Expression of the Chalcone Synthase Gene in Scutellaria baicalensis Hairy Root Cultures Was Unusually Reduced by Environmental Stresses

Plant Biotechnology

Volumn 20, Issue 3, 2003, Pages 207-214

  Zhou, Yu ^a   Nagashima, Shigeyuki ^a   Hirotani, Masao ^a   Suzuki, Hideyuki ^a   Yoshikawa, Takafumi ^a  

^a KITASATO UNIVERSITY   (Japan)

Author keywords

chalcone synthase; environmental stresses; gene expression; hairy root cultures; Scutellaria baicalensis

Indexed keywords

EID: 77956179690     PISSN: 13424580     EISSN: 13476114     Source Type: Journal    
DOI: 10.5511/plantbiotechnology.20.207     Document Type: Article

References (30)

1. Ayabe, S., Udagawa, A., Furuya, T., 1988. NAD(P)H-dependent 6'-deoxychalcone synthase activity in Gly-cyrrhiza echinata cells induced by yeast extract. Arch. Biochem. Biophys., 261: 458-462.
2. Batschauer, A., Khmann, B., Schäfer, E., 1991. Cloning and characterization of a chalcone synthase gene from mustard and its light-dependent expression. Plant Mol. Biol., 16: 175-185.
3. Bell, E. A., 1981. The physiological role(s) of secondary (natural) products. In: E. E. Conn (Ed.): The Biochemistry of PlantS., Vol. 7, pp. 1-19, Academic Press, New York.
4. Benton, W. D., DaviS., R. W., 1977. Screening λgt recombinant clones by hybridization to single plaques in situ. Science, 196: 180-182.
5. Chappell, J., HahlbrocK., K., 1984. Transcription of plant defence genes in response to UV light or fungal elicitor. Nature, 311: 76-78.
6. Chiang Su New Medical College, 1977. Dictionary of Chinese Crude DrugS., pp. 2017-2021. Shanghai Scientific Technological Publishers, Shanghai (in Chinese). Chomczynski, P., Sacchi, N., 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem., 162: 156-159.
7. Christensen, A. B., Gregersen, P. L., Schroder, J., Collinge, D. B., 1998. A chalcone synthase with an unusual substrate preference is expressed in barley leaves in response to UV light and pathogen attack. Plant Mol. Biol., 37: 849-857.
8. Christie, J. M., JenkinS., G. I., 1996. Distinct UV-B and UV-A/blue light signal transduction pathways induce chalcone synthase gene expression in Arabidopsis cells. Plant Cell, 8: 1555-1567.
9. Falquet, L., Pagni, M., Bucher, P., Hulo, N., Sigrist, C. J., Hofmann, K., Bairoch, A., 2002. “The PROSITE database, its status in 2002”. Nucleic Acids Res., 30: 235-238.
10. Fliegmann, J., Schroder, G., Schanz, S., Britsch, L., Schroder, J., 1992. Molecular analysis of chalcone and dihy-dropinosylvin synthase from Scots pine (Pinus sylvestris), and differential regulation of these and related enzyme activities in stressed plants. Plant Mol. Biol., 18: 489-503.
11. Fuglevand, G., Jackson, J. A., JenkinS., G. I., 1996. UV-B, UV-A, and blue light signal transduction pathway interact synergetically to regulate chalcone synthase gene expression in Arabidopsis. Plant Cell, 8: 2347-2357.
12. Gamborg, O. L., Miller, R. A., Ojima, K., 1968. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res., 50: 151-158.
13. Gong, Z., Yamazaki, M., Sugiyama, M., Tanaka, Y., Saito, K., 1997. Cloning and molecular analysis of structural genes involved in anthocyanin biosynthesis and expressed in a forma-specific manner in Perilla frutescens. Plant Mol. Biol., 35: 915-927.
14. Grab, D., Loyal, R., Ebel, J., 1985. Elicitor-induced phytoalexin synthesis in soybean cells: changes in the activity of chalcone synthase mRNA and the total population of translatable mRNA. Arch. Biochem. Biophys., 243: 523-529.
15. HedricK., S. A., Bell, J. N., Boiler, T., Lamb, C. J., 1988. Chitinase cDNA cloning and mRNA induction by fungal elicitor, wounding, and infection. Plant Physiol., 86: 182-186.
16. Heller, W., Forkmann, G., 1988. Biosynthesis. In: Harborne, J. B. (Ed.): The Flavonoids: Advances in Research Since 1980, pp. 399-425. Chapman and Hall, New York.
17. Junghans, H., Dalkin, K., Dixon, R. A., 1993. Stress responses in alfalfa (Medicago saliva L.). 15. Characterization and expression pattern of members of a subset of the chalcone synthase multigene family. Plant Mol. Biol., 22: 239-253.
18. Kreuzaler, F., Ragg, H., Fautz, E., Kuhn, D. N., HahlbrocK., K., 1983. UV-induction of chalcone synthase mRNA in cell suspension cultures of Petroselinum hortense. Proc. Natl. Acad. Sci. U. S. A., 80: 2591-2593.
19. Liu, Q., Dixon, R. A., Mabry, T. J., 1993a. Additional flavonoids from elicitor-treated cell cultures of Cephalocereus senilis. Phytochemistry, 34: 167-170.
20. Liu, Q., MarkhaM., K. R., Pare, P. W., Dixon, R. A., Mabry, T. J., 1993b. Flavonoids from elicitor-treated cell suspension cultures of Cephalocereus senilis. Phytoche mistry, 32: 925-928.
21. Logemann, E., Tavernaro, A., Schulz, W., Somssich, I. E., Hahlbrock, K., 2000. UV light selectively coinduces supply pathway from primary metabolism and flavonoids secondary product formation in parsley. Proc. Natl. Acad. Sci. U. S. A., 97: 1903-1907.
22. Morita, H., Noguchi, H., Akiyama, T., Shibuya, M., Ebi-zuka, Y., 1998. Chalcone synthase from Scutellaria baicalensis. In: Ageta, H., et al. (Eds.): Towards Natural Medicine Research in the 21st Century, pp. 401-409. Elsevier Science, Tokyo.
23. Richard, S., Lapointe, G., Rutledge, R. G., Séguin, A., 2000. Induction of chalcone synthase expression in white spruce by wounding and jasmonate. Plant Cell Physiol., 41:982-987.
24. Ryder, T. B., Cramer, C. L., Bell, J. N., RobbinS., M. P., Dixon, R. A., Lamb, C. J., 1984. Elicitor rapidly induces chalcone synthase mRNA in Phaseolus vulgaris cells at the onset of the phytoalexin defense response. Proc. Natl. Acad. Sci. U. S. A., 81: 5724-5728.
25. Sallaud, C., El-TurK., J., Breda, C., Buffard, D., KozaK., I., Esnault., R., Kondorosi, A., 1995. Differential expression of cDNA coding for chalcone reductase, a key enzyme of the 5-deoxyflavonoid pathway, under various stress conditions in Medicago sativa. Plant Sci., 109: 179-190.
26. Sambrook, J., FritscH., E. F., ManiatiS., T., 1989, Molecular cloning, 2nd ed. Cold Spring Harbor Laboratory Press, Plainview, New York.
27. Schmelzer, E., Jahnen, W., HahlbrocK., K., 1988. In situ localization of light-induced chalcone synthase mRNA, chalcone synthase, and flavonoid end products in epidermal cells of parsley leaves. Proc. Natl. Acad. Sci. U. S. A., 85: 2989-2993.
28. Tang, W., Eisenbrand, G., 1992. Chinese drugs of plant origin, pp. 919-929. Springer, Berlin Heidelberg.
29. Tunen, A. J., Koes, R. E., Spelt, C. E., Krol, A. R., Stuitje, A. R., Mol, J. N. M., 1988. Cloning of the two chalcone flavanone isomerase genes from Petunia hybrida: coordinate, light-regulated and differential expression of flavonoid genes. EMBO J., 7: 1257-1236.
30. Zhou, Y., Hirotani, M., Yoshikawa, T., Furuya, T., 1997. Flavonoids and phenylethanoids from hairy root cultures of Scutellaria baicalensis. Phytochemistry, 44: 83-87.