Cloning and characterization of an endo-β-1,3(4)glucanase and an aspartic protease from Phaffia rhodozyma cbs 6938

Applied Microbiology and Biotechnology

Volumn 51, Issue 2, 1999, Pages 215-222

  Bang, M L ^a,c   Villadsen, I ^b   Sandal, T ^a  

^a NOVO NORDISK A S   (Denmark)

^b TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^c EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARTIC PROTEINASE; CELLULASE; COMPLEMENTARY DNA; PROTEIN PRECURSOR; SIGNAL PEPTIDE;

ARTICLE; DNA LIBRARY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME RELEASE; ISOELECTRIC POINT; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PHAFFIA RHODOZYMA; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE;

PHAFFIA RHODOZYMA; SACCHAROMYCES CEREVISIAE;

EID: 0033026416     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530051384     Document Type: Article

References (23)

1. An G-H, Bielich J, Auerbach R, Johnson EA (1991) Isolation and characterization of carotenoid hyperproducing mutants of yeast by flow cytometry and cell sorting. Biotechnology 9: 70-73
2. Becker DM, Guarente L (1991) High efficiency transformation of yeast by electroporation. Methods Enzymol 194: 182-187
3. Christgau S, Schierbeck, H, Aanstoot HJ, Aagard L, Begley K, Kofod H, Hejnæs K, Bækkeskov S (1991) Pancreatic β-cells express two autoantigenic forms of glutamic acid decarboxylase, a 65-kDa hydrophilic form and a 64-kDa amphiphilic form which can be both membrane-bound and soluble. J Biol Chem 266: 21 157-21 264
4. Christgau S, Sandal T, Kofod LV, Dalbøge H (1995) Expression cloning, purification and characterization of a β-1,4-galactanase from Aspergillus aculateus. Curr Genet 27: 135-141
5. Creighton TE (1993) Proteins. Structure and molecular properties, 2nd edn. Freeman, San Francisco
6. Dalbøge H, Heldt-Hansen HP (1994) A novel method for efficient expression cloning of fungal enzyme genes. Mol Gen Genet 243: 253-260
7. Devereux J, Haeberli P, Smithies O (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 11/ 12: 387-395
8. Draborg H, Kauppinen S, Dalbøge H, Christgau S (1995) Molecular cloning and expression in S. cerevisiae of two exochitinases from Trichoderma harzianum. Biochem Mol Biol Int 36: 781-791
9. Fuller RS, Brake AJ, Thorner J (1989) Intracellular targeting and structural conservation of a prohormone endoprotease. Science 246: 482-486
10. Hazel HB van den, Kielland-Brandt MC, Winther JR (1992) Autoactivation of proteinase A initiates activation of yeast vacuolar zymogens. Eur J Biochem 207: 277-283
11. Kofod LV, Kauppinen S, Christgau S, Andersen LN, Heldt-Hansen KP, Dörreich K, Dalbøge H (1994) Cloning and characterization of two structurally and functionally divergent rhamnogalacturonases from Aspergillus aculateus. J Biol Chem 269: 29 182-29 189
12. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685
13. Matsudaira PT (ed) (1989) A practical guide to protein and peptide purification of microsequencing. Academic Press Inc., San Diego
14. Miller MW, Yoneyama M, Soneda M (1976) Phaffia, a new yeast genus in the Deuteromycotina (Blastomyces) Int J Syst Bacteriol 26: 286-291
15. Nakamura Y, Wada K, Wada Y, Doi H, Kanaya S, Gojobori Y, Ikemura T (1996) Codon usage tabulated from the international DNA sequence databases. Nucleic Acids Res 24: 214-215
16. Nielsen H, Engelbrecht J, Brunak S, Heijne G von (1997) Identification of prokaryotic and eucaryotic signal peptide and prediction of their cleavage sites. Protein Eng 10: 1-6
17. Phaff HJ, Miller MW, Yoneyama M, Soneda M (1972) A comparative study of the yeast florae associated with trees on the Japanese Islands and on the West Coasts of North America. In: Terui G (ed) Proceedings of the 4th IFS: Fermentation technology today, Kyoto. Society of Fermentation Technology, Osaka, pp 759-774
18. Rich DH (1986) Inhibitors of aspartic proteases. Barrett AJ, Salvesen G (eds) Proteinase inhibitors. Elsevier, Amsterdam New York
19. Rosenberg AH, Goldman E, Dunn JJ, Studier FW, Zubay G (1993) Effects of consecutive AGG codons on translation in Escherichia coli, demonstrated with a versatile codon test system. J Bacteriol 75: 716-722
20. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-termination inhibitors. Proc Natl Acad Sci USA 74: 5463-5467
21. Sherman F (1991) Getting started with yeast. Methods Enzymol 194: 3-21
22. Thompson J, Higgins D, Gibson T (1994) CLUSTAL W:improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673-4680
23. Webb EC (1992) Enzyme nomenclature. Recommendation of the nomenclature committee of the international union of biochemistry and molecular biology on the nomenclature and classification of enzymes. The Executive Committee of the International Union of Biochemistry and Molecular Biology. Academic Press, New York