Number and nuclear morphology of TH+ and TH- neurons in the mouse ventral midbrain using epifluorescence stereology

Experimental Neurology

Volumn 225, Issue 2, 2010, Pages 328-340

  Prasad, Kavita ^a,b,c   Richfield, Eric K ^a,b,c  

^a Department of Pathology and Lab Medicine ^*  (United States)

^b Molecular Histology Center   (United States)

^c RUTGERS UNIVERSITY   (United States)

Author keywords

DAPI; Dopamine; Flourescence; Mouse; NeuN; Stereology; Substantia nigra; Tyrosine hydroxylase; Ventral tegmental area

Indexed keywords

CELL NUCLEUS ANTIGEN; TYROSINE 3 MONOOXYGENASE;

ACCURACY; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BRAIN REGION; CELL COUNT; CELL NUCLEUS; CELL STRUCTURE; CELL TYPE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CYTOPLASM; DOPAMINERGIC NERVE CELL; FLUORESCENCE; INTERMETHOD COMPARISON; MALE; MESENCEPHALON; MOUSE; NERVE CELL; NONHUMAN; PRIORITY JOURNAL; RAT; STEREOLOGY; ANALYSIS OF VARIANCE; ANIMAL; CYTOLOGY; FLUORESCENCE MICROSCOPY; METABOLISM; NUCLEAR SHAPE; PHYSIOLOGY; PROCEDURES;

ANALYSIS OF VARIANCE; ANIMALS; CELL COUNT; CELL NUCLEUS SHAPE; MESENCEPHALON; MICE; MICROSCOPY, FLUORESCENCE; NEURONS; TYROSINE 3-MONOOXYGENASE;

EID: 77956493724     PISSN: 00144886     EISSN: 10902430     Source Type: Journal    
DOI: 10.1016/j.expneurol.2010.07.004     Document Type: Article

References (50)

1. Baquet Z.C., Bickford P.C., et al. Brain-derived neurotrophic factor is required for the establishment of the proper number of dopaminergic neurons in the substantia nigra pars compacta. J. Neurosci. 2005, 25(26):6251-6259.
2. Baquet Z.C., Williams D., et al. A comparison of model-based (2D) and design-based (3D) stereological methods for estimating cell number in the substantia nigra pars compacta of the C57BL/6J Mouse. Neuroscience 2009, 161:1082-1090.
3. Beil M., Irinopoulou T., et al. Application of confocal scanning laser microscopy for an automated nuclear grading of prostate lesions in three dimensions. J. Microsc. 1996, 183:231-240.
4. Boffa D.J., Waka J., et al. Measurement of apoptosis of intact human islets by confocal optical sectioning and stereologic analysis of YO-PRO-1-stained islets. Transplantation 2005, 79:842-845.
5. Bowyer F.J., Tank A.W., et al. The influence of environmental termperaure on the transient effects of methamphphetamine on dopamine levels and dopamine relese in rat straitum. JPET 1992, 260:817-824.
6. Brændgaard H., Gundersen H.J.G. The impact of recent stereological advances on quantitative studies of the nervous system. J. Neurosci. Meth. 1986, 18(1-2):39-78.
7. Cannon J.R., Greenamyre J.T. NeuN is not a reliable marker of dopamine neurons in rat substantia nigra. Neurosci. Lett. 2009, 464(1):14-17.
8. Cho J.Y., Min N., et al. Rapid down-regulation of tyrosine hydroxylase expression in the olfactory bulb of naris-occluded adult rats. J. Comp. Neurol. 1998, 369:264-276.
9. Coban A., Filipov N.M. Dopaminergic toxicity associated with oral exposure to the herbicide atrazine in juvenile male C57BL/6 mice. J. Neurochem. 2007, 100(5):1177-1187.
10. Dorph-Petersen K.-A., Nyengaard J.R., et al. Tissue shrinkage and unbiased stereological estimation of particle number and size. J. Microsc. 2001, 204(3):232-246.
11. Gardella D., Hatton W.J., et al. Differential tissue shrinkage and compression in the z-axis: implications for optical disector counting in vibratome-, plastic- and cryosections. J. Neurosci. Meth. 2003, 124(1):45-59.
12. Georgievska B., Kirik D., et al. Aberrant sprouting and downregulation of tyrosine hydroxylase in lesioned nigrostriatal dopamine neurons induced by long-lasting overexpression of glial cell line derived neurotroophic factor in the striatum by lentiviral gene transfer. Exp. Neurol. 2002, 177:461-474.
13. German D.C., Nelson E.L., et al. The neurotoxin MPTP causes degeneration of specific nucleus A8, A9 and A10 dopaminergic neurons in the mouse. Neurodegeneration 1996, 5(4):299-312.
14. Giannakopoulos P., Herrmann F.R., et al. Tangle and neuron numbers, but not amyloid load, predict cognitive status in Alzheimer's disease. Neurology 2003, 60(9):1495-1500.
15. Gittins R., Harrison P.J. Neuronal density, size and shape in the human anterior cingulate cortex: a comparison of Nissl and NeuN staining. Brain Res. Bull. 2004, 63(2):155-160.
16. Glaser J., Greene G., et al. Stereology for biological research: with a focus on neuroscience 2007, VT, MBF Press, Williston.
17. Gray J.D., Kolesik P., et al. Confocal measurement of the three-dimensional size and shape of plant parenchyma cells in a developing fruit tissue. Plant J. 1999, 19:229-236.
18. Gundersen H.J.G. Stereology: the fast lane between neuroanatomy and brain function-or still only a tightrope?. Acta Neurol. Scand. 1992, 85(S137):8-13.
19. Gundersen H.J.G., Bagger P., et al. The new stereological tools: disector, fractionator, nucleator and point sampled intercepts and their use in pathological research and diagnosis. APMIS 1988, 96(7-12):857-881.
20. Gundersen H.J.G., Jensen E.B.V., et al. The efficiency of systematic sampling in stereology reconsidered. J. Microsc. 1999, 193(3):199-211.
21. Hart A.M., Terenghi G. Frozen-section fluorescence microscopy and stereology in the quantification of neuronal death within dorsal root ganglia. J. Mol. Histol. 2004, 35(6):565-580.
22. Howell K., Hopkins N., et al. Combined confocal microscopy and stereology: a highly efficient and unbiased approach to quantitative structural measurement in tissues. Exp. Physiol. 2002, 87:747-756.
23. Kastner A., Herrero M.T., et al. Decreased tyrosine hydroxylase content in the dopaminergic neurons of MPTP-intoxicated monkeys: effect of levodopa and GM1 ganglioside therapy. Ann. Neurol. 1994, 36:206-214.
24. Kharlanova N.G., Lomov Y.M., et al. Ultrastructural peculiarities of intranuclear inclusions in some target cells during endotoxin shock. Bull. Exp. Biol. Med. 2002, 133(5):510-514.
25. Kogan F.J., Nichols W.K., et al. Influence of methamphetamine on nigral and striatal tyrosine hydroxylase activity and on striatal dopamine levels. Eur. J. Pharmacol. 1976, 36:363-371.
26. Kubinova L. Advantages of stereological methods in biomedicine. Efficiently obtaining unbiased estimates of geometrical characteristics for 3-D structures. IEEE Eng. Med. Biol. Mag. 1998, 17(2):110-115.
27. Kubinova L., Janacek J. Confocal microscopy and stereology: estimating volume, number, surface area and length by virtual test probes applied to three-dimensional images. Microsc. Res. Tech. 2001, 53:425-435.
28. Kubinova L., Janacek J., et al. Confocal stereology and image analysis: methods for estimating geometrical characteristics of cells and tissues from three-dimensional confocal images. Physiol. Res. 2004, 53:S47-S55.
29. Kubinova L., Janacek J., et al. Three-dimensional study of the capillary supply of skeletal muscle fibers using confocal microscopy 2001, 22:217-227.
30. Lafarga M., Berciano M.T., et al. Nucleolar organization in granule cell neurons of the rat cerebellum. J. Neurocytol. 1989, 18(1):19-26.
31. Lind D., Franken S., et al. Characterization of the neuronal marker NeuN as a multiply phosphorylated antigen with discrete subcellular localization. J. Neurosci. Res. 2005, 79(3):295-302.
32. Nair-Roberts R.G., Chatelain-Badie S.D., et al. Stereological estimates of dopaminergic, GABAergic and glutamatergic neurons in the ventral tegmental area, substantia nigra and retrorubral field in the rat. Neuroscience 2008, 152(4):1024-1031.
33. Nelson E.L., Liang C.L., et al. Midbrain dopaminergic neurons in the mouse: computer-assisted mapping. J. Comp. Neurol. 1996, 369(3):361-371.
34. Nishi K., Kondo T., et al. Difference in recovery patterns of striatal dopamine content, tyrosine hydroxylase activity, and total biopterin content after 1-methyl-4-phenyl-1, 2, 3, 6-tetrahyroperidine administration: a comparison of young and older mice. Brain Res. 1989, 489:157-162.
35. Peterson D.A. Quantitative histology using confocal microscopy: implementation of unbiased stereology procedures. Methods: A Companion to Methods in Enzymology 1999, 18:493-507.
36. Peterson D.A. The use of fluoresent probes in cell-counting procedures. quantitative methods in neuroscience: a neuroanatomical approach 2004, 86-115. Oxford University Press, Oxford. S.M. Evans, A.M. Janson, J.R. Nyengaard (Eds.).
37. Peterson D.A., Suppl The use of multiple fluorescence labels for determination of cell number phenotype by design-based stereology. 2004, 10:728-729.
38. Pioli E.Y., Dovero S., et al. Asymmetrically lesioned mesencephalon in healthy rodents: call for caution. Brain Res. 2004, 1022:251-253.
39. Prasad K., Richfield E.K. Sporadic midbrain dopamine neuron abnormalities in laboratory mice. Neurobiol. Dis. 2008, 32(2):262-272.
40. Reed M.G., Howard C.V., et al. Confocal imaging and second-order stereological analysis of a liquid foam. J. Microsc. 1997, 185:313-320.
41. Schmitz C., Hof P.R. Design-based stereology in neuroscience. Neuroscience 2005, 130(4):813-831.
42. Sherer, T. and K. Carlson (2010). Protocol Issue #1: Histological and biochemical methods. Pre-Clinical Research Models.
43. Simon J.R., Richter J.A., et al. Age-dependent alterations in dopamine content, tyrosine hydroxylase activity, and dopamine uptake in the striatum of the Weaver mutant mouse. J. Neurochem. 1994, 62:543-548.
44. Slomianka L., West M.J. Estimators of the precision of stereological estimates: an example based on the CA1 pyramidal cell layer of rats. Neuroscience 2005, 136(3):757-767.
45. West M.J. New stereological methods for counting neurons. Neurobiol. Aging 1993, 14(4):275-285.
46. West M.J. Design-based stereological methods for counting neurons. Prog. Brain Res. 2002, 135:43-51.
47. West M.J., L. Slomianka, et al.: Unbiased stereological estimation of the total number of neurons in the subdivisions of the rat hippocampus using the optical fractionator.
48. White F.A., Keller-Peck C.R., et al. Widespread elimination of naturally occurring neuronal death in bax-deficient mice. J. Neurosci. 1998, 18(4):1428-1439.
49. Wong M., Wuethrich P., et al. Zone-specific cell biosynthetic activity in mature bovine articular cartilage: a new method using confocal microscope stereology and quantitative autoradiography. J. Orthop. Res. 1996, 14:424-432.
50. Zaborsky L., Vadasz C. The midbrain dopaminergic system: anatomy and genetic variation in dopamine neuron number of inbred mouse strains. 2001, 31:47-59.