Venom on ice: First insights into Antarctic octopus venoms

Toxicon

Volumn 56, Issue 6, 2010, Pages 897-913

  Undheim, E A B ^a,b   Georgieva, D N ^c   Thoen, H H ^a,b   Norman, J A ^b   Mork, J ^a   Betzel, C ^c   Fry, B G ^b  

^a NORWEGIAN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Norway)

^b UNIVERSITY OF MELBOURNE   (Australia)

^c UNIVERSITY OF HAMBURG   (Germany)

Author keywords

Adaptation; Antarctic; Enzyme; Octopoda; Venom

Indexed keywords

ACETYLCHOLINESTERASE; ALKALINE PHOSPHATASE; OCTOPUS VENOM; PHOSPHOLIPASE A2; TISSUE EXTRACT; UNCLASSIFIED DRUG; VENOM;

ADELIELEDONE POLYMORPHA; ANIMAL TISSUE; ANTARCTICA; ARTICLE; BEAK; CHEMICAL COMPOSITION; CIRROCTOPUS; CONTROLLED STUDY; DIET; ECOLOGY; ENZYME ACTIVITY; EVOLUTIONARY ADAPTATION; HEMOLYSIS; MEGALELEDONE SETEBOS; MORPHOLOGY; NONHUMAN; NUCLEOTIDE SEQUENCE; ORGAN SIZE; PARELEDONE AEQUIPAPILLAE; PARELEDONE TURQUETI; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN SECRETION; SALIVARY GLAND; STOMACH; TOXIC OCTOPUS;

ADAPTATION, PHYSIOLOGICAL; ALKALINE PHOSPHATASE; ANIMALS; ANTARCTIC REGIONS; CHOLINESTERASE INHIBITORS; COLD TEMPERATURE; ENVIRONMENT; ERYTHROCYTES; FEMALE; HEMOLYSIS; MALE; MOLLUSK VENOMS; OCTOPODIFORMES; PHENOTYPE; PHYLOGENY; SALIVARY GLANDS;

ADELIELEDONE POLYMORPHA; CIRRATA; CIRROCTOPUS; INCIRRATA; MEGALELEDONE; OCTOPODA; OCTOPODIDAE; OCTOPUS; PARELEDONE; PARELEDONE TURQUETI;

EID: 77956062486     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.toxicon.2010.06.013     Document Type: Article

References (119)

1. Aird S.D. Ophidian envenomation strategies and the role of purines. Toxicon 2002, 40:335-393.
2. Allcock A.L. On the confusion surrounding Pareledone charcoti (Joubin, 1905) (Cephalopoda: Octopodidae): endemic radiation in the Southern ocean. Zool. J. Linn. Soc. 2005, 143:75-108.
3. Allcock A.L., Brierley A.S., Thorpe J.P., Rodhouse P.G. Restricted gene flow and evolutionary divergence between geographically separated populations of the Antarctic octopus Pareledone turqueti. Mar. Biol. 1997, 129:97-102.
4. Allcock A.L., Piatkowski U., Rodhouse P., Thorpe J. A study on octopodids from the eastern Weddell Sea, Antarctica. Polar Biol. 2001, 24:832-838.
5. Allcock A.L., Hochberg F.G., Rodhouse P.G.K., Thorpe J.P. Adelieledone, a new genus of octopodid from the Southern Ocean. Antarct. Sci. 2003, 15:415-424.
6. Allcock A.L., Hochberg F.G., Stranks T.N. Re-evaluation of Graneledone setebos (Cephalopoda: Octopodidae) and allocation to the genus Megaleledone. J. Mar. Biolog. Assoc. U.K. 2003, 83:319-328.
7. Allcock A.L., Lockhart S., Ellingsen K., Mooi R., de Broyer C. Introduction to work at sea. Ber. Polar Meeresforsch 2003, 470:91-94.
8. Allcock A.L., Strugnell J., Prodöhl P., Piatkowski U., Vecchione M. A new species of Pareledone (Cephalopoda: Octopodidae) from Antarctic Peninsula waters. Polar Biol. 2007, 30:883-893.
9. Allcock A.L., Piertney S. Evolutionary relationships of Southern Ocean Octopodidae (Cephalopoda: Octopa) and a new diagnosis of Pareledone. Mar. Biol. 2002, 140:129-135.
10. Ambrose R.F. Food preferences, prey availability, and the diet of Octopus bimaculatus Verrill. J. Exp. Mar. Biol. Ecol. 1984, 77:29-44.
11. Ambrose R.F., Nelson B.V. Predation by Octopus vulgaris in the Mediterranean. Mar. Ecol. 1983, 4:251-261.
12. Berkov A., Rodríguez N., Centeno P. Convergent evolution in the antennae of a cerambycid beetle, Onychocerus albitarsis, and the sting of a scorpion. Naturwissenschaften 2008, 95:257-261.
13. Boevé J.-L., Kuhn-Nentwig L., Keller S., Nentwig W. Quantity and quality of venom released by a spider (Cupiennius salei, Ctenidae). Toxicon 1995, 33:1347-1357.
14. Boucaud-Camou E., Roper C.F.E. Digestive enzymes in paralarval cephalopods. Bull. Mar. Sci. 1995, 57:313-327.
15. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72:248-254.
16. 2 from the Antarctic rock cod Trematomus bernacchii. FEBS J. 2007, 274:6152-6166.
17. Cavazzoni E., Lister B., Sargent P., Schibler A. Blue-ringed octopus (Hapalochlaena sp.) envenomation of a 4-year-old boy: a case report. Clin. Toxicol. 2008, 46:760-761.
18. Chen D., Kini R.M., Yuen R., Khoo H.E. Haemolytic activity of stonustoxin from stonefish (Synanceja horrida) venom: pore formation and the role of cationic amino acid residues. Biochem. J. 1997, 325:685-691.
19. Chetty N., Du A., Hodgson W.C., Winkel K., Fry B.G. The in vitro neuromuscular activity of Indo-Pacific sea-snake venoms: efficacy of two commercially available antivenoms. Toxicon 2004, 44:193-200.
20. Clarke A. Antarctic marine benthic diversity: patterns and processes. J. Exp. Mar. Biol. Ecol. 2008, 366:48-55.
21. Collins M.A., Rodhouse P.G.K. Southern ocean cephalopods. Adv. Mar. Biol. 2006, 50:191-265.
22. Collins M.A., Villanueva R. Taxonomy, ecology and behaviour of the cirrate octopods. Oceanogr. Mar. Biol. 2006, 44:277-322.
23. Costa J.n.d.O., Fonseca K.C., Neves Mamede C.C., Beletti M.E., Santos-Filho N.A., Soares A.M., Arantes E.C., Hirayama S.N.S., Selistre-de-Araújo H.S., Fonseca F., Henrique-Silva F.v., Penha-Silva N., Oliveira F.b.d. Bhalternin: functional and structural characterization of a new thrombin-like enzyme from Bothrops alternatus snake venom. Toxicon 2010, 55:1365-1377.
24. Cousin X., Bon S., Duval N., Massoulie J., Bon C. Cloning and expression of Acetylcholinesterase from Bungarus fasciatus venom. A new type of COOH-terminal domain; involvement of a positively charged residue in the peripheral site. J. Biol. Chem. 1996, 271:15099-15108.
25. D'Aniello A., Hakimi J., Cacace G.M., Ceccarini C. The purification and characterization of {alpha}-l-Fucosidase from the hepatopancreas of Octopus vulgaris. J. Biochem. 1982, 91:1073-1080.
26. Daly H.I. Ecology of the Antarctic Octopus Pareledone from the Scotia Sea 1996, University of Aberdeen.
27. Daly H.I., Peck L.S. Energy balance and cold adaptation in the octopus Pareledone charcoti. J. Exp. Mar. Biol. Ecol. 2000, 245:197-214.
28. Daly H.I., Rodhouse P.G. Comparative morphology of two sympatric Pareledone species from South Georgia. Antarct. Sci. 1994, 6:163-169.
29. Devaraja S., Nagaraju S., Mahadeswaraswamy Y.H., Girish K.S., Kemparaju K. A low molecular weight serine protease: purification and characterization from Hippasa agelenoides (funnel web) spider venom gland extract. Toxicon 2008, 52:130-138.
30. Dittrich B. Thermal acclimation and kinetics of a trypsin-like protease in eucarid crustaceans. J. Comp. Physiol. B. Biochem. Syst. Environ. Physiol. 1992, 162:38-46.
31. Dittrich B.U. Life under extreme conditions: aspects of evolutionary adaptation to temperature in crustacean proteases. Polar Biol. 1992, 12:269-274.
32. Duda T.F., Palumbi S.R. Molecular genetics of ecological diversification: duplication and rapid evolution of toxin genes of the venomous gastropod Conus. Proc. Natl. Acad. Sci. U.S.A. 1999, 96:6820-6823.
33. Erspamer V., Anastasi A. Structure and pharmacological actions of eledoisin, the active endecapeptide of the posterior salivary glands of Eledone. Cell. Mol. Life Sci. 1962, 18:58-59.
34. Erspamer V., Asero B. Isolation of enteramine from extracts of posterior salivary glands of Octopus vulgaris and of Discoglossus pictus skin. J. Biol. Chem. 1953, 200:311-318.
35. Fan T., Li M., Wang J., Yang L., Cong R. Purification and characterization of phenoloxidase from Octopus ocellatus. Acta Biochim. Biophys. Sin. 2009, 41:865-872.
36. Farid T.M., Tu A.T., El-Asmar M.F. Characterization of cerastobin, a thrombin-like enzyme from the venom of Cerastes vipera (Sahara sand viper). Biochemistry 1989, 28:371-377.
37. Feng L., Gao R., Gopalakrishnakone P. Isolation and characterization of a hyaluronidase from the venom of Chinese red scorpion Buthus martensi. Comp. Biochem. Physiol. C Toxicol. Pharmacol. 2008, 148:250-257.
38. Fiorito G., Gherardi F. Prey-handling behaviour of Octopus vulgaris (Mollusca, Cephalopoda) on Bivalve preys. Behav. Processes 1999, 46:75-88.
39. Fry B.G. From genome to " venome": molecular origin and evolution of the snake venom proteome inferred from phylogenetic analysis of toxin sequences and related body proteins. Genome Res. 2005, 15:403-420.
40. Fry B.G., Wüster W., Ramjan S.F.R., Jackson T., Martelli P., Kini R.M. Analysis of Colubroidea snake venoms by liquid chromatography with mass spectrometry: evolutionary and toxinological implications. Rapid Commun. Mass Spectrom. 2003, 17:2047-2062.
41. Fry B.G., Wickramaratana J.C., Lemme S., Beuve A., Garbers D., Hodgson W.C., Alewood P. Novel natriuretic peptides from the venom of the inland taipan (Oxyuranus microlepidotus): isolation, chemical and biological characterisation. Biochem. Biophys. Res. Commun. 2005, 327:1011-1015.
42. Fry B.G., Vidal N., Norman J.A., Vonk F.J., Scheib H., Ramjan S.F.R., Kuruppu S., Fung K., Blair Hedges S., Richardson M.K., Hodgson W.C., Ignjatovic V., Summerhayes R., Kochva E. Early evolution of the venom system in lizards and snakes. Nature 2006, 439:584-588.
43. Fry B.G., Scheib H., van der Weerd L., Young B., McNaughtan J., Ramjan S.F.R., Vidal N., Poelmann R.E., Norman J.A. Evolution of an Arsenal: structural and functional diversification of the venom system in the advanced snakes (Caenophidia). Mol. Cell Proteomics 2008, 7:215-246.
44. Fry B.G., Roelants K., Norman J. Tentacles of venom: toxic protein convergence in the Kingdom Animalia. J. Mol. Evol. 2009, 68:311-321.
45. Fry B.G., Wuster W. Assembling an arsenal: origin and evolution of the snake venom proteome inferred from phylogenetic analysis of toxin sequences. Mol. Biol. Evol. 2004, 21:870-883.
46. Ghiretti F. Cephalotoxin: the crab-paralysing agent of the posterior salivary glands of Cephalopods. Nature 1959, 183:1192-1193.
47. Ghiretti F. Toxicity of octopus saliva against crustacea. Ann. N.Y. Acad. Sci. 1960, 90:726-741.
48. Gibbs P.J., Greenaway P. Histological structure of the posterior salivary glands in the blue ringed octopus Hapalochlaena maculosa Hoyle. Toxicon 1978, 16:59-70.
49. Goyffon M. The venomous function. Perspectives in Molecular Toxinology 2002, 423-433. John Wiley and Sons, West Sussex. A. Ménez (Ed.).
50. Grisley M.S. Separation and partial characterization of salivary enzymes expressed during prey handling in the octopus Eledone cirrhosa. Comp. Biochem. Physiol. B. Biochem. Mol. Biol. 1993, 105:183-192.
51. Grisley M.S., Boyle P.R. Bioassay and proteolytic activity of digestive enzymes from octopus saliva. Comp. Biochem. Physiol. B. Biochem. Mol. Biol. 1987, 88:1117-1123.
52. Grisley M.S., Boyle P.R., Key L.N. Eye puncture as a route of entry for saliva during predation on crabs by the octopus Eledone cirrhosa (Lamarck). J. Exp. Mar. Biol. Ecol. 1996, 202:225-237.
53. Grisley M.S., Boyle P.R., Pierce G.J., Key L.N. Factors affecting prey handling in lesser octopus (Eledone cirrhosa) feeding on crabs (Carcinus maenas). J. Mar. Biolog. Assoc. U.K. 1999, 79:1085-1090.
54. Grubert M.A., Wadley V.A., White R.W.G. Diet and feeding strategy of Octopus maorum in southeast Tasmania. Bull. Mar. Sci. 1999, 65:441-451.
55. Guimarães L.H., Terenzi H.F., Jorge J.A., Polizeli M.L. Thermostable conidial and mycelial alkaline phosphatases from the thermophilic fungus Scytalidium thermophilum. J. Ind. Microbiol. Biotechnol. 2001, 27:265-270.
56. Gusmani L., Avian M., Galil B., Patriarca P., Rottini G. Biologically active peptides in the venom of the jellyfish Rhopilema nomadica. Toxicon 1997, 35:637-648.
57. Harvey A.L. Cytolytic toxins. Handbook of Toxinology 1990, 1-66. Marcel Dekker, New York. T.W. Shier, D. Mebs (Eds.).
58. Heatwole H., Poran N.S. Resistances of sympatric and Allopatric Eels to Sea snake venoms. Copeia 1995, 1995:136-147.
59. Heitz J.R., Norment B.R. Characteristics of an alkaline phosphatase activity in brown recluse venom. Toxicon 1974, 12:181-187.
60. Henze M. p-Oxyphanylaethylamin, das Speicheldrusengift der Cephalopoden. Hoppe-Seyler's Z. Physiol. Chem. 1913, 87:51-58.
61. 2 from Crotalus durissus terrificus (South American rattlesnake) venom. Protein J. 2005, 24:233-242.
62. Herzig V., Khalife A.A., Chong Y., Isbister G.K., Currie B.J., Churchill T.B., Horner S., Escoubas P., Nicholson G.M., Hodgson W.C. Intersexual variations in Northern (Missulena pruinosa) and Eastern (M. bradleyi) mouse spider venom. Toxicon 2008, 51:1167-1177.
63. Johnson A.J., Kline D.L., Alkjaersig N. Assay methods and standard preparations for plasmin, plasminogen and urokinase in purified systems, 1967-1968. Thromb. Diath. Haemorrh 1969, 21:259-272.
64. de Jong M.C.J.M., Kawamoto F., Bleumink E., Kloosterhuis A.J., Meijer G.T. A comparative study of the spicule venom of Euproctis caterpillars. Toxicon 1982, 20:477-485.
65. Kanda A., Iwakoshi-Ukena E., Takuwa-Kuroda K., Minakata H. Isolation and characterization of novel tachykinins from the posterior salivary gland of the common octopus Octopus vulgaris. Peptides 2003, 24:35-43.
66. Key L.N., Boyle P.R., Jaspars M. Novel activities of saliva from the octopus Eledone cirrhosa (Mollusca; Cephalopoda). Toxicon 2002, 40:677-683.
67. Kordis D., Gubensek F. Adaptive evolution of animal toxin multigene families. Gene 2000, 261:43-52.
68. Kubodera T., Okutani T. New and rare Cephalopods from the Antarctic waters. Mem. Natl. Inst. Polar Res. 1986, 129-143. Spec. Issue.
69. Lewis J.G.E. The Biology of Centipedes 1981, Cambridge University Press, Cambridge.
70. Lewis R.J., Garcia M.L. Therapeutic potential of venom peptides. Nat. Rev. Drug Discov. 2003, 2:790-802.
71. Li C., Yu H., Liu S., Xing R., Guo Z., Li P. Factors affecting the protease activity of venom from jellyfish Rhopilema esculentum Kishinouye. Bioorg. Med. Chem. Lett. 2005, 15:5370-5374.
72. 2 toxins. Mol. Biol. Evol. 2005, 22:934-941.
73. Liu S., Sun M.-Z., Greenaway F.T. A novel plasminogen activator from Agkistrodon blomhoffii Ussurensis venom (ABUSV-PA): purification and characterization. Biochem. Biophys. Res. Commun. 2006, 348:1279-1287.
74. Liu S., Sun M.-Z., Sun C., Zhao B., Greenaway F.T., Zheng Q. A novel serine protease from the snake venom of Agkistrodon blomhoffii ussurensis. Toxicon 2008, 52:760-768.
75. Lu J., Yang H., Yu H., Gao W., Lai R., Liu J., Liang X. A novel serine protease inhibitor from Bungarus fasciatus venom. Peptides 2008, 29:369-374.
76. Lu C.C., Stranks T.N. Synopsis of Pareledone and Megaleledone species, with description of two new species from East Antarctica (Cephalopoda: Octopodidae). Mem. Mus. Vic. 1994, 54:221-242.
77. Magalhães M.R., da Silva N.J., Ulhoa C.J. A hyaluronidase from Potamotrygon motoro (freshwater stingrays) venom: isolation and characterization. Toxicon 2008, 51:1060-1067.
78. Mather J.A. Foraging, feeding and prey remains in middens of juvenile Octopus vulgaris (Mollusca: Cephalopoda). J. Zool. 1991, 224:27-39.
79. Matsui T., Fujimura Y., Titani K. Snake venom proteases affecting hemostasis and thrombosis. Biochim. Biophys. Acta Protein Struct. Mol. Enzym. 2000, 1477:146-156.
80. McComb R.B., Bowers G.N.J., Posen S. Alkaline Phosphatase 1979, Plenum Press, New York.
81. McDonald N.M., Cottrell G.A. Purification and mode of action of toxin from Eledone cirrosa. Comp. Gen. Pharmacol. 1972, 3:243-248.
82. McIntosh J.M., Ghomashchi F., Gelb M.H., Dooley D.J., Stoehr S.J., Giordani A.B., Naisbitt S.R., Olivera B.M. Conodipine-M, a novel Phospholipase A(2) isolated from the venom of the marine snail Conus magus. J. Biol. Chem. 1995, 270:3518-3526.
83. Messenger J.B., Young J.Z. The radular apparatus of cephalopods. Philos. Trans. R. Soc. Lond., B. Biol. Sci. 1999, 354:161-182.
84. Morey S.S., Kiran K.M., Gadag J.R. Purification and properties of hyaluronidase from Palamneus gravimanus (Indian black scorpion) venom. Toxicon 2006, 47:188-195.
85. Morishita T. Participation in digestion by the proteolytic enzymes of the posterior salivary gland in Octopus-II Isolation and purification of the proteolytic enzymes from the posterior salivary gland. Nippon Suisan Gakkai Shi 1974, 40:601-607.
86. Nakar O., Ovadia M., Kochva E. Isolation and characterization of a proteolytic factor from the venom of Vipera palaestinae. Toxicon 1986, 24:293-304.
87. Nixon M. Is there external digestion by Octopus?. J. Zool. 1984, 202:441-447.
88. Ohno M., Ogawa T., Oda-Ueda N., Chijiwa T., Hattori S. Accelerated and regional evolution of snake venom gland isozymes. Perspectives in Molecular Toxinology 2002, 387-400. Wiley and Sons, West Sussex. A. Ménez (Ed.).
89. Pandya B.V., Budzynski A.Z. Anticoagulant proteases from western diamondback rattlesnake (Crotalus atrox) venom. Biochemistry 1984, 23:460-470.
90. Piatkowski U., Allcock A.L., Vecchione M. Cephalopod diversity and ecology. Berichte-zur-Polar-und-Meeresforschung 2003, 470:32-38.
91. Pilson M.E.Q., Taylor P.B. Hole drilling by octopus. Science 1961, 134:1366-1368.
92. Poh C.H., Yuen R., Chung M.C.M., Khoo H.E. Purification and partial characterization of hyaluronidase from stonefish (Synanceja horrida) venom. Comp. Biochem. Physiol. Part B. Comp. Biochem. 1992, 101:159-163.
93. Quinn D.M. Acetylcholinesterase: enzyme structure, reaction dynamics, and virtual transition states. Chem. Rev. 1987, 87:955-979.
94. Risch M., Georgieva D.N., von Bergen M., Jehmlich N., Genov N., Arni R.K., Betzel C. Snake venomics of the Siamese Russell's viper (Daboia russelli siamensis) - relation to pharmacological activities. J. Proteomics 2009, 72:256-269.
95. Roach J.C. A clade of trypsins found in cold-adapted fish. Proteins: Struct. Funct. Genet. 2002, 47:31-44.
96. Robertson S.S.D., Delpierre G.R. Studies on African snake venoms - IV Some enzymatic activities in the venom of the boomslang Dispholidus typus. Toxicon 1969, 7:189-194.
97. Rodrigues V.M., Soares A.M., Guerra-S· R., Rodrigues V., Fontes M.R.M., Giglio J.R. Structural and functional characterization of Neuwiedase, a nonhemorrhagic fibrin(ogen)olytic metalloprotease from Bothrops neuwiedi snake venom. Arch. Biochem. Biophys. 2000, 381:213-224.
98. Rodrigues M.C.A., Guimarães L.H.S., Liberato J.L., Polizeli M.d.L.T.d.M., dos Santos W.F. Acid and alkaline phosphatase activities of a fraction isolated from Parawixia bistriata spider venom. Toxicon 2006, 47:854-858.
99. Romero-Saravia O., Hamdorf K. Alkaline phosphatase. A dominant enzyme of microvillus structure of cephalopod photoreceptors. Biochim. Biophys. Acta Biomembr. 1983, 729:90-96.
100. Rosenberg P. Phospholipases. Handbook of Toxinology 1990, 67-278. Marcel Dekker, New York. T.W. Shier, D. Mebs (Eds.).
101. Runham N.W., Bailey C.J., Carr M., Evans C.A., Malham S. Hole drilling in crab and gastropod shells by Eledone cirrhosa (Lamarck, 1798). Sci. Mar. 1997, 61:67-76.
102. Sales P.B.V., Santoro M.L. Nucleotidase and DNase activities in Brazilian snake venoms. Comp. Biochem. Physiol. C Toxicol. Pharmacol. 2008, 147:85-95.
103. Sazima I., Bastos-de-Almeida L. The bird kraken: octopus preys on a sea bird at an oceanic island in the tropical west Atlantic. J. Mar. Biolog. Assoc. U.K., Biodiv. Rec. 2006.
104. Sheumack D.D., Howden M.E.H., Spence I., Quinn R.J. Maculotoxin: a neurotoxin from the venom glands of the octopus Hapalochlaena maculosa identified as tetrodotoxin. Science 1978, 199:188-189.
105. Songdahl J.H., Shapiro B.I. Purification and composition of a toxin from the posterior salivary gland of Octopus dofleini. Toxicon 1974, 12:109-112.
106. Strugnell J., Norman M., Jackson J., Drummond A.J., Cooper A. Molecular phylogeny of coleoid cephalopods (Mollusca: Cephalopoda) using a multigene approach; the effect of data partitioning on resolving phylogenies in a Bayesian framework. Mol. Phylogenet. Evol. 2005, 37:426-441.
107. Strugnell J., Rogers A.D., Prodöhl P.A., Collins M.A., Allcock A.L. The thermohaline expressway: the Southern Ocean as a centre of origin for deep-sea octopuses. Cladistics 2008, 24:853-860.
108. Sulkowski E., Björk W., Laskowski M. A specific and nonspecific alkaline monophosphatase in the venom of Bothrops atrox and their occurrence in the purified venom phosphodiesterase. J. Biol. Chem. 1963, 238:2477-2486.
109. 2 from Agkistrodon blomhoffii ussurensis venom: purification, proteomic, functional and structural characterizations. Biochimie 2009, 91:558-567.
110. 2. J. Immunol. Methods 1980, 32:185-193.
111. Tu A.T., Chua A. Acid and alkaline phosphomonoesterase activities in snake venoms. Comp. Biochem. Physiol. 1966, 17:297-307.
112. Undheim E.A.B., Norman J.A., Thoen H.H., Fry B.G. Identification of Southern Ocean octopod samples using mtCOI. CR Biol. 2010, 333:395-404.
113. Valério A.A., Corradini A.C., Panunto P.C., Mello S.M., Hyslop S. Purification and characterization of a phosphodiesterase from Bothrops alternatus snake venom. J. Protein Chem. 2002, 21:495-503.
114. Vecchione M.M., Piatkowski M.U., Allcock M.A. Biology of the cirrate octopod Grimpoteuthis glacialis (Cephalopoda: Opisthoteuthidae) in the South Shetland islands, Antarctica. S. Afr. J. Mar. Sci. 1998, 20:421-428.
115. Vetter R.S. Medical aspects of spider bites. Annu. Rev. Entomol. 2008, 53:409.
116. 2 myotoxin from the venom of the Irian Jayan death adder (Acanthophis rugosus). Br. J. Pharmacol. 2003, 138:333-342.
117. Yotsu-Yamashita M., Mebs D., Flachsenberger W. Distribution of tetrodotoxin in the body of the blue-ringed octopus (Hapalochlaena maculosa). Toxicon 2007, 49:410-412.
118. Zhou X., Tan T.-C., Valiyaveettil S., Go M.L., Manjunatha Kini R., Velazquez-Campoy A., Sivaraman J. Structural characterization of myotoxic Ecarpholin S from Echis carinatus venom. Biophys. J. 2008, 95:3366-3380.
119. Zhu J.-y., Ye G.-y., Hu C. Molecular cloning and characterization of acid phosphatase in venom of the endoparasitoid wasp Pteromalus puparum (Hymenoptera: Pteromalidae). Toxicon 2008, 51:1391-1399.