In vitro culture of Taxus sp.: Strategies to increase cell growth and taxoid production

Phytochemistry Reviews

Volumn 9, Issue 2, 2010, Pages 343-356

  Sabater Jara, A B ^a   Tudela, L R ^b   Lopez Perez A J ^b  

^a UNIVERSITY OF MURCIA   (Spain)

^b RandD Department of Plant Biotechnology   (Spain)

Author keywords

Biotechnology; Paclitaxel accumulation; Plant cell culture; Secondary metabolite production

Indexed keywords

10 DEACETYLBACCATIN III; 10 DEACETYLPACLITAXEL; ANTIOXIDANT; BACCATIN III; CEPHALOMANNINE; NATURAL PRODUCT; ORGANIC COMPOUND; PACLITAXEL; PLANT EXTRACT; PLANT MEDICINAL PRODUCT; TAXADIENE; TAXANE DERIVATIVE; TAXOID; TAXUS EXTRACT; UNCLASSIFIED DRUG; VITAMIN;

ANTINEOPLASTIC ACTIVITY; BIOENGINEERING; CELL ACTIVITY; CELL GROWTH; CELL STRESS; CELL VIABILITY; CULTURE MEDIUM; CULTURE TECHNIQUE; DRUG ISOLATION; DRUG PURIFICATION; DRUG SYNTHESIS; IN VIVO CULTURE; NONHUMAN; PLANT BIOTECHNOLOGY; PLANT CELL CULTURE; PLANT STEM; PRIORITY JOURNAL; REVIEW; TAXUS;

TAXUS; TAXUS BREVIFOLIA;

EID: 77956387653     PISSN: 15687767     EISSN: 1572980X     Source Type: Journal    
DOI: 10.1007/s11101-010-9167-z     Document Type: Review

References (79)

1. Š Baebler M Hren M Camloh M Ravnijar B Bohanec I Placer R Ucman J Žel 2005 Establishment of cell suspension cultures of yew (Taxus x media rehd.) and assessment of their genomic stability In Vitro Cell Dev Biol Plant 41 338 343 10.1079/IVP2005642 1:CAS:528:DC%2BD2MXos1alur8%3D
2. J Bai M Kitabatake K Toyoizumi L Fu S Zhang J Dai J Sakai K Hirose T Yamori A Tomida T Tsuruo M Ando 2004 Production of biologically active taxoids by a callus culture of Taxus cuspidata J Nat Prod 67 58 63 10.1021/np0301083 14738387 1:CAS:528:DC%2BD3sXps1Snu7c%3D
3. S Bentebibel E Moyano J Palazón RM Cusidó M Bonfill R Eibl MT Piñol 2005 Effect of immobilization by entrapment in alginate and scale-up in paclitaxel and baccatin III production in cell suspension cultures of Taxus baccata Biotechnol Bioeng 89 647 655 10.1002/bit.20321 15696535 1:CAS:528:DC%2BD2MXitFyhtLw%3D
4. O Besumbes S Sauret-Güeto MA Phillips S Imperial M Rodríguez-Concepción A Boronat 2004 Metabolic engineering of isoprenoid biosynthesis in Arabidopsis for the production of taxadiene, the first committed precursor of Taxol Biotechnol Bioeng 88 168 175 10.1002/bit.20237 15449291 1:CAS:528:DC%2BD2cXot1Kisr4%3D
5. NL Biddington 1992 The influence of ethylene in plant tissue culture Plant Growth Regul 11 173 187 10.1007/BF00024072 1:CAS:528:DyaK38XitFWisrk%3D
6. M Bonfill J Palazón RM Cusidó S Joly C Morales MT Piñol 2003 Influence of elicitors on taxane production and 3-hydroxy-3-methylglutaril coenzyme A reductase activity in Taxus media cells Plant Physiol Biochem 41 91 96 10.1016/S0981-9428(02)00013-X 1:CAS:528:DC%2BD3sXhs1Cku7s%3D
7. M Bonfill S Bentebibel E Moyano J Palazón RM Cusidó R Eibl MT Piñol 2007 Paclitaxel and baccatin III production induced by methyl jasmonate in free and immobilized cells of Taxus baccata Biol Plantarum 51 647 652 10.1007/s10535-007-0137-2 1:CAS:528:DC%2BD1cXislGhs7c%3D
8. JA Chemler MAG Koffas 2008 Metabolic engineering for plant natural product biosynthesis in microbes Curr Opin Biotechnol 19 597 605 10.1016/j.copbio.2008.10.011 18992815 1:CAS:528:DC%2BD1cXhsVKrtLjL
9. YQ Chen F Yi M Cai JX Luo 2003 Effects of amino acids, nitrate and ammonium on the growth and taxol production in cell cultures of Taxus yunnanensis Plant Growth Regul 41 265 268 10.1023/B:GROW.0000007502.72108.e3 1:CAS:528:DC%2BD3sXps1amsr4%3D
10. JS Cheng QM Xu JC Wu YJ Yuan 2008 ERK-like MAPK signalling and cytochrome c response to oleic acid in two-liquid-phase suspension cultures of Taxus cuspidata Biochem Eng J 41 274 279 10.1016/j.bej.2008.05.010 1:CAS:528:DC%2BD1cXhtVSgtL7M
11. HK Choi SI Kim JS Son SS Hong HS Lee HJ Lee 2000 Enhancement of paclitaxel production by temperature shift in suspension culture of Taxus chinensis Enzyme Microb Technol 27 593 598 10.1016/S0141-0229(00)00255-6 11024522 1:CAS:528:DC%2BD3cXnt1SjsLo%3D
12. HK Choi SI Kim JS Son SS Hong HS Lee IS Chung HJ Lee 2000 Intermittent maltose feeding enhances paclitacxel production in suspension culture of Taxus chinensis cells Biotechnol Lett 22 1793 1796 10.1023/A:1005658405449 1:CAS:528:DC%2BD3cXovVyksbs%3D
13. RM Cusidó J Plazón A Navia-Osorio A Mallol M Bonfill C Morales MT Piñol 1999 Production of taxol and baccatin III by a selected Taxus baccata callus line and its derived cell suspension culture Plant Sci 146 101 107 10.1016/S0168-9452(99)00093-X
14. RM Cusidó J Palazón M Bonfill A Navia-Osorio C Morales MT Piñol 2002 Improved paclitaxel and baccatin III production in suspension cultures of Taxus media Biotechnol Prog 18 418 423 10.1021/bp0101583 12052053 1:CAS:528:DC%2BD38Xjt1KrsL8%3D
15. MM Datta A Majunder S Jha 2006 Organogenesis and plant regeneration in Taxus wallichiana (Zucc.) Plant Cell Rep 25 11 18 10.1007/s00299-005-0027-z 16240121 1:CAS:528:DC%2BD28Xnt1Cjug%3D%3D
16. JF Diaz I Barasoain AA Souto F Amat-Guerri JM Andreu 2005 Macromolecular accessibility of fluorescent taxoids bound at a paclitaxel binding site in the microtubule surface J Biol Chem 280 3928 3937 10.1074/jbc.M407816200 15550392 1:CAS:528:DC%2BD2MXovVGksg%3D%3D
17. HD Dong JJ Zhong 2001 Significant improvement of taxane production in suspension cultures of Taxus chinensis by combining elicitation with sucrose feed Biochem Eng J 8 145 150 10.1016/S1369-703X(01)00096-1 1:CAS:528: DC%2BD3MXltlyqsrY%3D
18. U Eilert WGW Kurz F Constabel 1985 Stimulation of sanguinarine accumulation in Papaver somniferum cell cultures by fungal elicitors J Plant Physiol 119 65 76 1:CAS:528:DyaL2MXktVeku78%3D
19. B Engels P Dahmb S Jennewein 2008 Metabolic engineering of taxadiene biosynthesis in yeast as a first step towards Taxol (Paclitaxel) production Metab Eng 10 2008 201 206 10.1016/j.ymben.2008.03.001 18485776 1:CAS:528:DC%2BD1cXnt1ekur8%3D
20. O Expósito M Bonfill M Onrubia A Jané E Moyano RM Cusidó J Palazón MT Piñol 2009 Effect of taxol feeding on paclitaxel and related taxane production in Taxus baccata suspension cultures New Biotech 25 252 259 10.1016/j.nbt.2008.11.001 1:CAS:528:DC%2BD1MXltlersLY%3D
21. AG Fett-Neto F DiCosmo WF Reynolds K Sakata 1992 Cell culture of Taxus as a source of the antineoplasic drug taxol and related taxanes Bio/Technology 10 1572 1575 10.1038/nbt1292-1572 1369501 1:CAS:528:DyaK3sXhtVSnt74%3D
22. AG Fett-Neto SJ Melanson K Sakata F DiCosmo 1993 Improved growth and taxol yield in developing calli of Taxus cuspidata by medium composition modification Bio/Technolog 11 731 734 10.1038/nbt0693-731 1:CAS:528: DyaK3sXlsFyhu7g%3D
23. D Frense 2007 Taxanes: perspectives for biotechnological production Appl Microbiol Biotechnol 73 1233 1240 10.1007/s00253-006-0711-0 17124581 1:CAS:528:DC%2BD2sXjsFKktw%3D%3D
24. Fujita Y, Tabata M (1987) Secondary metabolites from plant cells-pharmaceuticals applications and progress in commercial production. In: Green CE, Somers DA, Hackett WP, Biesboer DD (eds) Plant tissue and cell culture. Alan R Liss, New York, pp 169-185
25. M Furmanova H Olȩdzka K Sykłowska-Baranek J Jósefowicz S Gieraka 2000 Increased taxane accumulation in callus cultures of Taxus cuspidata and Taxus x media by some elicitors and precursors Biotechnol Lett 22 1449 1452 10.1023/A:1005611114628
26. OL Gamborg RA Miller K Ojima 1968 Nutrient requirement of suspension cultures for soybean root cells Exp Cell Res 50 151 158 10.1016/0014-4827(68) 90403-5 5650857 1:CAS:528:DyaF1cXktVyqtLw%3D
27. DM Gibson REB Ketchum NC Vance AA Christen 1993 Initiation and growth of cell lines of Taxus brevifolia (Pacific yew) Plant Cell Rep 12 479 482 10.1007/BF00236091 1:CAS:528:DyaK3sXlvF2lur4%3D
28. M Hezari REB Ketchum DM Gibson R Croteau 1997 Taxol production and taxadiene synthase activity in Taxus canadiensis cell suspension culture Arch Biochem Biophys 337 185 190 10.1006/abbi.1996.9772 9016812 1:CAS:528: DyaK2sXlsVGkug%3D%3D
29. QL Huang CA Roessner R Croteau AI Scott 2001 Engineering Escherichia coli for the synthesis of taxadiene, a key intermediate in the biosynthesis of taxol Bioorg Med Chem 9 2237 2242 10.1016/S0968-0896(01)00072-4 11553461 1:CAS:528:DC%2BD3MXmslartLw%3D
30. S Jennewein RM Long RM Williams R Croteau 2004 Cytochrome P450 taxadiene 5a-hydroxylase, a mechanistically unusual monooxygenase catalyzing the first oxygenation step of Taxol biosynthesis Chem Biol 11 379 387 10.1016/j.chembiol. 2004.02.022 15123267 1:CAS:528:DC%2BD2cXis1WntLc%3D
31. REB Ketchum DM Gibson RB Croteau ML Shuler 1999 The kinetics of taxoid accumulation in cell suspension cultures of Taxus following elicitation with methyl jasmonate Biotechnol Bioeng 62 97 105 10.1002/(SICI)1097-0290(19990105) 62:1<97:AID-BIT11>3.0.CO;2-C 10099517 1:CAS:528:DyaK1cXnslajtbk%3D
32. REB Ketchum L Wherland RB Croteau 2007 Stable transformation and long-term maintenance of transgenic Taxus cell suspension cultures Plant Cell Rep 26 1025 1033 10.1007/s00299-007-0323-x 17333018 1:CAS:528: DC%2BD2sXnsFaqtbg%3D
33. DI Kim H Pedersen CK Chin 1991 Cultivation of Thalictrum rugosum cell suspension in an improved airlift bioreactor: stimulatory effect of carbon dioxide and ethylene on alkaloid production Biotechnol Bioeng 38 331 339 10.1002/bit.260380403 18600769 1:CAS:528:DyaK3MXltFaktbY%3D
34. SI Kim HK Choi JH Kim HS Lee SS Hong 2001 Effect of osmotic pressure on paclitaxel production in suspension cell cultures of Taxus chinensis Enzyme Microb Technol 28 202 209 10.1016/S0141-0229(00)00292-1 11166813 1:CAS:528:DC%2BD3MXptlSisw%3D%3D
35. BJ Kim DM Gibson ML Shuler 2005 Relationship of viability and apoptosis to taxol production in Taxus sp. suspension cultures elicited with methyl jasmonate Biotechnol Prog 21 700 707 10.1021/bp050016z 15932245 1:CAS:528:DC%2BD2MXisleqs78%3D
36. VI Knight H Wang JE Lincoln EC Lulai DG Gilchrist RM Bostock 2001 Hydroperoxides of fatty acids induce programmed cell death in tomato protoplasts Physiol Mol Plant Pathol 59 277 286 10.1006/pmpp.2001.0366 1:CAS:528: DC%2BD38Xht1Gnsrk%3D
37. AE Koepp M Hezari B Zajicek B Stofer Vogel RE Lafever NG Lewis R Croteau 1995 Cyclization of geranylgeranyl diphosphate to taxa-4(5), 11(12)-diene is the committed step of taxol biosynthesis in Pacific yew J Biol Chem 270 8686 8690 10.1074/jbc.270.15.8686 7721772 1:CAS:528:DyaK2MXltVOgu7s%3D
38. ME Kolewe V Gaurav SC Roberts 2008 Pharmaceuticals active natural products synthesis and supply via plant cell culture technology Mol Pharm 5 243 256 10.1021/mp7001494 18393488 1:CAS:528:DC%2BD1cXkt1CktLg%3D
39. K Kovacs L Zhang RST Linforth B Whittaker CJ Hayes RG Fray 2007 Redirection of carotenoid metabolism for the efficient production of taxadiene [taxa-4(5), 11(12)-diene] in transgenic tomato fruit Transgenic Res 16 121 126 10.1007/s11248-006-9039-x 17106766 1:CAS:528:DC%2BD2sXitFWhtQ%3D%3D
40. WZ Lan LJ Yu P Cheng MY Li 2002 Improvement of cephalomanine production in Taxus chinensis cells by a combination of sucrose and methyl jasmonate Biotechnol Lett 24 1253 1255 10.1023/A:1016269627346 1:CAS:528: DC%2BD38XltFanu74%3D
41. A Lansing M Haertel M Gordon HG Floss 1991 Biosynthetic studies on taxol Planta Med 57 83 10.1055/s-2006-960355
42. G Laskaris M Bounkhay G Theodoridis R van der Heijden R Verpoorte M Jaziri 1999 Induction of geranylgeranyl diphosphate synthase activity and taxane accumulation in Taxus baccata cell cultures after elicitation by methyl jasmonate Plant Sci 147 1 8 10.1016/S0168-9452(99)00078-3 1:CAS:528: DyaK1MXls1yqt7o%3D
43. F Lavelle C Combeau A Commercon 1995 Taxoids: structural and experimental properties Bull Cancer 82 249 264 10846536 1:STN:280:DC%2BD3czgsFyqsw%3D%3D
44. J Luo GY He 2004 Optimization of elicitors and precursors for paclitaxel production in cell suspension culture of Taxus chinensis in the presence of nutrient feeding Process Biochem 39 1073 1079 10.1016/S0032-9592(03)00219-X 1:CAS:528:DC%2BD2cXjtl2lu74%3D
45. J Luo L Liu CD Wu 2001 Enhancement of paclitaxel production by abscisic acid in cell suspension cultures of Taxus chinensis Biotechnol Lett 23 1345 1348 10.1023/A:1010597802741 1:CAS:528:DC%2BD3MXms12hsL4%3D
46. J Luo XG Mei L Liu DW Hu 2002 Improved paclitaxel production by fed-batch suspension cultures of Taxus chinensis in bioreactors Biotechnol Lett 24 561 565 10.1023/A:1014596924446 1:CAS:528:DC%2BD38Xjt1Gmurc%3D
47. ZY Ma YJ Yuan JC Wu AP Zeng 2002 Cell death in suspension cultures of Taxus chinensis var. mairei Biotechnol Lett 24 573 577 10.1023/A:1014820810258 1:CAS:528:DC%2BD38Xjt1Gmu78%3D
48. N Mirjalili J Linden 1996 Methyl jamonate induced production of taxol in suspension cultures of Taxus cuspidata: ethylene interaction and induction models Biotechnol Prog 12 110 118 10.1021/bp9500831 8845101 1:CAS:528: DyaK28XjsVOhsw%3D%3D
49. T Murashige F Skoog 1962 A revised medium for rapid growth and bioassays with tobacco tissue cultures Physiol Plant 15 473 497 10.1111/j.1399-3054.1962. tb08052.x 1:CAS:528:DyaF3sXksFKm
50. Namdeo AG (2007) Plant cell elicitation for production of secondary metabolites: a review. Phcog rev 1:69-79. (www.phcog.rev.com)
51. A Navia-Osorio H Garden RM Cusidó J Palazón AW Alfermann MT Piñol 2002 Taxol® and baccatin III production in suspension cultures of Taxus baccata and Taxus wallichiana in an airlift bioreactor J Plant Physiol 159 97 102 10.1078/0176-1617-00576 1:CAS:528:DC%2BD38XhslOktrs%3D
52. E Nims CP Dubois SC Roberts EL Walker 2006 Expression profiling of genes involved in paclitaxel biosynthesis for targeted metabolic engineering Metab Eng 8 385 394 10.1016/j.ymben.2006.04.001 16793302 1:CAS:528:DC%2BD28XoslOgsbc%3D
53. ZW Pan HQ Wang JJ Zhong 2000 Scale-up study on suspension cultures of Taxus chinensis cells for production of taxane diterpene Enzyme Microb Technol 27 714 723 10.1016/S0141-0229(00)00276-3 11064055 1:CAS:528:DC%2BD3cXotlGjt70%3D
54. S Pant SS Samant 2008 Population ecology of the endangered Himalayan yew in Khokhan wildlife sanctuary of north western Himalaya for conservation management J Mountain Sci 3 257 264 10.1007/s11629-008-0078-z
55. L Pestchanker S Roberts M Shuler 1996 Kinetics of Taxol production and nutrient use in suspension cultures of Taxus cuspidata in shake flasks and Wilson-Type bioreactor Enzyme Microb Technol 19 256 260 10.1016/0141-0229(95) 00243-X 8987485 1:CAS:528:DyaK28XltVOju78%3D
56. M Phisalaphong JC Linden 1999 Kinetic studies of paclitaxel production by Taxus canadiensis cultures in batch and semicontinuous with total cell recycle Biotechnol Prog 15 1072 1077 10.1021/bp990098p 10585192 1:CAS:528: DyaK1MXlt12rtLc%3D
57. ZG Qian ZJ Zhao WH Tian Y Xu JJ Zhong X Qian 2003 Novel synthetic jasmonates as highly efficient elicitors for taxoids production by suspension cultures of Taxus chinensis Biotechnol Bioeng 86 595 599 10.1002/bit.20068 1:CAS:528:DC%2BD2cXkt1Cgsrk%3D
58. JJ Qiao YJ Yuan H Zhao JC Wu AP Zeng 2003 Apoptotic cell death in suspension cultures of Taxus cuspidata co-treated with salicylic acid and hydrogen peroxide Biotechnol Lett 25 387 390 10.1023/A:1022494213446 12882558 1:CAS:528:DC%2BD3sXht1Wktrc%3D
59. I Raskin DM Ribnicky S Kormarnytsky N Ilic A Poulev N Borisjuk A Brinker DA Moreno Ch Ripoll N Yakoby JM O'Neal T Cornwell I Pastor B Fridlender 2002 Plants and human health in the twenty-first century Trends Biotechnol 20 522 531 10.1016/S0167-7799(02)02080-2 12443874 1:CAS:528:DC%2BD38XoslCgtLc%3D
60. SC Roberts 2007 Production and engineering of terpenoids in plant cell culture Nat Chem Biol 3 387 395 10.1038/nchembio.2007.8 17576426 1:CAS:528:DC%2BD2sXms1Srsb0%3D
61. RU Schenk AC Hildebrandt 1972 Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures Can J Bot 50 199 204 10.1139/b72-026 1:CAS:528:DyaE38XmvFOgsQ%3D%3D
62. JE Schlatmann E Fonck HJG ten Hoopen JJ Heijnen 1994 The negligible role of carbon dioxide and ethylene in ajmalicine production by Catharanthus roseus cell suspensions Plant Cell Rep 14 157 160 10.1007/BF00233781 1:CAS:528:DyaK2MXjvFGnsL8%3D
63. D Schrijvers JB Vermorken 2000 Role of taxoids in head and neck cancer Oncologist 5 199 208 10.1634/theoncologist.5-3-199 10884498 1:CAS:528: DC%2BD3cXltFSju78%3D
64. M Solomon B Belenghi M Delledonne E Menachem A Levine 1999 The involvement of cysteine protease and protease inhibitor genes in the regulation of programmed cell death in plants Plant Cell 11 431 443 10.1105/tpc.11.3.431 10072402 1:CAS:528:DyaK1MXitlehs7c%3D
65. R Verpoorte A Contin J Memelink 2002 Biotechnology for the production of plant secondary metabolites Phytochem Rev 1 13 25 10.1023/A:1015871916833 1:CAS:528:DC%2BD38XmsV2isLs%3D
66. HQ Wang JT Yu JJ Zhong 1999 Significant improvement of taxane production in suspension cultures of Taxus chinensis by sucrose feeding strategy Process Biochem 35 479 483 10.1016/S0032-9592(99)00094-1
67. Ch Wang J Wu X Mei 2001 Enhanced taxol production and release in Taxus chinensis cell suspension cultures with selected organic solvents and sucrose feeding Biotechnol Prog 17 89 94 10.1021/bp0001359 11170485 1:CAS:528: DC%2BD3cXosVant78%3D
68. Ch Wang J Wu X Mei 2001 Enhancement of taxol production and excretion in Taxus chinensis cell cultures by fungal elicitation and medium renewal Appl Microbiol Biotechnol 55 404 410 10.1007/s002530000567 11398918 1:CAS:528:DC%2BD3MXktlOhurY%3D
69. ERM Wickremesinhe RN Arteca 1994 Taxus cell suspension cultures: optimizing growth and production of Taxol J Plant Physiol 144 183 188 1:CAS:528:DyaK2cXlsFGrs70%3D
70. ZL Wu YJ Yuan ZH Ma ZD Hu 2000 Kinetics of two-liquid-phase Taxus cuspidata cell culture for production of Taxol Biochem Eng J 5 137 142 10.1016/S1369-703X(00)00052-8 10817819 1:CAS:528:DC%2BD3cXkt1eqt78%3D
71. J Wu Wang Ch X Mei 2001 Stimulation of taxol production and excretion in Taxus cell cultures by rare earth chemical lanthanum J Biotech 85 67 73 10.1016/S0168-1656(00)00383-7 1:CAS:528:DC%2BD3MXntl2mtA%3D%3D
72. QM Xu JS Cheng ZQ Ge YJ Yuan 2004 Effects of organic solvents on membrane of Taxus cuspidata cells in two-liquid-phase cultures Plant Cell Tiss Org Cult 79 63 69 10.1007/s11240-004-4709-y 1:CAS:528:DC%2BD2cXhtVaisLzP
73. QM Xu JS Cheng ZQ Ge YJ Yuan 2005 Abnormal mitosis versus apoptosis of Taxus cuspidata induced by oleic acid in two-liquid-phase suspension cultures Enzyme Microb Tech 37 76 81 10.1016/j.enzmictec.2005.01.035 1:CAS:528: DC%2BD2MXjvVKqs7k%3D
74. LJ Yu WZ Lan WM Qin HB Xu 2001 Effects of salicylic acid on fungal elicitor-induced membrane-lipid peroxidation and taxol production in cell suspension cultures of Taxus chinensis Prog Biochem 37 477 482 10.1016/S0032-9592(01)00243-6
75. YJ Yuan C Li ZD Hu JC Wu AP Zeng 2002 Fungal elicitor-induced cell apoptosis in suspension cultures of Taxus chinensis var. mairei for taxol production Process Biochem 38 193 198 10.1016/S0032-9592(02)00071-7 1:CAS:528:DC%2BD38XltlGmtbk%3D
76. LO Zamir ME Nedea S Belair F Sauriol O Mamer E Jacqmain FI Jean FX Garneau 1992 Biosynthetic building blocks of Taxus canadiensis taxanes Tetrahedron Lett 33 5235 5236 10.1016/S0040-4039(00)79141-6 1:CAS:528: DyaK38XmtlSiur0%3D
77. CH Zang HB Xu 2001 Improved paclitaxel production by in situ extraction and elicitation in cell suspension cultures of Taxus chinensis Biotechnol Lett 23 189 193 10.1023/A:1005655219649
78. JJ Zhong 2002 Plant cell culture for production of paclitaxel and other taxanes J Biosci Bioeng 94 591 599 16233355 1:CAS:528:DC%2BD3sXhsVOnsb8%3D
79. JJ Zhong M Yoshida K Fujiyama T Seki T Yoshida 1993 Enhancement of anthocyanin production by Perilla frutescens cells in a stirred bioreactor with internal light irradiation J Ferment Bioeng 15 299 303 10.1016/0922-338X(93) 90155-2