Determinants of RNA quality from FFPE samples

PLoS ONE

Volumn 2, Issue 12, 2007, Pages

  von Ahlfen, Silke ^a   Missel, Andreas ^a   Bendrat, Klaus ^b   Schlumpberger, Martin ^a  

^a QIAGEN GMBH   (Germany)

^b Gemeinschaftspraxis für Pathologie Niendorf und Hamper   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; FORMALDEHYDE; PARAFFIN; RNA;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CLINICAL PRACTICE; CONTROLLED STUDY; DNA SYNTHESIS; EMBEDDING; GENE EXPRESSION PROFILING; HISTOPATHOLOGY; MATERIALS HANDLING; MICROARRAY ANALYSIS; MOLECULAR BIOLOGY; NONHUMAN; NUCLEOTIDE SEQUENCE; QUALITY CONTROL; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STORAGE TEMPERATURE; TIME; TISSUE FIXATION; TISSUE PRESERVATION; ANIMAL; ISOLATION AND PURIFICATION; LABORATORY DIAGNOSIS; STANDARD; WISTAR RAT;

ANIMALS; FORMALDEHYDE; PARAFFIN EMBEDDING; RATS; RATS, WISTAR; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; SPECIMEN HANDLING; TISSUE FIXATION;

EID: 42149143085     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0001261     Document Type: Article

References (12)

1. Lewis F, Maughan NJ, Smith V, Hillan K, Quirke P (2001) Unlocking the archive-gene expression in paraffin-embedded tissue. J. Pathol. 195: 66-71.
2. Masuda N, Ohnishi T, Kawamoto S, Monden M, Okubo K (1999) Analysis of chemical modification of RNA from formalin-fixed samples and optimization of molecular biology applications for such samples. Nucleic Acids Res. 27: 4436-4443.
3. Bresters D, Schipper MEI, Reesink HW, Boeser-Nunnink BDM, Cuypers HTM (1994) The Duration of Fixation Influences the Yield of HCV cDNA-PCR Products from Formalin-Fixed, Paraffin-Embedded Tissue. J. Virol. Methods 48: 267-272.
4. Macabeo-Ong M, Ginzinger DG, Dekker N, McMillan A, Regezi JA, et al. (2002) Effect of duration of Fixation on quantitative reverse transcription polymerase chain reaction analyses. Mod. Pathol. 15: 979-987.
5. Bhudevi B, Weinstock D (2003) Detection of Bovine Viral Diarrhea Virus in Formalin Fixed Paraffin embedded Tissue Sections by Real Time RT-PCR. J. Virol. Methods. 109: 25-30.
6. Cronin M, Pho M, Dutta D, Stephans JC, Shak S, Kiefer MC, Esteban JM, Baker JB (2004) Measurement of Gene Expression in Archival Paraffin-Embedded Tissues. Am. J. Pathol. 164: 35-42.
7. Werner M, Chott A, Fabiano A, Battifora H (2000) Effect of formalin tissue fixation and processing on immunohistochemistry. Am J Surg Pathol. 24: 1016-9.
8. Imbeaud S, Graudens E, Boulanger V, Barlet X, Zaborski P, et al. (2005) Towards standardization of RNA quality assessment using user-independent classifiers of microcapillary electrophoresis traces. Nucleic Acids Res. 33: e56.
9. Krafft AE, Duncan BW, Bijwaard KE, Taubenberger JK, Lichy JH (1997) Optimization of the Isolation and Amplification of RNA From Formalin-fixed, Paraffin-embedded Tissue: The Armed Forces Institute of Pathology Experience and Literature Review. Mol Diagn. 2: 217-230.
10. Dempster WT (1960) Rates of penetration of fixing fluids. Am J Anat. 107: 59-72.
11. Srinivasan M, Sedmak D, Jewell S (2002) Effect of fixatives and tissue processing on the content and integrity of nucleic acids. Am J Pathol. 161: 1961-71.
12. Kraus E, Kitz HH, Femfert UF (1976) The specificity of proteinase K against oxidized insulin B chain. Hoppe-Seyler's Z. Physiol. Chem. 357: 233-237.