Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium

Applied Microbiology and Biotechnology

Volumn 88, Issue 2, 2010, Pages 529-539

  Stammen, Simon ^a   Schuller, Franziska ^b   Dietrich, Sylvia ^a   Gamer, Martin ^c   Biedendieck, Rebekka ^d   Jahn, Dieter ^a  

^a TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

^b HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^c UNIVERSITY OF LEIPZIG   (Germany)

^d UNIVERSITY OF KENT   (United Kingdom)

Author keywords

Bacillus megaterium; DNA dependant RNA polymerase; Escherichia coli phage K1E; In vitro transcription; Protein production

Indexed keywords

BACILLUS MEGATERIUM; IN-VITRO; IN-VIVO; MAGNESIUM CHLORIDES; MEGATERIUM; PLASMID DNA; PROMOTER REGION; PROTEIN PRODUCTION; REPORTER PROTEIN; RNA POLYMERASE; RNA SYNTHESIS; SHAKING-FLASK CULTURE; SPERMIDINE;

AFFINITY CHROMATOGRAPHY; BACTERIOLOGY; CHLORINE COMPOUNDS; CLONING; DNA; ESCHERICHIA COLI; GENES; MAGNESIUM; POLYMERS; PROTEINS; PURIFICATION; TRANSCRIPTION;

RNA;

BACTERIAL PROTEIN; BACTERIAL RNA; DNA DIRECTED RNA POLYMERASE; MAGNESIUM CHLORIDE; PLASMID DNA; SPERMIDINE;

BACTERIOPHAGE; CHROMATOGRAPHY; COLIFORM BACTERIUM; GENETIC ANALYSIS; GENOME; NUCLEIC ACID; PLASMID; PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; BACILLUS MEGATERIUM; BACTERIAL GENOME; BACTERIOPHAGE; BACTERIOPHAGE K1E; BACTERIUM CULTURE; ENVIRONMENTAL TEMPERATURE; ESCHERICHIA COLI; GENE SEQUENCE; GENETIC TRANSCRIPTION; IN VITRO STUDY; IN VIVO STUDY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PLASMID; PROMOTER REGION; RNA SYNTHESIS;

BACILLUS MEGATERIUM; COLIPHAGES; DNA-DIRECTED RNA POLYMERASES; RECOMBINANT PROTEINS; RNA; TEMPLATES, GENETIC; VIRAL PROTEINS;

BACILLUS MEGATERIUM; ESCHERICHIA COLI;

EID: 77956229130     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-010-2732-y     Document Type: Article

References (39)

1. Barg H, Malten M, Jahn M, Jahn D (2005) Protein and vitamin production in Bacillus megaterium. In: Barredo EL (ed) Microbial processes and products, 1st edn. Humana press, Totowa, NJ, pp 165-184
2. R Biedendieck Y Yang WD Deckwer M Malten D Jahn 2007 Plasmid system for the intracellular production and purification of affinity-tagged proteins in Bacillus megaterium Biotechnol Bioeng 96 525 537 10.1002/bit.21145 1:CAS:528:DC%2BD2sXhtVajtLk%3D
3. ET Butler MJ Chamberlin 1982 Bacteriophage SP6-specific RNA polymerase I. Isolation and characterization of the enzyme J Biol Chem 257 5772 5778 1:CAS:528:DyaL38XktValtLg%3D
4. MG Carter AA Sharov V VanBuren DB Dudekula CE Carmack C Nelson MS Ko 2005 Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray Genome Biol 6 R61 10.1186/gb-2005-6-7-r61
5. PR Chakraborty RA Salvo HK Majumder U Maitra 1977 Further characterization of bacteriophage T3-induced ribonucleic acid polymerase. Studies on the size of in vitro transcripts and interaction of T3 RNA polymerase with T3 DNA J Biol Chem 252 6485 6493 1:CAS:528:DyaE2sXlsVOqsrc%3D
6. M Chamberlin J Ring 1973 Characterization of T7-specific ribonucleic acid polymerase. 1. General properties of the enzymatic reaction and the template specificity of the enzyme J Biol Chem 248 2235 2244 1:CAS:528:DyaE3sXhsVCltL4%3D
7. Z Chen TD Schneider 2005 Information theory based T7-like promoter models: classification of bacteriophages and differential evolution of promoters and their polymerases Nucleic Acids Res 33 6172 6187 10.1093/nar/gki915 1:CAS:528:DC%2BD2MXht1entLjM
8. B Conrad RS Savchenko R Breves J Hofemeister 1996 A T7 promoter-specific, inducible protein expression system for Bacillus subtilis Mol Gen Genet 250 230 236 1:CAS:528:DyaK28XhsFOktbw%3D
9. GE Crooks G Hon JM Chandonia SE Brenner 2004 WebLogo: a sequence logo generator Genome Res 14 1188 1190 10.1101/gr.849004 1:CAS:528: DC%2BD2cXkvFGht7Y%3D
10. D Dubnau R Losick 2006 Bistability in bacteria Mol Microbiol 61 564 572 10.1111/j.1365-2958.2006.05249.x 1:CAS:528:DC%2BD28XotVequro%3D
11. TR Fuerst EG Niles FW Studier B Moss 1986 Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase Proc Natl Acad Sci USA 83 8122 8126 10.1073/pnas.83.21.8122 1:CAS:528:DyaL2sXhtlak
12. M Gamer D Frode R Biedendieck S Stammen D Jahn 2009 A T7 RNA polymerase-dependent gene expression system for Bacillus megaterium Appl Microbiol Biotechnol 82 1195 1203 10.1007/s00253-009-1952-5 1:CAS:528: DC%2BD1MXjvFeisrc%3D
13. M Golomb M Chamberlin 1974 Characterization of T7-specific ribonucleic acid polymeraseIV. Resolution of the major in vitro transcripts by gel electrophoresis J Biol Chem 249 2858 2863 1:CAS:528:DyaE2cXksVKrs70%3D
14. A Grote K Hiller M Scheer R Münch B Nortemann DC Hempel D Jahn 2005 JCat: a novel tool to adapt codon usage of a target gene to its potential expression host Nucleic Acids Res 33 W526 W531 10.1093/nar/gki376 1:CAS:528:DC%2BD2MXlslyrtLc%3D
15. HA Hong JM Huang R Khaneja LV Hiep MC Urdaci SM Cutting 2008 The safety of Bacillus subtilis and Bacillus indicus as food probiotics J Appl Microbiol 105 510 520 10.1111/j.1365-2672.2008.03773.x 1:CAS:528:DC%2BD1cXhtVCqtL%2FN
16. ED Jorgensen RK Durbin SS Risman WT McAllister 1991 Specific contacts between the bacteriophage T3, T7, and SP6 RNA polymerases and their promoters J Biol Chem 266 645 651 1:CAS:528:DyaK3MXnslKitg%3D%3D
17. R Lavigne D Seto P Mahadevan HW Ackermann AM Kropinski 2008 Unifying classical and molecular taxonomic classification: analysis of the Podoviridae using BLASTP-based tools Res Microbiol 159 406 414 10.1016/j.resmic.2008.03.005 1:CAS:528:DC%2BD1cXhtVSiu7%2FL
18. Y Lobet MG Peacock W Cieplak Jr 1989 Frame-shift mutation in the lacZ gene of certain commercially available pUC18 plasmids Nucleic Acids Res 17 4897 10.1093/nar/17.12.4897 1:CAS:528:DyaL1MXkslWquro%3D
19. McAllister WT (1991) Plasmid for the overproduction of bacteriophage T3 RNA polymerase, transcription vectors that carry a promoter recognized by its polymerase, gene coding for T3 RNA polymerase and application of these plasmids. US Patent 5,037,745 University of Medicine and Dentistry of New Jersey, USA
20. DA Melton PA Krieg MR Rebagliati T Maniatis K Zinn MR Green 1984 Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter Nucleic Acids Res 12 7035 7056 10.1093/nar/12.18.7035 1:CAS:528:DyaL2cXmt1Khtrs%3D
21. JF Milligan DR Groebe GW Witherell OC Uhlenbeck 1987 Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates Nucleic Acids Res 15 8783 8798 10.1093/nar/15.21.8783 1:CAS:528:DyaL1cXntFKhuw%3D%3D
22. R Münch K Hiller A Grote M Scheer J Klein M Schobert D Jahn 2005 Virtual Footprint and PRODORIC: an integrative framework for regulon prediction in prokaryotes Bioinformatics 21 4187 4189 10.1093/bioinformatics/bti635
23. Paschal BM, McReynolds LA, Noren CJ, Nichols NM (2008) RNA polymerases. Curr Protoc Mol Biol Chapter 3:Unit3 8
24. A Patkar N Vijayasankaran DW Urry F Srienc 2002 Flow cytometry as a useful tool for process development: rapid evaluation of expression systems J Biotechnol 93 217 229 10.1016/S0168-1656(01)00399-6 1:CAS:528:DC%2BD38XhvVej
25. T Rygus W Hillen 1991 Inducible high-level expression of heterologous genes in Bacillus megaterium using the regulatory elements of the xylose-utilization operon Appl Microbiol Biotechnol 35 594 599 10.1007/BF00169622 1:CAS:528:DyaK3MXlsFansLo%3D
26. H Sagawa A Ohshima I Kato 1996 A tightly regulated expression system in Escherichia coli with SP6 RNA polymerase Gene 168 37 41 10.1016/0378-1119(95) 00644-3 1:CAS:528:DyaK28XhtlWgurs%3D
27. Sambrook J, Fritsch EF, Maniatis T (2001) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory Press, New York
28. TD Schneider RM Stephens 1990 Sequence logos: a new way to display consensus sequences Nucleic Acids Res 18 6097 6100 10.1093/nar/18.20.6097 1:CAS:528:DyaK3MXitVGmuw%3D%3D
29. D Scholl J Kieleczawa P Kemp J Rush CC Richardson C Merril S Adhya IJ Molineux 2004 Genomic analysis of bacteriophages SP6 and K1-5, an estranged subgroup of the T7 supergroup J Mol Biol 335 1151 1171 10.1016/j.jmb.2003.11.035 1:CAS:528:DC%2BD2cXjtVChug%3D%3D
30. Schweizer HP, Klassen TR, Hoang T (1996) Improved methods for gene analysis and expression in Pseudomonas. In: Nakazawa T, Haas D, Silver S (eds) Molecular biology of pseudomonads, 1st edn. ASM Press, Washington, DC, pp 229-237
31. Stammen S, Müller BK, Korneli C, Biedendieck R, Gamer M, Franco-Lara E, Jahn D (2010) High yield intra- and extracellular protein production using Bacillus megaterium. Appl Environ Microbiol 76:4037-4046
32. Studier FW, Davanloo P, Rosenberg AH, Moffatt BA, Dunn JJ (1999) Cloning and expression of the gene for bacteriophage T7 RNA polymerase. US Patent 5,869,320 Brookhaven Science Associates LLC, Upton, NY, USA
33. K Stummeyer D Schwarzer H Claus U Vogel R Gerardy-Schahn M Mühlenhoff 2006 Evolution of bacteriophages infecting encapsulated bacteria: lessons from Escherichia coli K1-specific phages Mol Microbiol 60 1123 1135 10.1111/j.1365-2958.2006.05173.x 1:CAS:528:DC%2BD28XmtV2htrk%3D
34. WT Stump KB Hall 1993 SP6 RNA polymerase efficiently synthesizes RNA from short double-stranded DNA templates Nucleic Acids Res 21 5480 5484 10.1093/nar/21.23.5480 1:CAS:528:DyaK2cXks1ymtA%3D%3D
35. S Tabor CC Richardson 1985 A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes Proc Natl Acad Sci USA 82 1074 1078 10.1073/pnas.82.4.1074 1:CAS:528:DyaL2MXhsVCis7s%3D
36. PS Vary 1994 Prime time for Bacillus megaterium Microbiology 140 Pt 5 1001 1013 10.1099/13500872-140-5-1001 1:CAS:528:DyaK2cXktVCmtb0%3D
37. PS Vary R Biedendieck T Fuerch F Meinhardt M Rohde WD Deckwer D Jahn 2007 Bacillus megaterium-from simple soil bacterium to industrial protein production host Appl Microbiol Biotechnol 76 957 967 10.1007/s00253-007-1089-3 1:CAS:528:DC%2BD2sXhtVCns77E
38. KD Wittchen F Meinhardt 1995 Inactivation of the major extracellular protease from Bacillus megaterium DSM319 by gene replacement Appl Microbiol Biotechnol 42 871 877 10.1007/BF00191184 1:CAS:528:DyaK2MXkvV2jt7g%3D
39. Y Yang M Malten A Grote D Jahn WD Deckwer 2007 Codon optimized Thermobifida fusca hydrolase secreted by Bacillus megaterium Biotechnol Bioeng 96 780 794 10.1002/bit.21167 1:CAS:528:DC%2BD2sXitVertbo%3D