Repetitive domain of Clostridium difficile toxin B exhibits cytotoxic effects on human intestinal epithelial cells and decreases epithelial barrier function

Anaerobe

Volumn 16, Issue 5, 2010, Pages 527-532

  Zemljic, Mateja ^a,c   Rupnik, Maja ^a,b   Scarpa, Melania ^c   Anderluh, Gregor ^d   Palù, Gorgio ^c   Castagliuolo, Ignazio ^c  

^a Research Department   (Slovenia)

^b UNIVERSITY OF MARIBOR   (Slovenia)

^c UNIVERSITY OF PADOVA   (Italy)

^d UNIVERSITY OF LJUBLJANA   (Slovenia)

Author keywords

Clostridium difficile; Cytotoxicity; Pathogenesis; Repetitive domain; Toxin B

Indexed keywords

CLOSTRIDIUM DIFFICILE TOXIN B; GLUCOSYLTRANSFERASE; INTERLEUKIN 8; PROTEIN ZO1;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL DAMAGE; CELL LINE; CLOSTRIDIUM DIFFICILE; CONTROLLED STUDY; CYTOKINE PRODUCTION; CYTOPATHOGENIC EFFECT; CYTOTOXICITY; ELECTRIC RESISTANCE; HUMAN; HUMAN CELL; INTESTINE EPITHELIUM CELL; NONHUMAN; PATHOPHYSIOLOGY; PERMEABILITY BARRIER; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN LOCALIZATION; RECEPTOR BINDING; STRAIN DIFFERENCE; TIGHT JUNCTION;

BACTERIAL PROTEINS; BACTERIAL TOXINS; CLOSTRIDIUM DIFFICILE; ENTEROTOXINS; EPITHELIAL CELLS; GLUCOSYLTRANSFERASES; HT29 CELLS; HUMANS; INTERLEUKIN-8; INTESTINES; PROTEIN INTERACTION DOMAINS AND MOTIFS; TIGHT JUNCTIONS;

CLOSTRIDIUM DIFFICILE;

EID: 77957019627     PISSN: 10759964     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.anaerobe.2010.06.010     Document Type: Article

References (25)

1. Lyerly D.M., Barroso L.A., Wilkins T.D., Depitre C., Corthier G. Characterization of a toxin A-negative, toxin B-positive strain of Clostridium difficile. Infect Immun 1992, 60:4633-4639.
2. Chaves-Olarte E., Freer E., Parra A., Guzman-Verri C., Moreno E., Thelestam M. R-Ras glucosylation and transient RhoA activation determine the cytophatic effect produced by toxin B variants from toxin A-negative strains of Clostridium difficile. J Biol Chem 2003, 278:7956-7963.
3. Just I., Selzer J., Wilm M., von Eichel-Streiber C., Mann M., Aktories K. Glucosylation of Rho proteins by Clostridium difficile toxin B. Nature 1995, 375:500-503.
4. Aktories K. Rho proteins: targets for bacterial toxins. Trends Microbial 1997, 5:282-288.
5. Aktories K., Just I. Monoglucosylation of low-molecular-mass GTP binding Rho proteins by clostridial cytotoxins. Trends Cell Biol 1995, 5:441-443.
6. Flegel W.A., Muller F., Daubener W., Fisher H.G., Hadding U., Northhoff H. Cytokine response by human monocytes to Clostridium difficile toxin A and toxin B. Infect Immun 1991, 59:3659-3666.
7. Kim J.M., Lee J.Y., Yoon Y.M., Oh Y.K., Youn J., Kim Y.J. NF-kappa B activation pathway is essential for the chemokine expression in intestinal cells stimulated with Clostridium difficile toxin A. Scand J Immunol 2006, 63:453-460.
8. Main E.R., Jackson S.E., Regan L. The folding and design of repeat proteins: reaching a consensus. Curr Opn Struct Biol 2003, 13:482-489.
9. Albesa-Jové D., Bertrand T., Carpenter E.P., Swain G.V., Lim J., Zhang J., et al. Four distinct structural domains in Clostridium difficile toxin B visualized using SAXS. J Mol Biol 2010, 396:1260-1270.
10. Faust C., Ye B., Song K.P. The enzymatic domain of Clostridium difficile toxin A is located within its N-terminal region. Biochem Biophys Res Commun 1998, 251:100-105.
11. Hofmann F., Busch G., Prepens U., Just I., Aktories K. Localization of the glucosyltransferase activity of Clostridium difficile toxin B to the N-terminal part of the holotoxin. J Biol Chem 1997, 272:11074-11078.
12. Frish C., Gerhard R., Aktories K., Hofmann F., Just I. The complete receptor-binding of Clostridium difficile toxin A is required for endocytosis. Biochem Biophy Res Commun 2003, 300:706-711.
13. Reinert D.J., Jank T., Aktories K., Shulz G.E. Structural basis for the function of Clostridium difficile toxin B. J Mol Biol 2005, 351:973-981.
14. Ho J.G., Greco A., Rupnik M., Ng K.K. Crystal structure of receptor- binding C-terminal repeats from Clostridium difficile toxin A. Proc Natl Acad Sci USA 2005, 102:18373-18378.
15. Greco A., Ho J.G., Lin S.J., Palcic M.M., Rupnik M., Ng K.K.-S. Carbohydrate recognition by Clostridium difficile toxin A. Nat Struct Mol Biol 2006, 13:460-461.
16. Yeh C.Y., Lin C.N., Chang C.H., Lin C.H., Lien H.T., Chen J.Y., et al. C-terminal repeats of Clostridium difficile toxin A induce production of chemokine and adhesion molecules in endothelial cells and promote migration of leukocytes. Infect Immun 2008, 76:1170-1178.
17. Castagliuolo I., Sardina M., Brun P., DeRos C., Mastrotto C., Lovato I., et al. Clostridium difficile toxin A carboxyl-terminus peptide lacking ADP-ribosyltransferase activity acts as a mucosal adjuvant. Infect Immun 2004, 72:2827-2836.
18. Pavliakova D., Moncrief J.S., Lyerly D.M., Schiffmann G., Bryla D.A., Robbins J.B., et al. Clostridium difficile recombinant toxin A repeating units as a carrier protein for conjugate vaccines: studies of pneumococcal type 14, Escherichia coli K1, and Schigella flexneri type 2a polysaccharides in mice. Infect Immun 2000, 68:2161-2166.
19. C314 and SLP-36kDa enhance the immune response toward a coadministered antigen. J Med Microbiol 2008, 57:725-731.
20. Riegler M., Sedivy R., Pothoulakis C., Hamilton G., Zacherl J., Bischof G., et al. Clostridium difficile toxin B is more potent than toxin A in damaging human colonic epithelium in vitro. J Clin Invest 1995, 95:2004-2011.
21. Qa'Dan M., Ramsey M., Daniel J., Spyres L.M., Safiejko-Mroczka B., Ortiz-Leduc W., et al. Clostridium difficile toxin B activates dual caspase-dependent and caspase-independent apoptosis in intoxicated cells. Cell Microbiol 2002, 4:425-434.
22. Savidge T.C., Pan W.H., Newman P., O'Brien M., Anton P.M., Pothoulakis C. Clostridium difficile toxin B is an inflammatory enterotoxin in human intestine. Gastroenterology 2003, 125:413-420.
23. Drudy D., Fanning S., Kyne L. Toxin A-negative, toxin B-positive Clostridium difficile. J Infect Dis 2006, 284:1-6.
24. Thelestam M., Chaves-Olarte E. Cytotoxic effects of the Clostridium difficile toxins. Curr Top Microbiol Immunol 2000, 250:85-96.
25. Steiner T.S., Flores C.A., Pizzaro T.T., Guerrant R.L. Fecal lactoferrin, interleukin1-beta, and interleukin-8 are elevated in patients with severe Clostridium difficile colitis. Clin Diagn Lab Immunol 1997, 4:719-722.