Characterization of cell-banking parameters for the cryopreservation of mammalian cell lines in 100-ML cryobags

Biotechnology Progress

Volumn 26, Issue 4, 2010, Pages 1154-1163

  Heidemann, Rüdiger ^a   Lünse, Svenja ^a   Tran, Doan ^a   Zhang, Chun ^a  

^a BAYER CORPORATION   (United States)

Author keywords

Apoptosis; Cell banking; Cryobags; Mammalian cell culture; One step inoculation; Seed train expansion

Indexed keywords

APOPTOSIS; CELL BANKING; CRYOBAGS; MAMMALIAN CELLS; ONE STEP;

ANIMAL CELL CULTURE; BIOLOGICAL MATERIALS PRESERVATION; BOTTLES; CELL DEATH; EXPERIMENTS; MAMMALS; PARAMETER ESTIMATION; THAWING;

CELL CULTURE;

CRYOPROTECTIVE AGENT; DIMETHYL SULFOXIDE;

ANIMAL; ARTICLE; CELL LINE; CELL SURVIVAL; CRYOPRESERVATION; DRUG EFFECT; HAMSTER; METHODOLOGY; TEMPERATURE;

ANIMALS; CELL LINE; CELL SURVIVAL; CRICETINAE; CRYOPRESERVATION; CRYOPROTECTIVE AGENTS; DIMETHYL SULFOXIDE; TEMPERATURE;

MAMMALIA;

EID: 77956963468     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.427     Document Type: Article

References (33)

1. Aerts BGL, Ghislain YJM, Gonze MJ, Knott ISL, Magetto C. Animal-free cell culture method. U.S. Pat. US 2006/0183224 A1, 2006.
2. Al-Rubeai M, Singh PR. Apoptosis in cell culture. Curr Opin Biotechnol. 1998;9:152-156.
3. Butler M. Animal cell cultures: recent achievments and perspectives in the production of biopharmaceuticals. Appl Microbiol Biotechnol. 2005;68:283-291.
4. Chan S, Tang Y, Tao Y, Wu Y, Kelly R. Use of molecular chaperones for the enhanced production of secreted recombinant proteins in mammalian cells. U.S. Pat. US 2005/0046608 A1, 2003.
5. Chuppa S, Tsai Y-S, Yoon S, Shackleford S, Rozales C, Bhat R, Tsay G, Matanguihan C, Konstantinov K, Naveh D. Fermentor temperature as a tool for control of high-density perfusion cultures of mammalian cells. Biotechnol Bioeng. 1997;55:328-338.
6. Corsini J, Hacker C, Bare C. Serum-free cryopreservation of mammalian cell lines in either a pelleted or suspended state. Biol Proced Online. 2004;6:61-66.
7. Cullum J, Redenbach M, Hranueli D. Genetic instability. In: Flickinger MC, Drew SW, editors. Encyclopedia of bioprocess technology: Fermentation, Biocatalysis and Bioseparation. New York: John Wiley; 1999:1319-1326.
8. Fernandez-Santos MR, Esteso MC, Soler AJ, Montoro V, Garde JJ. The effects of different cryoprotectants and the temperature of addition on the survival of red deer epididymal spermatozoa. Cryo Lett. 2005;26:25-32.
9. Fieder J, Wagner N, Grammatikos S, Hoffmann H, Kaufmann H, Otto RT. Use of flow-cytometric analysis to optimize cell banking strategies for production of biopharmaceuticals from mammalian cells. J Biotechnol. 2005;120:111-120.
10. Gherna RL. Culture preservation, bacteria, fungi, yeast, and cell lines. In: Flickinger MC, Drew SW, editors. Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis and Bioseparation. New York: John Wiley; 1999:786-793.
11. Greene AE, Athreya BH, Lehr HB, Coriell LL. The effect of prolonged storage of cell cultures in dimethyl sulfoxide and glycerol prior to freezing. Cryobiology. 1970;6:552-555.
12. Grout B, Morris J, McLellan M. Cryopreservation and the maintenance of cell lines. Trends Biotechnol. 1990;8:293-297.
13. TM Kit for labeling of early apoptotic cells and discrimination between apoptotic and non-apoptotic cells. Hayward, CA: Guava Technologies, Inc. Manual; 2001.
14. Heidemann R, Mered M, Wang DQ, Gardner B, Zhang C, Michaels J, Henzler H, Abbas N, Konstantinov K. A new seed-train expansion method for recombinant mammalian cell lines. Cytotechnology. 2002;38:99-108.
15. Heidemann R, Mered M, Michaels J, Konstantinov K. Device and method for seed-train expansion of mammalian cells. U. S. Pat. 6,953,692 B2, 2005.
16. Heidemann R, Zhang C, Qi H, Rule J, Rozales C, Park S, Chuppa S, Ray M, Michaels J, Konstantinov K, Naveh D. The use of peptones as medium additives for the production of a recombinant therapeutic protein in high density perfusion cultures of mammalian cells. Cytotechnology. 2000;32:157-167.
17. Ishaque A, Thrift J, Murphy JE, Konstantinov K. Over-expression of Hsp70 in BKK-21 cells engineered to produce recombinant factor VIII promotes resistance to apoptosis and enhances secretion. Biotechnol Bioeng. 2007;97:144-155.
18. Ishaque A, Thrift J, Murphy JE, Konstantinov K. Cell surface staining of recombinant factor VIII is reduced in apoptosis resistant BHK-21 cells. J Biotechnol. 2008;137:20-27.
19. Junker B, Seeley A, Lester M, Kovatch M, Schmitt J, Boryscwicz S, Lynch J, Zhang J, Greasham R. Use of frozen bagged seed inoculum for secondary metabolite and bioconservation processes at the pilot scale. Biotechnol Bioeng. 2002;79:628-640.
20. Kaufman RJ, Wasley LC, Dormer AJ. Synthesis, processing and secretion of recombinant human factor VIII in mammalian cells. J Biol Chem. 1988;263:6352-6362.
21. 2SO, freezing density, and PEI-mediated transfection on revitalization and growth of cells, and expression of recombinant protein. Biotechnol Bioeng. 2008;100:911-922.
22. Merten OW. Safety issues of animal products used in serum-free media. Dev Biol Stand. 1999;99:167-180.
23. Ninomiya N, Shirahata S, Murakami H. Large-scale, high-density freezing of hybridomas and its application to high cell density culture. Biotechnol Bioeng. 1991;38:1110-1113.
24. Packer P, Doyle A. Culture collections. In: Flickinger MC, Drew SW, editors. Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis and Bioseparation. New York: John Wiley; 1999:766-777.
25. Regidor C, Posada M, Monteagudo D, Garaulet C, Somolinos N, Fores R, Montserrat B, Fernandez M. Stem cell transplantation- umbilical cord blood banking for unrelated transplantation: evaluation of cell separation and storage methods. Exp Hematol. 1999;27:380-385.
26. Regidor C, Vijayaraghhavan K, Basade M, He S, Gulati S. Longterm cryopreservation: successful trilineage engraftment after autologous bone marrow transplantation with bone marrow cryopreserved for seven years. J Hematology. 1998;7:185-188.
27. Reisman H. Economics. In: Flickinger MC, Drew SW, editors. Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis and Bioseparation. New York: John Wiley; 1999:863-882.
28. Schiff LJ. Review: production, characterization, and testing of banked mammalian cell substrates used to produce biological products. In Vitro Cell Dev Biol Anim. 2005;41:65-70.
29. Schmale K, Rademacher T, Fischer R, Hellwig S. Towards industrial usefulness-cryo-cell-banking of transgenic BY-2 cell cultures. J Biotechnol. 2006;124:302-311.
30. Schneider K. GMP Requirements for master and working cell bank. Pharm Ind. 2005;67:1366-1369.
31. Singh RP, Al-Rubeai M. Apoptosis. In: Flickinger MC, Drew SW, editors. Encyclopedia of bioprocess technology: Fermentation, Biocatalysis and Bioseparation. New York: John Wiley; 1999: 786-793.
32. Wang D, Gardner B, Heidemann R, Mered M, Kelsey WH. Cryopreservation bag assembly for mammalian cell lines. U.S. Pat. US 6,670,175 B2, 2003.
33. Wewetzer K, Dilmaghani K. Exposure to dimethyl sulfoxide at 37°C prior to freezing significantly improves the recovery of cryopreserved hybridoma cells. Cryobiology. 2001;43:288-292.