Large scale purification of linear plasmid DNA for efficient high throughput cloning

Biotechnology Journal

Volumn 5, Issue 9, 2010, Pages 978-985

  Noirclerc Savoye, Marjolaine ^a,b,c   Gallet, Benoit ^a,b,c   Bernaudat, Florent ^a,b,c   Vernet, Thierry ^a,b,c  

^a CEA GRENOBLE   (France)

^b INSTITUT DE BIOLOGIE STRUCTURALE   (France)

^c UNIV GRENOBLE ALPES   (France)

Author keywords

High throughput cloning; Linear vector; Membrane protein; Mistic; Mono Q

Indexed keywords

ANION EXCHANGE CHROMATOGRAPHY; DNA FRAGMENT; EFFICIENT METHOD; GENE CLONING; HIGH QUALITY; HIGH-THROUGHPUT; HIGH-THROUGHPUT CLONING; LINEAR VECTORS; MEMBRANE PROTEIN; MISTIC; PLASMID DNA;

BIOLOGICAL MEMBRANES; DNA; GENES; MICROBIOLOGY; PROTEINS; PURIFICATION; THROUGHPUT; VECTORS;

CLONING;

DNA FRAGMENT; PLASMID DNA;

ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CLONING VECTOR; DNA PURIFICATION; DNA RESTRICTION; EXPRESSION VECTOR; GENE AMPLIFICATION; HIGH THROUGHPUT SCREENING; MOLECULAR CLONING; PRIORITY JOURNAL; RESTRICTION MAPPING; SEPARATION TECHNIQUE; TECHNIQUE;

CHROMATOGRAPHY, ION EXCHANGE; CLONING, MOLECULAR; DNA FRAGMENTATION; ESCHERICHIA COLI; GENETIC VECTORS; MEMBRANE PROTEINS; PLASMIDS; RESINS, SYNTHETIC;

EID: 77956673529     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201000132     Document Type: Article

References (29)

1. Busso, D., Delagoutte-Busso, B., Moras, D., Construction of a set Gateway-based destination vectors for high-throughput cloning and expression screening in Escherichia coli. Anal. Biochem. 2005, 343, 313-321.
2. Geertsma, E. R., Poolman, B., High-throughput cloning and expression in recalcitrant bacteria. Nat. Methods 2007, 4, 705-707. (Pubitemid 47338162)
3. Qin, H., Hu, J., Hua, Y., Challa, S. V. et al., Construction of a series of vectors for high throughput cloning and expression screening of membrane proteins from Mycobacterium tuberculosis. BMC Biotechnol. 2008, 8, 51.
4. Berrow, N. S., Alderton, D., Sainsbury, S., Nettleship, J. et al., A versatile ligation-independent cloning method suitable for high-throughput expression screening applications. Nucleic Acids Res. 2007, 35, 1-12.
5. Klock, H. E., White, A., Koesema, E., Lesley, S. A., Methods and results for semi-automated cloning using integrated robotics. J. Struct. Funct. Genomics 2005, 6, 89-94. (Pubitemid 41428115)
6. Berrow, N. S., Alderton, D., Owens, R. J., The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning. Methods Mol. Biol. 2009, 498, 75-90.
7. Weeks, S. D., Drinker, M., Loll, P. J., Ligation independent cloning vectors for expression of SUMO fusions. Protein Expr. Purif. 2007, 53, 40-50.
8. Sambrook, J., Fritsch, E. F., Maniatis, T., Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor 1989.
9. Ferreira, G. N., Monteiro, G. A., Prazeres, D. M., Cabral, J. M., Downstream processing of plasmid DNA for gene therapy and DNA vaccine applications. Trends Biotechnol. 2000, 18, 380-388.
10. Heilig, J. S., Elbing, K. L., Brent, R., Large-scale preparation of plasmid DNA, in:Ausubel, F. M. et al. (Eds.), Current Protocols in Molecular Biology, Chapter 1, Unit 1.7, John Wiley & Sons, New York 2001.
11. Stadler, J., Lemmens, R., Nyhammar, T., Plasmid DNA purification. J. Gene Med. 2004, 6 Suppl 1, S54-66.
12. Levy, M. S., O'Kennedy, R. D., Ayazi-Shamlou, P., Dunnill, P., Biochemical engineering approaches to the challenges of producing pure plasmid DNA. Trends Biotechnol. 2000, 18, 296-305.
13. Chakrabarti, A., Sitaric, S., Ohi, S., A procedure for large-scale plasmid isolation without using ultracentrifugation. Biotechnol. Appl. Biochem. 1992, 16, 211-215.
14. Hines, R. N., O'Connor, K. C., Vella, G., Warren, W., Large-scale purification of plasmid DNA by anion-exchange highperformance liquid chromatography. BioTechniques 1992, 12, 430-434.
15. Diogo, M. M., Queiroz, J. A., Prazeres, D. M., Chromatography of plasmid DNA. J. Chromatogr. A 2005, 1069, 3-22.
16. Prazeres, D. M., Schluep, T., Cooney, C., Preparative purification of supercoiled plasmid DNA using anion-exchange chromatography. J. Chromatogr. A 1998, 806, 31-45. (Pubitemid 28238201)
17. Smrekar, F., Podgornik, A., Ciringer, M., Kontrec, S. et al., Preparation of pharmaceutical-grade plasmid DNA using methacrylate monolithic columns. Vaccine 28, 2039-2045.
18. Westman, E., Eriksson, S., Laas, T., Pernemalm, P. A., Skold, S. E., Separation of DNA restriction fragments by ion-exchange chromatography on FPLC columns Mono P and Mono Q. Anal. Biochem. 1987, 166, 158-171.
19. Muller, W., Fractionation of DNA restriction fragments with ion-exchangers for high-performance liquid chromatography. Eur. J. Biochem. 1986, 155, 203-212.
20. Wells, R. D., Hardies, S. C., Horn, G. T., Klein, B. et al., RPC-5 column chromatography for the isolation of DNA fragments. Methods Enzymol. 1980, 65, 327-347.
21. Tiainen, P., Galaev, I., Larsson, P. O., Plasmid adsorption to anion-exchange matrices: comments on plasmid recovery. Biotechnol. J. 2007, 2, 726-735. (Pubitemid 47062232)
22. Wallin, E., von Heijne, G., Genome-wide analysis of integral membrane proteins from eubacterial, archaean, and eukaryotic organisms. Protein Sci. 1998, 7, 1029-1038.
23. Boyd, D., Schierle, C., Beckwith, J., How many membrane proteins are there? Protein Sci. 1998, 7, 201-205. (Pubitemid 28133761)
24. Kefala, G., Kwiatkowski, W., Esquivies, L., Maslennikov, I., Choe, S., Application of Mistic to improving the expression and membrane integration of histidine kinase receptors from Escherichia coli. J. Struct. Funct. Genomics 2007, 8, 167-172.
25. Roosild, T. P., Greenwald, J., Vega, M., Castronovo, S. et al., NMR structure of Mistic, a membrane-integrating protein for membrane protein expression. Science 2005, 307, 1317-1321.
26. Roosild, T. P., Vega, M., Castronovo, S., Choe, S., Characterization of the family of Mistic homologues. BMC Struct. Biol. 2006, 6, 10.
27. Bahassi, E. M., Salmon, M. A., Van Melderen, L., Bernard, P., Couturier, M., F plasmid CcdB killer protein: ccdB gene mutants coding for non-cytotoxic proteins which retain their regulatory functions. Mol. Microbiol. 1995, 15, 1031-1037.
28. Afif, H., Allali, N., Couturier, M., Van Melderen, L., The ratio between CcdA and CcdB modulates the transcriptional repression of the ccd poison-antidote system. Mol. Microbiol. 2001, 41, 73-82. (Pubitemid 32660984)
29. Bernard, P., Couturier, M., Cell killing by the F plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes. J. Mol. Biol. 1992, 226, 735-745.