Purification and characterization of a novel endo-β-1,4-glucanase, AfEG22, from the giant snail, Achatina fulica frussac

Acta Biochimica et Biophysica Sinica

Volumn 42, Issue 10, 2010, Pages 729-734

  Teng, Yigang ^a   Yin, Qiuyu ^a   Ding, Ming ^b   Zhao, Fukun ^a,b  

^a INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY   (China)

^b ZHEJIANG SCI TECH UNIVERSITY   (China)

Author keywords

Achatina fulica ferussac; Congo red staining; endo 1,4 glucanase; purification; thermostability

Indexed keywords

CARBOXYMETHYLCELLULOSE; CELLULASE; ENDO 1,3(4) BETA GLUCANASE;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BIOCATALYSIS; CHEMISTRY; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYMOLOGY; GENETICS; HYDROLYSIS; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; MOLECULAR GENETICS; MOLECULAR WEIGHT; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SNAIL; TEMPERATURE;

AMINO ACID SEQUENCE; ANIMALS; BIOCATALYSIS; CARBOXYMETHYLCELLULOSE SODIUM; CELLULASE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDO-1,3(4)-BETA-GLUCANASE; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; SEQUENCE ANALYSIS, PROTEIN; SEQUENCE HOMOLOGY, AMINO ACID; SNAILS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SUBSTRATE SPECIFICITY; TEMPERATURE;

ACHATINA FULICA; GASTROPODA;

EID: 77957299034     PISSN: 16729145     EISSN: 17457270     Source Type: Journal    
DOI: 10.1093/abbs/gmq083     Document Type: Article

References (28)

1. Bayer EA, Chanzy H, Lamed R and Shoham Y. Cellulose, cellulases and cellulosomes. Curr Opin Struct Biol 1998, 8: 548-557.
2. Beguin P and Aubert JP. The biological degradation of cellulose. FEMS Microbiol Rev 1994, 13: 25-58.
3. Bhat MK. Cellulases and related enzymes in biotechnology. Biotechnol Adv 2000, 18: 355-383.
4. Jeon E, Hyeon J, Eun LS, Park BS, Kim SW, Lee J and Han SO. Cellulosic alcoholic fermentation using recombinant Saccharomyces cerevisiae engineered for the production of Clostridium cellulovorans endoglucanase and Saccharomycopsis fibuligera beta-glucosidase. FEMS Microbiol Lett 2009, 301: 130-136.
5. Yanase S, Yamada R, Kaneko S, Noda H, Hasunuma T, Tanaka T and Ogino C, et al. Ethanol production from cellulosic materials using cellulase-expressing yeast. Biotechnol J 2010, 5: 449-4556.
6. Li WY, Wang J, Li YH, Ding M, Xu GJ, Liu LY and Zhao FK. pH-dependent stability of EGX, a multi-functional cellulase from mollusca, Ampullaria crossean. Acta Biochim Biophys Sin 2004, 36: 603-608.
7. Li Y, Yin Q, Ding M and Zhao F. Purification, characterization and molecular cloning of a novel endo-beta-1,4-glucanase AC-EG65 from the mollusc Ampullariacrossean. Comp Biochem Physiol B Biochem Mol Biol 2009, 153: 149-156.
8. Li YH, Guo R, Yin QY, Ding M, Zhang SL, Xu GJ and Zhao FK. Purification and characterization of two endo-beta-1,4-glucanases from mollusca, Ampullaria crossean. Acta Biochim Biophys Sin 2005, 37: 702-708.
9. Watanabe H and Tokuda G. Cellulolytic systems in insects. Annu Rev Entomol 2010, 55: 609-632.
10. Nishida Y, Suzuki K, Kumagai Y, Tanaka H, Inoue A and Ojima T. Isolation and primary structure of a cellulase from the Japanese sea urchin Strongylocentrotus nudus. Biochimie 2007, 89: 1002-1011.
11. Suzuki K, Ojima T and Nishita K. Purification and cDNA cloning of a cellulase from abalone Haliotis discus hannai. Eur J Biochem 2003, 270: 771-778.
12. Xu B, Hellman U, Ersson B and Janson JC. Purification, characterization and amino-acid sequence analysis of a thermostable, low molecular mass endo-beta-1,4-glucanase from blue mussel, Mytilus edulis. Eur J Biochem 2000, 267: 4970-4977.
13. Ding M, Teng Y, Yin Q, Chen W and Zhao F. Identification, expression, and characterization of the highly conserved D-xylose isomerase in animals. Acta Biochim Biophys Sin 2009, 41: 116-122.
14. Sakamoto K and Toyohara H. A comparative study of cellulase and hemicellulase activities of brackish water clam Corbicula japonica with those of other marine Veneroida bivalves. J Exp Biol 2009, 212: 2812-2818.
15. Sakamoto K and Toyohara H. Molecular cloning of glycoside hydrolase family 45 cellulase genes from brackish water clam Corbicula japonica. Comp Biochem Physiol B Biochem Mol Biol 2009, 152: 390-396.
16. Raut SK and Barker GM. Achatina fulica Bowdich and other Achatinidae as pests in tropical agriculture. In: Barker GM ed. Molluscs as Crop Pests. Wallingford: CABI Publishing, 2002.
17. Maeda I, Shimohigashi Y, Kihara H and Ohno M. Purification and characterization of a cellulase from the giant snail Achatina fulica. Biosci Biotechnol Biochem 1996, 60: 122-124.
18. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227: 680-685.
19. Lowry OH, Rosebrough NJ, Farr AL and Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951, 193: 265-275.
20. Wood TM, Bhat KM, Willis AW and Scott TK. Methods for measuring cellulase activities. Methods in Enzymology. San Diego: Academic Press, 1988, 87-112.
21. Guo R, Ding M, Zhang S, Xu G and Zhao F. Expression and characterization of two secreted His6-tagged endo-beta-1,4-glucanases from the mollusc Ampullaria crossean in Pichia pastoris. Acta Biochim Biophys Sin 2008, 40: 419-425.
22. Guo R, Ding M, Zhang SL, Xu GJ and Zhao FK. Molecular cloning and characterization of two novel cellulase genes from the mollusc Ampullaria crossean. J Comp Physiol B 2008, 178: 209-215.
23. Bok JD, Yernool DA and Eveleigh DE. Purification, characterization, and molecular analysis of thermostable cellulases CelA and CelB from Thermotoga neapolitana. Appl Environ Microbiol 1998, 64: 4774-4781.
24. Bronnenmeier K, Kern A, Liebl W and Staudenbauer WL. Purification of Thermotoga maritima enzymes for the degradation of cellulosic materials. Appl Environ Microbiol 1995, 61: 1399-1407.
25. Zverlov V, Mahr S, Riedel K and Bronnenmeier K. Properties and gene structure of a bifunctional cellulolytic enzyme (CelA) from the extreme thermophile 'Anaerocellum thermophilum' with separate glycosyl hydrolase family 9 and 48 catalytic domains. Microbiology 1998, 144: 457-465.
26. Hreggvidsson GO, Kaiste E, Holst O, Eggertsson G, Palsdottir A and Kristjansson JK. An extremely thermostable cellulase from the thermophilic eubacterium rhodothermus marinus. Appl Environ Microbiol 1996, 62: 3047-3049.
27. Huang Y, Krauss G, Cottaz S, Driguez H and Lipps G. A highly acidstable and thermostable endo-beta-glucanase from the thermoacidophilic archaeon Sulfolobus solfataricus. Biochem J 2005, 385: 581-588.
28. Shanmughapriya S, Kiran GS, Selvin J, Thomas TA and Rani C. Optimization, purification, and characterization of extracellular mesophilic alkaline cellulase from sponge-associated Marinobacter sp. MSI032. Appl Biochem Biotechnol 2010, 162: 625-640.