Electrogenic bromosulfalein transport in isolated membrane vesicles: Implementation in both animal and plant preparations for the study of flavonoid transporters

Methods in Molecular Biology

Volumn 643, Issue , 2010, Pages 307-335

  Passamonti, Sabina ^a   Tramer, Federica ^a   Petrussa, Elisa ^a   Braidot, Enrico ^a   Vianello, Angelo ^a  

^a NONE

Author keywords

bilitranslocase; Bromosulfalein; carnation petal; enzyme inactivation; enzyme kinetics; flavonoids; grape berry; membrane transport; rat kidney; rat liver

Indexed keywords

5,5' DITHIOBIS(2 NITROBENZOIC ACID); BILITRANSLOCASE; BROMSULFOPHTHALEIN; CARRIER PROTEIN; COLORING AGENT; FLAVONOID; MEMBRANE PROTEIN; MERCAPTOETHANOL; N ETHYLMALEIMIDE; POTASSIUM; VALINOMYCIN;

ANIMAL; ARTICLE; CARNATION; CELL MEMBRANE; CHEMISTRY; CYTOLOGY; DRUG EFFECT; ENZYMOLOGY; FEMALE; FLOWER; FRUIT; GRAPE; KINETICS; LIVER; METABOLISM; METHODOLOGY; MICROSOME; OSMOLARITY; PLANT; RAT; SPECTROPHOTOMETRY; TRANSPORT AT THE CELLULAR LEVEL;

ANIMALS; BIOLOGICAL TRANSPORT; CELL MEMBRANE; COLORING AGENTS; DIANTHUS; DITHIONITROBENZOIC ACID; ETHYLMALEIMIDE; FEMALE; FLAVONOIDS; FLOWERS; FRUIT; KINETICS; LIVER; MEMBRANE PROTEINS; MEMBRANE TRANSPORT PROTEINS; MERCAPTOETHANOL; MICROSOMES; OSMOLAR CONCENTRATION; PLANTS; POTASSIUM; RATS; SPECTROPHOTOMETRY; SULFOBROMOPHTHALEIN; VALINOMYCIN; VITIS;

EID: 77956943205     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60761-723-5_21     Document Type: Article

References (35)

1. Rosenthal, S., and White, E. (1925) Clinical application of the sulfobromophthalein test for hepatic function. J. Am. Med. Assoc. 84, 1112-1114.
2. Hacki, W., Bircher, J., and Preisig, R. (1976) A new look at the plasma disappearance of sulfobromophthalein (BSP): Correlation with the BSP transport maximum and the hepatic plasma flow in man. J. Lab. Clin. Med. 88, 1019-1031.
3. Scharschmidt, B. F., Waggoner, J. G., and Berk, P. D. (1975) Hepatic organic anion uptake in the rat. J. Clin. Invest. 56, 1280-1292.
4. Shibahara, S., Kitamuro, T., and Takahashi, K. (2002) Heme degradation and human disease: Diversity is the soul of life. Antioxid. Redox Signal. 4, 593-602.
5. van Montfoort, J. E., Hagenbuch, B., Groothuis, G. M., Koepsell, H., Meier, P. J., and Meijer, D. K. (2003) Drug uptake systems in liver and kidney. Curr. Drug Metab. 4, 185-211.
6. Sottocasa, G. L., Baldini, G., Sandri, G., Lunazzi, G., and Tiribelli, C. (1982) Reconstitution in vitro of sulfobromophthalein transport by bilitranslocase. Biochim. Biophys. Acta 685, 123-128.
7. Baldini, G., Passamonti, S., Lunazzi, G. C., Tiribelli, C., and Sottocasa, G. L. (1986) Cellular localization of sulfobromophthalein transport activity in rat liver. Biochim. Biophys. Acta 856, 1-10.
8. Passamonti, S., and Sottocasa, G. L. (1988) The quinoid structure is the molecular requirement for recognition of phthaleins by the organic anion carrier at the sinusoidal plasma membrane level in the liver. Biochim. Biophys. Acta 943, 119-125.
9. Karawajczyk, A., Drgan, V., Medic, N., Oboh, G., Passamonti, S., and Novic, M. (2007) Properties of flavonoids influencing the binding to bilitranslocase investigated by neural network modelling. Biochem. Pharmacol. 73, 308-320.
10. Passamonti, S., Vrhovsek, U., and Mattivi, F. (2002) The interaction of anthocyanins with bilitranslocase. Biochem. Biophys. Res. Commun. 296, 631-636.
11. Battiston, L., Passamonti, S., Macagno, A., and Sottocasa, G. L. (1998) The bilirubinbinding motif of bilitranslocase and its relation to conserved motifs in ancient biliproteins. Biochem. Biophys. Res. Commun. 247, 687-692.
12. Passamonti, S., and Sottocasa, G.L. (2002) Bilitranslocase: Structural and functional aspects of an organic anion carrier, in Recent Research Developments in Biochemistry, Pandalai, G. S., ed., Research Signpost, Kerala, pp. 371-391.
13. Passamonti, S., Cocolo, A., Braidot, E., Petrussa, E., Peresson, C., Medic, N., Macrì, F., and Vianello, A. (2005) Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase. FEBS J. 272, 3282-3296.
14. Braidot, E., Petrussa, E., Bertolini, A., Peresson, C., Ermacora, P., Loi, N., Terdoslavich, M., Passamonti, S., Macrì, F., and Vianello, A. (2008) Evidence for a putative flavonoid translocator similar to mammalian bilitranslocase in grape berries (Vitis vinifera L.) during ripening. Planta 228, 203-213.
15. Tam, J. P. (1989) High-density multiple antigen-peptide system for preparation of antipeptide antibodies. Methods Enzymol. 168, 7-15.
16. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
17. Chance, B., and Scarpa, A. (1972) Acidbase indicator for the measurement of rapid changes in hydrogen ion concentration. Methods Enzymol. 24, 336-342.
18. Passamonti, S., Battiston, L., and Sottocasa, G. L. (1998) Bilitranslocase can exist in two metastable forms with different affinities forthe substrates-evidence from cysteine and arginine modification. Eur. J. Biochem. 253, 84-90.
19. Passamonti, S., Terdoslavich, M., Margon, A., Cocolo, A., Medic, N., Micali, F., Decorti, G., and Franko, M. (2005) Uptake of bilirubin into HepG2 cells assayed by thermal lens spectroscopy. FEBS J. 272, 5522-5535.
20. Vanzo, A., Terdoslavich, M., Brandoni, A., Torres, A.M., Vrhovsek, U., and Passamonti, S. (2008) Uptake of grape anthocyanins into the rat kidney and the involvement of bilitranslocase. Mol. Nutr. Food Res. 52, 1106-1116.
21. Passamonti, S., Battiston, L., and Sottocasa, G. L. (1997) Arylsulfonylation of bilitranslocase in plasma membranes from rat liver enables to discriminate between natural and artificial substrates. Biochim. Biophys. Acta 1323, 130-136.
22. Scrutton, M. C., and Utter, M. F. (1965) Pyruvate carboxylase. V. Interaction of the enzyme with adenosine triphosphate. J. Biol. Chem. 240, 3714-3723.
23. Kitz, R., and Wilson, I. B. (1962) Esters of methanesulfonic acid as irreversible inhibitors of acetylcholinesterase. J. Biol. Chem. 237, 3245-3249.
24. Takahashi, K. (1968) The reaction of phenylglyoxal with arginine residues in proteins. J. Biol. Chem. 243, 6171-6179.
25. Antonijevic, B., and Stojiljkovic, M. P. (2007) Unequal efficacy of pyridinium oximes in acute organophosphate poisoning. Clin. Med. Res. 5, 71-82.
26. Jocelyn, P. C. (1987) Spectrophotometric assay of thiols. Methods Enzymol. 143, 44-67.
27. Eyer, P., Worek, F., Kiderlen, D., Sinko, G., Stuglin, A., Simeon-Rudolf, V., and Reiner, E. (2003) Molar absorption coefficients for the reduced Ellman reagent: Reassessment. Anal. Biochem. 312, 224-227.
28. Terrier, N., Sauvage, F. X., Ageorges, A., and Romieu, C. (2001) Changes in acidity and in proton transport at the tonoplast of grape berries during development. Planta 213, 20-28.
29. Persico, M., and Sottocasa, G. L. (1987) Measurement of sulfobromophthalein uptake in isolated rat hepatocytes by a direct spectrophotometric method. Biochim. Biophys. Acta 930, 129-134.
30. Stein, W. D. (1990) Channels, carriers, and pumps: An introduction to membrane transport. Academic Press, San Diego, USA.
31. Chance, B. (1972) Principles of differential spectrophotometry with special reference to the dual wavelength method. Methods Enzymol. 24, 322-335.
32. Schmidt, W. (2004) A high performance micro-dual-wavelength- spectrophotometer (MDWS). J. Biochem. Biophys. Methods 58, 15-24.
33. Passamonti, S., Battiston, L., and Sottocasa, G. L. (1990) Arginine residues are involved in the transport function of bilitranslocase. Biochim. Biophys. Acta 1025, 122-126.
34. Passamonti, S., and Sottocasa, G. L. (1990) The sulfhydryl groups responsible for bilitranslocase transport activity respond to the interaction of the carrier with bilirubin and functional analogues. Biochim. Biophys. Acta 1021, 9-12.
35. Passamonti, S., Battiston, L., and Sottocasa, G. L. (1999) On the mechanism of bilitranslocase transport inactivation by phenylmethylsulphonyl fluoride. Mol. Membr. Biol. 16, 167-172.