Tubulin α-6 chain is a stably expressed reference gene in archival samples of normal oral tissue and oral squamous cell carcinoma

Experimental and Therapeutic Medicine

Volumn 1, Issue 3, 2010, Pages 419-423

  Rentoft, Matild ^a   Hultin, Sara ^a   Coates, Philip John ^c   Laurell, Göran ^b   Nylander, Karin ^a  

^a UMEÅ UNIVERSITY   (Sweden)

^b UMEÅ UNIVERSITY HOSPITAL   (Sweden)

^c UNIVERSITY OF DUNDEE   (United Kingdom)

Author keywords

Oral squamous cell carcinoma; Quantitative real time PCR; Reference gene; Tubulin 6 chain

Indexed keywords

ALPHA TUBULIN; FORMALDEHYDE; RIBOSOME PROTEIN; TUBULIN ALPHA 6 CHAIN; UNCLASSIFIED DRUG;

ARTICLE; CLINICAL ARTICLE; COMPUTER PROGRAM; CONTROLLED STUDY; GENE EXPRESSION; GINGIVAL BIOPSY; HUMAN; HUMAN TISSUE; MOUTH CARCINOMA; NUCLEOTIDE SEQUENCE; PALATE; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; TONGUE;

EID: 77956198713     PISSN: 17920981     EISSN: 17921015     Source Type: Journal    
DOI: 10.3892/etm_00000065     Document Type: Article

References (29)

1. Shibuya K, Mathers CD, Boschi-Pinto C, Lopez AD and Murray CJ: Global and regional estimates of cancer mortality and incidence by site. II. Results for the global burden of disease 2000. BMC Cancer 2: 37, 2002.
2. Geisler SA and Olshan AF: GSTM1, GSTT1 and the risk of squamous cell carcinoma of the head and neck: a mini-HuGE review. Am J Epidemiol 154: 95-105, 2001.
3. Rautava J, Luukkaa M, Heikinheimo K, Alin J, Grenman R and Happonen RP: Squamous cell carcinomas arising from different types of oral epithelia differ in their tumor and patient characteristics and survival. Oral Oncol 43: 911-919, 2007.
4. Bettendorf O, Piffko J and Bankfalvi A: Prognostic and predictive factors in oral squamous cell cancer: important tools for planning individual therapy? Oral Oncol 40: 110-119, 2004.
5. Culliney B, Birhan A, Young AV, Choi W, Shulimovich M and Blum RH: Management of locally advanced or unresectable head and neck cancer. Oncology 22: 1152-1161, 2008.
6. Lothaire P, de Azambuja E, Dequanter D, et al: Molecular markers of head and neck squamous cell carcinoma: promising signs in need of prospective evaluation. Head Neck 28: 256-269, 2006.
7. Shirai K and O'Brien PE: Molecular targets in squamous cell carcinoma of the head and neck. Curr Treat Options Oncol 8: 239-251, 2007.
8. Ginzinger DG: Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream. Exp Hematol 30: 503-512, 2002.
9. Huggett J, Dheda K, Bustin S and Zumla A: Real-time RT-PCR normalisation; strategies and considerations. Genes Immun 6: 279-284, 2005.
10. Vandesompele J, Kubista M and Pfaffl M: Reference gene validation software for improved normalization. In: Real-Time PCR: Current Technology and Applications. 1st edition. Logan J, Edwards K and Saunders N (eds). Caister Academic Press, London, pp47-64, 2009.
11. Ruan W and Lai M: Actin, a reliable marker of internal control? Clin Chim Acta 385: 1-5, 2007.
12. Masuda N, Ohnishi T, Kawamoto S, Monden M and Okubo K: Analysis of chemical modification of RNA from formalin-fixed samples and optimization of molecular biology applications for such samples. Nucleic Acids Res 27: 4436-4443, 1999.
13. Von Ahlfen S, Missel A, Bendrat K and Schlumpberger M: Determinants of RNA quality from FFPE samples. PLoS ONE 2: e1261, 2007.
14. Clark-Langone KM, Wu JY, Sangli C, et al: Biomarker discovery for colon cancer using a 761 gene RTRT-PCRPCRPCR assay. BMC Genomics 8: 279, 2007.
15. Penland SK, Keku TO, Torrice C, et al: RNA expression analysis of formalin-fixed paraffin-embedded tumors. Lab Invest 87: 383-391, 2007.
16. Rozen S and Skaletsky H: Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol 132: 365-386, 2000.
17. Larionov A, Krause A and Miller W: A standard curve based method for relative real time PCR data processing. BMC Bioinformatics 6: 62, 2005.
18. Andersen CL, Jensen JL and Orntoft TF: Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res 64: 5245-5250, 2004.
19. Vandesompele J, De Preter K, Pattyn F, et al: Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3: RESEARCH0034, 2002).
20. De Jonge HJ, Fehrmann RS, de Bont ES, et al: Evidence based selection of housekeeping genes. PLoS ONE 2: e898, 2007.
21. Drury S, Anderson H and Dowsett M: Selection of reference genes for normalization of qRT-PCR data derived from FFPE breast tumors. Diagn Mol Pathol 18: 103-107, 2009.
22. Perez-Novo CACA, Claeys C, Speleman F, van Cauwenberge P P, Bachert C and Vandesompele J: Impact of RNA quality on reference gene expression stability. Biotechniques 39: 52, 54, 56, 2005.
23. Hibbs K, Skubitz KM, Pambuccian SE, et al: Differential gene expression in ovarian carcinoma: identification of potential biomarkers. Am J Pathol 165: 397-414, 2004.
24. Yao R, Lopez-Beltran A, Maclennan GT, Montironi R, Eble JN and Cheng L: Expression of S100 protein family members in the pathogenesis of bladder tumors. Anticancer Res 27: 3051-3058, 2007.
25. Campos MS, Rodini CO, Pinto-Junior DS and Nunes FD: GAPD and tubulin are suitable internal controls for qPCR analysis of oral squamous cell carcinoma cell lines. Oral Oncol 45: 121-126, 2009.
26. Rentoft M, Laurell G, Coates P, Sjöström B and Nylander K: Gene expression profiling of archival tongue squamous cell carcinomas provides sub-classification based on DNA repair genes. Int J Oncol 35: 1321-1330, 2009.
27. Cicinnati VR, Shen Q, Sotiropoulos GC, Radtke A, Gerken G and Beckebaum S: Validation of putative reference genes for gene expression studies in human hepatocellular carcinoma using real-time quantitative RT-PCR. BMC Cancer 8: 350, 2008.
28. Lallemant B, Evrard A, Combescure C, et al: Reference gene selection for head and neck squamous cell carcinoma gene expression studies. BMC Mol Biol 10: 78, 2009.
29. Lyng MB, Laenkholm AV, Pallisgaard N and Ditzel HJ: Identification of genes for normalization of real-time RT-PCR data in breast carcinomas. BMC Cancer 8: 20, 2008.