Optimization and quality assessment of the post-digestion 18O labeling based on urea for protein denaturation by HPLC/ESI-TOF mass spectrometry

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 878, Issue 22, 2010, Pages 1946-1952

  Wang, Hongbin ^a   Hu, Gaofei ^a   Zhang, Yongqian ^a   Yuan, Zheng ^a   Zhao, Xuan ^a   Zhu, Yong ^a   Cai, De ^a   Li, Yujuan ^a   Xiao, Shengyuan ^a   Deng, Yulin ^a  

^a BEIJING INSTITUTE OF TECHNOLOGY   (China)

Author keywords

18O labeling; HPLC ESI TOF; Labeling efficiency; Quantitative proteomics

Indexed keywords

ABUNDANCE RATIOS; BIOLOGICAL SAMPLES; HPLC/ESI-TOF; LABELING METHODS; MINIMAL EFFECTS; O INCORPORATION; OPTIMIZED CONDITIONS; OPTIMIZED PROTOCOL; PROTEIN DENATURATION; PROTEIN DIGESTION; PROTEIN SAMPLES; PROTEOMICS; QUALITY ASSESSMENT; QUANTITATIVE PROTEOMICS; STANDARD PROTEINS;

DENATURATION; EFFICIENCY; MASS SPECTROMETRY; MOLECULAR BIOLOGY; OPTIMIZATION; PEPTIDES; PH EFFECTS; UREA;

METABOLISM;

CYTOCHROME C; HEMOGLOBIN; MYOGLOBIN; OVALBUMIN; OXYGEN; OXYGEN 18; PEPTIDE; TRANSFERRIN; TRYPSIN; UREA; WATER; PROTEIN;

ACCURACY; ARTICLE; ELECTROSPRAY MASS SPECTROMETRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIPPOCAMPUS; HUMAN; ISOTOPE LABELING; NONHUMAN; PEPTIDE ANALYSIS; PH; PRIORITY JOURNAL; PROTEIN DENATURATION; PROTEIN STRUCTURE; PROTEOMICS; QUANTITATIVE ANALYSIS; RAT; TIME OF FLIGHT MASS SPECTROMETRY; ANIMAL; CHEMISTRY; HYDROLYSIS; METHODOLOGY;

ANIMALS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; HUMANS; HYDROLYSIS; ISOTOPE LABELING; OXYGEN ISOTOPES; PROTEIN DENATURATION; PROTEINS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; UREA;

EID: 77956667211     PISSN: 15700232     EISSN: 1873376X     Source Type: Journal    
DOI: 10.1016/j.jchromb.2010.05.030     Document Type: Article

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