NeuN is not a reliable marker of dopamine neurons in rat substantia nigra

Neuroscience Letters

Volumn 464, Issue 1, 2009, Pages 14-17

  Cannon, Jason R ^a   Greenamyre, J Timothy ^a  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

Author keywords

Dopamine neuron; NeuN; Parkinson's disease; Substantia nigra

Indexed keywords

NEURON SPECIFIC NUCLEAR PROTEIN; TYROSINE 3 MONOOXYGENASE;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL COMPARTMENTALIZATION; CELL COUNT; CELL NUCLEUS; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CYTOPLASM; DISEASE MARKER; DOPAMINERGIC NERVE CELL; MALE; MESENCEPHALON; NONHUMAN; PARKINSON DISEASE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; QUANTITATIVE ANALYSIS; RAT; SUBSTANTIA NIGRA;

ANIMALS; BIOLOGICAL MARKERS; DOPAMINE; IMMUNOHISTOCHEMISTRY; MALE; NEURONS; RATS; RATS, INBRED LEW; SUBSTANTIA NIGRA; TYROSINE 3-MONOOXYGENASE;

EID: 69249213380     PISSN: 03043940     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.neulet.2009.08.023     Document Type: Article

References (18)

1. Baquet Z.C., Bickford P.C., and Jones K.R. Brain-derived neurotrophic factor is required for the establishment of the proper number of dopaminergic neurons in the substantia nigra pars compacta. J. Neurosci. 25 (2005) 6251-6259
2. Bernheimer H., Birkmayer W., Hornykiewicz O., Jellinger K., and Seitelberger F. Brain dopamine and the syndromes of Parkinson and Huntington. Clinical, morphological and neurochemical correlations. J. Neurol. Sci. 20 (1973) 415-455
3. Betarbet R., Sherer T.B., MacKenzie G., Garcia-Osuna M., Panov A.V., and Greenamyre J.T. Chronic systemic pesticide exposure reproduces features of Parkinson's disease. Nat. Neurosci. 3 (2000) 1301-1306
4. Bowenkamp K.E., David D., Lapchak P.L., Henry M.A., Granholm A.C., Hoffer B.J., and Mahalik T.J. 6-Hydroxydopamine induces the loss of the dopaminergic phenotype in substantia nigra neurons of the rat. A possible mechanism for restoration of the nigrostriatal circuit mediated by glial cell line-derived neurotrophic factor. Exp. Brain Res. 111 (1996) 1-7
5. Cannon J.R., Tapias V., Na H.M., Honick A.S., Drolet R.E., and Greenamyre J.T. A highly reproducible rotenone model of Parkinson's disease. Neurobiol. Dis. 34 (2009) 279-290
6. Forno L.S. Neuropathology of Parkinson's disease. J. Neuropathol. Exp. Neurol. 55 (1996) 259-272
7. Lind D., Franken S., Kappler J., Jankowski J., and Schilling K. Characterization of the neuronal marker NeuN as a multiply phosphorylated antigen with discrete subcellular localization. J. Neurosci. Res. 79 (2005) 295-302
8. McCormack A.L., Thiruchelvam M., Manning-Bog A.B., Thiffault C., Langston J.W., Cory-Slechta D.A., and Di Monte D.A. Environmental risk factors and Parkinson's disease: selective degeneration of nigral dopaminergic neurons caused by the herbicide paraquat. Neurobiol. Dis. 10 (2002) 119-127
9. Mullen R.J., Buck C.R., and Smith A.M. NeuN, a neuronal specific nuclear protein in vertebrates. Development 116 (1992) 201-211
10. Novikova L., Garris B.L., Garris D.R., and Lau Y.S. Early signs of neuronal apoptosis in the substantia nigra pars compacta of the progressive neurodegenerative mouse 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine/probenecid model of Parkinson's disease. Neuroscience 140 (2006) 67-76
11. Portiansky E.L., Barbeito C.G., Gimeno E.J., Zuccolilli G.O., and Goya R.G. Loss of NeuN immunoreactivity in rat spinal cord neurons during aging. Exp. Neurol. 202 (2006) 519-521
12. Sirinathsinghji D.J., Kupsch A., Mayer E., Zivin M., Pufal D., and Oertel W.H. Cellular localization of tyrosine hydroxylase mRNA and cholecystokinin mRNA-containing cells in the ventral mesencephalon of the common marmoset: effects of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine. Brain Res. Mol. Brain Res. 12 (1992) 267-274
13. Unal-Cevik I., Kilinc M., Gursoy-Ozdemir Y., Gurer G., and Dalkara T. Loss of NeuN immunoreactivity after cerebral ischemia does not indicate neuronal cell loss: a cautionary note. Brain Res. 1015 (2004) 169-174
14. Ungerstedt U. 6-Hydroxy-dopamine induced degeneration of central monoamine neurons. Eur. J. Pharmacol. 5 (1968) 107-110
15. Varastet M., Riche D., Maziere M., and Hantraye P. Chronic MPTP treatment reproduces in baboons the differential vulnerability of mesencephalic dopaminergic neurons observed in Parkinson's disease. Neuroscience 63 (1994) 47-56
16. West M.J., Slomianka L., and Gundersen H.J. Unbiased stereological estimation of the total number of neurons in the subdivisions of the rat hippocampus using the optical fractionator. Anat. Rec. 231 (1991) 482-497
17. Wolf H.K., Buslei R., Schmidt-Kastner R., Schmidt-Kastner P.K., Pietsch T., Wiestler O.D., and Blumcke I. NeuN: a useful neuronal marker for diagnostic histopathology. J. Histochem. Cytochem. 44 (1996) 1167-1171
18. Zhu C., Vourc'h P., Fernagut P.O., Fleming S.M., Lacan S., Dicarlo C.D., Seaman R.L., and Chesselet M.F. Variable effects of chronic subcutaneous administration of rotenone on striatal histology. J. Comp. Neurol. 478 (2004) 418-426