Identification and expression analysis of connexin-45 and connexin-60 as major connexins in porcine oocytes

Journal of Animal Science

Volumn 88, Issue 10, 2010, Pages 3269-3279

  Nitta, M ^a   Yogo, K ^b   Ohashi, M ^a   Akiyama, M ^a   Kunitomo, Y ^a   Ogawa, T ^a   Ishida Kitagawa, N ^a   Miyoshi, J ^c   Sato, E ^d   Takeya, T ^a  

^a NARA INSTITUTE OF SCIENCE AND TECHNOLOGY   (Japan)

^b SHIZUOKA UNIVERSITY   (Japan)

^c OSAKA MEDICAL CENTER FOR CANCER AND CARDIOVASCULAR DISEASES   (Japan)

^d TOHOKU UNIVERSITY   (Japan)

Author keywords

Connexin 37; Connexin 45; Connexin 60; Oocyte complementary deoxyribonucleic acid library; Ovarian follicle; Porcine oocyte

Indexed keywords

CONNEXIN 37; CONNEXIN 45; GAP JUNCTION PROTEIN;

ANIMAL; ANIMAL DISEASE; ARTICLE; BIOSYNTHESIS; CHEMISTRY; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE; GENE EXPRESSION PROFILING; GENE LIBRARY; IN SITU HYBRIDIZATION; MOLECULAR CLONING; NUCLEIC ACID AMPLIFICATION; OOCYTE; PHYSIOLOGY; POLYMERASE CHAIN REACTION; SWINE;

ANIMALS; CLONING, MOLECULAR; CONNEXINS; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE; GENE EXPRESSION PROFILING; GENE LIBRARY; IN SITU HYBRIDIZATION; NUCLEIC ACID AMPLIFICATION TECHNIQUES; OOCYTES; POLYMERASE CHAIN REACTION; SWINE;

MAMMALIA; SUS;

EID: 77957246052     PISSN: 00218812     EISSN: 15253163     Source Type: Journal    
DOI: 10.2527/jas.2009-2781     Document Type: Article

References (37)

1. Asklund, T., I. B. Appelskog, O. Ammerpohl, I. A. Langmoen, M. S. Dilber, A. Aints, T. J. Ekström, and P. M. Almqvist. 2003. Gap junctionmediated bystander effect in primary cultures of human malignant gliomas with recombinant expression of the HSVtk gene. Exp. Cell Res. 284:185-195.
2. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. A. Smith, J. G. Seidman, and K. Struhl. 1988. Current Protocols in Molecular Biology, Suppl. 7. Wiley Interscience, New York, NY.
3. Baldridge, D., F. Lecanda, C.-S. Shin, J. Stains, and R. Civitelli. 2001. Sequence and structure of the mouse connexin 45 gene. Biosci. Rep. 21:683-689.
4. Borowczyk, E., M. L. Johnson, J. J. Bilski, P. Borowicz, D. A. Redmer, L. P. Reynolds, and A. T. Grazul-Bilska. 2006. Gap junctional connexin 37 is expressed in sheep ovaries. Endocrine 30:223-230.
5. Cottrell, G. T., and J. M. Burt. 2005. Functional consequences of heterogeneous gap junction channel formation and its influence in health and disease. Biochim. Biophys. Acta 1711:126-141.
6. Cruciani, V., and S. O. Mikalsen. 2006. The vertebrate connexin family. Cell. Mol. Life Sci. 63:1125-1140.
7. Dbouk, H. A., R. M. Mroue, M. E. El-Sabban, and R. S. Talhouk. 2009. Connexins: A myriad of functions extending beyond assembly of gap junction channel. Cell Commun. Signal. 7:4.
8. Elenes, S., A. D. Martinez, M. Delmar, E. C. Beyer, and A. P. Moreno. 2001. Heterotypic docking of Cx43 and Cx45 connexons blocks fast voltage gating of Cx43. Biophys. J. 81:1406-1418.
9. Eppig, J. J. 1991. Intercommunication between mammalian oocytes and companion follicle cells. Bioessays 13:569-574.
10. Fortune, J. E. 1994. Ovarian follicular growth and development in mammals. Biol. Reprod. 50:225-232.
11. Gershon, E., V. Plaks, and N. Dekel. 2008. Gap junctions in the ovary: Expression, localization and function. Mol. Cell. Endocrinol. 282:18-25.
12. Gittens, J. E., and G. M. Kidder. 2005. Differential contributions of connexin 37 and connexin 43 to oogenesis revealed in chimeric reaggregated mouse ovaries. J. Cell Sci. 118:5071-5078.
13. Goldberg, G. S., V. Valiunas, and P. R. Brink. 2004. Selective permeability of gap junction channels. Biochim. Biophys. Acta 1662:96-101.
14. Granot, I., E. Bechor, A. Barash, and N. Dekel. 2002. Connexin 43 in rat oocytes: Developmental modulation of its phosphorylation. Biol. Reprod. 66:568-573.
15. Grapes, L., Y. Zhang, and M. F. Rothschild. 2002. Rapid communication: Physical and linkage mapping of the porcine connexin 37 (CX37) gene. J. Anim. Sci. 80:1375-1376.
16. Gray, S. A., M. A. Mannan, and P. J. O'Shaughnessy. 1996. Development of cytochrome P450 17 alpha-hydroxylase (P450c17) mRNA and enzyme activity in neonatal ovaries of normal and hypogonadal (hpg) mice. J. Mol. Endocrinol. 17:55-60.
17. Griffin, J., B. R. Emery, I. Huang, C. M. Peterson, and D. T. Carrell. 2006. Comparative analysis of follicle morphology and oocyte diameter in four mammalian species (mouse, hamster, pig, and human). J. Exp. Clin. Assist. Reprod. 3:2 doi:10.1186/1743-1050-3-2.
18. Hombach, S., U. Janssen-Bienhold, G. Söhl, T. Schubert, H. Bussow T. Ott, R. Weiler, and K. Willecke. 2004. Functional expression of connexin 57 in horizontal cells of the mouse retina. Eur. J. Neurosci. 29:2633-2640.
19. Itahana, K., Y. Morikazu, and T. Takeya. 1996. Differential expression of four connexin genes, Cx-26, Cx-30.3, Cx-32, and Cx-43, in the porcine ovarian follicle. Endocrinology 137:5036-5044.
20. Itahana, K., T. Tanaka, Y. Morikazu, S. Komatu, N. Ishida, and T. Takeya. 1998. Isolation and characterization of a novel connexin gene, Cx60, in porcine ovarian follicles. Endocrinology 139:320-329.
21. Juneja, S. C., K. J. Barr, G. C. Enders, and G. M. Kidder. 1999. Defects in the germ line and gonads of mice lacking connexin43. Biol. Reprod. 60:1263-1270.
22. Kidder, G. M., and A. A. Mhawi. 2002. Gap junctions and ovarian folliculogenesis. Reproduction 123:613-620.
23. Li, T. Y., D. Colley, K. J. Barr, S. P. Yee, and G. M. Kidder. 2007. Rescue of oogenesis in Cx37-null mutant mice by oocyte-specific replacement with Cx43. J. Cell Sci. 120:4117-4125.
24. Manthey, D., F. Bukauskas, C. G. Lee, C. A. Kozak, and K. Willecke. 1999. Molecular cloning and functional expression of the mouse gap junction gene connexin-57 in human HeLa cells. J. Biol. Chem. 274:14716-14723.
25. McEvoy, T. G., G. D. Coull, P. J. Broadbent, J. S. M. Hutchinson, and B. K. Speake. 2000. Fatty acid composition of lipids in immature cattle, pig and sheep oocytes with intact zona pellucida. J. Reprod. Fertil. 118:163-170.
26. Meşe, G., G. Richard, and T. W. White. 2007. Gap junctions: Basic structure and function. J. Invest. Dermatol. 127:2516-2524.
27. Nuttinck, F., N. Peynot, P. Humblot, A. Massip, F. Dessy, and J. E. Fléchon. 2000. Comparative immunohistochemical distribution of connexin 37 and connexin 43 throughout folliculogenesis in the bovine ovary. Mol. Reprod. Dev. 57:60-66.
28. Petters, R. M., and K. D. Wells. 1993. Culture of pig embryos. J. Reprod. Fertil. Suppl. 48:61-73.
29. Simon, A. M., H. Chen, and C. L. Jackson. 2006. Cx37 and Cx43 localize to zona pellucida in mouse ovarian follicles. Cell Commun. Adhes. 13:61-77.
30. Simon, A. M., D. A. Goodenough, E. Li, and D. L. Paul. 1997. Female infertility in mice lacking connexin37. Nature 385:525-529.
31. Teilmann, S. C. 2005. Differential expression and localization of connexin-37 and connexin-43 in follicles of different stages in the 4-week-old mouse ovary. Mol. Cell. Endocrinol. 234:27-35.
32. Tong, D., J. E. Gittens, G. M. Kidder, and D. Bai. 2006. Patchclamp study reveals that the importance of connexin 43-mediated gap junctional communication for ovarian folliculogenesis is strain specific in the mouse. Am. J. Physiol. Cell Physiol. 290:C290-C297.
33. Veitch, G. I., J. E. I. Gittens, Q. Shao, D. W. Laird, and G. M. Kidder. 2004. Selective assembly of connexin37 into heterocellular gap junctions at the oocyte/granulosa cell interface. J. Cell Sci. 117:2699-2707.
34. Whitworth, K., G. K. Springer, L. J. Forrester, W. G. Spollen, J. Ries, W. R. Lamberson, N. Bivens, C. N. Murphy, N. Mathialigan, J. A. Green, and R. S. Prather. 2004. Developmental expression of 2489 gene clusters during pig embryogenesis: An expressed sequence tag project. Biol. Reprod. 71:1230-1243.
35. Whitworth, K. M., C. Agca, J.-G. Kim, R. V. Patel, G. K. Springer, N. J. Bivens, L. J. Forrester, N. Mathialagan, J. A. Green, and R. S. Prather. 2005. Transcriptional profiling of pig embryogen- esis by using a 15-K member unigene set specific for pig reproductive tissues and embryos. Biol. Reprod. 72:1437-1451.
36. Wright, C. S., D. L. Becker, J. S. Lin, A. E. Warner, and K. Hardy. 2001. Stage-specific and differential expression of gap junctions in the mouse ovary: Connexin-specific roles in follicular regulation. Reproduction 121:77-88.
37. Yokoo, M., and E. Sato. 2004. Cumulus-oocyte complex interactions during oocyte maturation. Int. Rev. Cytol. 235:251-291.