Development of an efficient E. coli expression and purification system for a catalytically active, human Cullin3-RINGBox1 protein complex and elucidation of its quaternary structure with Keap1

Biochemical and Biophysical Research Communications

Volumn 400, Issue 4, 2010, Pages 471-475

  Small, Evan ^a   Eggler, Aimee ^b   Mesecar, Andrew D ^b  

^a UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

^b PURDUE UNIVERSITY   (United States)

Author keywords

Codon optimized; Cullin3; Keap1; Nrf2; Quaternary structure; Rbx1; Stoichiometry

Indexed keywords

BACTERIAL PROTEIN; CULLIN; CULLIN3; KELCH LIKE ECH ASSOCIATED PROTEIN 1; RINGBOX1 PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; COMPLEX FORMATION; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN QUATERNARY STRUCTURE; STOICHIOMETRY; ULTRACENTRIFUGATION;

CARRIER PROTEINS; CATALYSIS; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; CULLIN PROTEINS; ESCHERICHIA COLI; HUMANS; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; PROTEIN STRUCTURE, QUATERNARY; UBIQUITINATION; ULTRACENTRIFUGATION;

ESCHERICHIA COLI;

EID: 77957280953     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2010.08.062     Document Type: Article

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