDEM MASS SPECTROMETRY; UNINDEXED SEQUENCE;

EID: 77958459734     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.08.031     Document Type: Article

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