Multiplexed real-time PCR assay for discrimination of Plasmodium species with improved sensitivity for mixed infections

Journal of Clinical Microbiology

Volumn 47, Issue 4, 2009, Pages 975-980

  Shokoples, Sandra E ^a   Ndao, Momar ^b   Kowalewska Grochowska, Kinga ^c   Yanow, Stephanie K ^a  

^a PROVINCIAL LABORATORY FOR PUBLIC HEALTH   (Canada)

^b Research Institute   (Canada)

^c UNIVERSITY OF ALBERTA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; PRIMER DNA;

ARTICLE; CANADA; HUMAN; MALARIA; MICROSCOPY; MIXED INFECTION; NONHUMAN; PLASMODIUM; PLASMODIUM FALCIPARUM; PLASMODIUM MALARIAE; PLASMODIUM OVALE; PLASMODIUM VIVAX; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPECIES IDENTIFICATION; ANIMAL; BLOOD; CLASSIFICATION; COMPARATIVE STUDY; EVALUATION; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; PARASITOLOGY; POLYMERASE CHAIN REACTION;

PLASMODIUM FALCIPARUM;

ALBERTA; ANIMALS; BLOOD; DNA PRIMERS; HUMANS; MALARIA; PLASMODIUM; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 65249182510     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.01858-08     Document Type: Article

References (23)

1. Amexo, M., R. Tolhurst, G. Barnish, and I. Bates. 2004. Malaria misdiagnosis: effects on the poor and vulnerable. Lancet 364:1896-1898.
2. Anonymous. 2008. World malaria report 2008. World Health Organization, Geneva, Switzerland. http://www.who.int/malaria/wmr2008/ malaria2008.pdf.
3. Bialasiewicz, S., D. M. Whiley, M. D. Nissen, and T. P. Sloots. 2007. Impact of competitive inhibition and sequence variation upon the sensitivity of malaria PCR. J. Clin. Microbiol. 45:1621-1623.
4. Calderaro, A., G. Piccolo, F. Perandin, C. Gorrini, S. Peruzzi, C. Zuelli, L. Ricci, N. Manca, G. Dettori, C. Chezzi, and G. Snounou. 2007. Genetic polymorphisms influence Plasmodium ovale PCR detection accuracy. J. Clin. Microbiol. 45:1624-1627.
5. Cox-Singh, J., T. M. Davis, K. S. Lee, S. S. Shamsul, A. Matusop, S. Ratnam, H. A. Rahman, D. J. Conway, and B. Singh. 2008. Plasmodium knowlesi malaria in humans is widely distributed and potentially life threatening. Clin. Infect. Dis. 46:165-171.
6. de Monbrison, F., C. Angei, A. Staal, K. Kaiser, and S. Picot. 2003. Simultaneous identification of the four human Plasmodium species and quantification of Plasmodium DNA load in human blood by real-time polymerase chain reaction. Trans. R. Soc. Trop. Med. Hyg. 97:387-390.
7. Elsayed, S., K. Plewes, D. Church, B. Chow, and K. Zhang. 2006. Use of molecular beacon probes for real-time PCR detection of Plasmodium falciparum and other Plasmodium species in peripheral blood specimens. J. Clin. Microbiol. 44:622-624.
8. Erdman, L. K., and K. C. Kain. 2008. Molecular diagnostic and surveillance tools for global malaria control. Travel Med. Infect. Dis. 6:82-99.
9. Farcas, G. A., K. J. Zhong, T. Mazzulli, and K. C. Kain. 2004. Evaluation of the RealArt Malaria LC real-time PCR assay for malaria diagnosis. J. Clin. Microbiol. 42:636-638.
10. Kain, K. C., M. A. Harrington, S. Tennyson, and J. S. Keystone. 1998. Imported malaria: prospective analysis of problems in diagnosis and management. Clin. Infect. Dis. 27:142-149.
11. Kain, K. C., D. W. MacPherson, T. Kelton, J. S. Keystone, J. Mendelson, and J. D. MacLean. 2001. Malaria deaths in visitors to Canada and in Canadian travellers: a case series. Can. Med. Assoc. J. 164:654-659.
12. Mangold, K. A., R. U. Manson, E. S. Koay, L. Stephens, M. Regner, R. B. Thomson, Jr., L. R. Peterson, and K. L. Kaul. 2005. Real-time PCR for detection and identification of Plasmodium spp. J. Clin. Microbiol. 43:24352440.
13. Martens, P., and L. Hall. 2000. Malaria on the move: human population movement and malaria transmission. Emerg. Infect. Dis. 6:103-109.
14. McNabb, A., R. Chen, T. Lo, Q. Wong, and J. Isaac-Renton. 2008. Detection and speciation of Plasmodium species in blood by real-time PCR; is there a role in today's diagnostic laboratory? Can. J. Med. Lab. Sci. 70:88-98.
15. Morassin, B., R. Fabre, A. Berry, and J. F. Magnaval. 2002. One year's experience with the polymerase chain reaction as a routine method for the diagnosis of imported malaria. Am. J. Trop. Med. Hyg. 66:503-508.
16. Ndao, M., E. Bandyayera, E. Kokoskin, D. Diemert, T. W. Gyorkos, J. D. MacLean, R. St. John, and B. J. Ward. 2005. Malaria "epidemic" in Quebec: diagnosis and response to imported malaria. Can. Med. Assoc. J. 172:46-50.
17. Ndao, M., E. Bandyayera, E. Kokoskin, T. W. Gyorkos, J. D. MacLean, and B. J. Ward. 2004. Comparison of blood smear, antigen detection, and nestedPCR methods for screening refugees from regions where malaria is endemic after a malaria outbreak in Quebec, Canada. J. Clin. Microbiol. 42:26942700.
18. Perandin, F., N. Manca, A. Calderaro, G. Piccolo, L. Galati, L. Ricci, M. C. Medici, M. C. Arcangeletti, G. Snounou, G. Dettori, and C. Chezzi. 2004. Development of a real-time PCR assay for detection of Plasmodium falciparum, Plasmodium vivax, and Plasmodium ovale for routine clinical diagnosis. J. Clin. Microbiol. 42:1214-1219.
19. Rougemont, M., S. M. Van, R. Sahli, H. P. Hinrikson, J. Bille, and K. Jaton. 2004. Detection of four Plasmodium species in blood from humans by 18S rRNA gene subunit-based and species-specific real-time PCR assays. J. Clin. Microbiol. 42:5636-5643.
20. Rubio, J. M., I. Buhigas, M. Subirats, M. Baquero, S. Puente, and A. Benito. 2001. Limited level of accuracy provided by available rapid diagnosis tests for malaria enhances the need for PCR-based reference laboratories. J. Clin. Microbiol. 39:2736-2737.
21. Stauffer, W. M., M. Weinberg, R. D. Newman, L. M. Causer, M. J. Hamel, L. Slutsker, and M. S. Cetron. 2008. Pre-departure and post-arrival management of P. falciparum malaria in refugees relocating from sub-Saharan Africa to the United States. Am. J. Trop. Med. Hyg. 79:141-146.
22. Vo, T. K., P. Bigot, P. Gazin, V. Sinou, J. J. De Pina, D. C. Huynh, F. Fumoux, and D. Parzy. 2007. Evaluation of a real-time PCR assay for malaria diagnosis in patients from Vietnam and in returned travellers. Trans. R. Soc. Trop. Med. Hyg. 101:422-428.
23. Watzinger, F., M. Suda, S. Preuner, R. Baumgartinger, K. Ebner, L. Baskova, H. G. Niesters, A. Lawitschka, and T. Lion. 2004. Real-time quantitative PCR assays for detection and monitoring of pathogenic human viruses in immunosuppressed pediatric patients. J. Clin. Microbiol. 42:5189-5198.