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Volumn 16, Issue 38, 2010, Pages 11572-11575

A doubly signal-amplified DNA detection method based on pre-complexed [Ru(bpy)3]2+-doped silica nanoparticles

Author keywords

Biochips; DNA detection; Microarrays; Nanoparticles; Signal amplification

Indexed keywords

DNA DETECTION; ENHANCED SENSITIVITY; RU(BPY); SIGNAL AMPLIFICATION; SILICA NANOPARTICLES;

EID: 77957587777     PISSN: 09476539     EISSN: 15213765     Source Type: Journal    
DOI: 10.1002/chem.201001255     Document Type: Article
Times cited : (19)

References (37)
  • 12
    • 0037012674 scopus 로고    scopus 로고
    • Angew. Chem. Int. Ed. 2002, 41, 1548-1551.
    • (2002) Angew. Chem. Int. Ed. , vol.41 , pp. 1548-1551
  • 34
    • 84874204734 scopus 로고    scopus 로고
    • In general, fluorescent intensity profiles of DNA detection show a sigmoidal curve to the concentration of target DNA. With the single amplification method, fluorescent intensities for the concentrations of 100 fm through 100 am would fall below the dynamic range, and therefore, the fluorescent signals for those target concentrations were indistinguishable. We assume that by amplifying the fluorescent intensities several fold using doubly amplified preorganized RSNPs, the fluorescent intensities of target DNA in concentrations of 100 fm through 100 am come into the dynamic range, which allows the highly increased detection limit
    • In general, fluorescent intensity profiles of DNA detection show a sigmoidal curve to the concentration of target DNA. With the single amplification method, fluorescent intensities for the concentrations of 100 fm through 100 am would fall below the dynamic range, and therefore, the fluorescent signals for those target concentrations were indistinguishable. We assume that by amplifying the fluorescent intensities several fold using doubly amplified (preorganized) RSNPs, the fluorescent intensities of target DNA in concentrations of 100 fm through 100 am come into the dynamic range, which allows the highly increased detection limit.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.