Zone-specific cell biosynthetic activity in mature bovine articular cartilage: A new method using confocal microscopic stereology and quantitative autoradiography

Journal of Orthopaedic Research

Volumn 14, Issue 3, 1996, Pages 424-432

  Wong, M ^a   Wuethrich, P ^a   Eggli, P ^a   Hunziker, E ^a  

^a M E Mueller Inst for Biomechanics   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

BIOSYNTHESIS; CARTILAGE; CYTOLOGY; DISEASES; EMULSIONS; METABOLISM; OPTICAL MICROSCOPY; RADIOGRAPHY; TISSUE;

CELL VOLUME FRACTION; CHONDROCYTE; CONFOCAL MICROSCOPIC STEREOLOGY; GRAINS PER CELL; MATURE BOVINE ARTICULAR CARTILAGE; MEAN CELL SURFACE; MEAN CELL VOLUME; QUANTITATIVE AUTORADIOGRAPHY; SURFACE DENSITY; ZONE SPECIFIC CELL BIOSYNTHETIC ACTIVITY;

CELLS;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; ARTICULAR CARTILAGE; AUTORADIOGRAPHY; BIOSYNTHESIS; CARTILAGE CELL; CARTILAGE MATRIX; CATTLE; CELL DENSITY; CELL SHAPE; CELL SURFACE; CELL VOLUME; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; MORPHOMETRICS; NONHUMAN; PRIORITY JOURNAL; STEREOLOGY;

EID: 0030152594     PISSN: 07360266     EISSN: None     Source Type: Journal    
DOI: 10.1002/jor.1100140313     Document Type: Article

References (42)

1. 1 Aydelotte MB, Greenhill RR, Kuettner KE: Differences between sub‐populations of cultured bovine articular chondrocytes. II. Proteoglycan metabolism. Connect Tissue Res 18: 223–234, 1988.
2. 2 Aydelotte MB, Schmid TM, Greenhill RR. Luchene L. Schumacher BL, Kuettner KE: Synthesis of collagen by cultured bovine chondrocytes derived from different depths of articular cartilage, Trans Orthop Res Soc 16: 26, 1991
3. 3 Baddeley AJ, Gundersen HJG, Cruz‐Orive LM: Estimation of surface area from vertical sections. J Microsc 142: 259–276, 1986.
4. 4 Bayliss M, Venn M, Maroudas A, Ali SY: Structure of proteoglycans from different layers of human articular cartilage. Biochem J 209: 387–400, 1983
5. 5 Benya PD, Shaffer JD: Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels. Cell 30: 215–224, 1982
6. 6 Burton‐Wurster N, Vernier‐Singer M, Farquhar T, Lust G: Effect of compressive loading and unloading on the synthesis of total protein, proteoglycan, and fibronectin by canine cartilage explants. J Orthop Res 11: 717–729, 1993
7. 7 Buschmann M, Maurer A, Berger E, Hunziker E: A method of quantitative autoradiography for the spatial localization of proteoglycan synthesis rates in cartilage. J Histochem Cytochem 44: 423–431, 1996
8. 8 Caterson B, Lowther DA: Changes in the metabolism of the proteoglycans from sheep articular cartilage in response to mechanical stress. Biochim Biophys Acta 540: 412–122, 1978
9. 9 Cruz‐Orive LM, Hunziker EB: Stereology for anisotropiccells: application to growth cartilage. J Microsc 143: 47–80, 1986
10. 10 Eggli PS, Hunziker EB Schenk RK: Quantitation of structural features characterizing weight‐ and less‐weight‐bearing regions in articular cartilage: a stereological analysis of medial femoral condyles in young adult rabbits. Anat Rec 222: 217–227, 1988
11. 11 Gray ML, Pizzanelli AM, Grodzinsky AJ, Lee RC: Mechanical and physicochemical determinants of the chondrocyte biosynthetic response. J Orthop Res 6: 777–792, 1988
12. 12 Guilak F: Volume and surface area measurement of viable chondrocytes in situ using geometric modelling of serial confocal sections. J Microsc 173: 245–256, 1994
13. 13 Guilak F, Ratcliffe A, Mow VC: Chondrocyte deformation and local tissue strain in articular cartilage: a confocal microscopy study. J Orthop Res 13: 410–421, 1995
14. 14 Gundersen HJ, Bagger P, Bendtsen TF, Evans SM, Korbo L, Marcussen N, Moller A, Nielsen K, Nyengaard JR, Pakkenberg B, Sorensen F, Vesterby A, West M: The new stereological tools: disector, fractionator, nucleator and point sampled intercepts and their use in pathological research and diagnosis. APMIS 96: 379–394, 1988
15. 15 Gundersen HJ, Bendtsen TF, Korbo L, Marcussen N, Moller A. Nielsen K, Nyengaard JR, Pakkenberg B, Sorensen FB, Vesterby A, West M: Some new, simple and efficient stereological methods and their use in pathological research and diagnosis. APMIS 96: 379–394, 1988
16. 16 Howard CV, Sandau K: Measuring the surface area of a cell by the method of the spatial grid with a CSLM: a demonstration. J Microsc 165: 183–188, 1992
17. 17 Hunziker EB: Articular cartilage structure in humans and experimental animals. In: Articular Cartilage and Osteoarthritis, pp 183–199. Ed by KB Kuettner, R Schleyerbach, JG Peyron, and VC Hascall. New York, Raven Press, 1992
18. 18 Hunziker EB, Ludi A, Herrmann W: Preservation of cartilage matrix proteoglycans using cationic dyes chemically related to ruthenium hexaammine trichloride. J Histochem Cytochem 40: 909–917, 1992
19. 19 Kiviranta I, Tammi M, Jurvelin J, Säämänen A, Helminen H: Moderate running exercise augments glycosaminoglycans and thickness of articular cartilage in the knee joint of young beagle dogs. J Orthop Res 6: 188–195, 1988
20. 20 Korver GH, van de Stadt RJ, van Kampen GP, van der Korst JK: Composition of proteoglycans synthesized in different layers of cultured anatomically intact articular cartilage. Matrix 10: 394–401, 1990
21. 21 Korver THV, van de Stadt RJ, Kiljan E, van Kampen GPJ, van der Korst JK: Effects of loading on the synthesis of proteoglycans in different layers of anatomically intact articular cartilage in vitro. J Rheumatol 19: 905–912, 1992
22. 22 Lipshitz H, Etheredge R III, Glimcher M: Changes in the hexosamine content and swelling ratio of articular cartilage as functions of depth from the surface. J Bone Joint Surg [Am] 58: 1149–1153, 1976
23. 23 Majlof L, Forsgren P: Confocal microscopy: important considerations for accurate imaging. In: Cell Biological Applications of Confocal Microscopy, pp 79–95. Ed by B Matsumoto. San Diego, Academic Press, 1993
24. 24 Maroudas A, Evans H: Sulphate diffusion and incorporation by adult human articular cartilage. Biochim Biophys Acta 338: 205–279, 1974
25. 25 Maroudas A: Glycosaminoglycan turn‐over in articular cartilage. Philos Trans R Soc London [Biol] 271: 219–313, 1975
26. 26 Matsumoto B: Cell Biological Applications of Confocal Microscopy. San Diego, Academic Press, 1993
27. 27 Mayhew TM: A review of recent advances in stereology for quantifying neural structure. J Neurocytol 21: 313–328, 1992
28. 28 Muir H, Bullough P, Maroudas A: The distribution of collagen in human articular cartilage with some of its physiological implications. J Bone Joint Surg [Br] 52: 554–563, 1970
29. 29 Palmoski MJ, Brandt KD: Effects of static and cyclic compressive loading on articular cartilage plugs in vitro. Arthritis Rheum 27: 675–681, 1984
30. 30 Parkkinen JJ, Paukkonen K, Pesonen E, Lammi M, Markkanen S, Helminen HJ, Tammi M: Quantitation of autoradiographic grains in different zones of articular cartilage with image analyzer. Histochemistry 93: 241–245, 1990
31. 31 Poole CA: The structure and function of articular cartilage matrices. In: Joint Cartilage Degradation. Basic and Clinical Aspects, pp 1–35, Ed by JFJ Woessner Jr and DS Howell. New York, Marcel Dekker, 1993
32. 32 Sah RLY, Kim YJ, Doong JYH, Grodzinsky AJ, Plaas AHK, Sandy JD: Biosynthetic response of cartilage explants to dynamic compression. J Orthop Res 7: 619–636, 1989
33. 33 Sah RL‐Y, Grodzinsky A, Plaas A, Sandy J: Effects of static and dynamic compression on matrix metabolism in cartilage explants. In: Articular Cartilage and Osteoarthritis, pp 373–391. Ed by K Kuettner, R Schleyerbach, JG Peyron, and VC Hascall. New York, Raven Press, 1992
34. 34 Schneiderman R, Keret D, Maroudas A: Effects of mechanical and osmotic pressure on the rate of glycosaminoglycan synthesis in the human adult femoral head cartilage: an in vitro study. J Orthop Res 4: 393–408, 1986
35. 35 Slowman SD, Brandt KD: Composition and glycosaminoglycan metabolism of articular cartilage from habitually loaded and habitually unloaded sites. Arthritis Rheum 29: 88–94, 1986
36. 36 Sterio DC: The unbiased estimation of number and sizes of arbitrary particles using the disector. J Microsc 134: 127–136, 1984
37. 37 Stockwell RA: Inter‐relationship of articular cartilage thickness and cellularity. Ann Rheum Dis 31: 424, 1972
38. 38 Studer D, Michel M, Wagner J, Hunziker E: Vitrified articular cartilage reveals novel ultra‐structural, features respecting extracellular matrix architecture. Unpublished data
39. 39 Venn M, Maroudas A: Chemical composition and swelling of normal and osteoarthrotic femoral head cartilage. I. Chemical composition. Ann Rheum Dis 36: 121–129, 1977
40. 40 Visser T, Oud J: Volume measurements in three‐dimensional microscopy. Scanning 16: 198–200, 1993
41. 41 Weibel ER: Stereological Methods, vol 1. London, Academic Press, 1980
42. 42 Zanetti N, Solursh M: Effect of cell shape on cartilage differentiation. In: Cell Shape: Determinants, Regulation, and Regulatory Role, pp 292–327, Ed by W Stein and F Bronner. San Diego, Academic Press, 1989