An ertapenem-resistant extended-spectrum-β-lactamase-producing Klebsiella pneumoniae clone carries a novel OmpK36 porin variant

Antimicrobial Agents and Chemotherapy

Volumn 54, Issue 10, 2010, Pages 4178-4184

  García Fernández, Aurora ^a   Miriagou, Vivi ^b   Papagiannitsis, Costas C ^b   Giordano, Alessandra ^c   Venditti, Mario ^c   Mancini, Carlo ^c   Carattoli, Alessandra ^a  

^a ISTITUTO SUPERIORE DI SANITÀ   (Italy)

^b HELLENIC PASTEUR INSTITUTE   (Greece)

^c SAPIENZA UNIVERSITY OF ROME   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

CARBAPENEM; ERTAPENEM; EXTENDED SPECTRUM BETA LACTAMASE; IMIPENEM; MEROPENEM; PORIN;

ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; ENZYME ANALYSIS; GENE AMPLIFICATION; GENE EXPRESSION; GENE LOSS; GENE SEQUENCE; GENETIC SCREENING; GENETIC VARIABILITY; KLEBSIELLA PNEUMONIAE; MOLECULAR CLONING; NONHUMAN; NONSENSE MUTATION; NUCLEOTIDE SEQUENCE; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN VARIANT; PULSED FIELD GEL ELECTROPHORESIS;

ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; BETA-LACTAMASES; BETA-LACTAMS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; IMIPENEM; KLEBSIELLA PNEUMONIAE; MOLECULAR SEQUENCE DATA; PORINS; THIENAMYCINS;

EID: 77957348328     PISSN: 00664804     EISSN: 10986596     Source Type: Journal    
DOI: 10.1128/AAC.01301-09     Document Type: Article

References (42)

1. Albertí, S., F. Rodríguez-Quinones, T. Schirmer, G. Rummel, J. M. Tomás, J. P. Rosenbusch, and V. J. Benedí. 1995. A porin from Klebsiella pneumoniae: sequence homology, three-dimensional model, and complement binding. Infect. Immun. 63:903-910.
2. Arlet, G., M. Rouveau, and A. Philippon. 1997. Substitution of alanine for aspartate at position 179 in the SHV-6 extended-spectrum β-lactamase. FEMS Microbiol. Lett. 152:163-167.
3. Bredin, J., N. Saint, M. Malléa, E. Dé, G. Molle, J. M. Pagès, and V. Simonet. 2002. Alteration of pore properties of Escherichia coli OmpF induced by mutation of key residues in anti-loop 3 region. Biochem. J. 363:521-528.
4. Carattoli, A. 2009. Resistance plasmid families in Enterobacteriaceae. Antimicrob. Agents Chemother. 53:2227-2238.
5. Carattoli, A., A. Bertini, L. Villa, V. Falbo, K. L. Hopkins, and E. J. Threlfall. 2005. Identification of plasmids by PCR-based replicon typing. J. Microbiol. Methods 63:219-228.
6. Carattoli, A., A. García-Fernández, P. Varesi, D. Fortini, S. Gerardi, A. Penni, C. Mancini, and A. Giordano. 2008. Molecular epidemiology of Escherichia coli producing extended-spectrum β-lactamases isolated in Rome, Italy. J. Clin. Microbiol. 46:103-108.
7. Clinical and Laboratory Standards Institute. 2009. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard, 8th ed. CLSI document M7-A8. Clinical and Laboratory Standards Institute, Wayne, PA.
8. Crowley, B., V. J. Benedí, and A. Domènech-Sánchez. 2002. Expression of SHV-2 β-lactamase and of reduced amounts of OmpK36 porin in Klebsiella pneumoniae results in increased resistance to cephalosporins and carbapenems. Antimicrob. Agents Chemother. 46:3679-3682.
9. Dé, E., A. Basle, M. Jaquinod, N. Saint, M. Malléa, G. Molle, and J. M. Pagès. 2001. A new mechanism of antibiotic resistance in Enterobacteriaceae induced by a structural modification of the major porin. Mol. Microbiol. 41:189-198.
10. Diancourt, L., V. Passet, J. Verhoef, P. A. Grimont, and S. Brisse. 2005. Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates. J. Clin. Microbiol. 43:4178-4182.
11. Doménech-Sánchez, A., L. Martínez-Martínez, S. Hernández-Allés, M. del Carmen Conejo, A. Pascual, J. M. Tomás, S. Albertí, and V. J. Benedí. 2003. Role of Klebsiella pneumoniae OmpK35 porin in antimicrobial resistance. Antimicrob. Agents Chemother. 47:3332-3335.
12. Doumith, M., M. J. Ellington, D. M. Livermore, and N. Woodford. 2009. Molecular mechanisms disrupting porin expression in ertapenem-resistant Klebsiella and Enterobacter spp. clinical isolates from the UK. J. Antimicrob. Chemother. 63:659-667.
13. Dutzler, R., G. Rummel, S. Alberti, S. Hernández-Alles, P. Phale, J. Rosenbusch, V. Benedí, and T. Schirmer. 1999. Crystal structure and functional characterization of OmpK36, the osmoporin of Klebsiella pneumoniae. Structure 7:425-434.
14. Elliott, E., A. J. Brink, J. van Greune, Z. Els, N. Woodford, J. Turton, M. Warner, and D. M. Livermore. 2006. In vivo development of ertapenem resistance in a patient with pneumonia caused by Klebsiella pneumoniae with an extended-spectrum β-lactamase. Clin. Infect. Dis. 42:e95-e98.
15. Gröbner, S., D. Linke, W. Schütz, C. Fladerer, J. Madlung, I. B. Autenrieth, W. Witte, and Y. Pfeifer. 2009. Emergence of carbapenem-non- susceptible extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolates at the university hospital of Tubingen, Germany. J. Med. Microbiol. 58:912-922.
16. Hasdemir, U. O., J. Chevalier, P. Nordmann, and J. M. Pagès. 2004. Detection and prevalence of active drug efflux mechanism in various multidrugresistant Klebsiella pneumoniae strains from Turkey. J. Clin. Microbiol. 42:2701-2706.
17. Hernández-Allés, S., S. Albertí, D. Alvarez, A. Doménech-Sánchez, L. Martínez-Martínez, J. Gil, J. M. Tomás, and V. J. Benedí. 1999. Porin expression in clinical isolates of Klebsiella pneumoniae. Microbiology 145:673-679.
18. Jacoby, G. A., D. M. Mills, and N. Chow. 2004. Role of β-lactamases and porins in resistance to ertapenem and other β-lactams in Klebsiella pneumoniae. Antimicrob. Agents Chemother. 48:3203-3206.
19. James, C. E., K. R. Mahendran, A. Molitor, J. M. Bolla, A. N. Bessonov, M. Winterhalter, and J. M. Pagès. 2009. How β-lactam antibiotics enter bacteria: a dialogue with the porins. PLoS One 4:e5453.
20. Jeanteur, D., J. H. Lakey, and F. Pattus. 1991. The bacterial porin superfamily: sequence alignment and structure prediction. Mol. Microbiol. 5:2153-2164.
21. ACT-1 β-lactamase production, porin OmpK35/36 insertional inactivation, and down-regulation of the phosphate transport porin PhoE. Antimicrob. Agents Chemother. 50:3396-3406.
22. Kitchel, B., J. K. Rasheed, J. B. Patel, A. Srinivasan, S. Navon-Venezia, Y. Carmeli, A. Brolund, and C. G. Giske. 2009. Molecular epidemiology of KPC-producing Klebsiella pneumoniae isolates in the United States: clonal expansion of multilocus sequence type 258. Antimicrob. Agents Chemother. 53:3365-3370.
23. Lartigue, M. F., L. Poirel, C. Poyart, H. Réglier-Poupet, and P. Nordmann. 2007. Ertapenem resistance of Escherichia coli. Emerg. Infect. Dis. 13:315-317.
24. Leavitt, A., I. Chmelnitsky, R. Colodner, I. Ofek, Y. Carmeli, and S. Navon-Venezia. 2009. Ertapenem resistance among extended-spectrum-β- lactamase- producing Klebsiella pneumoniae isolates. J. Clin. Microbiol. 47:969-974.
25. Lee, Y. H., B. Cho, I. K. Bae, C. L. Chang, and S. H. Jeong. 2006. Klebsiella pneumoniae strains carrying the chromosomal SHV-11 β-lactamase gene produce the plasmid-mediated SHV-12 extended-spectrum β-lactamase more frequently than those carrying the chromosomal SHV-1 β-lactamase gene. J. Antimicrob. Chemother. 57:1259-1261.
26. Livermore, D. M. 2002. The impact of carbapenemases on antimicrobial development and therapy. Curr. Opin. Invest. Drugs 3:218-224.
27. Livermore, D. M., R. Canton, M. Gniadkowski, P. Nordmann, G. M. Rossolini, G. Arlet, J. Ayala, T. M. Coque, I. Kern-Zdanowicz, F. Luzzaro, L. Poirel, and N. Woodford. 2007. CTX-M: changing the face of ESBLs in Europe. J. Antimicrob. Chemother. 59:165-174.
28. Mabilat, C., S. Goussard, W. Sougakoff, R. C. Spencer, and P. Courvalin. 1990. Direct sequencing of the amplified structural gene and promoter for the extended-broad-spectrum β-lactamase TEM-9 (RHH-1) of Klebsiella pneumoniae. Plasmid 23:27-34.
29. Martínez-Martínez, L. 2008. Extended-spectrum β-lactamases and the permeability barrier. Clin. Microbiol. Infect. 14(Suppl. 1):82-89.
30. Martínez-Martínez, L., S. Hernández-Allés, S. Albertí, J. M. Tomás, V. J. Benedí, and G. A. Jacoby. 1996. In vivo selection of porin-deficient mutants of Klebsiella pneumoniae with increased resistance to cefoxitin and expanded- spectrum cephalosporins. Antimicrob. Agents Chemother. 40:342-348.
31. Mena, A., V. Plasencia, L. García, O. Hidalgo, J. I. Ayestaran, S. Albertí, N. Borrell, J. L. Pérez, and A. Oliver. 2006. Characterization of a large outbreak by CTX-M-1-producing Klebsiella pneumoniae and mechanisms leading to in vivo carbapenem resistance development. J. Clin. Microbiol. 44:2831-2837.
32. Pérez-Pérez, F. J., and N. D. Hanson. 2002. Detection of plasmid-mediated AmpC β-lactamase genes in clinical isolates by using multiplex PCR. J. Clin. Microbiol. 40:2153-2162.
33. Poirel, L., C. Heritier, V. Tolun, and P. Nordmann. 2004. Emergence of oxacillinase-mediated resistance to imipenem in Klebsiella pneumoniae. Antimicrob. Agents Chemother. 48:15-22.
34. Rasheed, J. K., J. W. Biddle, K. F. Anderson, L. Washer, C. Chenoweth, J. Perrin, D. W. Newton, and J. B. Patel. 2008. Detection of the Klebsiella pneumoniae carbapenemase type 2 carbapenem-hydrolyzing enzyme in clinical isolates of Citrobacter freundii and K. oxytoca carrying a common plasmid. J. Clin. Microbiol. 46:2066-2069.
35. Szabo, D., F. Silveira, A. M. Hujer, R. A. Bonomo, K. M. Hujer, J. W. Marsh, C. R. Bethel, Y. Doi, K. Deeley, and D. L. Paterson. 2006. Outer membrane protein changes and efflux pump expression together may confer resistance to ertapenem in Enterobacter cloacae. Antimicrob. Agents Chemother. 50:2833-2835.
36. Thiolas, A., C. Bornet, A. Davin-Régli, J. M. Pagès, and C. Bollet. 2004. Resistance to imipenem, cefepime, and cefpirome associated with mutation in Omp36 osmoporin of Enterobacter aerogenes. Biochem. Biophys. Res. Commun. 317:851-856. (Pubitemid 38481450)
37. Turton, J. F., M. E. Kaufmann, M. Warner, J. Coelho, L. Dijkshoorn, T. van der Reijden, and T. L. Pitt. 2004. A prevalent, multiresistant clone of Acinetobacter baumannii in Southeast England. J. Hosp. Infect. 58:170-179.
38. Walsh, T. R., M. A. Toleman, L. Poirel, and P. Nordmann. 2005. Metallo- β-lactamases: the quiet before the storm? Clin. Microbiol. Rev. 18:306-325.
39. Wang, X. D., J. C. Cai, H. W. Zhou, R. Zhang, and G. X. Chen. 2009. Reduced susceptibility to carbapenems in Klebsiella pneumoniae clinical isolates associated with plasmid-mediated β-lactamases production and OmpK36 porin deficiency. J. Med. Microbiol. 58:1196-1202.
40. Weldhagen, G. F., and A. Prinsloo. 2004. Molecular detection of GES-2 extended spectrum beta-lactamase producing Pseudomonas aeruginosa in Pretoria, South Africa. Int. J. Antimicrob. Agents 24:35-38.
41. Woodford, N., E. J. Fagan, and M. J. Ellington. 2006. Multiplex PCR for rapid detection of genes encoding CTX-M extended-spectrum β-lactamases. J. Antimicrob. Chemother. 57:154-155.
42. Yigit, H., A. M. Queenan, G. J. Anderson, A. Domènech- Sánchez, J. W. Biddle, C. D. Steward, S. Albertí, K. Bush, and F. C. Tenover. 2001. Novel carbapenem-hydrolyzing β-lactamase, KPC-1, from a carbapenem-resistant strain of Klebsiella pneumoniae. Antimicrob. Agents Chemother. 45:1151-1161.