Novel monoclonal antibodies detect Smad-interacting protein 1 (SIP1) in the cytoplasm of human cells from multiple tumor tissue arrays

Experimental and Molecular Pathology

Volumn 89, Issue 2, 2010, Pages 182-189

  Oztas, Emin ^a,b   Avci, M Ender ^a   Ozcan, Ayhan ^b   Sayan, A Emre ^c,d   Tulchinsky, Eugene ^c   Yagci, Tamer ^a  

^a BILKENT UNIVERSITY   (Turkey)

^b GULHANE SCHOOL OF MEDICINE   (Turkey)

^c UNIVERSITY OF LEICESTER   (United Kingdom)

^d UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

Author keywords

Immunohistochemistry; Monoclonal antibodies; Multiple tissue arrays; SIP1 ZEB2

Indexed keywords

BINDING PROTEIN; MONOCLONAL ANTIBODY; PROTEIN ZEB1; SMAD INTERACTING PROTEIN 1; UNCLASSIFIED DRUG; UVOMORULIN; CADHERIN; HOMEODOMAIN PROTEIN; REPRESSOR PROTEIN; ZEB2 PROTEIN, HUMAN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANTIBODY SPECIFICITY; ARTICLE; BREAST TUMOR; CARCINOGENESIS; CELL NUCLEUS; CELLULAR DISTRIBUTION; COLON MUCOSA; COLON TUMOR; CONTROLLED STUDY; CYTOPLASM; ESOPHAGUS MUCOSA; ESOPHAGUS TUMOR; HUMAN; HUMAN CELL; HUMAN TISSUE; KIDNEY DISTAL TUBULE; KIDNEY TUMOR; LIVER CELL; LIVER TUMOR; LUNG TUMOR; MOUSE; NONHUMAN; OSTEOSARCOMA CELL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; RECTUM MUCOSA; RECTUM TUMOR; STOMACH TUMOR; TISSUE MICROARRAY; UTERUS TUMOR; ANIMAL; BAGG ALBINO MOUSE; CELL LINE; COMPARATIVE STUDY; EPITHELIUM; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE; GENETICS; IMMUNOHISTOCHEMISTRY; IMMUNOLOGY; METABOLISM; NEOPLASM; VALIDATION STUDY;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; CADHERINS; CELL LINE; CYTOPLASM; EPITHELIUM; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE; HOMEODOMAIN PROTEINS; HUMANS; IMMUNOHISTOCHEMISTRY; MICE; MICE, INBRED BALB C; NEOPLASMS; REPRESSOR PROTEINS;

EID: 77956432540     PISSN: 00144800     EISSN: 10960945     Source Type: Journal    
DOI: 10.1016/j.yexmp.2010.05.010     Document Type: Article

References (33)

1. Avci M.E., et al. Quantification of SLIT-ROBO transcripts in hepatocellular carcinoma reveals two groups of genes with coordinate expression. BMC Cancer 2008, 8:392.
2. Bassez G., et al. Pleiotropic and diverse expression of ZFHX1B gene transcripts during mouse and human development supports the various clinical manifestations of the "Mowat-Wilson" syndrome. Neurobiol. Dis. 2004, 15:240-250.
3. Bindels S., et al. Regulation of vimentin by SIP1 in human epithelial breast tumor cells. Oncogene 2006, 25:4975-4985.
4. Celikkaya H., et al. Immunization with UV-induced apoptotic cells generates monoclonal antibodies against proteins differentially expressed in hepatocellular carcinoma cell lines. Hybridoma (Larchmt) 2007, 26:55-61.
5. Chen C.L., et al. Systemic evaluation of total Stat3 and Stat3 tyrosine phosphorylation in normal human tissues. Exp. Mol. Pathol. 2006, 80:295-305.
6. Comijn J., et al. The two-handed E box binding zinc finger protein SIP1 downregulates E-cadherin and induces invasion. Mol. Cell 2001, 7:1267-1278.
7. Elloul S., et al. Snail, Slug, and Smad-interacting protein 1 as novel parameters of disease aggressiveness in metastatic ovarian and breast carcinoma. Cancer 2005, 103:1631-1643.
8. Gregory P.A., et al. The miR-200 family and miR-205 regulate epithelial to mesenchymal transition by targeting ZEB1 and SIP1. Nat. Cell Biol. 2008, 10:593-601.
9. Imamichi Y., et al. Collagen type I-induced Smad-interacting protein 1 expression downregulates E-cadherin in pancreatic cancer. Oncogene 2007, 26:2381-2385.
10. Isohata N., et al. Hedgehog and epithelial-mesenchymal transition signaling in normal and malignant epithelial cells of the esophagus. Int. J. Cancer 2009, 125:1212-1221.
11. Krishnamachary B., et al. Hypoxia-inducible factor-1-dependent repression of E-cadherin in von Hippel-Lindau tumor suppressor-null renal cell carcinoma mediated by TCF3, ZFHX1A, and ZFHX1B. Cancer Res. 2006, 66:2725-2731.
12. Leibovitz A., et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 1976, 36:4562-4569.
13. Lin L.I., et al. Curcumin inhibits SK-Hep-1 hepatocellular carcinoma cell invasion in vitro and suppresses matrix metalloproteinase-9 secretion. Oncology 1998, 55:349-353.
14. Lin S.Y., Elledge S.J. Multiple tumor suppressor pathways negatively regulate telomerase. Cell 2003, 113:881-889.
15. Long J., et al. Pc2-mediated sumoylation of Smad-interacting protein 1 attenuates transcriptional repression of E-cadherin. J. Biol. Chem. 2005, 280:35477-35489.
16. Maeda G., et al. Expression of SIP1 in oral squamous cell carcinomas: implications for E-cadherin expression and tumor progression. Int. J. Oncol. 2005, 27:1535-1541.
17. Massague J. TGFbeta in cancer. Cell 2008, 134:215-230.
18. Mejlvang J., et al. Direct repression of cyclin D1 by SIP1 attenuates cell cycle progression in cells undergoing an epithelial mesenchymal transition. Mol. Biol. Cell 2007, 18:4615-4624.
19. Mikkers H., et al. High-throughput retroviral tagging to identify components of specific signaling pathways in cancer. Nat. Genet. 2002, 32:153-159.
20. Miyoshi A., et al. Snail and SIP1 increase cancer invasion by upregulating MMP family in hepatocellular carcinoma cells. Br. J. Cancer 2004, 90:1265-1273.
21. Ozturk N., et al. Reprogramming of replicative senescence in hepatocellular carcinoma-derived cells. Proc. Natl. Acad. Sci. U. S. A. 2006, 103:2178-2183.
22. Park S.M., et al. The miR-200 family determines the epithelial phenotype of cancer cells by targeting the E-cadherin repressors ZEB1 and ZEB2. Genes Dev. 2008, 22:894-907.
23. Postigo A.A. Opposing functions of ZEB proteins in the regulation of the TGFbeta/BMP signaling pathway. EMBO J. 2003, 22:2443-2452.
24. Postigo A.A., et al. Regulation of Smad signaling through a differential recruitment of coactivators and corepressors by ZEB proteins. EMBO J. 2003, 22:2453-2462.
25. Remacle J.E., et al. New mode of DNA binding of multi-zinc finger transcription factors: deltaEF1 family members bind with two hands to two target sites. EMBO J. 1999, 18:5073-5084.
26. Rosivatz E., et al. Differential expression of the epithelial-mesenchymal transition regulators snail, SIP1, and twist in gastric cancer. Am. J. Pathol. 2002, 161:1881-1891.
27. Sayan A.E., et al. SIP1 protein protects cells from DNA damage-induced apoptosis and has independent prognostic value in bladder cancer. Proc. Natl. Acad. Sci. U. S. A. 2009.
28. Sivertsen S., et al. Expression of Snail, Slug and Sip1 in malignant mesothelioma effusions is associated with matrix metalloproteinase, but not with cadherin expression. Lung Cancer 2006, 54:309-317.
29. van Grunsven L.A., et al. Interaction between Smad-interacting protein-1 and the corepressor C-terminal binding protein is dispensable for transcriptional repression of E-cadherin. J. Biol. Chem. 2003, 278:26135-26145.
30. Vandewalle C., et al. SIP1/ZEB2 induces EMT by repressing genes of different epithelial cell-cell junctions. Nucleic Acids Res. 2005, 33:6566-6578.
31. Vandewalle C., et al. The role of the ZEB family of transcription factors in development and disease. Cell. Mol. Life Sci. 2009, 66:773-787.
32. Verschueren K., et al. SIP1, a novel zinc finger/homeodomain repressor, interacts with Smad proteins and binds to 5'-CACCT sequences in candidate target genes. J. Biol. Chem. 1999, 274:20489-20498.
33. Yoshida J., et al. Changes in the expression of E-cadherin repressors, Snail, Slug, SIP1, and Twist, in the development and progression of ovarian carcinoma: the important role of Snail in ovarian tumorigenesis and progression. Med. Mol. Morphol. 2009, 42:82-91.