Ultra performance liquid chromatography analysis of flunitrazepam and 7-aminoflunitrazepam in human plasma

Journal of Liquid Chromatography and Related Technologies

Volumn 33, Issue 14, 2010, Pages 1381-1392

  Kiss, Bela ^a   Bogdan, Catalina ^a   Pop, Anca ^a   Loghin, Felicia ^a  

^a IULIU HATIEGANU UNIVERSITY OF MEDICINE AND PHARMACY   (Romania)

Author keywords

7 aminoflunitrazepam; flunitrazepam; plasma; ultra performance liquid chromatography; validation

Indexed keywords

7-AMINOFLUNITRAZEPAM; ANALYTES; CHROMATOGRAPHIC COLUMN; CHROMATOGRAPHIC SEPARATIONS; ETHYL ACETATES; FLUNITRAZEPAM; GRADIENT ELUTION; HIGH-THROUGHPUT; HUMAN PLASMA SAMPLES; HUMAN PLASMAS; MOBILE PHASE; RUNTIMES; SIMPLE LIQUIDS; SINGLE-DOSE; ULTRAPERFORMANCE LIQUID CHROMATOGRAPHY; UV DETECTION; VALIDATION;

BINARY MIXTURES; CHROMATOGRAPHIC ANALYSIS; ESTERS; LIQUIDS; PLASMA (HUMAN); PLASMAS; POTASSIUM; SOLVENT EXTRACTION;

LIQUID CHROMATOGRAPHY;

7 AMINOFLUNITRAZEPAM; ACETIC ACID ETHYL ESTER; ACETONITRILE; CHLORDIAZEPOXIDE; FLUNITRAZEPAM; PHOSPHATE; POTASSIUM MONOHYDROGEN PHOSPHATE; UNCLASSIFIED DRUG;

ADULT; ARTICLE; BLOOD SAMPLING; DRUG BLOOD LEVEL; ELUTION; FEMALE; FLOW RATE; HUMAN; HUMAN EXPERIMENT; LIQUID LIQUID EXTRACTION; PH; QUANTITATIVE ANALYSIS; SINGLE DRUG DOSE; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY; ULTRAVIOLET RADIATION;

EID: 77956291908     PISSN: 10826076     EISSN: 1520572X     Source Type: Journal    
DOI: 10.1080/10826076.2010.489441     Document Type: Article

References (26)

1. Druid, H.; Holmgren, P.; Ahlner, J. Flunitrazepam: An Evaluation of Use, Abuse and Toxicity. Forensic Sci. Int. 2001, 122, 136-141.
2. Ohshima, T. A Case of Drug-Facilitated Sexual Assault by the Use of Flunitrazepam. J. Clin. Forensic Med. 2006, 13, 44-45.
3. Hackett, J.; Elian, A. A. Extraction and Analysis of Flunitrazepam=7- aminoflunitrazepam in Blood and Urine by LC-PDA and GC-MS Using Butyl SPE Columns. Forensic Sci. Int. 2006, 157, 156-162.
4. McCabe, S. E. Correlates of Nonmedical Use of Prescription Benzodiazepine Anxiolytics: Results from a National Survey of US. Drug Alcohol Depend. 2005, 79, 53-62.
5. San, L.; Tato, J.; Torrens, M.; Castillo, C.; Farre, M.; Cami, J. Flunitrazepam Consumption Among Heroin Addicts Admitted for In-Patient Detoxification. Drug Alcohol Depend. 1993, 32, 281-286.
6. Gelkopf, M.; Bleich, A.; Hayward, R.; Bodner, G.; Adelson, M. Characteristics of Benzodiazepine Abuse in Methadone Maintenance Treatment Patients: A 1 Year Prospective Study in an Israeli Clinic. Drug Alcohol Depend. 1999, 55, 63-68.
7. De Wet, C.; Reed, L.; Glasper, A.; Moran, M.; Bearn, J.; Gossop, M. Benzodiazepine Co-Dependence Exacerbates the Opiate Withdrawal Syndrome. Drug Alcohol Depend. 2004, 76, 31-35.
8. Rickert, V. I.; Wiemann, C. M.; Berenson, A. B. Prevalence, Patterns, and Correlates of Voluntary Flunitrazepam Use. Pediatrics. 1999, 103 (1), E6.
9. Scott-Ham, M.; Burton, F. C. Toxicological Findings of Alleged Drug-Facilitated Sexual Assault in the United Kingdom Over a 3-year Period. J. Clin. Forensic Med. 2005, 12, 175-186.
10. Elian, A. A. Detection of Low Levels of Flunitrazepam and Its Metabolites in Blood and Bloodstains. Forensic Sci. Int. 1999, 101, 107-111.
11. Terada, M.; Masui, S.; Hayashi, T.; Watanabe, R.; Inoue, H.; Iino, M.; Nakatome, M.; Matoba, R.; Shinozuka, T.; Murai, T.; Tanak, E.; Honda, K. Simultaneous Determination of Flunitrazepam and 7-aminoflunitrazepam in Human Serum by Ion Trap Gas Chromatography-Tandem Mass Spectrometry. Leg. Med. 2003, 5, S96-S100.
12. Cui, S.; Tan, S.; Ouyang, G.; Pawliszyn, J. Automated Polyvinylidene Difluoride Hollow Fiber Liquid-Phase Microextraction of Flunitrazepam in Plasma and Urine Samples for Gas Chromatography=Tandem Mass Spectrometry. J. Chromatogr. A 2009, 1216, 2241-2247.
13. Smink, B. E.; Brandsma, J. E.; Dijkhuizen, A.; Lusthof, K. J.; de Gier, J. J.; Egberts, A. C. G.; Uges, D. R. A. Quantitative Analysis of 33 Benzodiazepines, Metabolites and Benzodiazepine-Like Substances in Whole Blood by Liquid Chromatography-(Tandem) Mass Spectrometry. J. Chromatogr. B 2004, 811, 13-20.
14. Bugey, A.; Rudaz, S.; Staub, C. A Fast LC-APCI=MS Method for Analyzing Benzodiazepines in Whole Blood Using Monolithic Support. J. Chromatogr. B 2006, 832, 249-255.
15. Marchi, I.; Schappler, J.; Veuthey, J. L.; Rudaz, S. Development and Validation of a Liquid Chromatography-Atmospheric Pressure Photoionization-Mass Spectrometry Method for the Quantification of Alprazolam, Flunitrazepam, and Their Main Metabolites in Haemolysed Blood. J. Chromatogr. B 2009, 877, 2275-2283.
16. Berthault, F.; Kintz, P.; Mangin, P. Simultaneous High-Performance Liquid Chromatographic Analysis of Flunitrazepam and Four Metabolites in Serum. J. Chromatogr. B 1996, 685 (2), 383-387.
17. He, W.; Parissis, N. Simultaneous Determination of Flunitrazepam and Its Metabolites in Plasma and Urine by HPLC=DAD After Solid Phase Extraction. J. Pharm. Biomed. Anal. 1997, 16, 707-715.
18. El Mahjoub, A.; Staub, C. Simultaneous Determination of Benzodiazepines in Whole Blood or Serum by HPLC=DAD with a Semi-Micro Column. J. Pharm. Biomed. Anal. 2000, 23, 447-458.
19. Bugey, A.; Staub, C. Rapid Analysis of Benzodiazepines in Whole Blood by High-Performance Liquid Chromatography: Use of a Monolithic Column. J. Pharm. Biomed. Anal. 2004, 35, 555-562.
20. Bastos Borges, K.; Freire, E. F.; Martins, I.; Bastos De Siqueira, M. E. P. Simultaneous Determination of Multibenzodiazepines by HPLC=UV: Investigation of Liquid-Liquid and Solid-Phase Extractions in Human Plasma. Talanta 2009, 78, 233-241.
21. Benhamou-Batut, F.; Demotes-Mainard, F.; Labat, L.; Vincon, G.; Bannwarth, B. Determination of Flunitrazepam in Plasma by Liquid Chromatography. J. Pharm. Biomed. Anal. 1994, 12 (7), 931-936.
22. Kiss, B.; Popa, D. S.; Bojita, M.; Loghin, F. Development and Validation of a High-Performance Liquid Chromatography Method with Ultraviolet Detection for the Determination of Flunitrazepam in Human Plasma. Rev. Chim.-Bucharest 2008, 59 (12), 1301-1304.
23. Vlase, L.; Kiss, B.; Loghin, F.; Leucuta, S. E. Determination of Flunitrazepam in Human Plasma and Urine by HPLC with Mass Spectrometry Detection. J. Liq. Chromatogr. Rel. Technol. 2008, 31 (16), 2442-2454.
24. Moffat, A. C.; Osselton, M. D.; Widdop, B. (Eds.) Clarkes Analysis of Drugs and Poisons. 3rd ed.; Pharmaceutical Press: London, UK, 2004; Vols.775-776; pp 1044-1045.
25. U.S. Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research. Guidance for Industry. Bioavailability and Bioequivalence Studies for Orally Administered Drug Products-General Considerations; Rockville, MD, USA, March 2003, http://www.fda. gov/cder/guidance/index.htm
26. U.S. Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research. Guidance for Industry-Bioanalytical Method Validation; Rockville, MD, USA, May 2001.