Rapid real-time PCR genotyping of mutations associated with sulfadoxine-pyrimethamine resistance in Plasmodium falciparum

Antimicrobial Agents and Chemotherapy

Volumn 48, Issue 8, 2004, Pages 2924-2929

  Alker, Alisa P ^a   Mwapasa, Victor ^c,d   Meshnick, Steven R ^a,b  

^a IL   (United States)

^b UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^c UNIVERSITY OF MALAWI   (Malawi)

^d UNIVERSITY OF MICHIGAN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DIHYDROFOLATE REDUCTASE; DIHYDROPTEROATE SYNTHASE; DNA; FANSIDAR;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIUM CULTURE; CODON; DNA POLYMORPHISM; GENE MUTATION; GENE PROBE; GENOTYPE; MALARIA FALCIPARUM; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PLASMODIUM FALCIPARUM; POINT MUTATION; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

AFRICA; ANIMALS; ANTIMALARIALS; COMPUTER SYSTEMS; DNA PRIMERS; DNA, PROTOZOAN; DRUG COMBINATIONS; DRUG RESISTANCE; GENOTYPE; MUTATION; PLASMODIUM FALCIPARUM; POLYMORPHISM, GENETIC; PYRIMETHAMINE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SULFADOXINE; TETRAHYDROFOLATE DEHYDROGENASE;

EID: 3342910274     PISSN: 00664804     EISSN: None     Source Type: Journal    
DOI: 10.1128/AAC.48.8.2924-2929.2004     Document Type: Article

References (24)

1. Afonina, I. A., M. Reed, E. Lusby, I. G. Shishkina, and Y. S. Belousov. 2002. Minor groove binder-conjugated DNA probes for quantitative DNA detection by hybridization-triggered fluorescence. BioTechniques 32:940-949.
2. Berglez, J., P. Iliades, W. Sirawaraporn, P. Coloe, and I. Macreadie. 2004. Analysis in Escherichia coli of Plasmodium falciparum dihydropteroate synthase (DHPS) alleles implicated in resistance to sulfadoxine. Int. J. Parasitol. 34:95-100.
3. Brooks, D. R., P. Wang, M. Read, W. M. Watkins, P. F. Sims, and J. E. Hyde. 1994. Sequence variation of the hydroxymethyldihydropterin pyrophosphokinase: dihydropteroate synthase gene in lines of the human malaria parasite, Plasmodium falciparum, with differing resistance to sulfadoxine. Eur. J. Biochem. 224:397-405.
4. Chaparro, J., M. O. Rojas, and M. Wasserman. 2001. Plasmodium falciparum: underestimation of dihydrofolate reductase and dihydropteroate synthase polymorphism in field samples: a technical shortcoming of nested PCR assays with mutation-specific primers. Exp. Parasitol. 99:115-122.
5. Decuypere, S., E. Elinck, C. Van Overmeir, A. O. Talisuna, U. D'Alessandro, and J. C. Dujardin. 2003. Pathogen genotyping in polyclonal infections: application of a fluorogenic polymerase-chain-reaction assay in malaria. J. Infect. Dis. 188:1245-1249.
6. Duraisingh, M. T., J. Curtis, and D. C. Warhurst. 1998. Plasmodium falciparum: detection of polymorphisms in the dihydrofolate reductase and dihydropteroate synthetase genes by PCR and restriction digestion. Exp. Parasitol. 89:1-8.
7. Foote, S. J., D. Galatis, and A. F. Cowman. 1990. Amino acids in the dihydrofolate reductase-thymidylate synthase gene of Plasmodium falciparum involved in cycloguanil resistance differ from those involved in pyrimethamine resistance. Proc. Natl. Acad. Sci. USA 87:3014-3017.
8. García de Viedma, D., M. del Sol Díaz Infantes, F. Lasala, F. Chaves, L. Alcalá, and E. Bouza. 2002. New real-time PCR able to detect in a single tube multiple rifampin resistance mutations and high-level isoniazid resistance mutations in Mycobacterium tuberculosis. J. Clin. Microbiol. 40:988-995.
9. Jelinek, T., A. H. D. Kilian, J. Curtis, M. T. Duraisingh, G. Kabagambe, F. von Sonnenburg, and D. C. Warhurst. 1999. Plasmodium falciparum: selection of serine 108 of dihydrofolate with co-trimoxazole in Ugandan children. Am. J. Trop. Med. Hyg. 61:125-130.
10. Kearns, A. M., C. Graham, D. Burdess, J. Heatherington, and R. Freeman. 2002. Rapid real-time PCR for determination of penicillin susceptibility in pneumococcal meningitis, including culture-negative cases. J. Clin. Microbiol. 40:682-684.
11. Kublin, J. G., R. S. Witzig, A. H. Shankar, J. Q. Zurita, R. H. Gilman, J. A. Guarda, J. F. Cortese, and C. V. Plowe. 1998. Molecular assays for surveillance of antifolate-resistant malaria. Lancet 351:1629-1630.
12. Kutyavin, I., I. Afonina, A. Mills, V. Gorn, E. Lukhtanov, E. Belousov, M. Singer, D. Walburger, S. Lokhov, A. Gall, R. Dempcy, M. Reed, R. Meyer, and J. Hedgpeth. 2000. 3′-Minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures. Nucleic Acids Res. 28:655-661.
13. Livak, K. J. 1999. Allelic discrimination using fluorogenic probes and the 5′ nuclease assay. Genet. Anal. 14:143-149.
14. Mendez, F., A. Munoz, G. Carrasquilla, D. Jurado, M. Arevalo-Herrera, J. Cortese, and C. Plowe. 2002. Determinants of treatment response to sulfadoxine-pyrimethamine and subsequent transmission potential in falciparum malaria. Am. J. Epidemiol. 156:230-238,
15. Mwapasa, V., S. J. Rogerson, M. E. Molyneux, E. T. Abrams, D. D. Kamwendo, V. M. Lema, E. Tadesse, E. Chaluluka, P. E. Wilson, and S. R. Meshnick. 2004. The effect of Plasmodium falciparum malaria on peripheral and placental HIV-1 RNA concentrations in pregnant Malawian women. AIDS 18:1051-1059.
16. Nzila, A. M., E. K. Mberu, J. Sulo, H. Dayo, P. A. Winstanley, C. H. Sibley, and W. M. Watkins. 2000. Towards an understanding of the mechanism of pyrimethamine-sulfadoxine resistance in Plasmodium falciparum: genotyping of dihydrofolate reductase and dihydropteroate synthase of Kenyan parasites. Antimicrob. Agents Chemother. 44:991-996.
17. Peterson, D. S., W. K. Milhous, and T. E. Wellems. 1990. Molecular basis of differential resistance to cycloguanil and pyrimethamine in Plasmodium falciparum malaria. Proc. Natl. Acad. Sci. USA 87:3018-3022.
18. Pickard, A. L., C. Wongsrichanalai, A. Purfield, D. Kamwendo, K. Emery, C. Zalewski, F. Kawamoto, R. S. Miller, and S. R. Meshnick. 2003. Resistance to antimalarials in Southeast Asia and genetic polymorphisms in pfmdrl. Antimicrob. Agents Chemother. 47:2418-2423.
19. Plowe, C. 2001. Folate antagonists and mechanisms of resistance, p. 173-190. In P. J. Rosenthal (ed.), Antimalarial chemotherapy: mechanisms of action, resistance, and new directions in drug discovery. Humana Press, Totowa, N.Y.
20. Plowe, C., J. F. Cortese, A. Djimde, O. C. Nwanyanwu, W. M. Watkins, P. A. Winstanley, J. G. Estrada-Franco, R. E. Mollinedo, J. C. Avila, J. L. Cespedes, D. Carter, and O. K. Doumbo. 1997. Mutations in Plasmodium falciparum dihydrofolate reductase and dihydropteroate synthase and epidemiologic patterns of pyrimethamine-sulfadoxine use and resistance. J. Infect. Dis. 176:1590-1596.
21. Vet, J., A. Majithia, S. Marras, S. Tyagi, S. Dube, B. Poiesz, and F. R. Kramer. 1999. Multiplex detection of four pathogenic retroviruses using molecular beacons. Proc. Natl. Acad. Sci. USA 96:6394-6399.
22. Wang, P., C. S. Lee, R. Bayoumi, A. Djimde, O. Doumbo, G. Swedberg, L. D. Dao, H. Mshinda, M. Tanner, W. M. Watkins, P. F. Sims, and J. E. Hyde. 1997. Resistance to antifolates in Plasmodium falciparum monitored by sequence analysis of dihydropteroate synthetase and dihydrofolate reductase alleles in a large number of field samples of diverse origins. Mol. Biochem. Parasitol. 89:161-177.
23. Wang, P., M. Read, P. F. Sims, and J. E. Hyde. 1997. Sulfadoxine resistance in the human malaria parasite Plasmodium falciparum is determined by mutations in dihydropteroate synthetase and an additional factor associated with folate utilization. Mol. Microbiol. 23:979-986.
24. Wongsrichanalai, C., A. L. Pickard, W. H. Wernsdorfer, and S. R. Meshnick. 2002. Epidemiology of drug-resistant malaria. Lancet Infect. Dis. 2:209-218.