Active site threonine facilitates proton transfer during dioxygen activation at the diiron center of toluene/o-xylene monooxygenase hydroxylase

Journal of the American Chemical Society

Volumn 132, Issue 39, 2010, Pages 13582-13585

  Song, Woon Ju ^a   McCormick, Michael S ^a   Behan, Rachel K ^a   Sazinsky, Matthew H ^b   Jiang, Wei ^c   Lin, Jeffery ^b   Krebs, Carsten ^c   Lippard, Stephen J ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^b POMONA COLLEGE   (United States)

^c PENNSYLVANIA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE; AROMATIC SUBSTRATES; DECAY KINETICS; DIOXYGEN ACTIVATION; DIOXYGENS; HYDROXYLASES; MONOOXYGENASES; REACTION CONDITIONS; REGIOSPECIFICITY; SITE DIRECTED MUTAGENESIS; SSBAUER SPECTROSCOPIES; STOPPED FLOW; X RAY CRYSTAL STRUCTURES;

AROMATIC HYDROCARBONS; ENZYMES; PHOSPHATASES; PROTON TRANSFER; XYLENE;

REACTION INTERMEDIATES;

AROMATIC COMPOUND; ENZYME VARIANT; IRON; MUTANT PROTEIN; ORTHO XYLENE MONOOXYGENASE HYDROXYLASE; OXYGEN; OXYGENASE; THREONINE; TOLUENE MONOOXYGENASE HYDROXYLASE; UNCLASSIFIED DRUG; UNSPECIFIC MONOOXYGENASE;

ARTICLE; CRYSTAL STRUCTURE; ENZYME ACTIVATION; KINETICS; MOSSBAUER SPECTROSCOPY; OXIDATION; PROTON TRANSPORT; SITE DIRECTED MUTAGENESIS; STEADY STATE; STEREOSPECIFICITY; ULTRAVIOLET SPECTROSCOPY;

CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; FERRIC COMPOUNDS; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; OXYGEN; OXYGENASES; PROTONS; THREONINE;

EID: 77957297019     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja1063795     Document Type: Article

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