High-level expression and purification of recombinant SCF ubiquitin ligases

Methods in Enzymology

Volumn 398, Issue , 2005, Pages 125-142

  Li, Ti ^a   Pavletich, Nikola P ^a   Schulman, Brenda A ^a   Zheng, Ning ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

CULLIN; F BOX PROTEIN; PROTEIN; PROTEIN RBX1; RECOMBINANT PROTEIN; S PHASE KINASE ASSOCIATED PROTEIN; UBIQUITIN PROTEIN LIGASE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIUM; CHEMICAL ANALYSIS; ENZYME PURIFICATION; INSECT CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; STRUCTURE ANALYSIS;

BACTERIA (MICROORGANISMS); INSECTA;

EID: 27644598123     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(05)98012-9     Document Type: Review

References (19)

1. Bai C., Sen P., Hofmann K., Ma L., Goebl M., Harper J.W., and Elledge S.J. SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box Cell 86 1996 263 274
2. Deshaies R.J. SCF and cullin/Ring H2-based ubiquitin ligases Annu. Rev. Cell. Dev. Biol. 15 1999 435 467
3. Feldman R.M., Correll C.C., Kaplan K.B., and Deshaies R.J. A complex of Cdc4p, Skp1p, and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p Cell 91 1997 221 230
4. Furukawa M., He Y.J., Borchers C., and Xiong Y. Targeting of protein ubiquitination by BTB-Cullin 3-Roc1 ubiquitin ligases Nat. Cell Biol. 5 2003 1001 1007
5. Furukawa M., Ohta T., and Xiong Y. Activation of UBC5 ubiquitin-conjugating enzyme by the RING finger of ROC1 and assembly of active ubiquitin ligases by all cullins J. Biol. Chem. 277 2002 15758 15765
6. Geyer R., Wee S., Anderson S., Yates J., and Wolf D.A. BTB/POZ domain proteins are putative substrate adaptors for cullin 3 ubiquitin ligases Mol. Cell 12 2003 783 790
7. Kamura T., Koepp D.M., Conrad M.N., Skowyra D., Moreland R.J., Iliopoulos O., Lane W.S., Kaelin W.G. Jr., Elledge S.J., Conaway R.C., Harper J.W., and Conaway J.W. Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase Science 284 1999 657 661
8. Kamura T., Sato S., Haque D., Liu L., Kaelin W.G. Jr., Conaway R.C., and Conaway J.W. The Elongin BC complex interacts with the conserved SOCS-box motif present in members of the SOCS, ras, WD-40 repeat, and ankyrin repeat families Genes Dev. 12 1998 3872 3881
9. Kipreos E.T., Lander L.E., Wing J.P., He W.W., and Hedgecock E.M. cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family Cell 85 1996 829 839
10. Ohta T., Michel J.J., Schottelius A.J., and Xiong Y. ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity Mol. Cell 3 1999 535 541
11. Pintard L., Willis J.H., Willems A., Johnson J.L., Srayko M., Kurz T., Glaser S., Mains P.E., Tyers M., Bowerman B., and Peter M. The BTB protein MEL-26 is a substrate-specific adaptor of the CUL-3 ubiquitin-ligase Nature 425 2003 311 316
12. Schulman B.A., Carrano A.C., Jeffrey P.D., Bowen Z., Kinnucan E.R., Finnin M.S., Elledge S.J., Harper J.W., Pagano M., and Pavletich N.P. Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex Nature 408 2000 381 386
13. Seol J.H., Feldman R.M., Zachariae W., Shevchenko A., Correll C.C., Lyapina S., Chi Y., Galova M., Claypool J., Sandmeyer S., Nasmyth K., and Deshaies R.J. Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34 Genes Dev. 13 1999 1614 1626
14. Skowyra D., Craig K.L., Tyers M., Elledge S.J., and Harper J.W. F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex Cell 91 1997 209 219
15. Skowyra D., Koepp D.M., Kamura T., Conrad M.N., Conaway R.C., Conaway J.W., Elledge S.J., and Harper J.W. Reconstitution of G1 cyclin ubiquitination with complexes containing SCFGrr1 and Rbx1 Science 284 1999 662 665
16. Tan P., Fuchs S.Y., Chen A., Wu K., Gomez C., Ronai Z., and Pan Z.Q. Recruitment of a ROC1-CUL1 ubiquitin ligase by Skp1 and HOS to catalyze the ubiquitination of I kappa B alpha Mol. Cell 3 1999 527 533
17. Wu G., Xu G., Schulman B.A., Jeffrey P.D., Harper J.W., and Pavletich N.P. Structure of a beta-TrCP1-Skp1-beta-catenin complex: Destruction motif binding and lysine specificity of the SCF(beta-TrCP1) ubiquitin ligase Mol. Cell 11 2003 1445 1456
18. Xu L., Wei Y., Reboul J., Vaglio P., Shin T.H., Vidal M., Elledge S.J., and Harper J.W. BTB proteins are substrate-specific adaptors in an SCF-like modular ubiquitin ligase containing CUL-3 Nature 425 2003 316 321
19. Zheng N., Schulman B.A., Song L., Miller J.J., Jeffrey P.D., Wang P., Chu C., Koepp D.M., Elledge S.J., Pagano M., Conaway R.C., Conaway J.W., Harper J.W., and Pavletich N.P. Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF ubiquitin ligase complex Nature 416 2002 703 709