Dentin sialophosphoprotein in biomineralization

Connective Tissue Research

Volumn 51, Issue 5, 2010, Pages 404-417

  Prasad, Monica ^a   Butler, William T ^a   Qin, Chunlin ^a  

^a BAYLOR COLLEGE OF DENTISTRY   (United States)

Author keywords

Biomineralization; Bone; Dentin; Dentin phosphoprotein; Dentin sialophosphoprotein; Dentin sialoprotein

Indexed keywords

BONE MORPHOGENETIC PROTEIN 2; CCAAT BINDING FACTOR; COMPLEMENTARY DNA; DECAPENTAPLEGIC PROTEIN; DENTIN MATRIX PROTEIN 1; DENTIN SIALOPHOSPHOPROTEIN; GELATINASE A; GLYCOPROTEIN; HYDROXYAPATITE; MATRIX METALLOPROTEINASE 20; MESSENGER RNA; SMAD1 PROTEIN; SMAD6 PROTEIN; TRANSCRIPTION FACTOR DLX5; TRANSCRIPTION FACTOR RUNX2; UNCLASSIFIED DRUG;

BIOMINERALIZATION; CELL DIFFERENTIATION; CELL MATURATION; CELLULAR DISTRIBUTION; CRYSTALLIZATION; DENTAL CARIES; DENTIN; DNA LIBRARY; DNA SEQUENCE; EXTRACELLULAR MATRIX; GEL MOBILITY SHIFT ASSAY; HUMAN; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; KNOCKOUT MOUSE; NONHUMAN; ODONTOBLAST; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN METABOLISM; PROTEIN PHOSPHORYLATION; PROTEIN STRUCTURE; RECEPTOR UPREGULATION; REGULATORY MECHANISM; REVIEW; TISSUE DISTRIBUTION; TOOTH MALFORMATION; TRANSCRIPTION REGULATION;

ANIMALS; CALCIFICATION, PHYSIOLOGIC; EXTRACELLULAR MATRIX PROTEINS; HUMANS; MICE; MUTATION; PHOSPHOPROTEINS; SIALOGLYCOPROTEINS;

EID: 77956602490     PISSN: 03008207     EISSN: 16078438     Source Type: Journal    
DOI: 10.3109/03008200903329789     Document Type: Review

References (88)

1. MacDougall, M., Simmons, D., Luan, X., Nydegger, J., Feng, J., and Gu, T.T. (1997). Dentin phosphoprotein and dentin sialoprotein are cleavage products expressed from a single transcript coded by a gene on human chromosome 4. Dentin phosphoprotein DNA sequence determination. J. Biol. Chem. 272:835-842.
2. Fisher, L.W., Torchia, D.A., Fohr, B., Young, M.F., and Fedarko, N.S. (2001). Flexible structures of SIBLING proteins, bone sialoprotein, and osteopontin. Biochem. Biophys. Res. Commun. 280:460-465.
3. Qin, C., Brunn, J.C., Cadena, E., Ridall, A., Tsujigiwa, H., Nagatsuka, H., Nagai, N., and Butler, W.T. (2002). The expression of dentin sialophosphoprotein gene in bone. J. Dent. Res. 81:392-394.
4. Baba, O., Qin, C., Brunn, J.C., Jones, J.E., Wygant, J.N., McIntyre, B.W., and Butler, W.T. (2004). Detection of dentin sialoprotein in rat periodontium. Eur. J. Oral. Sci. 112:163-170.
5. Alvares, K., Kanwar, Y.S., and Veis, A. (2006). Expression and potential role of dentin phosphophoryn (DPP) in mouse embryonic tissues involved in epithelial-mesenchymal interactions and branching morphogenesis. Dev. Dyn. 235:2980-2990.
6. Ogbureke, K.U., and Fisher, L.W. (2007). SIBLING expression patterns in duct epithelia reflect the degree of metabolic activity. J. Histochem. Cytochem. 55:403-409.
7. Xiao, S., Yu, C., Chou, X., Yuan, W., Wang, Y., Bu, L., Fu, G., Qian, M., Yang, J., Shi, Y., Hu, L., Han, B., Wang, Z., Huang, W., Liu, J., Chen, Z., Zhao, G., and Kong, X. (2001). Dentinogenesis imperfecta 1 with or without progressive hearing loss is associated with distinct mutations in DSPP. Nat. Genet. 27:201-204.
8. Zhang, X., Zhao, J., Li, C., Gao, S., Qiu, C., Liu, P., Wu, G., Qiang, B., Lo, W.H., and Shen, Y. (2001). DSPP mutation in dentinogenesis imperfecta Shields type II. Nat. Genet. 27:151-152.
9. Sreenath, T., Thyagarajan, T., Hall, B., Longenecker, G., D'Souza, R., Hong, S., Wright, J.T., MacDougall, M., Sauk, J., and Kulkarni, A.B. (2003). Dentin sialophosphoprotein knockout mouse teeth display widened predentin zone and develop defective dentin mineralization similar to human dentinogenesis imperfecta type III. J. Biol. Chem. 278:24874-24880.
10. Verdelis, K., Ling, Y., Sreenath, T., Haruyama, N., MacDougall, M., van der Meulen, M.C., Lukashova, L., Spevak, L., Kulkarni, A.B., and Boskey, A.L. (2008). DSPP effects on in vivo bone mineralization. Bone 43:983-990.
11. Veis, A., and Perry, A. (1967). The phosphoprotein of the dentin matrix. Biochemistry 6:2409-2416.
12. Butler, W.T., Bhown, M., Dimuzio, M.T., and Linde, A. (1981). Nonocollagenous proteins of dentin. Isolation and partial characterization of rat dentin proteins and proteoglycans using a three-step preparative method. Coll. Relat. Res. 1:187-199.
13. Linde, A., and Goldberg, M. (1993). Dentinogenesis. Crit. Rev. Oral. Biol. Med. 4:679-728.
14. Butler, W.T. (1995). Dentin matrix proteins and dentinogenesis. Connect. Tissue Res. 33:59-65.
15. Butler, W.T., Brunn, J.C., and Qin, C. (2003). Dentin extracellular matrix (ECM) proteins: Comparison to bone ECM and contribution to dynamics of dentinogenesis. Connect. Tissue Res. 44(Suppl. 1):171-178.
16. Ritchie, H.H., Hou, H., Veis, A., and Butler, W.T. (1994). Cloning and sequence determination of rat dentin sialoprotein, a novel dentin protein. J. Biol. Chem. 269:3698-3702.
17. Butler, W.T., Bhown, M., Brunn, J.C., D'Souza, R.N., Farach-Carson, M.C., Happonen, R.P., Schrohenloher, R.E., Seyer, J.M., Somerman, M.J., and Foster, R.A. (1992). Isolation, characterization and immunolocalization of a 53-kDal dentin sialoprotein (DSP). Matrix 12:343-351.
18. George, A., Bannon, L., Sabsay, B., Dillon, J.W., Malone, J., Veis, A., Jenkins, N.A., Gilbert, D.J., and Copeland, N.G. (1996). The carboxyl-terminal domain of phosphophoryn contains unique extended triplet amino acid repeat sequences forming ordered carboxyl-phosphate interaction ridges that may be essential in the biomineralization process. J. Biol. Chem. 271:32869-32873.
19. Ritchie, H.H., and Wang, L.H. (1996). Sequence determination of an extremely acidic rat dentin phosphoprotein. J. Biol. Chem. 271:21695-21698.
20. Feng, J.Q., Luan, X., Wallace, J., Jing, D., Ohshima, T., Kulkarni, A.B., D'Souza, R.N., Kozak, C.A., and MacDougall, M. (1998). Genomic organization, chromosomal mapping, and promoter analysis of the mouse dentin sialophosphoprotein (Dspp) gene, which codes for both dentin sialoprotein and dentin phosphoprotein. J. Biol. Chem. 273:9457-9464.
21. George, A., Srinivasan, R.S.R., Thotakura, S., and Veis, A. (1998). The phosphophoryn gene family: Identical domain structures at the carboxyl end. Eur. J. Oral. Sci. 106(Suppl. 1):221-226.
22. George, A., Srinivasan, R.S.R., Liu, K., and Veis, A. (1999). Rat dentin matrix protein 3 is a compound protein of rat dentin sialoprotein and phosphophoryn. Connect. Tissue Res. 40:49-57.
23. Ritchie, H.H., Ritchie, D.G., and Wang, L.H. (1998). Six decades of dentinogenesis research: Historical and prospective views on phosphophoryn and dentin sialoprotein. Eur. J. Oral. Sci. 106(Suppl. 1):211-220.
24. Ritchie, H.H., and Wang, L. (2000). The presence of multiple rat DSP-PP transcripts. Biochim. Biophys. Acta 1493:27-32.
25. Ritchie, H.H., Wang, L.H., and Knudtson, K. (2001). A novel rat 523 amino acid phosphophoryn: Nucleotide sequence and genomic organization. Biochim. Biophys. Acta 1520:212-222.
26. Gu, K., Chang, S., Ritchie, H.H., Clarkson, B.H., and Rutherford, R.B. (2000). Molecular cloning of a human dentin sialophosphoprotein gene. Eur. J. Oral. Sci. 108:35-42.
27. Ritchie, H.H., and Li, X. (2001). A novel rat dentin mRNA coding only for dentin sialoprotein. Eur. J. Oral. Sci. 109:342-347.
28. Yamakoshi, Y., Hu, J.C., Liu, S., Zhang, C., Oida, S., Fukae, M., and Simmer, J.P. (2003). Characterization of porcine dentin sialoprotein (DSP) and dentin sialophosphoprotein (DSPP) cDNA clones. Eur. J. Oral. Sci. 111:60-67.
29. Yamakoshi, Y., Lu, Y., Hu, J.C., Kim, J.W., Iwata, T., Kobayashi, K., Nagano, T., Yamakoshi, F., Hu, Y., Fukae, M., and Simmer, J.P. (2008). Porcine dentin sialophosphoprotein: Length polymorphisms, glycosylation, phosphorylation, and stability. J. Biol. Chem. 283:14835-14844.
30. Yuan, G., Wang, Y., Gluhak-Heinrich, J., Yang, G., Chen, L., Li, T., Wu, L.A., Chen, Z., Macdougall, M., and Chen, S. (2009). Tissue-specific expression of dentin sialophosphoprotein (DSPP) and its polymorphisms in mouse tissues. Cell Biol. Int. 33(8):816-829.
31. Chen, S., Unterbrink, A., Kadapakkam, S., Dong, J., Gu, T.T., Dickson, J., Chuang, H.H., and MacDougall, M. (2004). Regulation of the cell type-specific dentin sialophosphoprotein gene expression in mouse odontoblasts by a novel transcription repressor and an activator CCAAT-binding factor. J. Biol. Chem. 279:42182-42191.
32. Chen, S., Gu, T.T., Sreenath, T., Kulkarni, A.B., Karsenty, G., and MacDougall, M. (2002). Spatial expression of Cbfa1/ Runx2 isoforms in teeth and characterization of binding sites in the DSPP gene. Connect. Tissue Res. 43:338-344.
33. Chen, S., Rani, S., Wu, Y., Unterbrink, A., Gu, T.T., Gluhak-Heinrich, J., Chuang, H.H., and Macdougall, M. (2005). Differential regulation of dentin sialophosphoprotein expression by Runx2 during odontoblast cytodifferentiation. J. Biol. Chem. 280:29717-29727.
34. Napierala, D., Sam, K., Morello, R., Zheng, Q., Munivez, E., Shivdasani, R.A., and Lee, B. (2008). Uncoupling of chondrocyte differentiation and perichondrial mineralization underlies the skeletal dysplasia in tricho-rhino-phalangeal syndrome. Hum. Mol. Genet. 17:2244-2254.
35. Chen, S., Gluhak-Heinrich, J., Martinez, M., Li, T., Wu, Y., Chuang, H.H., Chen, L., Dong, J., Gay, I., and MacDougall, M. (2008). Bone morphogenetic protein 2 mediates dentin sialophosphoprotein expression and odontoblast differentiation via NF-Y signaling. J. Biol. Chem. 283:19359-19370.
36. Qin, C., D'Souza, R., and Feng, J.Q. (2007). Dentin matrix protein 1 (DMP1): New and important roles for biomineralization and phosphate homeostasis. J. Dent. Res. 86:1134-1141.
37. Ye, L., MacDougall, M., Zhang, S., Xie, Y., Zhang, J., Li, Z., Lu, Y., Mishina, Y., and Feng, J.Q. (2004). Deletion of dentin matrix protein-1 leads to a partial failure of maturation of predentin into dentin, hypomineralization, and expanded cavities of pulp and root canal during postnatal tooth development. J. Biol. Chem. 279:19141-19148.
38. Narayanan, K., Gajjeraman, S., Ramachandran, A., Hao, J., and George, A. (2006). Dentin matrix protein 1 regulates dentin sialophosphoprotein gene transcription during early odontoblast differentiation. J. Biol. Chem. 281:19064-19071.
39. Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H., Valentin, F., Wallace, I.M., Wilm, A., Lopez, R., Thompson, J.D., Gibson, T.J., and Higgins, D.G. (2007). ClustalW and ClustalX version 2. Bioinformatics 23:2947-2948.
40. Sun, Y., Lu, Y., Prasad, M., Wang, X., Butler, W.T., and Qin, C. (2009). Detection of full-length dentin sialophosphoprotein in rat dentin-pulp complex. J. Dent. Res. 88 (Special Issue): Abstract # 1690.
41. Butler, W.T., Bhown, M., DiMuzio, M.T., Cothran, W.C., and Linde, A. (1983). Multiple forms of rat dentin phosphoproteins. Arch. Biochem. Biophys. 225:178-186.
42. Huq, N.L., Cross, K.J., Talbo, G.H., Riley, P.F., Loganathan, A., Crossley, M.A., Perich, J.W., and Reynolds, E.C. (2000). N-terminal sequence analysis of bovine dentin phosphophoryn after conversion of phosphoseryl to S-propylcysteinyl residues. J. Dent. Res. 79:1914-1919.
43. Huq, N.L., Loganathan, A., Cross, K.J., Chen, Y.Y., Johnson, N.I., Willetts, M., Veith, P.D., and Reynolds, E.C. (2005). Association of bovine dentine phosphophoryn with collagen fragments. Arch. Oral. Biol. 50:807-819.
44. Dickson, I.R., Dimuzio, M.T., Volpin, D., Ananthanarayanan, S., and Veis, A. (1975). The extraction of phosphoproteins from bovine dentin. Calcif. Tissue Res. 19:51-61.
45. Dimuzio, M.T., and Veis, A. (1978). The biosynthesis of phosphophoryns and dentin collagen in the continuously erupting rat incisor. J. Biol. Chem. 253:6845-6852.
46. MacDougall, M., Zeichner-David, M., and Slavkin, H.C. (1985). Production and characterization of antibodies against murine dentine phosphoprotein. Biochem. J. 232:493-500.
47. Lee, S.L., Veis, A., and Glonek, T. (1977). Dentin phosphoprotein: An extracellular calcium-binding protein. Biochemistry 16:2971-2979.
48. Jonsson, M., and Fredriksson, S. (1978). Isoelectric focusing of the phosphoprotein of rat-incisor dentin in ampholine and acid pH gradients. Evidence for carrier ampholyte-protein complexes. J. Chromatogr. 157:234-242.
49. Qin, C., Brunn, J.C., Baba, O., Wygant, J.N., McIntyre, B.W., and Butler, W.T. (2003). Dentin sialoprotein isoforms: Detection and characterization of a high molecular weight dentin sialoprotein. Eur. J. Oral. Sci. 111:235-242.
50. Yamakoshi, Y., Hu, J.C., Fukae, M., Iwata, T., Kim, J.W., Zhang, H., and Simmer, J.P. (2005). Porcine dentin sialoprotein is a proteoglycan with glycosaminoglycan chains containing chondroitin 6-sulfate. J. Biol. Chem. 280:1552-1560.
51. Sugars, R.V., Olsson, M.L., Waddington, R., and Wendel, M. (2006). Substitution of bovine dentine sialoprotein with chondroitin sulfate glycosaminoglycan chains. Eur. J. Oral. Sci. 114:89-92.
52. Yamakoshi, Y., Hu, J.C., Fukae, M., Zhang, H., and Simmer, J.P. (2005). Dentin glycoprotein: The protein in the middle of the dentin sialophosphoprotein chimera. J. Biol. Chem. 280:17472-17479.
53. Qin, C., Cook, R.G., Orkiszewski, R.S., and Butler, W.T. (2001). Identification and characterization of the carboxylterminal region of rat dentin sialoprotein. J. Biol. Chem. 276:904-909.
54. Qin, C., Baba, O., and Butler, W.T. (2004). Post-translational modifications of sibling proteins and their roles in osteogenesis and dentinogenesis. Crit. Rev. Oral. Biol. Med. 15:126-136.
55. Yamakoshi, Y., Hu, J.C., Iwata, T., Kobayashi, K., Fukae, M., and Simmer, J.P. (2006). Dentin sialophosphoprotein is processed by MMP-2 and MMP-20 in vitro and in vivo. J. Biol. Chem. 281:38235-38243.
56. Steiglitz, B.M., Ayala, M., Narayanan, K., George, A., and Greenspan, D.S. (2004). Bone morphogenetic protein-1/ Tolloid-like proteinases process dentin matrix protein-1. J. Biol. Chem. 279:980-986.
57. Butler, W.T. (1998). Dentin matrix proteins. Eur. J. Oral. Sci. 106(Suppl. 1):204-210.
58. Qin, C., Brunn, J.C., Cadena, E., Ridall, A., and Butler, W.T. (2003). Dentin sialoprotein in bone and dentin sialophosphoprotein gene expressed by osteoblasts. Connect. Tissue Res. 44(Suppl. 1):179-183.
59. Ogbureke, K.U., and Fisher, L.W. (2004). Expression of SIBLINGs and their partner MMPs in salivary glands. J. Dent. Res. 83:664-670.
60. Weinstock, M., and Leblond, C.P. (1973). Radioautographic visualization of the deposition of a phosphoprotein at the mineralization front in the dentin of the rat incisor. J. Cell Biol. 56:838-845.
61. Rabie, A.M., and Veis, A. (1995). An immunocytochemical study of the routes of secretion of collagen and phosphophoryn from odontoblasts into dentin. Connect. Tissue Res. 31:197-209.
62. Huang, B., Sun, Y., Maciejewska, I., Qin, D., Peng, T., McIntyre, B., Wygant, J., Butler, W.T., and Qin, C. (2008). Distribution of SIBLING proteins in the organic and inorganic phases of rat dentin and bone. Eur. J. Oral. Sci. 116:104-112.
63. D'Souza, R.N., Bronckers, A.L., Happonen, R.P., Doga, D.A., Farach-Carson, M.C., and Butler, W.T. (1992). Developmental expression of a 53 KD dentin sialoprotein in rat tooth organs. J. Histochem. Cytochem. 40:359-366.
64. Bronckers, A.L., D'Souza, R.N., Butler, W.T., Lyaruu, D.M., van Dijk, S., Gay, S., and Woltgens, J.H. (1993). Dentin sialoprotein: Biosynthesis and developmental appearance in rat tooth germs in comparison with amelogenins, osteocalcin and collagen type-I. Cell Tissue Res. 272:237-247.
65. Baba, O., Qin, C., Brunn, J.C., Wygant, J.N., McIntyre, B.W., and Butler, W.T. (2004). Colocalization of dentin matrix protein 1 and dentin sialoprotein at late stages of rat molar development. Matrix Biol. 23:371-379.
66. Qin, C., Baba, O., Brunn, J.C., McKee, M.D., Bonewald, L., and Butler, W.T. (2005). Dentin matrix protein 1 (DMP1) and dentin sialophosphoprotein (DSPP) share unique properties including tissue localization, proteolytic processing, and high molecular weight forms. In Proceedings of the 8th ICCBMT, J. Sodek and W. Landis (eds.) pp. 174-177. Banff, Alberta, Canada: University of Toronto Press.
67. Begue-Kirn, C., Ruch, J.V., Ridall, A.L., and Butler, W.T. (1998). Comparative analysis of mouse DSP and DPP expression in odontoblasts, preameloblasts, and experimentally indu ced odontoblast-like cells. Eur. J. Oral. Sci. 106(Suppl. 1):254-259.
68. Begue-Kirn, C., Krebsbach, P.H., Bartlett, J.D., and Butler, W.T. (1998). Dentin sialoprotein, dentin phosphoprotein, enamelysin and ameloblastin: Tooth-specific molecules that are distinctively expressed during murine dental differentiation. Eur. J. Oral. Sci. 106:963-970.
69. Paine, M.L., Luo, W., Wang, H.J., Bringas, P., Jr., Ngan, A.Y., Miklus, V.G., Zhu, D.H., MacDougall, M., White, S.N., and Snead, M.L. (2005). Dentin Sialoprotein and dentin phosphoprotein overexpression during amelogenesis. J. Biol. Chem. 280:31991-31998.
70. Holappa, H., Nieminen, P., Tolva, L., Lukinmaa, P.L., and Alaluusua, S. (2006). Splicing site mutations in dentin sialophosphoprotein causing dentinogenesis imperfecta type II. Eur. J. Oral. Sci. 114:381-384.
71. Malmgren, B., Lindskog, S., Elgadi, A., and Norgren, S. (2004). Clinical, histopathologic, and genetic investigation in two large families with dentinogenesis imperfecta type II. Hum. Genet. 114:491-498.
72. Rajpar, M.H., Koch, M.J., Davies, R.M., Mellody, K.T., Kielty, C.M., and Dixon, M.J. (2002). Mutation of the signal peptide region of the bicistronic gene DSPP affects translocation to the endoplasmic reticulum and results in defective dentine biomineralization. Hum. Mol. Genet. 11:2559-2565.
73. Kim, J.W., Nam, S.H., Jang, K.T., Lee, S.H., Kim, C.C., Hahn, S.H., Hu, J.C., and Simmer, J.P. (2004). A novel splice acceptor mutation in the DSPP gene causing dentinogenesis imperfecta type II. Hum. Genet. 115:248-254.
74. Dong, J., Gu, T., Jeffords, L., and MacDougall, M. (2005). Dentin phosphoprotein compound mutation in dentin sialophosphoprotein causes dentinogenesis imperfecta type III. Am. J. Med. Genet. 132A:305-309.
75. Zanetti, M., de Bernard, B., Jontell, M., and Linde, A. (1981). Ca2+-binding studies of the phosphoprotein from rat-incisor dentine. Eur. J. Biochem. 113:541-545.
76. Marsh, M.E. (1989). Binding of calcium and phosphate ions to dentin phosphophoryn. Biochemistry 28:346-352.
77. Kuboki, Y., Fujisawa, R., Aoyama, K., and Sasaki, S. (1979). Calcium-specific precipitation of dentin phosphoprotein: A new method of purification and the significance for the mechanism of calcification. J. Dent. Res. 58:1926-1932.
78. Marsh, M.E. (1989). Self-association of calcium and magnesium complexes of dentin phosphophoryn. Biochemistry 28:339-345.
79. Linde, A. (1989). Dentin matrix proteins: Composition and possible functions in calcification. Anat. Rec. 224:154-166.
80. Boskey, A.L., Maresca, M., Doty, S., Sabsay, B., and Veis, A. (1990). Concentration-dependent effects of dentin phosphophoryn in the regulation of in vitro hydroxyapatite formation and growth. Bone Miner. 11:55-65.
81. Saito, T., Arsenault, A.L., Yamauchi, M., Kuboki, Y., and Crenshaw, M.A. (1997). Mineral induction by immobilized phosphoproteins. Bone 21:305-311.
82. Stetler-Stevenson, W.G., and Veis, A. (1986). Type I collagen shows a specific binding affinity for bovine dentin phosphophoryn. Calcif. Tissue Int. 38:135-141.
83. Traub, W., Jodaikin, A., Arad, T., Veis, A., and Sabsay, B. (1992). Dentin phosphophoryn binding to collagen fibrils. Matrix 12:197-201.
84. Saito, T., Ogawa, M., Hata, Y., and Bessho, K. (2004). Acceleration effect of human recombinant bone morphogenetic protein-2 on differentiation of human pulp cells into odontoblasts. J. Endod. 30:205-208.
85. Jadlowiec, J.A., Zhang, X., Li, J., Campbell, P.G., and Sfeir, C. (2006). Extracellular matrix-mediated signaling by dentin phosphophoryn involves activation of the Smad pathway independent of bone morphogenetic protein. J. Biol. Chem. 281:5341-5347.
86. Wu, L., Zhu, F., Wu, Y., Lin, Y., Nie, X., Jing, W., Qiao, J., Liu, L., Tang, W., Zheng, X., and Tian, W. (2008). Dentin sialophosphoprotein-promoted mineralization and expression of odontogenic genes in adipose-derived stromal cells. Cells Tissues Organs 187:103-112.
87. Boskey, A., Spevak, L., Tan, M., Doty, S.B., and Butler, W.T. (2000). Dentin sialoprotein (DSP) has limited effects on in vitro apatite formation and growth. Calcif. Tissue Int. 67:472-478.
88. Suzuki, S., Sreenath, T., Haruyama, N., Honeycutt, C., Terse, A., Cho, A., Kohler, T., Muller, R., Goldberg, M., and Kulkarni, A.B. (2009). Dentin sialoprotein and dentin phosphoprotein have distinct roles in dentin mineralization. Matrix Biol. 28:221-229.