Molecular cloning and characterization of phenylalanine ammonia-lyase, cinnamate 4-hydroxylase and genes involved in flavone biosynthesis in Scutellaria baicalensis

Bioresource Technology

Volumn 101, Issue 24, 2010, Pages 9715-9722

  Xu, Hui ^a   Park, Nam Il ^a   Li, Xiaohua ^a   Kim, Yong Kyoung ^a   Lee, Sook Young ^b   Park, Sang Un ^a  

^a Chungnam National University   (South Korea)

^b Chosun University   (South Korea)

Author keywords

Cinnamate 4 hydroxylase; Flavone; Phenylalanine ammonia lyase; Scutellaria baicalensis

Indexed keywords

BAICALEIN; BAICALIN; CDNA ENDS; CHALCONE SYNTHASE; CINNAMATE 4-HYDROXYLASE; EXPRESSION LEVELS; FLAVONE; ISOFORMS; METHYL JASMONATE; MOLECULAR CLONING; PHENYLALANINE AMMONIA-LYASE; PLANT SPECIES; SCUTELLARIA BAICALENSIS; STRESS FACTORS; SUSPENSION CELL;

AMINO ACIDS; AMMONIA; AMPLIFICATION; BIOCHEMISTRY; BIOSYNTHESIS; CLONING; ENCODING (SYMBOLS); GENE ENCODING;

PLANTS (BOTANY);

ACETYL COENZYME A SYNTHETASE; BAICALEIN; BAICALIN; CHALCONE SYNTHASE; CINNAMATE 4 MONOOXYGENASE; COMPLEMENTARY DNA; JASMONIC ACID METHYL ESTER; PHENYLALANINE AMMONIA LYASE; RNA;

BIOTIC FACTOR; CLONAL GROWTH; DICOTYLEDON; ENZYME ACTIVITY; GENE; MEDICINAL PLANT; MITOCHONDRIAL DNA;

ABIOTIC STRESS; ARTICLE; BIOSYNTHESIS; BIOTIC STRESS; CELL SUSPENSION; DNA ISOLATION; FLOWER; GENE AMPLIFICATION; GENE EXPRESSION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT LEAF; PLANT ROOT; PLANT STEM; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RECEPTOR UPREGULATION; RNA EXTRACTION; SCUTELLARIA BAICALENSIS;

AMINO ACID SEQUENCE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CLONING, MOLECULAR; FLAVANONES; FLAVONES; FLAVONOIDS; GENE EXPRESSION REGULATION, PLANT; GENES, PLANT; MOLECULAR SEQUENCE DATA; ORGAN SPECIFICITY; PHENYLALANINE AMMONIA-LYASE; SCUTELLARIA BAICALENSIS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; TIME FACTORS; TRANS-CINNAMATE 4-MONOOXYGENASE;

SCUTELLARIA BAICALENSIS;

EID: 77956171754     PISSN: 09608524     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biortech.2010.07.083     Document Type: Article

References (35)

1. Chapple C. Molecular-genetic analysis of plant cytochrome P450dependent monooxygenases. Annual Review of Plant Physiology and Plant Molecular Biology 1998, 49:311-343.
2. Chi Y.S., Lim H., Park H., Kim H.P. Effects of wogonin, a plant flavone from Scutellaria radix, on skin inflammation: in vivo regulation of inflammation-associated gene expression. Biochemical Pharmacology 2003, 66:1271-1278.
3. Creelman R.A., Tierney M.L., Mullet J.E. Jasmonic acid/methyl jasmonate accumulate in wounded soybean hypocotyls and modulate wound gene expression. Proceedings of the National Academy of Sciences of the United States of America 1992, 89:4938-4941.
4. Dixon R.A., Paiva N.L. Stress-induced phenylpropanoid metabolism. Plant Cell 1995, 7:1085-1097.
5. Gao S.L., Chen B.J., Zhu D.N. In vitro production and identification of autotetraploids of Scutellaria baicalensis. Plant Cell, Tissue and Organ Culture 2002, 70:289-293.
6. Gao Z., Yang X., Huang K., Xu H. Free-radical scavenging and mechanism study of flavonoids extracted from the radix of Scutellaria baicalensis Georgi. Applied Magnetic Resonance 2000, 19:35-44.
7. Georgiev M.I., Pavlov A.I., Bley T. Hairy root type plant in vitro systems as sources of bioactive substances. Applied Microbiology and Biotechnology 2007, 74:1175-1185.
8. Gundlach H., Muller M.J., Kutchan T.M., Zenk M.H. Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures. Proceedings of the National Academy of Sciences USA 1992, 89:2389-2393.
9. Guo Q., Zhao L., You Q., Yang Y., Gu H., Song G., Lu N., Xin J. Anti-hepatitis B virus activity of wogonin in vitro and in vivo. Antiviral Research 2007, 74:16-24.
10. Harakava R. Genes encoding enzymes of the lignin biosynthesis pathway in Eucalyptus. Genetics and Molecular Biology 2005, 28:601-607.
11. Hayashi H., Huang P., Inoue K. Up-regulation of soyasaponin biosynthesis by methyl jasmonate in cultured cells of Glycyrrhiza glabra. Plant Cell Physiology 2003, 44:404-411.
12. Horvath C.R., Martos P.A., Saxena P.K. Identification and quantification of eight flavones in root and shoot tissues of the medicinal plant Huang-qin (Scutellaria baicalensis Georgi) using high-performance liquid chromatography with diode array and mass spectrometric detection. Journal of Chromatography A 2005, 1062:199-207.
13. Hwang S. Baicalin production in transformed hairy root clones of Scutellaria baicalensis. Biotechnology and Bioprocess Engineering 2006, 11:105-109.
14. Junghans H., Dalkin K., Dixon R.A. Stress responses In alfalfa (Medicago-Sativa L). 15. Characterization and expression patterns of members of a subset of the chalcone synthase Multigene Family. Plant Molecular Biology 1993, 22:239-253.
15. Komaraiah P., Reddy G.V., Reddy P.S., Raghavendra A.S., Ramakrishna S.V., Reddanna P. Enhanced production of antimicrobial sesquiterpenes and lipoxygenase metabolites in elicitor-treated hairy root cultures of Solanum tuberosum. Biotechnology Letters 2003, 25:593-597.
16. Kovács G., Kuzovkina I.N., Szoke É., Kursinszki L. HPLC determination of flavonoids in hairy-root cultures of Scutellaria baicalensis Georgi. Chromatographia 2004, 60:S81-85.
17. Kuzovkina I.N., Guseva A.V., Kovács D., Szöke É., Vdovitchenko M.Y. Flavones in genetically transformed Scutellaria baicalensis roots and induction of their synthesis by elicitation with methyl jasmonate. Russian Journal of Plant Physiology 2005, 52:77-82.
18. Lau C.S., Carrier D.J., Beitle R.R., Bransby D.I., Howard L.R., Lay J.J.O., Liyanage R., Clausen E.C. Identification and quantification of glycoside flavonoids in the energy crop Albizia julibrissin. Bioresource Technology 2007, 98:429-435.
19. Li-Weber M. New therapeutic aspects of flavones: the anticancer properties of Scutellaria and its main active constituents Wogonin, Baicalein and Baicalin. Cancer Treatment Reviews 2009, 35:57-68.
20. Li H., Murch S., Saxena P. Thidiazuron-induced de novo shoot organogenesis on seedlings, etiolated hypocotyls and stem segments of Huang-qin. Plant Cell, Tissue and Organ Culture 2000, 62:169-173.
21. Liu R., Xu S., Li J., Hu Y., Lin Z. Expression profile of a PAL gene from Astragalus membranaceus var Mongholicus and its crucial role in flux into flavonoid biosynthesis. Plant Cell Reports 2006, 25:705-710.
22. Lu M.B., Wong H.L., Teng W.L. Effects of elicitation on the production of saponin in cell culture of Panax ginseng. Plant Cell Reports 2001, 20:674-677.
23. Martens S., Mithöfer A. Flavones and flavone synthases. Phytochemistry 2005, 66:2399-2407.
24. Murashige T., Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum 1962, 15:473-497.
25. Murch S.J., Rupasinghe H.P.V., Goodenowe D., Saxena P.K. A metabolomic analysis of medicinal diversity in Huang-qin (Scutellaria baicalensis Georgi) genotypes: discovery of novel compounds. Plant Cell Reports 2004, 23:419-425.
26. Palazon J., Cusido Rosa M., Bonfill M., Mallol A., Moyano E., Morales C., Pinol M.T. Elicitation of different Panax ginseng transformed root phenotypes for an improved ginsenoside production. Plant Physiology and Biochemistry 2003, 41:1019-1025.
27. Rahman L.u., Kouno H., Hashiguchi Y., Yamamoto H., Narbad A., Parr A., Walton N., Ikenaga T., Kitamura Y. HCHL expression in hairy roots of Beta vulgaris yields a high accumulation of p-hydroxybenzoic acid (pHBA) glucose ester, and linkage of pHBA into cell walls. Bioresource Technology 2009, 100:4836-4842.
28. Richard S., Lapointe G., Rutledge R.G., Seguin A. Induction of chalcone synthase expression in white spruce by wounding and jasmonate. Plant Cell Physiology 2000, 41:982-987.
29. Singh K., Kumar S., Rani A., Gulati A., Ahuja P. Phenylalanine ammonia-lyase (PAL) and cinnamate 4-hydroxylase (C4H) and catechins (flavan-3-ols) accumulation in tea. Functional & Integrative Genomics 2009, 9:125-134.
30. Son D., Lee P., Lee J., Kim H., Kim S.Y. Neuroprotective effect of wogonin in hippocampal slice culture exposed to oxygen and glucose deprivation. European Journal of Pharmacology 2004, 493:99-102.
31. Srivastava S., Srivastava A.K. Hairy root culture for mass-production of high-value secondary metabolites. Critical Reviews in Biotechnology 2007, 27:29-43.
32. Teutsch H.G., Hasenfratz M.P., Lesot A., Stoltz C., Garnier J.M., Jeltsch J.M., Durst F., Werch-Reichhart D. Isolation and sequence of a cDNA encoding the Jerusalem artichoke cinnamate-4-hydroxylase, a major plant cytochrome P450 involved in the general phenylpropanoid pathway. Proceedings of the National Academy of Sciences USA 1993, 90:4102-4106.
33. Weisshaar B., Jenkins G.I. Phenylpropanoid biosynthesis and its regulation. Current Opinion in Plant Biology 1998, 1:251-257.
34. Zhao Q., Wang J., Zou M.-J., Hu R., Zhao L., Qiang L., Rong J.-J., You Q.-D., Guo Q.-L. Wogonin potentiates the antitumor effects of low dose 5-fluorouracil against gastric cancer through induction of apoptosis by down-regulation of NF-kappaB and regulation of its metabolism. Toxicology Letters 2010, 197:201-210.
35. Zhou Y., Nagashima S., Hirotani M., Suzuki H., Yoshikawa T. Expression of the chalcone synthase gene in Scutellaria baicalensis hairy root cultures was unusually reduced by environmental stresses. Plant Biotechnology 2003, 20:207-214.