5-ketoreductase from Streptomyces bingchengensis: Overexpression and preliminary characterization

Biotechnology Letters

Volumn 32, Issue 10, 2010, Pages 1497-1502

  Wang, Xiang Jing ^a   Wang, Cheng Qin ^a   Sun, Xiao Lin ^a   Xiang, Wen Sheng ^a  

^a NORTHEAST AGRICULTURAL UNIVERSITY   (China)

Author keywords

5 Oxomilbemycins A3 and A4; Biotransformation; C5 ketoreductase (MilF); Milbemycins A3 and A4; Streptomyces bingchengensis

Indexed keywords

5-OXOMILBEMYCINS A3 AND A4; BIOTRANSFORMATION; C5-KETOREDUCTASE (MILF); MILBEMYCINS; STREPTOMYCES BINGCHENGENSIS;

ESCHERICHIA COLI;

BIOCONVERSION;

BACTERIAL DNA; BACTERIAL PROTEIN; MACROLIDE; MILBEMYCIN; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; OXIDOREDUCTASE;

ARTICLE; CHEMISTRY; COENZYME; DNA SEQUENCE; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; OXIDATION REDUCTION REACTION; STREPTOMYCES;

BACTERIAL PROTEINS; CHROMATOGRAPHY, LIQUID; CLONING, MOLECULAR; COENZYMES; DNA, BACTERIAL; ESCHERICHIA COLI; GENE EXPRESSION; MACROLIDES; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; NADP; OXIDATION-REDUCTION; OXIDOREDUCTASES; SEQUENCE ANALYSIS, DNA; STREPTOMYCES;

ESCHERICHIA COLI; STREPTOMYCES;

EID: 77956765812     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-010-0320-y     Document Type: Article

References (15)

1. De Bernardez Clark E, Schwarz EF, Rudolph R (1999) Inhibition of aggregation side reactions during in vitro protein folding. Methods Enzymol 309: 217-236.
2. He YL, Sun YH, Liu TG, Zhou XF, Bai LQ, Deng ZX (2010) Cloning of the separate meilingmycin biosynthetic gene clusters through acyltransferase-ketoreductase (AT-KR) di-domain PCR amplification. Appl Environ Microbiol. doi: 10. 1128/AEM. 02262-09.
3. Ide J, Okazaki T, Ono M, Saito A, Nakagawa K, Naito S, Sato K, Tanaka K, Yoshikawa H, Ando M, Katsumi S, Matsumoto K, Toyama T, Shibano M, Abe M (1993) Milbemycin: discovery and development. Annu Rep Sankyo Res Lab 45: 1-98.
4. Ikeda H, Omura S (1997) Avermectin biosynthesis. Chem Rev 97: 2591-2609.
5. Ikeda H, Nonomiya T, Usami M, Ohta T, Omura S (1999) Organization of the biosynthetic gene cluster for the polyketide anthelmintic macrolide avermectin in Streptomyces avermitilis. Biochemistry 96: 9509-9514.
6. Kieser T, Bibb MJ, Buttner MJ, Chater KF, Hopwood DA (2000) Practical Streptomyces genetics, 2nd edn. The John Innes Foundation, Norwich.
7. Nonaka K, Kumasaka C, Okamoto Y, Maruyama F, Yoshikawa H (1999) Bioconversion of milbemycin-related compounds: biosynthetic pathway of milbemycins. J Antibiot 52: 109-116.
8. Nonaka K, Tsukiyama T, Okamoto Y et al (2000) New milbemycins from Streptomyces hygroscopicus subsp. aureolacrimosus: fermentation, isolation and structure elucidation. J Antibiot 53: 694-704.
9. Persson B, Krook M, Jornvall H (1991) Characteristics of short chain alcohol dehydrogenases and related enzymes. Eur J Biochem 200: 537-543.
10. Sambrook J et al (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, New York.
11. Sun YH, Zhou XF, Tu GQ, Deng ZX (2003) Identification of a gene cluster encoding meilingmycin biosynthesis among multiple polyketide synthase contigs isolated from Streptomyces nanchangensis NS3226. Arch Microbiol 180: 101-107.
12. Wang XJ, Wang JD, Xiang WS (2009a) Three new milbemycin derivatives from Streptomyces bingchenggensis. J Asian Nat Prod Res 11: 597-603.
13. Wang XJ, Guo SL, Guo WQ et al (2009b) Role of nsdA in negative regulation of antibiotic production and morphological differentiation in Streptomyces bingchengensis. J Antibiot 62: 309-313.
14. Xiang WS, Wang JD, Wang XJ, Zhang J (2007) Two new β-class milbemycins from Streptomyces bingchenggensis: fermentation, isolation, structure elucidation and biological properties. J Antibiot 60(6): 351-356.
15. Xiang WS, Wang JD, Wang XJ et al (2008) New seco-milbemycins from Streptomyces bingchenggensis: fermentation, isolation and structure elucidation. J Antibiot 61: 27-32.