Enhancement of recombinant enzyme activity in cpxA-deficient mutant of Escherichia coli

Journal of Bioscience and Bioengineering

Volumn 110, Issue 4, 2010, Pages 403-407

  Zhou, Ying ^a   Minami, Takeshi ^a   Honda, Kohsuke ^a   Omasa, Takeshi ^a   Ohtake, Hisao ^a  

^a OSAKA UNIVERSITY   (Japan)

Author keywords

Cpx system; CpxA; Recombinant enzyme; Whole cell catalysis

Indexed keywords

BACILLUS MEGATERIUM; CELL CATALYSTS; CELLULAR COMPONENTS; COMPACTIN; CPX SYSTEM; CPXA; CYTOCHROME P450; DEPENDENCY ANALYSIS; E. COLI; ENHANCING EFFECT; GALACTOSIDASES; GENES CODING; GENES ENCODING; HYDROXYLASES; INDUCIBLE PROMOTER; MUTANT ENZYMES; PARENTAL STRAINS; PSEUDONOCARDIA; REAL-TIME PCR; RECOMBINANT ENZYME; RECOMBINANT ENZYMES; SINGLE-GENE KNOCKOUT; STREPTOMYCES COELICOLOR; TRANSCRIPTIONAL LEVELS; WHOLE-CELL CATALYSIS;

BACTERIOLOGY; CATALYSIS; CATALYSTS; ENZYMES; ESCHERICHIA COLI; GENES; SUGARS;

ENZYME ACTIVITY;

BETA GALACTOSIDASE; COMPACTIN 6BETA HYDROXYLASE; CPXA PROTEIN; CYTOCHROME P450; CYTOCHROME P450 154A1; CYTOCHROME P450 BM3; OXYGENASE; PROTEIN HISTIDINE KINASE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS MEGATERIUM; ENZYME ACTIVITY; ESCHERICHIA COLI; NONHUMAN; STREPTOMYCES; STREPTOMYCES COELICOLOR; STREPTOMYCES FLAVUS;

BETA-GALACTOSIDASE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYTOCHROME P-450 ENZYME SYSTEM; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MUTATION; PROMOTER REGIONS, GENETIC; PROTEIN KINASES; RECOMBINANT PROTEINS; RNA, MESSENGER; SPECTROPHOTOMETRY, ULTRAVIOLET;

BACILLUS MEGATERIUM; ESCHERICHIA COLI; PSEUDONOCARDIA AUTOTROPHICA; STREPTOMYCES COELICOLOR; STREPTOMYCES FLAVEUS;

EID: 77956651754     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2010.04.013     Document Type: Article

References (24)

1. Schmid A., Dordick J.S., Hauer B., Kiener A., Wubbolts M., Witholt B. Industrial biocatalysis today and tomorrow. Nature 2001, 409:258-268.
2. Schoemaker H.E., Mink D., Wubbolts M.G. Dispelling the myths-biocatalysis in industrial synthesis. Science 2003, 299:1694-1697.
3. Cirino P.C., Sun L.H. Advancing biocatalysis through enzyme, cellular, and platform engineering. Biotechnol. Prog. 2008, 24:515-519.
4. Duetz W.A., Beilen J.B., Witholt B. Using proteins in their natural environment: potential and limitations of microbial whole-cell hydroxylations in applied biocatalysis. Curr. Opin. Biotechnol. 2001, 12:419-425.
5. Goldberg K., Schroer K., Lütz S., Liese A. Biocatalytic ketone reduction-a powerful tool for the production of chiral alcohols-part II: whole-cell reductions. Appl. Microbiol. Biotechnol. 2007, 76:249-255.
6. Ishige T., Honda K., Shimizu S. Whole organism biocatalysis. Curr. Opin. Chem. Biol. 2005, 9:174-180.
7. Fujii T., Fujii Y., Machida K., Ochiai A., Ito M. Efficient biotransformations using Escherichia coli with tolC acrAB mutations expressing cytochrome P450 genes. Biosci. Biotechnol. Biochem. 2009, 73:805-810.
8. Xie X., Wong W.W., Tang Y. Improving simvastatin bioconversion in Escherichia coli by deletion of bioH. Metab. Eng. 2007, 9:379-386.
9. Zhou Y., Minami T., Honda K., Omasa T., Ohtake H. Systematic screening of Escherichia coli single-gene knockout mutants for improving recombinant whole-cell biocatalysts. Appl. Microbiol. Biotechnol. 2010, 87:647-655.
10. Wulf P.D., McGuire A.M., Liu X., Lin E.C.C. Genome-wide profiling of promoter recognition by the two-component response regulator CpxR-P in Escherichia coli. J. Biol. Chem. 2002, 277:26652-26661.
11. Dorel C., Lejeune P., Rodrigue A. The Cpx system of Escherichia coli, a strategic signaling pathway for confronting adverse conditions and for settling biofilm communities?. Res. Microbiol. 2006, 157:306-314.
12. Wulf P.D., Kwon O., Lin E.C.C. The CpxRA signal transductionsystem of Escherichia coli: growth-related autoactivation and control of unanticipated target operons. J. Bacteriol. 1999, 181:6772-6778.
13. Zhou L., Lei X.H., Bochner B.R., Wanner B.L. Phenotype microarray analysis of Escherichia coli K-12 mutants with deletions of all two component systems. J. Bacteriol. 2003, 185:4956-4972.
14. Connolly L., Penas A.D.L., Alba B.M., Gross C.A. The response to extracytoplasmic stress in Escherichia coli is controlled by partially overlapping pathways. Genes Dev. 1997, 11:2012-2021.
15. Agematsu H., Matsumoto N., Fujii Y., Kabumoto H., Doi S., Machida K., Ishikawa J., Arisawa A. Hydroxylation of testosterone by bacterial cytochromes P450 using the Escherichia coli expression system. Biosci. Biotechnol. Biochem. 2006, 70:307-311.
16. Li Q.S., Schwaneberg U., Fischer F., Schmid R.D. Directed evolution of the fatty acid hydroxylase P450 BM-3 into an indole-hydroxylating catalyst. Chem. Eur. J. 2000, 6:1531-1535.
17. Sulistyaningdyah W.T., Ogawa J., Li Q.S., Maeda C., Yano Y., Schmid R.D., Shimizu S. Hydroxylation activity of P450 BM-3 mutant F87V towards aromatic compounds and its application to the synthesis of hydroquinone derivatives from phenolic compounds. Appl. Microbiol. Biotechnol. 2005, 67:556-562.
18. Baba T., Ara T., Hasegawa M., Takai Y., Okumura Y., Baba M., Datsenko K.A., Tomita M., Wanner B.L., Mori H. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2006, 2. 2006.0008.
19. Li Q.S., Schwaneberg U., Fischer M., Schmitt J., Pleiss J., Lutz-Wahl S., Schmid R.D. Rational evolution of a medium chain specific cytochrome P450 BM-3 variant. Biochem. Biophys. Acta. 2001, 114-121.
20. Ojima Y., Kawase D., Nishioka M., Taya M. Functionally undefined gene, yggE, alleviates oxidative stress generated by monoamine oxidase in recombinant Escherichia coli. Biotechnol. Lett. 2009, 31:139-145.
21. Dietrich M., Do T.A., Schmid R.D., Pleiss J., Urlacher V.B. Altering the regioselectivity of the subterminal fatty acid hydroxylase P450 BM-3 towards γ- and δ-positions. J. Bacteriol. 2009, 139:115-117.
22. Raivio T.L., Popkin D.L., Silhavy T.J. The Cpx envelope stress response is controlled by amplification and feedback inhibition. J. Bacteriol. 1999, 181:5263-5272.
23. Plate C.A., Seely S.A., Laffler T.G. Evidence for a protonmotive force related regulatory system in Escherichia coli and its effects on lactose transport. Biochemistry 1986, 25:6127-6132.
24. Mileykovskaya E., Dowhan W. The Cpx two-component signal transduction pathway is activated in Escherichia coli mutant strains lacking phosphatidylethanolamine. J. Bacteriol. 1997, 179:1029-1034.