A novel real-time PCR assay for specific detection and quantification of Mycobacterium avium subsp. paratuberculosis in milk with the inherent possibility of differentiation between viable and dead cells

BMC Research Notes

Volumn 3, Issue , 2010, Pages

  Dzieciol, Monika ^a   Volgger, Patrick ^a   Khol, Johannes ^a   Baumgartner, Walter ^a   Wagner, Martin ^a   Hein, Ingeborg ^a  

^a UNIVERSITY OF VETERINARY MEDICINE   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA (MICROORGANISMS); BOVIDAE; CORYNEBACTERINEAE; MYCOBACTERIUM; MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS;

EID: 77957307886     PISSN: None     EISSN: 17560500     Source Type: Journal    
DOI: 10.1186/1756-0500-3-251     Document Type: Article

References (32)

1. Antibacterial activities of naturally occurring compounds against Mycobacterium avium subsp. paratuberculosis. SYY Wong IR Grant M Friedman C Elliott C Situ, Appl Environ Microbiol 2008 74 5986 5990 10.1128/AEM.00981-08 18676709
2. Mycobacterium avium subspecies paratuberculosis, Crohn's disease and the Doomsday Scenario. J Hermon-Taylor, Gut Pathogens 2009 1 1 15 10.1186/1757-4749-1-15 19602288
3. Mycobacterium avium subspecies paratuberculosis and its relationship with Crohn's disease. JL Mendoza R Lana M Díaz-Rubio, World J Gastroenterol 2009 28 417 422 10.3748/wjg.15.417
4. Development of an F57 sequence-based real-time PCR assay for detection of Mycobacterium avium subsp. paratuberculosis in milk. T Tasara R Stephan, Appl Environ Microbiol 2005 71 5957 5968 10.1128/AEM.71.10.5957-5968.2005 16204510
5. Effect of chemical decontamination and refrigerated storage on the isolation of Mycobacterium avium subsp. paratuberculosis from heat-treated milk. IR Grant MT Rowe, Lett Appl Microbiol 2004 38 283 288 10.1111/j.1472-765X.2004. 01498.x 15214726
6. Mycobacterium avium subsp. paratuberculosis in cow bulk tank milk in Cyprus detected by culture and quantitative IS900 and F57 real-time PCR. I Slana M Liapi M Moravkova A Kralova I Pavlik, Prev Vet Med 2009 89 223 226 10.1016/j.prevetmed.2009.02.020 19349086
7. Application of an F57 sequence-based real-time PCR assay for Mycobacterium paratuberculosis detection in bulk tank raw milk and slaughtered healthy dairy cows. C Bosshard R Stephan T Tasara, J Food Prot 2006 69 1662 1667 16865901
8. Development of a nested PCR method targeting a unique multicopy element, ISMap02, for detection of Mycobacterium avium subsp. paratuberculosis in fecal samples. JR Stabel JP Bannantine, J Clin Microbiol 2005 43 4744 4750 10.1128/JCM.43.9.4744-4750.2005 16145136
9. New triplex real-time PCR assay for detection of Mycobacterium avium subsp. paratuberculosis in bovine feces. H Schönenbrücher A Abdulmawjood K Failing M Bülte, Appl Environ Microbiol 2008 74 2751 2758 10.1128/AEM.02534-07 18326682
10. Real-time PCR, compared to liquid and solid culture media and ELISA, for the detection of Mycobacterium avium ssp. paratuberculosis. CA Alinovi MP Ward TL Lin GE Moore CC Wu, Vet Microbiol 2009 136 177 179 10.1016/j.vetmic.2008.10. 012 19091493
11. Mycobacteria distinct from Mycobacterium avium subsp. paratuberculosis isolated from the faeces of ruminants possess IS900-like sequences detectable by IS900 polymerase chain reaction: implications for diagnosis. DV Cousins R Whittington I Marsh A Masters RJ Evans P Kluver, Mol Cell Probes 1999 14 431 442 10.1006/mcpr.1999.0275 (Pubitemid 30088436)
12. An IS900-like sequence found in Mycobacterium sp. other than Mycobacterum avium subsp. paratuberculosis. S Englund G Bölske KE Johansson, FEMS Microbiol Lett 2002 209 267 271 10.1111/j.1574-6968.2002.tb11142.x 12007816
13. Development and evaluation of a Mycobacterium avium subspecies paratuberculosis (MAP) specific multiplex PCR assay. T Tasara LE Hoelzle R Stephan, Int J Food Microbiol 2005 104 279 287 10.1016/j.ijfoodmicro.2005.03.009 15982769 (Pubitemid 41443248)
14. Putative in vitro expressed gene fragments unique to Mycobacterium avium subspecies paratuberculosis. KK Nielsen P Ahrens, FEMS Microbiol Lett 2002 214 199 203 10.1111/j.1574-6968.2002.tb11347.x 12351231
15. Development of matrix lysis for concentration of gram positive bacteria from food and blood. P Rossmanith B Süss M Wagner I Hein, J Microbiol Methods 2007 69 504 511 10.1016/j.mimet.2007.03.003 17462766
16. IS900 PCR to detect Mycobacterium paratuberculosis in retail supplies of whole pasteurized cows' milk in England and Wales. D Millar J Ford J Sanderson S Withey M Tizard T Doran J Hermon-Taylor, Appl Environ Microbiol 1996 62 3446 3452 8795236
17. Real-time PCR method with statistical analysis to compare the potential of DNA isolation methods to remove PCR inhibitors from samples for diagnostic PCR. G Flekna W Schneeweiss FJ Smulders M Wagner I Hein, Mol Cell Probes 2007 21 182 187 10.1016/j.mcp.2007.02.001 17178212
18. Mfold web server for nucleic acid folding and hybridization prediction. M Zuker, Nucl Acids Res 2003 31 3406 3415 10.1093/nar/gkg595 12824337
19. A novel real-time PCR assay for the simultaneous detection and differentiation of Clostridium chauvoei and Clostridium septicum in the context of clostridial myonecrosis. A Halm M Wagner J Koefer I Hein, J Clin Microbiol 2010 48 1093 1098 10.1128/JCM.01975-09 20129968
20. Mycobacterium porcinum strains isolated from bovine bulk milk: Implications for Mycobacterium avium subsp. paratuberculosis detection by PCR and culture. R Taddei I Barbieri ML Pacciarini F Fallacara GL Belletti N Arrigoni, Vet Microbiol 2008 130 338 347 10.1016/j.vetmic.2008.02.007 18378411
21. Sensitive detection of Mycobacterium avium subsp. paratuberculosis in bovine semen by real-time PCR. D Herthnek S Englund PTJ Willemsen G Bölske, J Appl Microbiol 2006 100 1095 1102 10.1111/j.1365-2672.2006.02924.x 16630010
22. Automated high-throughput immunomagnetic separation-PCR for detection of Mycobacterium avium subsp. paratuberculosis in bovine milk. C Metzger-Boddien D Khaschabi M Schönbauer S Boddien T Schlederer J Kehle, Int J Food Microbiol 2006 110 201 208 10.1016/j.ijfoodmicro.2006.01.042 16814891
23. Incidence of Mycobacterium paratuberculosis in bulk raw and commercially pasteurized cows'milk from approved dairy processing establishments in the United Kingdom. IR Grant HJ Ball MT Rowe, Appl Environ Microbiol 2002 68 2428 2435 10.1128/AEM.68.5.2428-2435.2002 11976118
24. Surveillance of bulk raw and commercially pasteurized cow's milk from approved irish liquid-milk pasteurization plants to determine the incidence of Mycobacterium paratuberculosis. CE O'Reilly L O'Connor W Anderson P Harvey IR Grant J Donaghy M Rowe P O'Mahony, Appl Environ Microbiol 2004 70 5138 5144 10.1128/AEM.70.9.5138-5144.2004 15345392
25. A robust method for bacterial lysis and DNA purification to be used with real-time PCR for detection of Mycobacterium avium subsp. paratuberculosis in milk. D Herthnek SS Nielsen A Lindberg G Bölske, J Microbiol Methods 2008 75 335 340 10.1016/j.mimet.2008.07.009 18694788
26. Rapid real-time PCR assay for detection and quantitation of Mycobacterium avium subsp. paratuberculosis DNA in artificially contaminated milk. J O'Mahony C Hill, Appl Environ Microbiol 2004 70 4561 4568 10.1128/AEM.70.8.4561-4568. 2004 15294786
27. On-farm spread of Mycobacterium avium subsp. paratuberculosis in raw milk studied by IS900 and F57 competitive real time quantitative PCR and culture examination. I Slana P Kralik A Kralova I Pavlik, Int J Food Microbiol 2008 128 250 257 10.1016/j.ijfoodmicro.2008.08.013 18824269
28. An inter-laboratory ring trial for the detection and isolation of Mycobacterium avium subsp. paratuberculosis from raw milk artificially contaminated with naturally infected faeces. JA Donaghy MT Rowe JLW Rademaker P Hammer L Herman V DeJonghe B Blanchard K Duhem E Vindel, Food Microbiol 2008 25 128 135 10.1016/j.fm.2007.06.007 17993386
29. Real-time PCR-based methods for detection Mycobacterium avium subsp. paratuberculosis in water and milk. D Rodríguez-Lzaro M D'Agostino A Herrewegh M Pla N Cook J Ikonomopoulos, Int J Food Microbiol 2005 101 93 104 10.1016/j.ijfoodmicro.2004.09.005 15878410
30. Application of IS900 PCR for detection of Mycobacterium avium subsp. paratuberculosis directly from raw milk. SR Pillai BM Jayarao, J Dairy Sci 2002 85 1052 1057 10.3168/jds.S0022-0302(02)74165-9 12086038
31. Association among results of serum ELISA, faecal culture and nested PCR on milk, blood and faeces for the detection of paratuberculosis in dairy cows. PJ Pinedo DO Rae JE Williams GA Donovan P Melendez CD Buergelt, Transbound Emerg Dis 2008 55 125 133 10.1111/j.1865-1682.2007.01009.x 18397500
32. Genome and transcriptome scale portrait of sigma factors in Mycobacterium avium subsp. paratuberculosis. LA Sechi GE Felis N Amed D Paccagnini D Usai S Ortu P Molicotti S Zanetti, Infect Genet Evol 2007 7 424 432 10.1016/j.meegid.2007.01.001 17292677