Morphometric and genetic differentiation in isolated populations of the endangered Mesoamerican stingless bee Melipona yucatanica (Hymenoptera: Apoidea) suggest the existence of a two species complex

Conservation Genetics

Volumn 11, Issue 5, 2010, Pages 2079-2084

  May Itzá, William de Jesús ^a,b   Quezada Euán, J Javier G ^a   Medina, Luis A Medina ^a   Enríquez, Eunice ^c   de la Rúa, Pilar ^b  

^a UNIVERSIDAD AUTÓNOMA DE YUCATÁN   (Mexico)

^b UNIVERSITY OF MURCIA   (Spain)

^c UNIVERSIDAD DE SAN CARLOS DE GUATEMALA   (Guatemala)

Author keywords

Conservation; DNA barcode; Melipona yucatanica; Mesoamerica; Microsatellite; Morphometrics

Indexed keywords

BEE; CONSERVATION GENETICS; ENDANGERED SPECIES; ENZYME ACTIVITY; GENE FLOW; GENETIC DIFFERENTIATION; GENETIC MARKER; HYPOTHESIS TESTING; ISOLATED POPULATION; MOLECULAR ANALYSIS; MORPHOMETRY; RARE SPECIES;

GUATEMALA [CENTRAL AMERICA]; MEXICO [NORTH AMERICA];

APOIDEA; HYMENOPTERA; MELIPONA; MELIPONINAE;

EID: 77956870794     PISSN: 15660621     EISSN: 15729737     Source Type: Journal    
DOI: 10.1007/s10592-010-0087-7     Document Type: Article

References (35)

1. Anducho-Reyes MA, Cognato AI, Hayes JL, Zúñiga G (2008) Phylogeography of the bark beetle Dendroctonus mexicanus Hopkins (Coleoptera: Curculionidae: Scolytinae). Mol Phylogenet Evol 49: 930-940.
2. Ayala R (1999) Revisión de las abejas sin aguijón de México (Hymenoptera: Apidae: Meliponini). Folia Entomol Mex 106: 1-123.
3. Bickford D, Lohman DJ, Sodhi NS, Ng PKL, Meier R, Winker K, Ingram KK, Das I (2007) Cryptic species as a window on diversity and conservation. Trends Ecol Evol 22: 148-155.
4. Brooks TM, Mittermeier RA, Mittermeier CG, da Fonseca GAB, Rylands AB, Konstant WR, Flick P, Pilgrim J, Oldfield S, Magin G, Hilton-Taylor C (2002) Habitat loss and extinction in the hotspots. Conserv Biol 16: 909-923.
5. Camargo JMF, Moure JS, Roubik DW (1988) Melipona yucatanica new species (Himenoptera: Apidae: Meliponinae): stingless bee dispersal across the Caribbean arc and post-Eucene vicariance. Pan-Pac Entomol 64: 147-157.
6. Coates AG, Obando JA (1996) The geologic evolution of the Central American Isthmus. In: Jackson JBC, Budd AF, Coates AG (eds) Evolution and environment in tropical America. Univ Chicago Press, Chicago, pp 21-56.
7. Critical Ecosystems Partnership Fund (2004) Región norte del hotspot de biodiversidad de Mesoamérica. Belice, Guatemala, México 64 pp.
8. De la Rúa P, May-Itzá W de J, Serrano J, Quezada-Euán JJG (2007) Sequence and RFLP analysis of the ITS2 ribosomal DNA in two neotropical social bees, Melipona beecheii and Melipona yucatanica (Apidae: Meliponini). Insectes Soc 54: 418-423.
9. Diniz-Filho JAF, Pignata MIB (1994) Quantitative genetics of multivariate morphometric variation in the neotropical stingless bee, Scaptotrigona postica (Hymenoptera: Meliponinae). Rev Bras Genet 17: 259-265.
10. Dorn PL, Calderon C, Melgar S, Moguel B, Solorzano E, Dumonteil E, Rodas A, De la Rúa N, Garnica R, Monroy C (2009) Two distinct Triatoma dimidiata (Latreille, 1811) taxa are found in Sympatry in Guatemala and Mexico. PLoS Negl Trop Dis 3(3): e393. doi: 10. 1371/journal. pntd. 0000393.
11. Freitas BM, Imperatriz-Fonseca VM, Medina LM, Kleinert AM, Galetto L, Nates-Parra G, Quezada-Euán JJG (2009) Diversity, threats and conservation of native bees in the neotropics. Apidologie 40: 332-346.
12. Hebert PDN, Cywinska A, Ball SL, DeWaard JR (2003) Biological identifications through DNA barcodes. Proc R Soc Lond Ser B 270: 313-321.
13. Michener CD (2007) The bees of the world, 2a edn. The Johns Hopkins University Press, Baltimore, 953 pp.
14. Morrone JJ (2006) Biogeographic areas and transition zones of Latin America and the Caribbean Islands based on panbiogeographic and cladistic analyses of the entomofauna. Annu Rev Entomol 51: 467-494.
15. Moure JM, Urban D, Melo GAR (2007) Catalogue of bees (Hymenoptera, Apoidea) in the neotropical region. Sociedade Brasileira de Entomologia, Curitiba, 1058 pp.
16. Murray TE, Kuhlmann M, Potts SG (2009) Conservation ecology of bees: populations species and communities. Apidologie 40: 211-236.
17. Paxton RJ, Weibschuh N, Quezada-Euán JJG (1999) Characterization of dinucleotide microsatellite loci for stingless bees. Mol Ecol 8: 690-692.
18. Peakall R, Smouse PE (2006) GENEALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6: 288-295.
19. Peters JM, Queller DC, Imperatriz-Fonseca VL, Roubik DW, Strassman JE (1999) Mate number, kin selection and social conflicts in stingless bees and honeybees. Proc R Soc B 266: 379-384.
20. Posada D, Crandall KA (1998) Model test: testing the model of DNA. Bioinformatics 14: 817-818.
21. Pritchard JK, Stephens M, Donnelly PJ (2000) Inference of population structure using multilocus genotype data. Genetics 155: 945-959.
22. Quezada-Euán JJG, Paxton RJ, Palmer KA, May-Itzá W de J, Tay WT, Oldroyd BP (2007) Morphological and molecular characters reveal differentiation in a neotropical social bee, Melipona beecheii (Apidae: Meliponini). Apidologie 38: 247-258.
23. Rambaut A, Drummond A (2005) Tracer. MCMC Trace File Analyser. Version 1. 3. University of Oxford, Oxford.
24. Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572-1574.
25. Rosen DE (1985) Geological hierarchies and biogeographic congruence in the Carribean. Ann Rep Mo Bot Gard 72: 636-659.
26. Rousset F (1996) Equilibrium values of measure of population subdivision for stepwise mutation processes. Genetics 142: 1357-1362.
27. Rousset F (2008) GENEPOP'007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Notes 4: 103-106.
28. Simon C, Frati F, Beckenback A, Crespi B, Liu H, Floot P (1994) Evolution, weighing and phylogenetic utility of mitochondrial DNA sequences and a compilation of conserved polymerase chain reaction primers. Ann Entomol Soc Am 87: 651-701.
29. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4. 0. Mol Biol Evol 24: 1596-1599.
30. van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4: 535-538.
31. Walsh PS, Metzqer DA, Higuchi R (1991) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10: 506-512.
32. Waugh J (2007) DNA barcoding in animal species: progress, potential and pitfalls. BioEssays 29: 188-197.
33. Weir BS, Cockerham CC (1984) Estimating F-statistics for the analysis of population structure. Evolution 38: 1358-1370.
34. Wiley EO (1981) Phylogenetics: the theory and practice of phylogenetics systematics. Wiley, New York.
35. Yurrita C, Enríquez E (2005) Distribución de abejas sin aguijón en Guatemala. San Ignacio, Chalatenango, El Salvador. CA. In Memorias del IV Seminario Mesoamericano sobre Abejas sin Aguijón, pp 130-134.