NF-κB activation pathway is essential for the chemokine expression in intestinal epithelial cells stimulated with Clostridium difficile toxin A

Scandinavian Journal of Immunology

Volumn 63, Issue 6, 2006, Pages 453-460

  Kim, J M ^a   Lee, J Y ^a   Yoon, Y M ^a   Oh, Y K ^b   Youn, J ^a   Kim, Y J ^c  

^a Hanyang University College of Medicine   (South Korea)

^b Korea University   (South Korea)

^c Joongbu University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMOKINE; CLOSTRIDIUM DIFFICILE TOXIN A; HETERODIMER; HOMODIMER; I KAPPA B ALPHA; I KAPPA B BETA; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INTERLEUKIN 8; MONOCYTE CHEMOTACTIC PROTEIN 1; PROTEIN P50; SYNAPTOTAGMIN;

ARTICLE; CELL STIMULATION; CELL STRAIN HT29; CONTROLLED STUDY; DOWN REGULATION; ENZYME ACTIVATION; ENZYME INHIBITION; ENZYME PHOSPHORYLATION; GENE TARGETING; HUMAN; HUMAN CELL; INFLAMMATION; INTESTINE EPITHELIUM CELL; ONCOGENE; PRIORITY JOURNAL; PROTEIN EXPRESSION; SIGNAL TRANSDUCTION; STIMULATION; UPREGULATION;

AMINO ACID SEQUENCE; BACTERIAL TOXINS; CHEMOKINES; CLOSTRIDIUM DIFFICILE; ENTEROTOXINS; HT29 CELLS; HUMANS; I-KAPPA B KINASE; INTESTINAL MUCOSA; MOLECULAR SEQUENCE DATA; NF-KAPPA B; PHOSPHORYLATION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION;

EID: 33745536745     PISSN: 03009475     EISSN: 13653083     Source Type: Journal    
DOI: 10.1111/j.1365-3083.2006.001756.x     Document Type: Article

References (27)

1. Kelly CP, LaMont JT. Clostridium difficile infection. Annu Rev Med 1998;49:375-90.
2. Burakoff R, Zhao L, Celifarco AJ et al. Effects of purified Clostridium difficile toxin A on rabbit distal colon. Gastroenterology 1995;109:348-54.
3. He D, Sougioultzis S, Hagen S et al. Clostridium difficile toxin A triggers human colonocyte IL-8 release via mitochondrial oxygen radical generation. Gastroenterology 2002;122:1048-57.
4. Mahida YR, Makh S, Hyde S, Gray T, Bordello SP. Effect of Clostridium difficile toxin A on human intestinal epithelial cells: induction of interleukin 8 production and apoptosis after cell detachment. Gut 1996;38:337-47.
5. Kim JM, Kim JS, Jun HC, Oh YK, Song IS, Kim CY. Differential expression and polarized secretion of CXC and CC chemokines by human intestinal epithelial cancer cell lines in response to Clostridium difficile toxin A. Microbiol Immunol 2002;46:333-42.
6. Baeuerle PA, Henkel T. Function and activation of NF-κB in the immune system. Annu Rev Immunol 1994;12:141-79.
7. Kim JM, Cho SJ, Oh YK, Jung HY, Kim YJ, Kim N. Nuclear factor-kappa B activation pathway in intestinal epithelial cells is a major regulator of chemokine gene expression and neutrophil migration induced by Bacteroides fragilis enterotoxin. Clin Exp Immunol 2002;130:59-66.
8. Elewaut D, DiDonato JA, Kim JM, Truong F, Eckmann L, Kagnoff MF. NF-kappa B is a central regulator of the intestinal epithelial cell innate immune response induced by infection with enteroinvasive bacteria. J Immunol 1999;163:1457-66.
9. Thanos D, Maniatis T. NF-κB: a lesson in family values. Cell 1995;80:529-32.
10. May MJ, Ghosh S. Signal transduction through NF-κB. Immunol Today 1998;19:80-8.
11. Jefferson KK, Smith MF Jr, Bobak DA. Roles of intracellular calcium and NF-kappa B in the Clostridium difficile toxin A-induced up-regulation and secretion of IL-8 from human monocytes. J Immunol 1999;163:5183-91.
12. Kim JM, Eckmann L, Savidge TC, Lowe DC, Witthoft T, Kagnoff MF. Apoptosis of human intestinal epithelial cells after bacterial invasion. J Clin Invest 1998;102:1815-23.
13. Kim JM, Jung HY, Lee JY, Youn J, Lee CH, Kim KH. Mitogen-activated protein kinase and activator protein-1 dependent signals are essential for Bacteroides fragiles enterotoxin-induced enteritis. Eur J Immunol 2005;35:2648-57.
14. May MJ, D'Acquisto F, Madge LA, Glockner J, Pober JS, Ghosh S. Selective inhibition of NF-kappaB activation by a peptide that blocks the interaction of NEMO with the IkappaB kinase complex. Science 2000;289:1550-4.
15. Kim JM, Oh YK, Kim YJ, Youn J, Ahn MJ. Escherichia coli up-regulates proinflammatory cytokine expression in granulocyte/macrophage lineages of CD34+ stem cells via p50 homodimeric NF-κB. Clin Exp Immunol 2004;137:341-50.
16. DiDonato JA, Mercurio F, Rosette C et al. Mapping of the inducible IκB phosphorylation sites that signal its ubiquitination and degradation. Mol Cell Biol 1996;16:1295-04.
17. Mercurio F, Zhu H, Murray BW et al. IKK-1 and IKK-2: cytokine-activated IκB kinases essential for NF-kappa B activation. Science 1997;278:860-6.
18. Cho NH, Seong SY, Huh MS, Kim NH, Choi MS, Kim IS. Induction of the gene encoding macrophage chemoattractant protein 1 by Orientia tsutsugamushi in human endothelial cells involves activation of transcription factor activator protein 1. Infect Immun 2002;70:4841-50.
19. Kim JM, Kim JS, Jung HC et al. Helicobacter pylori infection activates the NF-κB signaling pathway to induce iNOS and protect human gastric epithelial cells from apoptosis. Am J Physiol Gastrointest Liver 2003;285:G1171-80.
20. Choudhary S, Boldogh S, Garofalo R, Jamaluddin M, Brasier AR. Respiratory syncytial virus influences NF-kappaB-dependent gene expression through a novel pathway involving MAP3K14/NIK expression and nuclear complex formation with NF-kappaB2. J Virol 2005;79:8948-59.
21. Jung HC, Eckmann L, Yang SK et al. A distinct array of proinflammatory cytokines is expressed in human colon epithelial cells in response to bacterial invasion. J Clin Invest 1995;95:55-65.
22. Baldwin AS. The NF-κB and IκB proteins: new discoveries and insights. Annu Rev Immunol 1996;14:649-83.
23. DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A cytokine- responsive IκB kinase that activates the transcription factor NF-κB. Nature 1997;388:548-54.
24. Verma IM, Stevenson JK, Schwarz EM, Van Antwerp D, Miyamoto S. Rel/NF-κB/IκB family, intimate tales of association and dissociation. Genes Dev 1995;9:2723-35.
25. Mercurio F, Murray BW, Shevchenko A et al. IκB kinase (IKK) - associated protein 1, a common component of the heterogeneous IKK complex. Mol Cell Biol 1999;19:1526-38.
26. Zandi E, Chen Y, Karin M. Direct phosphorylation of IκB by IKKα and IKKβ discrimination between free and NF-κB-bound substrate. Science 1998;281:1360-3.
27. Gerhard R, Tatge H, Genth H et al. Clostridium difficile toxin A induces expression of the stress-induced early gene product RhoB. J Biol Chem 2005;280:1499-05.