Characterization of the hydrophobic substrate-binding site of the bacterial beta class glutathione transferase from Proteus mirabilis

Protein Engineering, Design and Selection

Volumn 23, Issue 9, 2010, Pages 743-750

  Federici, Luca ^a   Masulli, Michele ^a   Di Ilio, Carmine ^a   Allocati, Nerino ^a  

^a UNIVERSITY OF CHIETI   (Italy)

Author keywords

bacterial beta class glutathione transferase; detoxification; H site; hydrophobic binding site; TNS,2 (p toluidino) 6 naphthalenesulfonic acid

Indexed keywords

CATALYSE; CATALYTIC MECHANISMS; CATALYTIC PROPERTIES; CO-SUBSTRATE; COSUBSTRATES; GLUTATHIONE TRANSFERASE; GLUTATHIONES; H-SITE; HYDROPHOBIC BINDING; HYDROPHOBIC SUBSTRATE; OVERALL STABILITIES; PROTEUS MIRABILIS; SINGLE RESIDUE; SITE DIRECTED MUTAGENESIS; TNS,2-(P-TOLUIDINO)-6-NAPHTHALENESULFONIC ACID;

ACIDS; BACTERIOLOGY; BINDING ENERGY; BIOREMEDIATION; CATALYSIS; DETOXIFICATION; ENZYMES; HYDROPHOBICITY;

SUBSTRATES;

GLUTATHIONE TRANSFERASE;

ARTICLE; BINDING SITE; CATALYSIS; ENZYME ACTIVITY; ENZYME BINDING; HYDROPHOBICITY; NONHUMAN; PRIORITY JOURNAL; PROTEUS MIRABILIS; SITE DIRECTED MUTAGENESIS;

BACTERIAL PROTEINS; BENZENE DERIVATIVES; BINDING SITES; CIRCULAR DICHROISM; ENZYME STABILITY; GLUTATHIONE; GLUTATHIONE TRANSFERASE; HYDROGEN-ION CONCENTRATION; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROTEIN FOLDING; PROTEUS MIRABILIS; TEMPERATURE;

BACTERIA (MICROORGANISMS); PROTEUS MIRABILIS;

EID: 77956029069     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzq048     Document Type: Article

References (30)

1. Agianian,B., Tucker,P.A., Schouten,A., Leonard,K., Bullard,B. and Gros,P. (2003) J. Mol. Biol., 326, 151-165.
2. Allocati,N., Favaloro,B., Masulli,M., Alexeyev,M.F. and Di Ilio,C. (2003) Biochem. J., 373, 305-311.
3. Allocati,N., Masulli,M., Pietracupa,M., Favaloro,B., Federici,L. and Di Ilio,C. (2005) Biochem. J., 385, 37-43.
4. Allocati,N., Federici,L., Masulli,M., Favaloro,B. and Di Ilio,C. (2008) Proteins, 71, 16-23.
5. Allocati,N., Federici,L., Masulli,M. and Di Ilio,C. (2009) FEBS J., 276, 58-75.
6. Armstrong,R.N. (1991) Chem. Res. Toxicol., 4, 131-140.
7. Armstrong,R.N. (1997) Chem. Res. Toxicol., 10, 2-18.
8. Bradford,M.M. (1976) Anal. Biochem., 72, 248-254.
9. De Luca,A., Favaloro,B., Carletti,E., Sacchetta,P. and Di Ilio,C. (2003) Biochem. J., 373, 539-545.
10. Di Ilio,C., Aceto,A., Piccolomini,R., Allocati,N., Faraone,A., Cellini,L., Ravagnan,G. and Federici,G. (1988) Biochem. J., 255, 971-975.
11. Ellis,K.J. and Morrison,J.F. (1982) Methods Enzymol., 87, 405-426.
12. Federici,L., Masulli,M., Bonivento,D., Di Matteo,A., Gianni,S., Favaloro,B., Di Ilio,C. and Allocati,N. (2007) Biochem. J., 403, 267-274.
13. Federici,L., Lo Sterzo,C., Pezzola,S., Di Matteo,A., Scaloni,F., Federici,G. and Caccuri,A.M. (2009a) Cancer Res., 69, 8025-8034.
14. Federici,L., Masulli,M., Gianni,S., Di Ilio,C. and Allocati,N. (2009b) Biochem. Biophys. Res. Commun., 382, 525-529.
15. Habig,W.H. and Jakoby,W.B. (1981) Methods Enzymol., 77, 398-405.
16. Hayes,J.D., Flanagan,J.U. and Jowsey,I.R. (2005) Annu. Rev. Pharmacol. Toxicol., 45, 51-88.
17. Kuhnert,D.C., Sayed,Y., Mosebi,S., Sayed,M., Sewell,T. and Dirr,H.W. (2005) J. Mol. Biol., 349, 825-838.
18. Labrou,N.E., Kotzia,G.A. and Clonis,Y.D. (2004) Protein Eng. Des. Sel., 17, 741-748.
19. Laemmli,U.K. (1970) Nature (London), 227, 680-685.
20. Lerksuthirat,T. and Ketterman,A.J. (2008) Arch. Biochem. Biophys., 479, 97-103.
21. Lo Piero,A.R., Mercurio,V., Puglisi,I. and Petrone,G. (2010) FEBS J., 277, 255-262.
22. McGuinness,M., Ivory,C., Gilmartin,N. and Dowling,D.N. (2006) Int. Biodeter. Biodegrad., 58, 203-208.
23. Nishida,M., Harada,S., Noguchi,S., Satow,Y., Inoue,H. and Takahashi,K. (1998) J. Mol. Biol., 281, 135-147.
24. Oakley,A.J. (2005) Curr. Opin. Struct. Biol., 15, 716-723.
25. Perito,B., Allocati,N., Casalone,E., Masulli,M., Dragani,B., Polsinelli,M., Aceto,A. and Di Ilio,C. (1996) Biochem. J., 318, 157-162.
26. Prade,L., Huber,R. and Bieseler,B. (1998) Structure, 6, 1445-1452.
27. Ricci,G., Caccuri,A.M., Lo Bello,M., Rosato,N., Mei,G., Nicotra,M., Chiessi,E., Mazzetti,A.P. and Federici,G. (1996) J. Biol. Chem., 271, 16187-16192.
28. Rossjohn,J., Polekhina,G., Feil,S.C., Allocati,N., Masulli,M., Di Ilio,C. and Parker,M.W. (1998) Structure, 6, 721-734.
29. Sheehan,D., Meade,G., Foley,V.M. and Dowd,C.A. (2001) Biochem. J., 360, 1-16.
30. Tocheva,E.I., Fortin,P.D., Eltis,L.D. and Murphy,M.E.P. (2006) J. Biol. Chem., 281, 30933-30940.